O14787 (TNPO2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transportin-2

Alternative name(s):
Karyopherin beta-2b

Gene names

Name:

TNPO2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	897 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the importin beta family. Contains 13 HEAT repeats. Contains 1 importin N-terminal domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular protein transport Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Traceable author statement Ref.1. Source: ProtInc nuclear pore Inferred from electronic annotation. Source: InterPro nucleus Traceable author statement Ref.1. Source: ProtInc
Molecular_function	nuclear localization sequence binding Traceable author statement Ref.1. Source: ProtInc protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O14787-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O14787-2)

Also known as: beta2b;

The sequence of this isoform differs from the canonical sequence as follows:
     769-778: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 897	897	Transportin-2		PRO_0000120767
Regions
	Domain	31 – 99	69	Importin N-terminal
	Repeat	121 – 159	39	HEAT 1
	Repeat	167 – 205	39	HEAT 2
	Repeat	208 – 246	39	HEAT 3
	Repeat	249 – 287	39	HEAT 4
	Repeat	292 – 330	39	HEAT 5
	Repeat	389 – 427	39	HEAT 6
	Repeat	430 – 468	39	HEAT 7
	Repeat	472 – 510	39	HEAT 8
	Repeat	513 – 551	39	HEAT 9
	Repeat	556 – 596	41	HEAT 10
	Repeat	659 – 697	39	HEAT 11
	Repeat	700 – 738	39	HEAT 12
	Repeat	741 – 780	40	HEAT 13
Amino acid modifications
	Modified residue	862	1	N6-acetyllysine Ref.6
Natural variations
	Alternative sequence	769 – 778	10	Missing in isoform 2.		VSP_009657
Experimental info
	Sequence conflict	28	1	Q → H in AAB71349. Ref.2
	Sequence conflict	32	1	Q → R in AAB71349. Ref.2
	Sequence conflict	194	1	A → G in AAB71349. Ref.2
	Sequence conflict	398 – 401	4	LLKG → YQS in AAB83973. Ref.1
	Sequence conflict	492	1	R → K in AAB71349. Ref.2
	Sequence conflict	499	1	S → I in AAB71349. Ref.2
	Sequence conflict	507	1	E → K in AAB71349. Ref.2
	Sequence conflict	608	1	R → C in AAB71349. Ref.2
	Sequence conflict	645	1	L → F in AAB71349. Ref.2
	Sequence conflict	646	1	S → T in AAB83973. Ref.1
	Sequence conflict	656	1	V → L in AAB83973. Ref.1
	Sequence conflict	687	1	L → F in AAB71349. Ref.2
	Sequence conflict	691	1	L → F in AAB71349. Ref.2
	Sequence conflict	696 – 697	2	FI → SS in AAB71349. Ref.2
	Sequence conflict	717	1	E → K in AAB83973. Ref.1
	Sequence conflict	812	1	R → Q in AAB83973. Ref.1
	Sequence conflict	841	1	F → L in AAB71349. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 24, 2009. Version 3. Checksum: 26FE455583D7D35F	FASTA	897	101,388
	10 20 30 40 50 60 MDWQPDEQGL QQVLQLLKDS QSPNTATQRI VQDKLKQLNQ FPDFNNYLIF VLTRLKSEDE 70 80 90 100 110 120 PTRSLSGLIL KNNVKAHYQS FPPPVADFIK QECLNNIGDA SSLIRATIGI LITTIASKGE 130 140 150 160 170 180 LQMWPELLPQ LCNLLNSEDY NTCEGAFGAL QKICEDSSEL LDSDALNRPL NIMIPKFLQF 190 200 210 220 230 240 FKHCSPKIRS HAIACVNQFI MDRAQALMDN IDTFIEHLFA LAVDDDPEVR KNVCRALVML 250 260 270 280 290 300 LEVRIDRLIP HMHSIIQYML QRTQDHDENV ALEACEFWLT LAEQPICKEV LASHLVQLIP 310 320 330 340 350 360 ILVNGMKYSE IDIILLKGDV EEDEAVPDSE QDIKPRFHKS RTVTLPHEAE RPDGSEDAED 370 380 390 400 410 420 DDDDDALSDW NLRKCSAAAL DVLANVFREE LLPHLLPLLK GLLFHPEWVV KESGILVLGA 430 440 450 460 470 480 IAEGCMQGMV PYLPELIPHL IQCLSDKKAL VRSIACWTLS RYAHWVVSQP PDMHLKPLMT 490 500 510 520 530 540 ELLKRILDGN KRVQEAACSA FATLEEEACT ELVPYLSYIL DTLVFAFGKY QHKNLLILYD 550 560 570 580 590 600 AIGTLADSVG HHLNQPEYIQ KLMPPLIQKW NELKDEDKDL FPLLECLSSV ATALQSGFLP 610 620 630 640 650 660 YCEPVYQRCV TLVQKTLAQA MMYTQHPEQY EAPDKDFMIV ALDLLSGLAE GLGGHVEQLV 670 680 690 700 710 720 ARSNIMTLLF QCMQDSMPEV RQSSFALLGD LTKACFIHVK PCIAEFMPIL GTNLNPEFIS 730 740 750 760 770 780 VCNNATWAIG EICMQMGAEM QPYVQMVLNN LVEIINRPNT PKTLLENTGR LTSPSAIPAI 790 800 810 820 830 840 TIGRLGYVCP QEVAPMLQQF IRPWCTSLRN IRDNEEKDSA FRGICMMIGV NPGGVVQDFI 850 860 870 880 890 FFCDAVASWV SPKDDLRDMF YKILHGFKDQ VGEDNWQQFS EQFPPLLKER LAAFYGV
	Isoform 2 (beta2b) [UniParc]. Checksum: AE64FDCE4DA901DB	FASTA	887	100,408
	10 20 30 40 50 60 MDWQPDEQGL QQVLQLLKDS QSPNTATQRI VQDKLKQLNQ FPDFNNYLIF VLTRLKSEDE 70 80 90 100 110 120 PTRSLSGLIL KNNVKAHYQS FPPPVADFIK QECLNNIGDA SSLIRATIGI LITTIASKGE 130 140 150 160 170 180 LQMWPELLPQ LCNLLNSEDY NTCEGAFGAL QKICEDSSEL LDSDALNRPL NIMIPKFLQF 190 200 210 220 230 240 FKHCSPKIRS HAIACVNQFI MDRAQALMDN IDTFIEHLFA LAVDDDPEVR KNVCRALVML 250 260 270 280 290 300 LEVRIDRLIP HMHSIIQYML QRTQDHDENV ALEACEFWLT LAEQPICKEV LASHLVQLIP 310 320 330 340 350 360 ILVNGMKYSE IDIILLKGDV EEDEAVPDSE QDIKPRFHKS RTVTLPHEAE RPDGSEDAED 370 380 390 400 410 420 DDDDDALSDW NLRKCSAAAL DVLANVFREE LLPHLLPLLK GLLFHPEWVV KESGILVLGA 430 440 450 460 470 480 IAEGCMQGMV PYLPELIPHL IQCLSDKKAL VRSIACWTLS RYAHWVVSQP PDMHLKPLMT 490 500 510 520 530 540 ELLKRILDGN KRVQEAACSA FATLEEEACT ELVPYLSYIL DTLVFAFGKY QHKNLLILYD 550 560 570 580 590 600 AIGTLADSVG HHLNQPEYIQ KLMPPLIQKW NELKDEDKDL FPLLECLSSV ATALQSGFLP 610 620 630 640 650 660 YCEPVYQRCV TLVQKTLAQA MMYTQHPEQY EAPDKDFMIV ALDLLSGLAE GLGGHVEQLV 670 680 690 700 710 720 ARSNIMTLLF QCMQDSMPEV RQSSFALLGD LTKACFIHVK PCIAEFMPIL GTNLNPEFIS 730 740 750 760 770 780 VCNNATWAIG EICMQMGAEM QPYVQMVLNN LVEIINRPNT PKTLLENTAI TIGRLGYVCP 790 800 810 820 830 840 QEVAPMLQQF IRPWCTSLRN IRDNEEKDSA FRGICMMIGV NPGGVVQDFI FFCDAVASWV 850 860 870 880 SPKDDLRDMF YKILHGFKDQ VGEDNWQQFS EQFPPLLKER LAAFYGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins." Siomi M.C., Eder P.S., Kataoka N., Wan L., Liu Q., Dreyfuss G. J. Cell Biol. 138:1181-1192(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Human karyopherin beta2b: an homolog of human karyopherin beta2." Bonifaci N., Radu A., Blobel G. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: PNS.
[6]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-862, MASS SPECTROMETRY.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF019039 mRNA. Translation: AAB83973.1. AF007748 mRNA. Translation: AAB71349.1. AC018761 Genomic DNA. No translation available. CH471106 Genomic DNA. Translation: EAW84300.1. BC072420 mRNA. Translation: AAH72420.1.
IPI	IPI00164417. IPI00409698.
RefSeq	NP_001129667.1. NM_001136195.1. NP_001129668.1. NM_001136196.1. NP_038461.2. NM_013433.4.
UniGene	Hs.416049.
3D structure databases
ProteinModelPortal	O14787.
SMR	O14787. Positions 3-896.
ModBase	Search...
Protein-protein interaction databases
IntAct	O14787. 3 interactions.
STRING	9606.ENSP00000397379.
PTM databases
PhosphoSite	O14787.
Proteomic databases
PaxDb	O14787.
PRIDE	O14787.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000356861; ENSP00000349321; ENSG00000105576. ENST00000425528; ENSP00000407182; ENSG00000105576. ENST00000441499; ENSP00000389648; ENSG00000105576. ENST00000450764; ENSP00000397379; ENSG00000105576. ENST00000588216; ENSP00000465625; ENSG00000105576. ENST00000592287; ENSP00000468434; ENSG00000105576.
GeneID	30000.
KEGG	hsa:30000.
UCSC	uc002muo.3. human.
Organism-specific databases
CTD	30000.
GeneCards	GC19M012810.
HGNC	HGNC:19998. TNPO2.
HPA	CAB046446.
MIM	603002. gene.
neXtProt	NX_O14787.
PharmGKB	PA134921349.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5215.
HOGENOM	HOG000203940.
HOVERGEN	HBG058963.
InParanoid	O14787.
OrthoDB	EOG4T4CTR.
PhylomeDB	O14787.
Gene expression databases
ArrayExpress	O14787.
Bgee	O14787.
CleanEx	HS_TNPO2.
Genevestigator	O14787.
GermOnline	ENSG00000105576. Homo sapiens.
Family and domain databases
Gene3D	1.25.10.10. 2 hits.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR000357. HEAT. IPR001494. Importin-beta_N. [Graphical view]
Pfam	PF02985. HEAT. 1 hit. PF03810. IBN_N. 1 hit. [Graphical view]
SMART	SM00913. IBN_N. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50077. HEAT_REPEAT. False negative. PS50166. IMPORTIN_B_NT. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TNPO2. human.
GenomeRNAi	30000.
NextBio	52812.
SOURCE	Search...

Entry information

Entry name

TNPO2_HUMAN

Accession

Primary (citable) accession number: O14787
Secondary accession number(s): O14655, Q6IN77

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	November 24, 2009
Last modified:	May 1, 2013
This is version 101 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.