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Protein

Complexin-1

Gene

CPLX1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Positively regulates a late step in synaptic vesicle exocytosis. Organizes the SNAREs into a cross-linked zigzag topology that, when interposed between the vesicle and plasma membranes, is incompatible with fusion, thereby preventing SNAREs from releasing neurotransmitters until an action potential arrives at the synapse. Also involved in glucose-induced secretion of insulin by pancreatic beta-cells (By similarity).By similarity

GO - Molecular functioni

  1. neurotransmitter transporter activity Source: Ensembl

GO - Biological processi

  1. exocytosis Source: ProtInc
  2. glutamate secretion Source: Reactome
  3. insulin secretion Source: Ensembl
  4. neurotransmitter secretion Source: Reactome
  5. regulation of exocytosis Source: ParkinsonsUK-UCL
  6. synaptic transmission Source: Reactome
  7. synaptic vesicle exocytosis Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Neurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiREACT_12591. Glutamate Neurotransmitter Release Cycle.
REACT_15293. Dopamine Neurotransmitter Release Cycle.
REACT_15309. Acetylcholine Neurotransmitter Release Cycle.
REACT_15418. Norepinephrine Neurotransmitter Release Cycle.
REACT_15425. Serotonin Neurotransmitter Release Cycle.
REACT_23947. GABA synthesis, release, reuptake and degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Complexin-1
Alternative name(s):
Complexin I
Short name:
CPX I
Synaphin-2
Gene namesi
Name:CPLX1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 4

Organism-specific databases

HGNCiHGNC:2309. CPLX1.

Subcellular locationi

Cytoplasmcytosol By similarity
Note: Enriched at synaptic-releasing sites in mature neurons.By similarity

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. dendrite Source: Ensembl
  3. neuronal cell body Source: Ensembl
  4. synapse Source: Ensembl
  5. synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex Source: Ensembl
  6. synaptobrevin 2-SNAP-25-syntaxin-3-complexin complex Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26826.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 134134Complexin-1PRO_0000144870Add
BLAST

Proteomic databases

MaxQBiO14810.
PaxDbiO14810.
PeptideAtlasiO14810.
PRIDEiO14810.

PTM databases

PhosphoSiteiO14810.

Expressioni

Tissue specificityi

Nervous system. In hippocampus and cerebellum, expressed mainly by inhibitory neurons. Overexpressed in substantia nigra from patients with Parkinson disease.3 Publications

Gene expression databases

BgeeiO14810.
CleanExiHS_CPLX1.
ExpressionAtlasiO14810. baseline and differential.
GenevestigatoriO14810.

Interactioni

Subunit structurei

Binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A.1 Publication

Protein-protein interaction databases

BioGridi116028. 34 interactions.
DIPiDIP-56109N.
IntActiO14810. 2 interactions.
STRINGi9606.ENSP00000305613.

Structurei

Secondary structure

1
134
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi29 – 7042Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3RK3X-ray3.50E26-83[»]
3RL0X-ray3.80g/h/i/j/k/l/m/n26-83[»]
ProteinModelPortaliO14810.
SMRiO14810. Positions 32-72.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni48 – 7023Interaction with the SNARE complexBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili29 – 6941Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the complexin/synaphin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG85303.
GeneTreeiENSGT00490000043416.
HOGENOMiHOG000017716.
HOVERGENiHBG062792.
InParanoidiO14810.
KOiK15294.
OMAiTVMKFIP.
OrthoDBiEOG71G9XG.
PhylomeDBiO14810.
TreeFamiTF315172.

Family and domain databases

InterProiIPR008849. Synaphin.
[Graphical view]
PANTHERiPTHR16705. PTHR16705. 1 hit.
PfamiPF05835. Synaphin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O14810-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEFVMKQALG GATKDMGKML GGDEEKDPDA AKKEEERQEA LRQAEEERKA
60 70 80 90 100
KYAKMEAERE AVRQGIRDKY GIKKKEEREA EAQAAMEANS EGSLTRPKKA
110 120 130
IPPGCGDEVE EEDESILDTV IKYLPGPLQD MLKK
Length:134
Mass (Da):15,030
Last modified:January 1, 1998 - v1
Checksum:iA7A52F17F10D28A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022383 mRNA. Translation: AAB72108.1.
KJ534815 mRNA. Translation: AHW56455.1.
BT007029 mRNA. Translation: AAP35676.1.
AB593095 mRNA. Translation: BAJ84035.1.
AK311921 mRNA. Translation: BAG34862.1.
AC139887 Genomic DNA. No translation available.
CH471131 Genomic DNA. Translation: EAW82648.1.
CH471131 Genomic DNA. Translation: EAW82649.1.
BC002471 mRNA. Translation: AAH02471.1.
CCDSiCCDS46995.1.
RefSeqiNP_006642.1. NM_006651.3.
UniGeneiHs.478930.

Genome annotation databases

EnsembliENST00000304062; ENSP00000305613; ENSG00000168993.
GeneIDi10815.
KEGGihsa:10815.
UCSCiuc003gbi.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022383 mRNA. Translation: AAB72108.1.
KJ534815 mRNA. Translation: AHW56455.1.
BT007029 mRNA. Translation: AAP35676.1.
AB593095 mRNA. Translation: BAJ84035.1.
AK311921 mRNA. Translation: BAG34862.1.
AC139887 Genomic DNA. No translation available.
CH471131 Genomic DNA. Translation: EAW82648.1.
CH471131 Genomic DNA. Translation: EAW82649.1.
BC002471 mRNA. Translation: AAH02471.1.
CCDSiCCDS46995.1.
RefSeqiNP_006642.1. NM_006651.3.
UniGeneiHs.478930.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3RK3X-ray3.50E26-83[»]
3RL0X-ray3.80g/h/i/j/k/l/m/n26-83[»]
ProteinModelPortaliO14810.
SMRiO14810. Positions 32-72.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116028. 34 interactions.
DIPiDIP-56109N.
IntActiO14810. 2 interactions.
STRINGi9606.ENSP00000305613.

PTM databases

PhosphoSiteiO14810.

Proteomic databases

MaxQBiO14810.
PaxDbiO14810.
PeptideAtlasiO14810.
PRIDEiO14810.

Protocols and materials databases

DNASUi10815.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000304062; ENSP00000305613; ENSG00000168993.
GeneIDi10815.
KEGGihsa:10815.
UCSCiuc003gbi.3. human.

Organism-specific databases

CTDi10815.
GeneCardsiGC04M000778.
HGNCiHGNC:2309. CPLX1.
MIMi605032. gene.
neXtProtiNX_O14810.
PharmGKBiPA26826.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG85303.
GeneTreeiENSGT00490000043416.
HOGENOMiHOG000017716.
HOVERGENiHBG062792.
InParanoidiO14810.
KOiK15294.
OMAiTVMKFIP.
OrthoDBiEOG71G9XG.
PhylomeDBiO14810.
TreeFamiTF315172.

Enzyme and pathway databases

ReactomeiREACT_12591. Glutamate Neurotransmitter Release Cycle.
REACT_15293. Dopamine Neurotransmitter Release Cycle.
REACT_15309. Acetylcholine Neurotransmitter Release Cycle.
REACT_15418. Norepinephrine Neurotransmitter Release Cycle.
REACT_15425. Serotonin Neurotransmitter Release Cycle.
REACT_23947. GABA synthesis, release, reuptake and degradation.

Miscellaneous databases

ChiTaRSiCPLX1. human.
GeneWikiiCPLX1.
GenomeRNAii10815.
NextBioi41085.
PROiO14810.
SOURCEiSearch...

Gene expression databases

BgeeiO14810.
CleanExiHS_CPLX1.
ExpressionAtlasiO14810. baseline and differential.
GenevestigatoriO14810.

Family and domain databases

InterProiIPR008849. Synaphin.
[Graphical view]
PANTHERiPTHR16705. PTHR16705. 1 hit.
PfamiPF05835. Synaphin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complexins: cytosolic proteins that regulate SNAP receptor function."
    McMahon H.T., Missler M., Li C., Suedhof T.C.
    Cell 83:111-119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Fetal brain.
  3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Full-length transcriptome analysis of human retina-derived cell lines ARPE-19 and Y79 using the vector-capping method."
    Oshikawa M., Tsutsui C., Ikegami T., Fuchida Y., Matsubara M., Toyama S., Usami R., Ohtoko K., Kato S.
    Invest. Ophthalmol. Vis. Sci. 52:6662-6670(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Cerebellum.
  6. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  9. "Preferential involvement of excitatory neurons in medial temporal lobe in schizophrenia."
    Harrison P.J., Eastwood S.L.
    Lancet 352:1669-1673(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  10. "Cerebellar synaptic protein expression in schizophrenia."
    Eastwood S.L., Cotter D., Harrison P.J.
    Neuroscience 105:219-229(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  11. "Proteome analysis of human substantia nigra in Parkinson's disease."
    Basso M., Giraudo S., Corpillo D., Bergamasco B., Lopiano L., Fasano M.
    Proteomics 4:3943-3952(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.
  12. Cited for: X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS) OF 28-83 ALONE AND IN COMPLEX WITH SNAP25; VAMP2 AND RAT STX1A, SUBUNIT, FUNCTION.

Entry informationi

Entry nameiCPLX1_HUMAN
AccessioniPrimary (citable) accession number: O14810
Secondary accession number(s): A6NI80
, B2R4R5, D3DVN3, F1T0G1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: January 7, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.