O14810 (CPLX1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Complexin-1

Alternative name(s):
Complexin I
Short name=CPX I
Synaphin-2

Gene names

Name:

CPLX1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	134 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Positively regulates a late step in synaptic vesicle exocytosis. Organizes the SNAREs into a cross-linked zigzag topology that, when interposed between the vesicle and plasma membranes, is incompatible with fusion, thereby preventing SNAREs from releasing neurotransmitters until an action potential arrives at the synapse. Also involved in glucose-induced secretion of insulin by pancreatic beta-cells By similarity. Ref.10
Subunit structure	Binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A. Ref.10
Subcellular location	Cytoplasm › cytosol By similarity. Note: Enriched at synaptic-releasing sites in mature neurons By similarity.
Tissue specificity	Nervous system. In hippocampus and cerebellum, expressed mainly by inhibitory neurons. Overexpressed in substantia nigra from patients with Parkinson disease. Ref.7 Ref.8 Ref.9
Sequence similarities	Belongs to the complexin/synaphin family.

Ontologies

Keywords
Biological process	Exocytosis Neurotransmitter transport Transport
Cellular component	Cytoplasm
Domain	Coiled coil
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glutamate secretion Traceable author statement. Source: Reactome
Cellular component	cytosol Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 134

134

Complexin-1

PRO_0000144870

Regions

Region

48 – 70

23

Interaction with the SNARE complex By similarity

Coiled coil

29 – 69

41

Potential

Secondary structure

1

.

.

.

134

Helix Strand Turn

Details...

Helix

29 – 70

42

Sequences

	Sequence		Length	Mass (Da)
	O14810 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: A7A52F17F10D28A4	FASTA	134	15,030
	10 20 30 40 50 60 MEFVMKQALG GATKDMGKML GGDEEKDPDA AKKEEERQEA LRQAEEERKA KYAKMEAERE 70 80 90 100 110 120 AVRQGIRDKY GIKKKEEREA EAQAAMEANS EGSLTRPKKA IPPGCGDEVE EEDESILDTV 130 IKYLPGPLQD MLKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complexins: cytosolic proteins that regulate SNAP receptor function." McMahon H.T., Missler M., Li C., Suedhof T.C. Cell 83:111-119(1995) [PubMed: 7553862] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cerebellum.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.
[7]	"Preferential involvement of excitatory neurons in medial temporal lobe in schizophrenia." Harrison P.J., Eastwood S.L. Lancet 352:1669-1673(1998) [PubMed: 9853440] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"Cerebellar synaptic protein expression in schizophrenia." Eastwood S.L., Cotter D., Harrison P.J. Neuroscience 105:219-229(2001) [PubMed: 11483314] [Abstract] Cited for: TISSUE SPECIFICITY.
[9]	"Proteome analysis of human substantia nigra in Parkinson's disease." Basso M., Giraudo S., Corpillo D., Bergamasco B., Lopiano L., Fasano M. Proteomics 4:3943-3952(2004) [PubMed: 15526345] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.
[10]	"Complexin cross-links prefusion SNAREs into a zigzag array." Kummel D., Krishnakumar S.S., Radoff D.T., Li F., Giraudo C.G., Pincet F., Rothman J.E., Reinisch K.M. Nat. Struct. Mol. Biol. 18:927-933(2011) [PubMed: 21785414] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS) OF 28-83 ALONE AND IN COMPLEX WITH SNAP25; VAMP2 AND RAT STX1A, SUBUNIT, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF022383 mRNA. Translation: AAB72108.1.
BT007029 mRNA. Translation: AAP35676.1.
AK311921 mRNA. Translation: BAG34862.1.
AC139887 Genomic DNA. No translation available.
CH471131 Genomic DNA. Translation: EAW82648.1.
CH471131 Genomic DNA. Translation: EAW82649.1.
BC002471 mRNA. Translation: AAH02471.1.

IPI

IPI00396023.

RefSeq

NP_006642.1. NM_006651.3.

UniGene

Hs.478930.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3RK3	X-ray	3.50	E	28-83	[»]
3RL0	X-ray	3.80	g/h/i/j/k/l/m/n	28-83	[»]

ProteinModelPortal

O14810.

SMR

O14810. Positions 32-72.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-56109N.

IntAct

O14810. 2 interactions.

STRING

O14810.

PTM databases

PhosphoSite

O14810.

Proteomic databases

PeptideAtlas

O14810.

PRIDE

O14810.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000304062; ENSP00000305613; ENSG00000168993.

GeneID

10815.

KEGG

hsa:10815.

UCSC

uc003gbi.1. human.

Organism-specific databases

CTD

10815.

GeneCards

GC04M000778.

H-InvDB

HIX0004006.

HGNC

HGNC:2309. CPLX1.

MIM

605032. gene.

neXtProt

NX_O14810.

PharmGKB

PA26826.

GenAtlas

Search...

Phylogenomic databases

GeneTree

ENSGT00430000033109.

HOVERGEN

HBG062792.

OMA

SIVDTVM.

OrthoDB

EOG44MXTP.

Enzyme and pathway databases

Reactome

REACT_13685. Neuronal System.

Gene expression databases

ArrayExpress

O14810.

Bgee

O14810.

CleanEx

HS_CPLX1.

Genevestigator

O14810.

Family and domain databases

InterPro

IPR008849. Synaphin.
[Graphical view]

KO

K15294.

PANTHER

PTHR16705. Synaphin. 1 hit.

Pfam

PF05835. Synaphin. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

41085.

SOURCE

Search...

Entry information

Entry name

CPLX1_HUMAN

Accession

Primary (citable) accession number: O14810
Secondary accession number(s): A6NI80, B2R4R5, D3DVN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.