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Protein

Protein unc-13 homolog B

Gene

UNC13B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-depending refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic but not inhibitory/GABA-mediated synapses (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri477 – 527Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST51

GO - Molecular functioni

  • calmodulin binding Source: ParkinsonsUK-UCL
  • diacylglycerol binding Source: ParkinsonsUK-UCL
  • GTP-dependent protein binding Source: ParkinsonsUK-UCL
  • metal ion binding Source: UniProtKB-KW
  • Rab GTPase binding Source: ParkinsonsUK-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Exocytosis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:G66-33929-MONOMER.
ReactomeiR-HSA-181429. Serotonin Neurotransmitter Release Cycle.
R-HSA-181430. Norepinephrine Neurotransmitter Release Cycle.
R-HSA-210500. Glutamate Neurotransmitter Release Cycle.
R-HSA-212676. Dopamine Neurotransmitter Release Cycle.
R-HSA-264642. Acetylcholine Neurotransmitter Release Cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein unc-13 homolog B
Alternative name(s):
Munc13-2
Short name:
munc13
Gene namesi
Name:UNC13B
Synonyms:UNC13
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:12566. UNC13B.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytosol Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • membrane Source: ParkinsonsUK-UCL
  • neuromuscular junction Source: Ensembl
  • plasma membrane Source: ParkinsonsUK-UCL
  • presynaptic active zone Source: ParkinsonsUK-UCL
  • terminal bouton Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane, Synapse

Pathology & Biotechi

Organism-specific databases

DisGeNETi10497.
OpenTargetsiENSG00000198722.
PharmGKBiPA37203.

Polymorphism and mutation databases

BioMutaiUNC13B.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001885751 – 1591Protein unc-13 homolog BAdd BLAST1591

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16PhosphoserineCombined sources1
Modified residuei176PhosphoserineCombined sources1
Modified residuei295PhosphoserineCombined sources1
Modified residuei367PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO14795.
PaxDbiO14795.
PeptideAtlasiO14795.
PRIDEiO14795.

PTM databases

iPTMnetiO14795.
PhosphoSitePlusiO14795.

Expressioni

Tissue specificityi

Expressed in kidney cortical epithelial cells and brain.1 Publication

Gene expression databases

BgeeiENSG00000198722.
CleanExiHS_UNC13B.
ExpressionAtlasiO14795. baseline and differential.
GenevisibleiO14795. HS.

Organism-specific databases

HPAiHPA024493.
HPA062300.

Interactioni

Subunit structurei

Interacts with RIMS1.By similarity

GO - Molecular functioni

  • calmodulin binding Source: ParkinsonsUK-UCL
  • GTP-dependent protein binding Source: ParkinsonsUK-UCL
  • Rab GTPase binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi115759. 16 interactors.
IntActiO14795. 3 interactors.
MINTiMINT-1395851.
STRINGi9606.ENSP00000367756.

Structurei

3D structure databases

ProteinModelPortaliO14795.
SMRiO14795.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 79C2 1PROSITE-ProRule annotationAdd BLAST79
Domaini587 – 693C2 2PROSITE-ProRule annotationAdd BLAST107
Domaini1013 – 1156MHD1PROSITE-ProRule annotationAdd BLAST144
Domaini1263 – 1405MHD2PROSITE-ProRule annotationAdd BLAST143
Domaini1425 – 1530C2 3PROSITE-ProRule annotationAdd BLAST106

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1172 – 1202Sequence analysisAdd BLAST31

Domaini

The C2 domains are not involved in calcium-dependent phospholipid binding.By similarity

Sequence similaritiesi

Belongs to the unc-13 family.Curated
Contains 3 C2 domains.PROSITE-ProRule annotation
Contains 1 MHD1 (MUNC13 homology domain 1) domain.PROSITE-ProRule annotation
Contains 1 MHD2 (MUNC13 homology domain 2) domain.PROSITE-ProRule annotation
Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri477 – 527Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1011. Eukaryota.
ENOG410XS5D. LUCA.
GeneTreeiENSGT00850000132267.
HOVERGENiHBG057340.
InParanoidiO14795.
KOiK15293.
PhylomeDBiO14795.
TreeFamiTF312844.

Family and domain databases

CDDicd00029. C1. 1 hit.
Gene3Di2.60.40.150. 3 hits.
InterProiIPR000008. C2_dom.
IPR010439. CAPS_dom.
IPR014770. Munc13_1.
IPR014772. Munc13_dom-2.
IPR019558. Munc13_subgr_dom-2.
IPR002219. PE/DAG-bd.
IPR027080. Unc-13.
[Graphical view]
PANTHERiPTHR10480. PTHR10480. 1 hit.
PfamiPF00130. C1_1. 1 hit.
PF00168. C2. 3 hits.
PF06292. DUF1041. 1 hit.
PF10540. Membr_traf_MHD. 1 hit.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00239. C2. 3 hits.
SM01145. DUF1041. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 3 hits.
PROSITEiPS50004. C2. 2 hits.
PS51258. MHD1. 1 hit.
PS51259. MHD2. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O14795-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLLCVRVKR AKFQGSPDKF NTYVTLKVQN VKSTTVAVRG DQPSWEQDFM
60 70 80 90 100
FEISRLDLGL SVEVWNKGLI WDTMVGTVWI ALKTIRQSDE EGPGEWSTLE
110 120 130 140 150
AETLMKDDEI CGTRNPTPHK ILLDTRFELP FDIPEEEARY WTYKWEQINA
160 170 180 190 200
LGADNEYSSQ EESQRKPLPT AAAQCSFEDP DSAVDDRDSD YRSETSNSFP
210 220 230 240 250
PPYHTASQPN ASVHQFPVPV RSPQQLLLQG SSRDSCNDSM QSYDLDYPER
260 270 280 290 300
RAISPTSSSR YGSSCNVSQG SSQLSELDQY HEQDDDHRET DSIHSCHSSH
310 320 330 340 350
SLSRDGQAGF GEQEKPLEVT GQAEKEAACE PKEMKEDATT HPPPDLVLQK
360 370 380 390 400
DHFLGPQESF PEENASSPFT QARAHWIRAV TKVRLQLQEI PDDGDPSLPQ
410 420 430 440 450
WLPEGPAGGL YGIDSMPDLR RKKPLPLVSD LSLVQSRKAG ITSAMATRTS
460 470 480 490 500
LKDEELKSHV YKKTLQALIY PISCTTPHNF EVWTATTPTY CYECEGLLWG
510 520 530 540 550
IARQGMRCSE CGVKCHEKCQ DLLNADCLQR AAEKSCKHGA EDRTQNIIMA
560 570 580 590 600
MKDRMKIRER NKPEIFEVIR DVFTVNKAAH VQQMKTVKQS VLDGTSKWSA
610 620 630 640 650
KITITVVCAQ GLQAKDKTGS SDPYVTVQVS KTKKRTKTIF GNLNPVWEEK
660 670 680 690 700
FHFECHNSSD RIKVRVWDED DDIKSRVKQR LKRESDDFLG QTIIEVRTLS
710 720 730 740 750
GEMDVWYNLE KRTDKSAVSG AIRLQISVEI KGEEKVAPYH VQYTCLHENL
760 770 780 790 800
FHYLTDIQGS GGVRIPEARG DDAWKVYFDE TAQEIVDEFA MRYGIESIYQ
810 820 830 840 850
AMTHFACLSS KYMCPGVPAV MSTLLANINA YYAHTTASTN VSASDRFAAS
860 870 880 890 900
NFGKERFVKL LDQLHNSLRI DLSTYRNNFP AGSPERLQDL KSTVDLLTSI
910 920 930 940 950
TFFRMKVQEL QSPPRASQVV KDCVKACLNS TYEYIFNNCH DLYSRQYQLK
960 970 980 990 1000
QELPPEEQGP SIRNLDFWPK LITLIVSIIE EDKNSYTPVL NQFPQELNVG
1010 1020 1030 1040 1050
KVSAEVMWHL FAQDMKYALE EHEKDHLCKS ADYMNLHFKV KWLHNEYVRD
1060 1070 1080 1090 1100
LPVLQGQVPE YPAWFEQFVL QWLDENEDVS LEFLRGALER DKKDGFQQTS
1110 1120 1130 1140 1150
EHALFSCSVV DVFTQLNQSF EIIRKLECPD PSILAHYMRR FAKTIGKVLM
1160 1170 1180 1190 1200
QYADILSKDF PAYCTKEKLP CILMNNVQQL RVQLEKMFEA MGGKELDLEA
1210 1220 1230 1240 1250
ADSLKELQVK LNTVLDELSM VFGNSFQVRI DECVRQMADI LGQVRGTGNA
1260 1270 1280 1290 1300
SPDARASAAQ DADSVLRPLM DFLDGNLTLF ATVCEKTVLK RVLKELWRVV
1310 1320 1330 1340 1350
MNTMERMIVL PPLTDQTGTQ LIFTAAKELS HLSKLKDHMV REETRNLTPK
1360 1370 1380 1390 1400
QCAVLDLALD TIKQYFHAGG NGLKKTFLEK SPDLQSLRYA LSLYTQTTDT
1410 1420 1430 1440 1450
LIKTFVRSQT TQGSGVDDPV GEVSIQVDLF THPGTGEHKV TVKVVAANDL
1460 1470 1480 1490 1500
KWQTAGMFRP FVEVTMVGPH QSDKKRKFTT KSKSNNWAPK YNETFHFLLG
1510 1520 1530 1540 1550
NEEGPESYEL QICVKDYCFA REDRVLGLAV MPLRDVTAKG SCACWCPLGR
1560 1570 1580 1590
KIHMDETGLT ILRILSQRSN DEVAREFVKL KSESRSTEEG S
Length:1,591
Mass (Da):180,679
Last modified:November 13, 2007 - v2
Checksum:iFBE8D85C55A8D780
GO
Isoform 2 (identifier: O14795-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1412-1412: Q → QVHDGKGIRFTANEDIRPEK

Note: No experimental confirmation available.
Show »
Length:1,610
Mass (Da):182,843
Checksum:iE70016365464C437
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1207L → P in AAC19406 (PubMed:9607201).Curated1
Sequence conflicti1214V → I in AAI11782 (PubMed:15489334).Curated1
Sequence conflicti1404T → R in AAC19406 (PubMed:9607201).Curated1
Sequence conflicti1497F → L in AAC19406 (PubMed:9607201).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_036615209P → S in a colorectal cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_037273238D → E.Corresponds to variant rs35199210dbSNPEnsembl.1
Natural variantiVAR_0372741232E → D.Corresponds to variant rs12339582dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0573191412Q → QVHDGKGIRFTANEDIRPEK in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020202 mRNA. Translation: AAC19406.1.
AL353795, AL160274, AL354669 Genomic DNA. Translation: CAH71004.1.
AL354669, AL160274, AL353795 Genomic DNA. Translation: CAH71208.1.
AL160274, AL353795, AL354669 Genomic DNA. Translation: CAH73361.1.
CH471071 Genomic DNA. Translation: EAW58383.1.
BC111781 mRNA. Translation: AAI11782.1.
CCDSiCCDS6579.1. [O14795-1]
CCDS83361.1. [O14795-2]
RefSeqiNP_001317582.1. NM_001330653.1.
NP_006368.3. NM_006377.3. [O14795-1]
UniGeneiHs.493791.

Genome annotation databases

EnsembliENST00000378495; ENSP00000367756; ENSG00000198722. [O14795-1]
ENST00000619578; ENSP00000479261; ENSG00000198722. [O14795-2]
GeneIDi10497.
KEGGihsa:10497.
UCSCiuc003zwq.4. human. [O14795-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020202 mRNA. Translation: AAC19406.1.
AL353795, AL160274, AL354669 Genomic DNA. Translation: CAH71004.1.
AL354669, AL160274, AL353795 Genomic DNA. Translation: CAH71208.1.
AL160274, AL353795, AL354669 Genomic DNA. Translation: CAH73361.1.
CH471071 Genomic DNA. Translation: EAW58383.1.
BC111781 mRNA. Translation: AAI11782.1.
CCDSiCCDS6579.1. [O14795-1]
CCDS83361.1. [O14795-2]
RefSeqiNP_001317582.1. NM_001330653.1.
NP_006368.3. NM_006377.3. [O14795-1]
UniGeneiHs.493791.

3D structure databases

ProteinModelPortaliO14795.
SMRiO14795.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115759. 16 interactors.
IntActiO14795. 3 interactors.
MINTiMINT-1395851.
STRINGi9606.ENSP00000367756.

PTM databases

iPTMnetiO14795.
PhosphoSitePlusiO14795.

Polymorphism and mutation databases

BioMutaiUNC13B.

Proteomic databases

MaxQBiO14795.
PaxDbiO14795.
PeptideAtlasiO14795.
PRIDEiO14795.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000378495; ENSP00000367756; ENSG00000198722. [O14795-1]
ENST00000619578; ENSP00000479261; ENSG00000198722. [O14795-2]
GeneIDi10497.
KEGGihsa:10497.
UCSCiuc003zwq.4. human. [O14795-1]

Organism-specific databases

CTDi10497.
DisGeNETi10497.
GeneCardsiUNC13B.
H-InvDBHIX0034747.
HGNCiHGNC:12566. UNC13B.
HPAiHPA024493.
HPA062300.
MIMi605836. gene.
neXtProtiNX_O14795.
OpenTargetsiENSG00000198722.
PharmGKBiPA37203.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1011. Eukaryota.
ENOG410XS5D. LUCA.
GeneTreeiENSGT00850000132267.
HOVERGENiHBG057340.
InParanoidiO14795.
KOiK15293.
PhylomeDBiO14795.
TreeFamiTF312844.

Enzyme and pathway databases

BioCyciZFISH:G66-33929-MONOMER.
ReactomeiR-HSA-181429. Serotonin Neurotransmitter Release Cycle.
R-HSA-181430. Norepinephrine Neurotransmitter Release Cycle.
R-HSA-210500. Glutamate Neurotransmitter Release Cycle.
R-HSA-212676. Dopamine Neurotransmitter Release Cycle.
R-HSA-264642. Acetylcholine Neurotransmitter Release Cycle.

Miscellaneous databases

ChiTaRSiUNC13B. human.
GeneWikiiUNC13B.
GenomeRNAii10497.
PROiO14795.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000198722.
CleanExiHS_UNC13B.
ExpressionAtlasiO14795. baseline and differential.
GenevisibleiO14795. HS.

Family and domain databases

CDDicd00029. C1. 1 hit.
Gene3Di2.60.40.150. 3 hits.
InterProiIPR000008. C2_dom.
IPR010439. CAPS_dom.
IPR014770. Munc13_1.
IPR014772. Munc13_dom-2.
IPR019558. Munc13_subgr_dom-2.
IPR002219. PE/DAG-bd.
IPR027080. Unc-13.
[Graphical view]
PANTHERiPTHR10480. PTHR10480. 1 hit.
PfamiPF00130. C1_1. 1 hit.
PF00168. C2. 3 hits.
PF06292. DUF1041. 1 hit.
PF10540. Membr_traf_MHD. 1 hit.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00239. C2. 3 hits.
SM01145. DUF1041. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 3 hits.
PROSITEiPS50004. C2. 2 hits.
PS51258. MHD1. 1 hit.
PS51259. MHD2. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUN13B_HUMAN
AccessioniPrimary (citable) accession number: O14795
Secondary accession number(s): Q2NKJ5, Q5VYM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: November 13, 2007
Last modified: November 30, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.