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O14787 (TNPO2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transportin-2
Alternative name(s):
Karyopherin beta-2b
Gene names
Name:TNPO2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length897 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probably functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Sequence similarities

Belongs to the importin beta family.

Contains 13 HEAT repeats.

Contains 1 importin N-terminal domain.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processintracellular protein transport

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Traceable author statement Ref.1. Source: ProtInc

nucleus

Traceable author statement Ref.1. Source: ProtInc

   Molecular_functionnuclear localization sequence binding

Traceable author statement Ref.1. Source: ProtInc

protein binding

Inferred from physical interaction PubMed 12896982. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O14787-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O14787-2)

Also known as: beta2b;

The sequence of this isoform differs from the canonical sequence as follows:
     769-778: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 897897Transportin-2
PRO_0000120767

Regions

Domain31 – 9969Importin N-terminal
Repeat121 – 15939HEAT 1
Repeat167 – 20539HEAT 2
Repeat208 – 24639HEAT 3
Repeat249 – 28739HEAT 4
Repeat292 – 33039HEAT 5
Repeat389 – 42739HEAT 6
Repeat430 – 46839HEAT 7
Repeat472 – 51039HEAT 8
Repeat513 – 55139HEAT 9
Repeat556 – 59641HEAT 10
Repeat659 – 69739HEAT 11
Repeat700 – 73839HEAT 12
Repeat741 – 78040HEAT 13

Amino acid modifications

Modified residue8621N6-acetyllysine Ref.6

Natural variations

Alternative sequence769 – 77810Missing in isoform 2.
VSP_009657
Natural variant3701W → C Found in a patient with severely delayed development, autism, myopia, strabismus and some dysmorphisms. Ref.8
VAR_069373

Experimental info

Sequence conflict281Q → H in AAB71349. Ref.2
Sequence conflict321Q → R in AAB71349. Ref.2
Sequence conflict1941A → G in AAB71349. Ref.2
Sequence conflict398 – 4014LLKG → YQS in AAB83973. Ref.1
Sequence conflict4921R → K in AAB71349. Ref.2
Sequence conflict4991S → I in AAB71349. Ref.2
Sequence conflict5071E → K in AAB71349. Ref.2
Sequence conflict6081R → C in AAB71349. Ref.2
Sequence conflict6451L → F in AAB71349. Ref.2
Sequence conflict6461S → T in AAB83973. Ref.1
Sequence conflict6561V → L in AAB83973. Ref.1
Sequence conflict6871L → F in AAB71349. Ref.2
Sequence conflict6911L → F in AAB71349. Ref.2
Sequence conflict696 – 6972FI → SS in AAB71349. Ref.2
Sequence conflict7171E → K in AAB83973. Ref.1
Sequence conflict8121R → Q in AAB83973. Ref.1
Sequence conflict8411F → L in AAB71349. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 24, 2009. Version 3.
Checksum: 26FE455583D7D35F

FASTA897101,388
        10         20         30         40         50         60 
MDWQPDEQGL QQVLQLLKDS QSPNTATQRI VQDKLKQLNQ FPDFNNYLIF VLTRLKSEDE 

        70         80         90        100        110        120 
PTRSLSGLIL KNNVKAHYQS FPPPVADFIK QECLNNIGDA SSLIRATIGI LITTIASKGE 

       130        140        150        160        170        180 
LQMWPELLPQ LCNLLNSEDY NTCEGAFGAL QKICEDSSEL LDSDALNRPL NIMIPKFLQF 

       190        200        210        220        230        240 
FKHCSPKIRS HAIACVNQFI MDRAQALMDN IDTFIEHLFA LAVDDDPEVR KNVCRALVML 

       250        260        270        280        290        300 
LEVRIDRLIP HMHSIIQYML QRTQDHDENV ALEACEFWLT LAEQPICKEV LASHLVQLIP 

       310        320        330        340        350        360 
ILVNGMKYSE IDIILLKGDV EEDEAVPDSE QDIKPRFHKS RTVTLPHEAE RPDGSEDAED 

       370        380        390        400        410        420 
DDDDDALSDW NLRKCSAAAL DVLANVFREE LLPHLLPLLK GLLFHPEWVV KESGILVLGA 

       430        440        450        460        470        480 
IAEGCMQGMV PYLPELIPHL IQCLSDKKAL VRSIACWTLS RYAHWVVSQP PDMHLKPLMT 

       490        500        510        520        530        540 
ELLKRILDGN KRVQEAACSA FATLEEEACT ELVPYLSYIL DTLVFAFGKY QHKNLLILYD 

       550        560        570        580        590        600 
AIGTLADSVG HHLNQPEYIQ KLMPPLIQKW NELKDEDKDL FPLLECLSSV ATALQSGFLP 

       610        620        630        640        650        660 
YCEPVYQRCV TLVQKTLAQA MMYTQHPEQY EAPDKDFMIV ALDLLSGLAE GLGGHVEQLV 

       670        680        690        700        710        720 
ARSNIMTLLF QCMQDSMPEV RQSSFALLGD LTKACFIHVK PCIAEFMPIL GTNLNPEFIS 

       730        740        750        760        770        780 
VCNNATWAIG EICMQMGAEM QPYVQMVLNN LVEIINRPNT PKTLLENTGR LTSPSAIPAI 

       790        800        810        820        830        840 
TIGRLGYVCP QEVAPMLQQF IRPWCTSLRN IRDNEEKDSA FRGICMMIGV NPGGVVQDFI 

       850        860        870        880        890 
FFCDAVASWV SPKDDLRDMF YKILHGFKDQ VGEDNWQQFS EQFPPLLKER LAAFYGV 

« Hide

Isoform 2 (beta2b) [UniParc].

Checksum: AE64FDCE4DA901DB
Show »

FASTA887100,408

References

« Hide 'large scale' references
[1]"Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins."
Siomi M.C., Eder P.S., Kataoka N., Wan L., Liu Q., Dreyfuss G.
J. Cell Biol. 138:1181-1192(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Human karyopherin beta2b: an homolog of human karyopherin beta2."
Bonifaci N., Radu A., Blobel G.
Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: PNS.
[6]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-862, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Diagnostic exome sequencing in persons with severe intellectual disability."
de Ligt J., Willemsen M.H., van Bon B.W., Kleefstra T., Yntema H.G., Kroes T., Vulto-van Silfhout A.T., Koolen D.A., de Vries P., Gilissen C., del Rosario M., Hoischen A., Scheffer H., de Vries B.B., Brunner H.G., Veltman J.A., Vissers L.E.
N. Engl. J. Med. 367:1921-1929(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT CYS-370.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF019039 mRNA. Translation: AAB83973.1.
AF007748 mRNA. Translation: AAB71349.1.
AC018761 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84300.1.
BC072420 mRNA. Translation: AAH72420.1.
CCDSCCDS45991.1. [O14787-1]
CCDS45992.1. [O14787-2]
RefSeqNP_001129667.1. NM_001136195.1. [O14787-2]
NP_001129668.1. NM_001136196.1. [O14787-1]
NP_038461.2. NM_013433.4. [O14787-2]
UniGeneHs.416049.
Hs.714402.

3D structure databases

ProteinModelPortalO14787.
SMRO14787. Positions 3-896.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119024. 10 interactions.
IntActO14787. 5 interactions.
MINTMINT-2998321.
STRING9606.ENSP00000397379.

PTM databases

PhosphoSiteO14787.

Proteomic databases

MaxQBO14787.
PaxDbO14787.
PRIDEO14787.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000356861; ENSP00000349321; ENSG00000105576. [O14787-2]
ENST00000425528; ENSP00000407182; ENSG00000105576. [O14787-1]
ENST00000441499; ENSP00000389648; ENSG00000105576. [O14787-2]
ENST00000450764; ENSP00000397379; ENSG00000105576. [O14787-2]
ENST00000588216; ENSP00000465625; ENSG00000105576. [O14787-2]
ENST00000592287; ENSP00000468434; ENSG00000105576. [O14787-1]
GeneID30000.
KEGGhsa:30000.
UCSCuc002muo.3. human. [O14787-1]
uc002muq.3. human. [O14787-2]

Organism-specific databases

CTD30000.
GeneCardsGC19M012810.
HGNCHGNC:19998. TNPO2.
HPACAB046446.
MIM603002. gene.
neXtProtNX_O14787.
PharmGKBPA134921349.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5215.
HOGENOMHOG000203940.
HOVERGENHBG058963.
InParanoidO14787.
OMAVRRHVCQ.
OrthoDBEOG7XM2X0.
PhylomeDBO14787.
TreeFamTF300825.

Gene expression databases

ArrayExpressO14787.
BgeeO14787.
CleanExHS_TNPO2.
GenevestigatorO14787.

Family and domain databases

Gene3D1.25.10.10. 2 hits.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
IPR001494. Importin-beta_N.
[Graphical view]
PfamPF02985. HEAT. 1 hit.
PF03810. IBN_N. 1 hit.
[Graphical view]
SMARTSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
ProtoNetSearch...

Other

ChiTaRSTNPO2. human.
GeneWikiTNPO2.
GenomeRNAi30000.
NextBio52812.
PROO14787.
SOURCESearch...

Entry information

Entry nameTNPO2_HUMAN
AccessionPrimary (citable) accession number: O14787
Secondary accession number(s): O14655, Q6IN77
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: November 24, 2009
Last modified: July 9, 2014
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM