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O14787

- TNPO2_HUMAN

UniProt

O14787 - TNPO2_HUMAN

Protein

Transportin-2

Gene

TNPO2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 3 (24 Nov 2009)
      Previous versions | rss
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    Functioni

    Probably functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus By similarity.By similarity

    GO - Molecular functioni

    1. nuclear localization sequence binding Source: ProtInc
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. intracellular protein transport Source: InterPro

    Keywords - Biological processi

    Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transportin-2
    Alternative name(s):
    Karyopherin beta-2b
    Gene namesi
    Name:TNPO2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:19998. TNPO2.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: ProtInc
    2. nucleus Source: ProtInc

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134921349.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 897897Transportin-2PRO_0000120767Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei862 – 8621N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiO14787.
    PaxDbiO14787.
    PRIDEiO14787.

    PTM databases

    PhosphoSiteiO14787.

    Expressioni

    Gene expression databases

    ArrayExpressiO14787.
    BgeeiO14787.
    CleanExiHS_TNPO2.
    GenevestigatoriO14787.

    Organism-specific databases

    HPAiCAB046446.

    Interactioni

    Protein-protein interaction databases

    BioGridi119024. 10 interactions.
    IntActiO14787. 5 interactions.
    MINTiMINT-2998321.
    STRINGi9606.ENSP00000397379.

    Structurei

    3D structure databases

    ProteinModelPortaliO14787.
    SMRiO14787. Positions 3-896.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini31 – 9969Importin N-terminalAdd
    BLAST
    Repeati121 – 15939HEAT 1Add
    BLAST
    Repeati167 – 20539HEAT 2Add
    BLAST
    Repeati208 – 24639HEAT 3Add
    BLAST
    Repeati249 – 28739HEAT 4Add
    BLAST
    Repeati292 – 33039HEAT 5Add
    BLAST
    Repeati389 – 42739HEAT 6Add
    BLAST
    Repeati430 – 46839HEAT 7Add
    BLAST
    Repeati472 – 51039HEAT 8Add
    BLAST
    Repeati513 – 55139HEAT 9Add
    BLAST
    Repeati556 – 59641HEAT 10Add
    BLAST
    Repeati659 – 69739HEAT 11Add
    BLAST
    Repeati700 – 73839HEAT 12Add
    BLAST
    Repeati741 – 78040HEAT 13Add
    BLAST

    Sequence similaritiesi

    Belongs to the importin beta family.Curated
    Contains 13 HEAT repeats.Curated
    Contains 1 importin N-terminal domain.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5215.
    HOGENOMiHOG000203940.
    HOVERGENiHBG058963.
    InParanoidiO14787.
    OMAiVRRHVCQ.
    OrthoDBiEOG7XM2X0.
    PhylomeDBiO14787.
    TreeFamiTF300825.

    Family and domain databases

    Gene3Di1.25.10.10. 2 hits.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR000357. HEAT.
    IPR001494. Importin-beta_N.
    [Graphical view]
    PfamiPF02985. HEAT. 1 hit.
    PF03810. IBN_N. 1 hit.
    [Graphical view]
    SMARTiSM00913. IBN_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O14787-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDWQPDEQGL QQVLQLLKDS QSPNTATQRI VQDKLKQLNQ FPDFNNYLIF    50
    VLTRLKSEDE PTRSLSGLIL KNNVKAHYQS FPPPVADFIK QECLNNIGDA 100
    SSLIRATIGI LITTIASKGE LQMWPELLPQ LCNLLNSEDY NTCEGAFGAL 150
    QKICEDSSEL LDSDALNRPL NIMIPKFLQF FKHCSPKIRS HAIACVNQFI 200
    MDRAQALMDN IDTFIEHLFA LAVDDDPEVR KNVCRALVML LEVRIDRLIP 250
    HMHSIIQYML QRTQDHDENV ALEACEFWLT LAEQPICKEV LASHLVQLIP 300
    ILVNGMKYSE IDIILLKGDV EEDEAVPDSE QDIKPRFHKS RTVTLPHEAE 350
    RPDGSEDAED DDDDDALSDW NLRKCSAAAL DVLANVFREE LLPHLLPLLK 400
    GLLFHPEWVV KESGILVLGA IAEGCMQGMV PYLPELIPHL IQCLSDKKAL 450
    VRSIACWTLS RYAHWVVSQP PDMHLKPLMT ELLKRILDGN KRVQEAACSA 500
    FATLEEEACT ELVPYLSYIL DTLVFAFGKY QHKNLLILYD AIGTLADSVG 550
    HHLNQPEYIQ KLMPPLIQKW NELKDEDKDL FPLLECLSSV ATALQSGFLP 600
    YCEPVYQRCV TLVQKTLAQA MMYTQHPEQY EAPDKDFMIV ALDLLSGLAE 650
    GLGGHVEQLV ARSNIMTLLF QCMQDSMPEV RQSSFALLGD LTKACFIHVK 700
    PCIAEFMPIL GTNLNPEFIS VCNNATWAIG EICMQMGAEM QPYVQMVLNN 750
    LVEIINRPNT PKTLLENTGR LTSPSAIPAI TIGRLGYVCP QEVAPMLQQF 800
    IRPWCTSLRN IRDNEEKDSA FRGICMMIGV NPGGVVQDFI FFCDAVASWV 850
    SPKDDLRDMF YKILHGFKDQ VGEDNWQQFS EQFPPLLKER LAAFYGV 897
    Length:897
    Mass (Da):101,388
    Last modified:November 24, 2009 - v3
    Checksum:i26FE455583D7D35F
    GO
    Isoform 2 (identifier: O14787-2) [UniParc]FASTAAdd to Basket

    Also known as: beta2b

    The sequence of this isoform differs from the canonical sequence as follows:
         769-778: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:887
    Mass (Da):100,408
    Checksum:iAE64FDCE4DA901DB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti28 – 281Q → H in AAB71349. 1 PublicationCurated
    Sequence conflicti32 – 321Q → R in AAB71349. 1 PublicationCurated
    Sequence conflicti194 – 1941A → G in AAB71349. 1 PublicationCurated
    Sequence conflicti398 – 4014LLKG → YQS in AAB83973. (PubMed:9298975)Curated
    Sequence conflicti492 – 4921R → K in AAB71349. 1 PublicationCurated
    Sequence conflicti499 – 4991S → I in AAB71349. 1 PublicationCurated
    Sequence conflicti507 – 5071E → K in AAB71349. 1 PublicationCurated
    Sequence conflicti608 – 6081R → C in AAB71349. 1 PublicationCurated
    Sequence conflicti645 – 6451L → F in AAB71349. 1 PublicationCurated
    Sequence conflicti646 – 6461S → T in AAB83973. (PubMed:9298975)Curated
    Sequence conflicti656 – 6561V → L in AAB83973. (PubMed:9298975)Curated
    Sequence conflicti687 – 6871L → F in AAB71349. 1 PublicationCurated
    Sequence conflicti691 – 6911L → F in AAB71349. 1 PublicationCurated
    Sequence conflicti696 – 6972FI → SS in AAB71349. 1 PublicationCurated
    Sequence conflicti717 – 7171E → K in AAB83973. (PubMed:9298975)Curated
    Sequence conflicti812 – 8121R → Q in AAB83973. (PubMed:9298975)Curated
    Sequence conflicti841 – 8411F → L in AAB71349. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti370 – 3701W → C Found in a patient with severely delayed development, autism, myopia, strabismus and some dysmorphisms. 1 Publication
    VAR_069373

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei769 – 77810Missing in isoform 2. 2 PublicationsVSP_009657

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF019039 mRNA. Translation: AAB83973.1.
    AF007748 mRNA. Translation: AAB71349.1.
    AC018761 Genomic DNA. No translation available.
    CH471106 Genomic DNA. Translation: EAW84300.1.
    BC072420 mRNA. Translation: AAH72420.1.
    CCDSiCCDS45991.1. [O14787-1]
    CCDS45992.1. [O14787-2]
    RefSeqiNP_001129667.1. NM_001136195.1. [O14787-2]
    NP_001129668.1. NM_001136196.1. [O14787-1]
    NP_038461.2. NM_013433.4. [O14787-2]
    UniGeneiHs.416049.
    Hs.714402.

    Genome annotation databases

    EnsembliENST00000356861; ENSP00000349321; ENSG00000105576. [O14787-2]
    ENST00000425528; ENSP00000407182; ENSG00000105576. [O14787-1]
    ENST00000450764; ENSP00000397379; ENSG00000105576. [O14787-2]
    ENST00000588216; ENSP00000465625; ENSG00000105576. [O14787-2]
    ENST00000592287; ENSP00000468434; ENSG00000105576. [O14787-1]
    GeneIDi30000.
    KEGGihsa:30000.
    UCSCiuc002muo.3. human. [O14787-1]
    uc002muq.3. human. [O14787-2]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF019039 mRNA. Translation: AAB83973.1 .
    AF007748 mRNA. Translation: AAB71349.1 .
    AC018761 Genomic DNA. No translation available.
    CH471106 Genomic DNA. Translation: EAW84300.1 .
    BC072420 mRNA. Translation: AAH72420.1 .
    CCDSi CCDS45991.1. [O14787-1 ]
    CCDS45992.1. [O14787-2 ]
    RefSeqi NP_001129667.1. NM_001136195.1. [O14787-2 ]
    NP_001129668.1. NM_001136196.1. [O14787-1 ]
    NP_038461.2. NM_013433.4. [O14787-2 ]
    UniGenei Hs.416049.
    Hs.714402.

    3D structure databases

    ProteinModelPortali O14787.
    SMRi O14787. Positions 3-896.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119024. 10 interactions.
    IntActi O14787. 5 interactions.
    MINTi MINT-2998321.
    STRINGi 9606.ENSP00000397379.

    PTM databases

    PhosphoSitei O14787.

    Proteomic databases

    MaxQBi O14787.
    PaxDbi O14787.
    PRIDEi O14787.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000356861 ; ENSP00000349321 ; ENSG00000105576 . [O14787-2 ]
    ENST00000425528 ; ENSP00000407182 ; ENSG00000105576 . [O14787-1 ]
    ENST00000450764 ; ENSP00000397379 ; ENSG00000105576 . [O14787-2 ]
    ENST00000588216 ; ENSP00000465625 ; ENSG00000105576 . [O14787-2 ]
    ENST00000592287 ; ENSP00000468434 ; ENSG00000105576 . [O14787-1 ]
    GeneIDi 30000.
    KEGGi hsa:30000.
    UCSCi uc002muo.3. human. [O14787-1 ]
    uc002muq.3. human. [O14787-2 ]

    Organism-specific databases

    CTDi 30000.
    GeneCardsi GC19M012810.
    HGNCi HGNC:19998. TNPO2.
    HPAi CAB046446.
    MIMi 603002. gene.
    neXtProti NX_O14787.
    PharmGKBi PA134921349.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5215.
    HOGENOMi HOG000203940.
    HOVERGENi HBG058963.
    InParanoidi O14787.
    OMAi VRRHVCQ.
    OrthoDBi EOG7XM2X0.
    PhylomeDBi O14787.
    TreeFami TF300825.

    Miscellaneous databases

    ChiTaRSi TNPO2. human.
    GeneWikii TNPO2.
    GenomeRNAii 30000.
    NextBioi 52812.
    PROi O14787.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O14787.
    Bgeei O14787.
    CleanExi HS_TNPO2.
    Genevestigatori O14787.

    Family and domain databases

    Gene3Di 1.25.10.10. 2 hits.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR000357. HEAT.
    IPR001494. Importin-beta_N.
    [Graphical view ]
    Pfami PF02985. HEAT. 1 hit.
    PF03810. IBN_N. 1 hit.
    [Graphical view ]
    SMARTi SM00913. IBN_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins."
      Siomi M.C., Eder P.S., Kataoka N., Wan L., Liu Q., Dreyfuss G.
      J. Cell Biol. 138:1181-1192(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Human karyopherin beta2b: an homolog of human karyopherin beta2."
      Bonifaci N., Radu A., Blobel G.
      Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    3. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: PNS.
    6. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-862, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: VARIANT CYS-370.

    Entry informationi

    Entry nameiTNPO2_HUMAN
    AccessioniPrimary (citable) accession number: O14787
    Secondary accession number(s): O14655, Q6IN77
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2004
    Last sequence update: November 24, 2009
    Last modified: October 1, 2014
    This is version 113 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3