Reviewed,
UniProtKB/Swiss-Prot O14802 (RPC1_HUMAN)
Last modified
July 7, 2009.
Version 79.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: DNA-directed RNA polymerase III subunit RPC1 Short name=RNA polymerase III subunit C1 EC=2.7.7.6 Alternative name(s): DNA-directed RNA polymerase III subunit A DNA-directed RNA polymerase III largest subunit RPC155 RNA polymerase III subunit C160 | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) [Complete proteome] | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 1390 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition By similarity. |
| Catalytic activity | Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). |
| Subunit structure | Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits By similarity. |
| Subcellular location | |
| Sequence similarities | Belongs to the RNA polymerase beta' chain family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Transcription |
| Cellular component | DNA-directed RNA polymerase Nucleus |
| Coding sequence diversity | Polymorphism |
| Domain | Zinc-finger |
| Ligand | Magnesium Metal-binding Zinc |
| Molecular function | Nucleotidyltransferase Transferase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | transcription, DNA-dependent Ref.1 Non-traceable author statement. Source: UniProtKB |
| Cellular component | DNA-directed RNA polymerase III complex Non-traceable author statement. Source: UniProtKB |
| Molecular function | DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Ref.1Non-traceable author statement. Source: UniProtKB magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1390 | 1390 | DNA-directed RNA polymerase III subunit RPC1 | PRO_0000073947 | |||||
Regions | |||||||||
| Zinc finger | 69 – 82 | 14 | C3H1-type Potential | ||||||
| Region | 844 – 856 | 13 | Bridging helix By similarity | ||||||
Sites | |||||||||
| Metal binding | 69 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 72 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 79 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 82 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 109 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 112 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 156 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 499 | 1 | Magnesium; catalytic By similarity | ||||||
| Metal binding | 501 | 1 | Magnesium; catalytic By similarity | ||||||
| Metal binding | 503 | 1 | Magnesium; catalytic By similarity | ||||||
Natural variations | |||||||||
| Natural variant | 582 | 1 | R → L: dbSNP rs34588967. | VAR_051873 | |||||
| Natural variant | 713 | 1 | K → N: dbSNP rs35354908. | VAR_051874 | |||||
Experimental info | |||||||||
| Sequence conflict | 15 | 1 | I → T in AAB86536. Ref.1 | ||||||
| Sequence conflict | 262 | 1 | I → F in AAB86536. Ref.1 | ||||||
| Sequence conflict | 283 | 1 | L → P in AAM12029. Ref.5 | ||||||
| Sequence conflict | 1056 | 1 | A → G in AAB86536. Ref.1 | ||||||
| Sequence conflict | 1275 | 1 | M → MV in AAB86536. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The largest subunit of human RNA polymerase III is closely related to the largest subunit of yeast and trypanosome RNA polymerase III." Sepehri S., Hernandez N. Genome Res. 7:1006-1019(1997) [PubMed: 9331371] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. Rogers J.Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus. |
| [5] | "Identification of an immunodominant epitope on RNA polymerase III recognized by systemic sclerosis sera: application to enzyme-linked immunosorbent assay." Kuwana M., Kimura K., Kawakami Y. Arthritis Rheum. 46:2742-2747(2002) [PubMed: 12384934] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-374. |
| [6] | "Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits." Hu P., Wu S., Sun Y., Yuan C.-C., Kobayashi R., Myers M.P., Hernandez N. Mol. Cell. Biol. 22:8044-8055(2002) [PubMed: 12391170] [Abstract] Cited for: IDENTIFICATION IN THE RNA POL III COMPLEX, MASS SPECTROMETRY. |
| [7] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
Cross-references
Sequence databases | |
|---|---|
| AF021351 mRNA. Translation: AAB86536.1. AL512628 Genomic DNA. Translation: CAI16466.1. CH471083 Genomic DNA. Translation: EAW54617.1. BC041089 mRNA. Translation: AAH41089.1. AY091459 mRNA. Translation: AAM12029.1. | |
| IPI | IPI00024163. |
| RefSeq | NP_008986.2. |
| UniGene | Hs.436896 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1I6H based on UniProtKB P04050. |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | O14802. |
Proteomic databases | |
| PRIDE | O14802. |
Genome annotation databases | |
| Ensembl | ENSG00000148606. Homo sapiens. [Contig view] |
| GeneID | 11128. |
| KEGG | hsa:11128. |
Organism-specific databases | |
| GeneCards | GC10M079405. |
| H-InvDB | HIX0008960. |
| HGNC | HGNC:30074. POLR3A. |
| PharmGKB | PA134900426. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | O14802. |
| HOVERGEN | O14802. |
| OMA | O14802. CCQDSFL. |
Enzyme and pathway databases | |
| BRENDA | 2.7.7.6. 247. |
| Reactome | REACT_1788. Transcription. |
Gene expression databases | |
| ArrayExpress | O14802. |
| Bgee | O14802. |
| CleanEx | HS_POLR3A. |
| GermOnline | ENSG00000148606. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view] |
| Gene3D | G3DSA:2.40.40.30. RNA_pol_A. 1 hit. G3DSA:3.90.1120.10. RNA_pol_Rpb1_1. 1 hit. |
| Pfam | PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view] |
| SMART | SM00663. RPOLA_N. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | RPC1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: O14802 Secondary accession number(s): Q8IW34, Q8TCW5 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 10 Human chromosome 10: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| SIMILARITY comments Index of protein domains and families |

Clusters with


