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Protein

Transportin-2

Gene

TNPO2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity).By similarity

GO - Molecular functioni

  1. nuclear localization sequence binding Source: ProtInc

GO - Biological processi

  1. intracellular protein transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Transportin-2
Alternative name(s):
Karyopherin beta-2b
Gene namesi
Name:TNPO2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:19998. TNPO2.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: ProtInc
  2. nucleus Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134921349.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 897897Transportin-2PRO_0000120767Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei862 – 8621N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO14787.
PaxDbiO14787.
PRIDEiO14787.

PTM databases

PhosphoSiteiO14787.

Expressioni

Gene expression databases

BgeeiO14787.
CleanExiHS_TNPO2.
ExpressionAtlasiO14787. baseline and differential.
GenevestigatoriO14787.

Organism-specific databases

HPAiCAB046446.

Interactioni

Protein-protein interaction databases

BioGridi119024. 36 interactions.
IntActiO14787. 5 interactions.
MINTiMINT-2998321.
STRINGi9606.ENSP00000397379.

Structurei

3D structure databases

ProteinModelPortaliO14787.
SMRiO14787. Positions 3-896.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati9 – 3628HEAT 1By similarityAdd
BLAST
Domaini31 – 9969Importin N-terminalAdd
BLAST
Repeati41 – 7939HEAT 2By similarityAdd
BLAST
Repeati88 – 12134HEAT 3By similarityAdd
BLAST
Repeati127 – 16438HEAT 4By similarityAdd
BLAST
Repeati171 – 20131HEAT 5By similarityAdd
BLAST
Repeati214 – 24128HEAT 6By similarityAdd
BLAST
Repeati253 – 28028HEAT 7By similarityAdd
BLAST
Repeati296 – 38691HEAT 8By similarityAdd
BLAST
Repeati394 – 42229HEAT 9By similarityAdd
BLAST
Repeati434 – 46128HEAT 10By similarityAdd
BLAST
Repeati475 – 50834HEAT 11By similarityAdd
BLAST
Repeati516 – 54934HEAT 12By similarityAdd
BLAST
Repeati557 – 59539HEAT 13By similarityAdd
BLAST
Repeati603 – 65452HEAT 14By similarityAdd
BLAST
Repeati665 – 69632HEAT 15By similarityAdd
BLAST
Repeati704 – 73734HEAT 16By similarityAdd
BLAST
Repeati745 – 79046HEAT 17By similarityAdd
BLAST
Repeati798 – 83134HEAT 18By similarityAdd
BLAST
Repeati840 – 87132HEAT 19By similarityAdd
BLAST
Repeati874 – 89421HEAT 20By similarityAdd
BLAST

Sequence similaritiesi

Contains 20 HEAT repeats.By similarity
Contains 1 importin N-terminal domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5215.
GeneTreeiENSGT00550000074720.
HOGENOMiHOG000203940.
HOVERGENiHBG058963.
InParanoidiO14787.
KOiK18727.
OMAiVEMQPYI.
OrthoDBiEOG7XM2X0.
PhylomeDBiO14787.
TreeFamiTF300825.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF02985. HEAT. 1 hit.
PF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O14787-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDWQPDEQGL QQVLQLLKDS QSPNTATQRI VQDKLKQLNQ FPDFNNYLIF
60 70 80 90 100
VLTRLKSEDE PTRSLSGLIL KNNVKAHYQS FPPPVADFIK QECLNNIGDA
110 120 130 140 150
SSLIRATIGI LITTIASKGE LQMWPELLPQ LCNLLNSEDY NTCEGAFGAL
160 170 180 190 200
QKICEDSSEL LDSDALNRPL NIMIPKFLQF FKHCSPKIRS HAIACVNQFI
210 220 230 240 250
MDRAQALMDN IDTFIEHLFA LAVDDDPEVR KNVCRALVML LEVRIDRLIP
260 270 280 290 300
HMHSIIQYML QRTQDHDENV ALEACEFWLT LAEQPICKEV LASHLVQLIP
310 320 330 340 350
ILVNGMKYSE IDIILLKGDV EEDEAVPDSE QDIKPRFHKS RTVTLPHEAE
360 370 380 390 400
RPDGSEDAED DDDDDALSDW NLRKCSAAAL DVLANVFREE LLPHLLPLLK
410 420 430 440 450
GLLFHPEWVV KESGILVLGA IAEGCMQGMV PYLPELIPHL IQCLSDKKAL
460 470 480 490 500
VRSIACWTLS RYAHWVVSQP PDMHLKPLMT ELLKRILDGN KRVQEAACSA
510 520 530 540 550
FATLEEEACT ELVPYLSYIL DTLVFAFGKY QHKNLLILYD AIGTLADSVG
560 570 580 590 600
HHLNQPEYIQ KLMPPLIQKW NELKDEDKDL FPLLECLSSV ATALQSGFLP
610 620 630 640 650
YCEPVYQRCV TLVQKTLAQA MMYTQHPEQY EAPDKDFMIV ALDLLSGLAE
660 670 680 690 700
GLGGHVEQLV ARSNIMTLLF QCMQDSMPEV RQSSFALLGD LTKACFIHVK
710 720 730 740 750
PCIAEFMPIL GTNLNPEFIS VCNNATWAIG EICMQMGAEM QPYVQMVLNN
760 770 780 790 800
LVEIINRPNT PKTLLENTGR LTSPSAIPAI TIGRLGYVCP QEVAPMLQQF
810 820 830 840 850
IRPWCTSLRN IRDNEEKDSA FRGICMMIGV NPGGVVQDFI FFCDAVASWV
860 870 880 890
SPKDDLRDMF YKILHGFKDQ VGEDNWQQFS EQFPPLLKER LAAFYGV
Length:897
Mass (Da):101,388
Last modified:November 24, 2009 - v3
Checksum:i26FE455583D7D35F
GO
Isoform 2 (identifier: O14787-2) [UniParc]FASTAAdd to Basket

Also known as: beta2b

The sequence of this isoform differs from the canonical sequence as follows:
     769-778: Missing.

Note: No experimental confirmation available.

Show »
Length:887
Mass (Da):100,408
Checksum:iAE64FDCE4DA901DB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281Q → H in AAB71349. 1 PublicationCurated
Sequence conflicti32 – 321Q → R in AAB71349. 1 PublicationCurated
Sequence conflicti194 – 1941A → G in AAB71349. 1 PublicationCurated
Sequence conflicti398 – 4014LLKG → YQS in AAB83973. (PubMed:9298975)Curated
Sequence conflicti492 – 4921R → K in AAB71349. 1 PublicationCurated
Sequence conflicti499 – 4991S → I in AAB71349. 1 PublicationCurated
Sequence conflicti507 – 5071E → K in AAB71349. 1 PublicationCurated
Sequence conflicti608 – 6081R → C in AAB71349. 1 PublicationCurated
Sequence conflicti645 – 6451L → F in AAB71349. 1 PublicationCurated
Sequence conflicti646 – 6461S → T in AAB83973. (PubMed:9298975)Curated
Sequence conflicti656 – 6561V → L in AAB83973. (PubMed:9298975)Curated
Sequence conflicti687 – 6871L → F in AAB71349. 1 PublicationCurated
Sequence conflicti691 – 6911L → F in AAB71349. 1 PublicationCurated
Sequence conflicti696 – 6972FI → SS in AAB71349. 1 PublicationCurated
Sequence conflicti717 – 7171E → K in AAB83973. (PubMed:9298975)Curated
Sequence conflicti812 – 8121R → Q in AAB83973. (PubMed:9298975)Curated
Sequence conflicti841 – 8411F → L in AAB71349. 1 PublicationCurated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti370 – 3701W → C Found in a patient with severely delayed development, autism, myopia, strabismus and some dysmorphisms. 1 Publication
VAR_069373

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei769 – 77810Missing in isoform 2. 2 PublicationsVSP_009657

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019039 mRNA. Translation: AAB83973.1.
AF007748 mRNA. Translation: AAB71349.1.
AC018761 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84300.1.
BC072420 mRNA. Translation: AAH72420.1.
CCDSiCCDS45991.1. [O14787-1]
CCDS45992.1. [O14787-2]
RefSeqiNP_001129667.1. NM_001136195.1. [O14787-2]
NP_001129668.1. NM_001136196.1. [O14787-1]
NP_038461.2. NM_013433.4. [O14787-2]
UniGeneiHs.416049.
Hs.714402.

Genome annotation databases

EnsembliENST00000356861; ENSP00000349321; ENSG00000105576. [O14787-2]
ENST00000425528; ENSP00000407182; ENSG00000105576. [O14787-1]
ENST00000450764; ENSP00000397379; ENSG00000105576. [O14787-2]
ENST00000588216; ENSP00000465625; ENSG00000105576. [O14787-2]
ENST00000592287; ENSP00000468434; ENSG00000105576. [O14787-1]
GeneIDi30000.
KEGGihsa:30000.
UCSCiuc002muo.3. human. [O14787-1]
uc002muq.3. human. [O14787-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019039 mRNA. Translation: AAB83973.1.
AF007748 mRNA. Translation: AAB71349.1.
AC018761 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84300.1.
BC072420 mRNA. Translation: AAH72420.1.
CCDSiCCDS45991.1. [O14787-1]
CCDS45992.1. [O14787-2]
RefSeqiNP_001129667.1. NM_001136195.1. [O14787-2]
NP_001129668.1. NM_001136196.1. [O14787-1]
NP_038461.2. NM_013433.4. [O14787-2]
UniGeneiHs.416049.
Hs.714402.

3D structure databases

ProteinModelPortaliO14787.
SMRiO14787. Positions 3-896.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119024. 36 interactions.
IntActiO14787. 5 interactions.
MINTiMINT-2998321.
STRINGi9606.ENSP00000397379.

PTM databases

PhosphoSiteiO14787.

Proteomic databases

MaxQBiO14787.
PaxDbiO14787.
PRIDEiO14787.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000356861; ENSP00000349321; ENSG00000105576. [O14787-2]
ENST00000425528; ENSP00000407182; ENSG00000105576. [O14787-1]
ENST00000450764; ENSP00000397379; ENSG00000105576. [O14787-2]
ENST00000588216; ENSP00000465625; ENSG00000105576. [O14787-2]
ENST00000592287; ENSP00000468434; ENSG00000105576. [O14787-1]
GeneIDi30000.
KEGGihsa:30000.
UCSCiuc002muo.3. human. [O14787-1]
uc002muq.3. human. [O14787-2]

Organism-specific databases

CTDi30000.
GeneCardsiGC19M012810.
HGNCiHGNC:19998. TNPO2.
HPAiCAB046446.
MIMi603002. gene.
neXtProtiNX_O14787.
PharmGKBiPA134921349.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5215.
GeneTreeiENSGT00550000074720.
HOGENOMiHOG000203940.
HOVERGENiHBG058963.
InParanoidiO14787.
KOiK18727.
OMAiVEMQPYI.
OrthoDBiEOG7XM2X0.
PhylomeDBiO14787.
TreeFamiTF300825.

Miscellaneous databases

ChiTaRSiTNPO2. human.
GeneWikiiTNPO2.
GenomeRNAii30000.
NextBioi52812.
PROiO14787.
SOURCEiSearch...

Gene expression databases

BgeeiO14787.
CleanExiHS_TNPO2.
ExpressionAtlasiO14787. baseline and differential.
GenevestigatoriO14787.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF02985. HEAT. 1 hit.
PF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins."
    Siomi M.C., Eder P.S., Kataoka N., Wan L., Liu Q., Dreyfuss G.
    J. Cell Biol. 138:1181-1192(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Human karyopherin beta2b: an homolog of human karyopherin beta2."
    Bonifaci N., Radu A., Blobel G.
    Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  3. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: PNS.
  6. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-862, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: VARIANT CYS-370.

Entry informationi

Entry nameiTNPO2_HUMAN
AccessioniPrimary (citable) accession number: O14787
Secondary accession number(s): O14655, Q6IN77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: November 24, 2009
Last modified: February 4, 2015
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.