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O14810

- CPLX1_HUMAN

UniProt

O14810 - CPLX1_HUMAN

Protein

Complexin-1

Gene

CPLX1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Positively regulates a late step in synaptic vesicle exocytosis. Organizes the SNAREs into a cross-linked zigzag topology that, when interposed between the vesicle and plasma membranes, is incompatible with fusion, thereby preventing SNAREs from releasing neurotransmitters until an action potential arrives at the synapse. Also involved in glucose-induced secretion of insulin by pancreatic beta-cells By similarity.By similarity

    GO - Molecular functioni

    1. neurotransmitter transporter activity Source: Ensembl

    GO - Biological processi

    1. exocytosis Source: ProtInc
    2. glutamate secretion Source: Reactome
    3. insulin secretion Source: Ensembl
    4. neurotransmitter secretion Source: Reactome
    5. synaptic transmission Source: Reactome
    6. synaptic vesicle exocytosis Source: Ensembl

    Keywords - Biological processi

    Exocytosis, Neurotransmitter transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_12591. Glutamate Neurotransmitter Release Cycle.
    REACT_15293. Dopamine Neurotransmitter Release Cycle.
    REACT_15309. Acetylcholine Neurotransmitter Release Cycle.
    REACT_15418. Norepinephrine Neurotransmitter Release Cycle.
    REACT_15425. Serotonin Neurotransmitter Release Cycle.
    REACT_23947. GABA synthesis, release, reuptake and degradation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Complexin-1
    Alternative name(s):
    Complexin I
    Short name:
    CPX I
    Synaphin-2
    Gene namesi
    Name:CPLX1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:2309. CPLX1.

    Subcellular locationi

    Cytoplasmcytosol By similarity
    Note: Enriched at synaptic-releasing sites in mature neurons.By similarity

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. dendrite Source: Ensembl
    3. neuronal cell body Source: Ensembl
    4. synapse Source: Ensembl
    5. synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26826.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 134134Complexin-1PRO_0000144870Add
    BLAST

    Proteomic databases

    MaxQBiO14810.
    PaxDbiO14810.
    PeptideAtlasiO14810.
    PRIDEiO14810.

    PTM databases

    PhosphoSiteiO14810.

    Expressioni

    Tissue specificityi

    Nervous system. In hippocampus and cerebellum, expressed mainly by inhibitory neurons. Overexpressed in substantia nigra from patients with Parkinson disease.3 Publications

    Gene expression databases

    ArrayExpressiO14810.
    BgeeiO14810.
    CleanExiHS_CPLX1.
    GenevestigatoriO14810.

    Interactioni

    Subunit structurei

    Binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A.1 Publication

    Protein-protein interaction databases

    BioGridi116028. 32 interactions.
    DIPiDIP-56109N.
    IntActiO14810. 2 interactions.
    STRINGi9606.ENSP00000305613.

    Structurei

    Secondary structure

    1
    134
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi29 – 7042

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3RK3X-ray3.50E26-83[»]
    3RL0X-ray3.80g/h/i/j/k/l/m/n26-83[»]
    ProteinModelPortaliO14810.
    SMRiO14810. Positions 32-72.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni48 – 7023Interaction with the SNARE complexBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili29 – 6941Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the complexin/synaphin family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG85303.
    HOGENOMiHOG000017716.
    HOVERGENiHBG062792.
    KOiK15294.
    OMAiKYARMEA.
    OrthoDBiEOG71G9XG.
    PhylomeDBiO14810.
    TreeFamiTF315172.

    Family and domain databases

    InterProiIPR008849. Synaphin.
    [Graphical view]
    PANTHERiPTHR16705. PTHR16705. 1 hit.
    PfamiPF05835. Synaphin. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O14810-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEFVMKQALG GATKDMGKML GGDEEKDPDA AKKEEERQEA LRQAEEERKA    50
    KYAKMEAERE AVRQGIRDKY GIKKKEEREA EAQAAMEANS EGSLTRPKKA 100
    IPPGCGDEVE EEDESILDTV IKYLPGPLQD MLKK 134
    Length:134
    Mass (Da):15,030
    Last modified:January 1, 1998 - v1
    Checksum:iA7A52F17F10D28A4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF022383 mRNA. Translation: AAB72108.1.
    KJ534815 mRNA. Translation: AHW56455.1.
    BT007029 mRNA. Translation: AAP35676.1.
    AB593095 mRNA. Translation: BAJ84035.1.
    AK311921 mRNA. Translation: BAG34862.1.
    AC139887 Genomic DNA. No translation available.
    CH471131 Genomic DNA. Translation: EAW82648.1.
    CH471131 Genomic DNA. Translation: EAW82649.1.
    BC002471 mRNA. Translation: AAH02471.1.
    CCDSiCCDS46995.1.
    RefSeqiNP_006642.1. NM_006651.3.
    UniGeneiHs.478930.

    Genome annotation databases

    EnsembliENST00000304062; ENSP00000305613; ENSG00000168993.
    GeneIDi10815.
    KEGGihsa:10815.
    UCSCiuc003gbi.3. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF022383 mRNA. Translation: AAB72108.1 .
    KJ534815 mRNA. Translation: AHW56455.1 .
    BT007029 mRNA. Translation: AAP35676.1 .
    AB593095 mRNA. Translation: BAJ84035.1 .
    AK311921 mRNA. Translation: BAG34862.1 .
    AC139887 Genomic DNA. No translation available.
    CH471131 Genomic DNA. Translation: EAW82648.1 .
    CH471131 Genomic DNA. Translation: EAW82649.1 .
    BC002471 mRNA. Translation: AAH02471.1 .
    CCDSi CCDS46995.1.
    RefSeqi NP_006642.1. NM_006651.3.
    UniGenei Hs.478930.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3RK3 X-ray 3.50 E 26-83 [» ]
    3RL0 X-ray 3.80 g/h/i/j/k/l/m/n 26-83 [» ]
    ProteinModelPortali O14810.
    SMRi O14810. Positions 32-72.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 116028. 32 interactions.
    DIPi DIP-56109N.
    IntActi O14810. 2 interactions.
    STRINGi 9606.ENSP00000305613.

    PTM databases

    PhosphoSitei O14810.

    Proteomic databases

    MaxQBi O14810.
    PaxDbi O14810.
    PeptideAtlasi O14810.
    PRIDEi O14810.

    Protocols and materials databases

    DNASUi 10815.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000304062 ; ENSP00000305613 ; ENSG00000168993 .
    GeneIDi 10815.
    KEGGi hsa:10815.
    UCSCi uc003gbi.3. human.

    Organism-specific databases

    CTDi 10815.
    GeneCardsi GC04M000778.
    HGNCi HGNC:2309. CPLX1.
    MIMi 605032. gene.
    neXtProti NX_O14810.
    PharmGKBi PA26826.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG85303.
    HOGENOMi HOG000017716.
    HOVERGENi HBG062792.
    KOi K15294.
    OMAi KYARMEA.
    OrthoDBi EOG71G9XG.
    PhylomeDBi O14810.
    TreeFami TF315172.

    Enzyme and pathway databases

    Reactomei REACT_12591. Glutamate Neurotransmitter Release Cycle.
    REACT_15293. Dopamine Neurotransmitter Release Cycle.
    REACT_15309. Acetylcholine Neurotransmitter Release Cycle.
    REACT_15418. Norepinephrine Neurotransmitter Release Cycle.
    REACT_15425. Serotonin Neurotransmitter Release Cycle.
    REACT_23947. GABA synthesis, release, reuptake and degradation.

    Miscellaneous databases

    GeneWikii CPLX1.
    GenomeRNAii 10815.
    NextBioi 41085.
    PROi O14810.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O14810.
    Bgeei O14810.
    CleanExi HS_CPLX1.
    Genevestigatori O14810.

    Family and domain databases

    InterProi IPR008849. Synaphin.
    [Graphical view ]
    PANTHERi PTHR16705. PTHR16705. 1 hit.
    Pfami PF05835. Synaphin. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complexins: cytosolic proteins that regulate SNAP receptor function."
      McMahon H.T., Missler M., Li C., Suedhof T.C.
      Cell 83:111-119(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism."
      Corominas R., Yang X., Lin G.N., Kang S., Shen Y., Ghamsari L., Broly M., Rodriguez M., Tam S., Wanamaker S.A., Fan C., Yi S., Tasan M., Lemmens I., Kuang X., Zhao N., Malhotra D., Michaelson J.J.
      , Vacic V., Calderwood M.A., Roth F.P., Tavernier J., Horvath S., Salehi-Ashtiani K., Korkin D., Sebat J., Hill D.E., Hao T., Vidal M., Iakoucheva L.M.
      Nat. Commun. 5:3650-3650(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Fetal brain.
    3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "Full-length transcriptome analysis of human retina-derived cell lines ARPE-19 and Y79 using the vector-capping method."
      Oshikawa M., Tsutsui C., Ikegami T., Fuchida Y., Matsubara M., Toyama S., Usami R., Ohtoko K., Kato S.
      Invest. Ophthalmol. Vis. Sci. 52:6662-6670(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Cerebellum.
    6. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.
    9. "Preferential involvement of excitatory neurons in medial temporal lobe in schizophrenia."
      Harrison P.J., Eastwood S.L.
      Lancet 352:1669-1673(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    10. "Cerebellar synaptic protein expression in schizophrenia."
      Eastwood S.L., Cotter D., Harrison P.J.
      Neuroscience 105:219-229(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    11. "Proteome analysis of human substantia nigra in Parkinson's disease."
      Basso M., Giraudo S., Corpillo D., Bergamasco B., Lopiano L., Fasano M.
      Proteomics 4:3943-3952(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.
    12. Cited for: X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS) OF 28-83 ALONE AND IN COMPLEX WITH SNAP25; VAMP2 AND RAT STX1A, SUBUNIT, FUNCTION.

    Entry informationi

    Entry nameiCPLX1_HUMAN
    AccessioniPrimary (citable) accession number: O14810
    Secondary accession number(s): A6NI80
    , B2R4R5, D3DVN3, F1T0G1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3