O14795 (UN13B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein unc-13 homolog B

Alternative name(s):
Munc13-2
Short name=munc13

Gene names

Name:	UNC13B
Synonyms:	UNC13

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1591 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-depending refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic but not inhibitory/GABA-mediated synapses By similarity.
Subunit structure	Interacts with RIMS1 By similarity.
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity. Cell membrane By similarity. Cell junction › synapse By similarity. Note: Localized to synapses. Translocated to the plasma membrane in response to phorbol ester binding By similarity. Ref.4
Tissue specificity	Expressed in kidney cortical epithelial cells and brain. Ref.1
Domain	The C2 domains are not involved in calcium-dependent phospholipid binding By similarity.
Sequence similarities	Belongs to the unc-13 family. Contains 3 C2 domains. Contains 1 MHD1 (MUNC13 homology domain 1) domain. Contains 1 MHD2 (MUNC13 homology domain 2) domain. Contains 1 phorbol-ester/DAG-type zinc finger.

Ontologies

Keywords
Biological process	Exocytosis
Cellular component	Cell junction Cell membrane Cytoplasm Membrane Synapse
Coding sequence diversity	Polymorphism
Domain	Coiled coil Repeat Zinc-finger
Ligand	Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	excretion Traceable author statement Ref.1. Source: ProtInc induction of apoptosis Traceable author statement Ref.4. Source: ProtInc innervation Inferred from electronic annotation. Source: Compara intracellular signal transduction Inferred from electronic annotation. Source: InterPro neuromuscular junction development Inferred from electronic annotation. Source: Compara regulation of short-term neuronal synaptic plasticity Inferred from electronic annotation. Source: Compara synaptic transmission, glutamatergic Inferred from electronic annotation. Source: Compara synaptic vesicle docking involved in exocytosis Inferred from electronic annotation. Source: Compara synaptic vesicle priming Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi apparatus Traceable author statement Ref.1. Source: ProtInc cell junction Inferred from electronic annotation. Source: UniProtKB-KW neuromuscular junction Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	diacylglycerol binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW receptor activity Traceable author statement Ref.1. Source: ProtInc signal transducer activity Traceable author statement Ref.4Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1591	1591	Protein unc-13 homolog B		PRO_0000188575
Regions
	Domain	1 – 79	79	C2 1
	Domain	587 – 693	107	C2 2
	Domain	1013 – 1156	144	MHD1
	Domain	1263 – 1405	143	MHD2
	Domain	1425 – 1530	106	C2 3
	Zinc finger	477 – 527	51	Phorbol-ester/DAG-type
	Coiled coil	1172 – 1202	31	Potential
Natural variations
	Natural variant	209	1	P → S in a colorectal cancer sample; somatic mutation. Ref.7		VAR_036615
	Natural variant	238	1	D → E. Corresponds to variant rs35199210 [ dbSNP | Ensembl ].		VAR_037273
	Natural variant	1232	1	E → D. Corresponds to variant rs12339582 [ dbSNP | Ensembl ].		VAR_037274
Experimental info
	Sequence conflict	1207	1	L → P in AAC19406. Ref.1
	Sequence conflict	1404	1	T → R in AAC19406. Ref.1
	Sequence conflict	1497	1	F → L in AAC19406. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O14795 [UniParc]. Last modified November 13, 2007. Version 2. Checksum: FBE8D85C55A8D780	FASTA	1,591	180,679
	10 20 30 40 50 60 MSLLCVRVKR AKFQGSPDKF NTYVTLKVQN VKSTTVAVRG DQPSWEQDFM FEISRLDLGL 70 80 90 100 110 120 SVEVWNKGLI WDTMVGTVWI ALKTIRQSDE EGPGEWSTLE AETLMKDDEI CGTRNPTPHK 130 140 150 160 170 180 ILLDTRFELP FDIPEEEARY WTYKWEQINA LGADNEYSSQ EESQRKPLPT AAAQCSFEDP 190 200 210 220 230 240 DSAVDDRDSD YRSETSNSFP PPYHTASQPN ASVHQFPVPV RSPQQLLLQG SSRDSCNDSM 250 260 270 280 290 300 QSYDLDYPER RAISPTSSSR YGSSCNVSQG SSQLSELDQY HEQDDDHRET DSIHSCHSSH 310 320 330 340 350 360 SLSRDGQAGF GEQEKPLEVT GQAEKEAACE PKEMKEDATT HPPPDLVLQK DHFLGPQESF 370 380 390 400 410 420 PEENASSPFT QARAHWIRAV TKVRLQLQEI PDDGDPSLPQ WLPEGPAGGL YGIDSMPDLR 430 440 450 460 470 480 RKKPLPLVSD LSLVQSRKAG ITSAMATRTS LKDEELKSHV YKKTLQALIY PISCTTPHNF 490 500 510 520 530 540 EVWTATTPTY CYECEGLLWG IARQGMRCSE CGVKCHEKCQ DLLNADCLQR AAEKSCKHGA 550 560 570 580 590 600 EDRTQNIIMA MKDRMKIRER NKPEIFEVIR DVFTVNKAAH VQQMKTVKQS VLDGTSKWSA 610 620 630 640 650 660 KITITVVCAQ GLQAKDKTGS SDPYVTVQVS KTKKRTKTIF GNLNPVWEEK FHFECHNSSD 670 680 690 700 710 720 RIKVRVWDED DDIKSRVKQR LKRESDDFLG QTIIEVRTLS GEMDVWYNLE KRTDKSAVSG 730 740 750 760 770 780 AIRLQISVEI KGEEKVAPYH VQYTCLHENL FHYLTDIQGS GGVRIPEARG DDAWKVYFDE 790 800 810 820 830 840 TAQEIVDEFA MRYGIESIYQ AMTHFACLSS KYMCPGVPAV MSTLLANINA YYAHTTASTN 850 860 870 880 890 900 VSASDRFAAS NFGKERFVKL LDQLHNSLRI DLSTYRNNFP AGSPERLQDL KSTVDLLTSI 910 920 930 940 950 960 TFFRMKVQEL QSPPRASQVV KDCVKACLNS TYEYIFNNCH DLYSRQYQLK QELPPEEQGP 970 980 990 1000 1010 1020 SIRNLDFWPK LITLIVSIIE EDKNSYTPVL NQFPQELNVG KVSAEVMWHL FAQDMKYALE 1030 1040 1050 1060 1070 1080 EHEKDHLCKS ADYMNLHFKV KWLHNEYVRD LPVLQGQVPE YPAWFEQFVL QWLDENEDVS 1090 1100 1110 1120 1130 1140 LEFLRGALER DKKDGFQQTS EHALFSCSVV DVFTQLNQSF EIIRKLECPD PSILAHYMRR 1150 1160 1170 1180 1190 1200 FAKTIGKVLM QYADILSKDF PAYCTKEKLP CILMNNVQQL RVQLEKMFEA MGGKELDLEA 1210 1220 1230 1240 1250 1260 ADSLKELQVK LNTVLDELSM VFGNSFQVRI DECVRQMADI LGQVRGTGNA SPDARASAAQ 1270 1280 1290 1300 1310 1320 DADSVLRPLM DFLDGNLTLF ATVCEKTVLK RVLKELWRVV MNTMERMIVL PPLTDQTGTQ 1330 1340 1350 1360 1370 1380 LIFTAAKELS HLSKLKDHMV REETRNLTPK QCAVLDLALD TIKQYFHAGG NGLKKTFLEK 1390 1400 1410 1420 1430 1440 SPDLQSLRYA LSLYTQTTDT LIKTFVRSQT TQGSGVDDPV GEVSIQVDLF THPGTGEHKV 1450 1460 1470 1480 1490 1500 TVKVVAANDL KWQTAGMFRP FVEVTMVGPH QSDKKRKFTT KSKSNNWAPK YNETFHFLLG 1510 1520 1530 1540 1550 1560 NEEGPESYEL QICVKDYCFA REDRVLGLAV MPLRDVTAKG SCACWCPLGR KIHMDETGLT 1570 1580 1590 ILRILSQRSN DEVAREFVKL KSESRSTEEG S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a novel gene in the human kidney homologous to rat munc13s: its potential role in diabetic nephropathy." Song Y., Ailenberg M., Silverman M. Kidney Int. 53:1689-1695(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Kidney.
[2]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Human munc13 is a diacylglycerol receptor that induces apoptosis and may contribute to renal cell injury in hyperglycemia." Song Y., Ailenberg M., Silverman M. Mol. Biol. Cell 10:1609-1619(1999) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[6]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[7]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] SER-209.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF020202 mRNA. Translation: AAC19406.1. AL353795, AL160274, AL354669 Genomic DNA. Translation: CAH71004.1. AL354669, AL160274, AL353795 Genomic DNA. Translation: CAH71208.1. AL160274, AL353795, AL354669 Genomic DNA. Translation: CAH73361.1. CH471071 Genomic DNA. Translation: EAW58383.1.
IPI	IPI00478689.
RefSeq	NP_006368.3. NM_006377.3.
UniGene	Hs.493791.
3D structure databases
ProteinModelPortal	O14795.
ModBase	Search...
Protein-protein interaction databases
IntAct	O14795. 2 interactions.
MINT	MINT-1395851.
STRING	9606.ENSP00000367756.
PTM databases
PhosphoSite	O14795.
Proteomic databases
PaxDb	O14795.
PRIDE	O14795.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000378495; ENSP00000367756; ENSG00000198722.
GeneID	10497.
KEGG	hsa:10497.
UCSC	uc003zwq.3. human.
Organism-specific databases
CTD	10497.
GeneCards	GC09P035117.
H-InvDB	HIX0034747.
HGNC	HGNC:12566. UNC13B.
HPA	HPA024493.
MIM	605836. gene.
neXtProt	NX_O14795.
PharmGKB	PA37203.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG245727.
HOVERGEN	HBG057340.
KO	K15293.
PhylomeDB	O14795.
Enzyme and pathway databases
Pathway_Interaction_DB	botulinumtoxinpathway. Effects of Botulinum toxin.
Gene expression databases
ArrayExpress	O14795.
Bgee	O14795.
CleanEx	HS_UNC13B.
Genevestigator	O14795.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR020477. C2_dom. IPR018029. C2_membr_targeting. IPR010439. Ca-dep_secretion_activator. IPR014770. Munc13_1. IPR014772. Munc13_dom-2. IPR019558. Munc13_subgr_dom-2. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. IPR027080. Unc-13. [Graphical view]
PANTHER	PTHR10480. PTHR10480. 1 hit.
Pfam	PF00130. C1_1. 1 hit. PF00168. C2. 3 hits. PF06292. DUF1041. 1 hit. PF10540. Membr_traf_MHD. 1 hit. [Graphical view]
PRINTS	PR00360. C2DOMAIN.
SMART	SM00109. C1. 1 hit. SM00239. C2. 3 hits. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 3 hits.
PROSITE	PS50004. C2. 2 hits. PS51258. MHD1. 1 hit. PS51259. MHD2. 1 hit. PS00479. ZF_DAG_PE_1. 1 hit. PS50081. ZF_DAG_PE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	10497.
NextBio	39832.
SOURCE	Search...

Entry information

Entry name

UN13B_HUMAN

Accession

Primary (citable) accession number: O14795
Secondary accession number(s): Q5VYM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	November 13, 2007
Last modified:	May 1, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.