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Protein

Kinetochore protein NDC80 homolog

Gene

NDC80

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:9315664, PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020). Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules (PubMed:25743205, PubMed:23891108).12 Publications

GO - Molecular functioni

GO - Biological processi

  • attachment of mitotic spindle microtubules to kinetochore Source: UniProtKB
  • attachment of spindle microtubules to kinetochore Source: UniProtKB
  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: UniProtKB
  • establishment of mitotic spindle orientation Source: UniProtKB
  • metaphase plate congression Source: UniProtKB
  • mitotic nuclear division Source: ProtInc
  • mitotic sister chromatid segregation Source: ProtInc
  • mitotic spindle organization Source: UniProtKB
  • positive regulation of mitotic cell cycle spindle assembly checkpoint Source: UniProtKB
  • sister chromatid cohesion Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciZFISH:ENSG00000080986-MONOMER.
ReactomeiR-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-68877. Mitotic Prometaphase.
SignaLinkiO14777.
SIGNORiO14777.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein NDC80 homolog
Alternative name(s):
Highly expressed in cancer protein
Kinetochore protein Hec1
Short name:
HsHec1
Kinetochore-associated protein 2
Retinoblastoma-associated protein HEC
Gene namesi
Name:NDC80
Synonyms:HEC, HEC1, KNTC2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:16909. NDC80.

Subcellular locationi

GO - Cellular componenti

  • chromosome, centromeric region Source: ProtInc
  • condensed chromosome kinetochore Source: UniProtKB
  • condensed nuclear chromosome outer kinetochore Source: BHF-UCL
  • cytosol Source: Reactome
  • kinetochore Source: UniProtKB
  • membrane Source: UniProtKB
  • Ndc80 complex Source: UniProtKB
  • nucleus Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi234E → K: Abrogates binding to RB1. 1 Publication1

Organism-specific databases

DisGeNETi10403.
OpenTargetsiENSG00000080986.
PharmGKBiPA162397359.

Chemistry databases

ChEMBLiCHEMBL5660.

Polymorphism and mutation databases

BioMutaiNDC80.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002495501 – 642Kinetochore protein NDC80 homologAdd BLAST642

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei44PhosphoserineCombined sources1
Modified residuei69PhosphoserineCombined sources1
Modified residuei165Phosphoserine; by NEK21 Publication1
Modified residuei242PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO14777.
MaxQBiO14777.
PaxDbiO14777.
PeptideAtlasiO14777.
PRIDEiO14777.

PTM databases

iPTMnetiO14777.
PhosphoSitePlusiO14777.

Expressioni

Developmental stagei

Expression peaks in mitosis.3 Publications

Gene expression databases

BgeeiENSG00000080986.
CleanExiHS_NDC80.
ExpressionAtlasiO14777. baseline and differential.
GenevisibleiO14777. HS.

Organism-specific databases

HPAiHPA031152.
HPA049560.

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. Interacts with isoform 1 of NEK2 and ZWINT specifically during mitosis. Interacts with CENPH and MIS12. May interact with AURKB, PSMC2, PSMC5 and SMC1A. May interact with RB1 during G2 phase and mitosis. Interacts with CKAP5.11 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-715849,EBI-715849
ATF4P188485EBI-715849,EBI-492498
ATP5OP480473EBI-715849,EBI-355815
CALCOCO1Q9P1Z24EBI-715849,EBI-749920
CCDC53Q9Y3C06EBI-715849,EBI-712969
CCHCR1Q8TD31-37EBI-715849,EBI-10175300
CCNHP519463EBI-715849,EBI-741406
CCNKO759093EBI-715849,EBI-739806
CENPHQ9H3R53EBI-715849,EBI-1003700
HAUS1Q96CS23EBI-715849,EBI-2514791
HGSO149645EBI-715849,EBI-740220
IFT20Q8IY315EBI-715849,EBI-744203
KLC3Q6P5973EBI-715849,EBI-1643885
KRT6BP042593EBI-715849,EBI-740907
MED4Q9NPJ63EBI-715849,EBI-394607
MFAP1P550813EBI-715849,EBI-1048159
NDEL1Q9GZM83EBI-715849,EBI-928842
NUF2Q9BZD413EBI-715849,EBI-724102
RUFY4Q6ZNE95EBI-715849,EBI-10181525
SFR1Q86XK33EBI-715849,EBI-1104535
SGF29Q96ES76EBI-715849,EBI-743117
SPC25Q9HBM110EBI-715849,EBI-999909
STX11O755583EBI-715849,EBI-714135
TFIP11Q9UBB95EBI-715849,EBI-1105213
TNIP1Q150255EBI-715849,EBI-357849
TP53BP2Q13625-33EBI-715849,EBI-10175039
TXLNAP402223EBI-715849,EBI-359793
USHBP1Q8N6Y03EBI-715849,EBI-739895
ZWINTO9522913EBI-715849,EBI-1001132

Protein-protein interaction databases

BioGridi115675. 80 interactors.
DIPiDIP-35100N.
IntActiO14777. 74 interactors.
MINTiMINT-1411676.
STRINGi9606.ENSP00000261597.

Structurei

Secondary structure

1642
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi89 – 105Combined sources17
Turni114 – 117Combined sources4
Helixi122 – 133Combined sources12
Turni134 – 136Combined sources3
Helixi147 – 157Combined sources11
Helixi166 – 170Combined sources5
Turni171 – 173Combined sources3
Turni175 – 177Combined sources3
Helixi178 – 194Combined sources17
Helixi222 – 238Combined sources17
Helixi244 – 258Combined sources15
Helixi264 – 286Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IGPX-ray1.80A81-196[»]
2VE7X-ray2.88A/B80-286[»]
3IZ0electron microscopy-C/E80-286[»]
ProteinModelPortaliO14777.
SMRiO14777.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO14777.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 445Interaction with the N-terminus of CDCA1Add BLAST445
Regioni1 – 250Nuclear localizationAdd BLAST250
Regioni128 – 251Interaction with RB1Add BLAST124
Regioni251 – 618Interaction with NEK2 and ZWINT1 PublicationAdd BLAST368
Regioni251 – 431Interaction with SMC1AAdd BLAST181
Regioni361 – 547Interaction with PSMC2 and SMC1AAdd BLAST187
Regioni446 – 642Interaction with the C-terminus of CDCA1 and the SPBC24-SPBC25 subcomplexAdd BLAST197

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili261 – 403Sequence analysisAdd BLAST143
Coiled coili458 – 642Sequence analysisAdd BLAST185

Sequence similaritiesi

Belongs to the NDC80/HEC1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0995. Eukaryota.
COG5185. LUCA.
GeneTreeiENSGT00390000018386.
HOGENOMiHOG000012981.
HOVERGENiHBG081861.
InParanoidiO14777.
KOiK11547.
OMAiDEKCASE.
OrthoDBiEOG091G046M.
PhylomeDBiO14777.
TreeFamiTF101177.

Family and domain databases

InterProiIPR005550. Kinetochore_Ndc80.
[Graphical view]
PANTHERiPTHR10643. PTHR10643. 1 hit.
PfamiPF03801. Ndc80_HEC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O14777-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRSSVSSGG AGRLSMQELR SQDVNKQGLY TPQTKEKPTF GKLSINKPTS
60 70 80 90 100
ERKVSLFGKR TSGHGSRNSQ LGIFSSSEKI KDPRPLNDKA FIQQCIRQLC
110 120 130 140 150
EFLTENGYAH NVSMKSLQAP SVKDFLKIFT FLYGFLCPSY ELPDTKFEEE
160 170 180 190 200
VPRIFKDLGY PFALSKSSMY TVGAPHTWPH IVAALVWLID CIKIHTAMKE
210 220 230 240 250
SSPLFDDGQP WGEETEDGIM HNKLFLDYTI KCYESFMSGA DSFDEMNAEL
260 270 280 290 300
QSKLKDLFNV DAFKLESLEA KNRALNEQIA RLEQEREKEP NRLESLRKLK
310 320 330 340 350
ASLQGDVQKY QAYMSNLESH SAILDQKLNG LNEEIARVEL ECETIKQENT
360 370 380 390 400
RLQNIIDNQK YSVADIERIN HERNELQQTI NKLTKDLEAE QQKLWNEELK
410 420 430 440 450
YARGKEAIET QLAEYHKLAR KLKLIPKGAE NSKGYDFEIK FNPEAGANCL
460 470 480 490 500
VKYRAQVYVP LKELLNETEE EINKALNKKM GLEDTLEQLN AMITESKRSV
510 520 530 540 550
RTLKEEVQKL DDLYQQKIKE AEEEDEKCAS ELESLEKHKH LLESTVNQGL
560 570 580 590 600
SEAMNELDAV QREYQLVVQT TTEERRKVGN NLQRLLEMVA THVGSVEKHL
610 620 630 640
EEQIAKVDRE YEECMSEDLS ENIKEIRDKY EKKATLIKSS EE
Length:642
Mass (Da):73,913
Last modified:January 1, 1998 - v1
Checksum:i6A82DA4D8B77ECDC
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02743666S → A.Corresponds to variant rs16943490dbSNPEnsembl.1
Natural variantiVAR_027437348E → D.Corresponds to variant rs12456560dbSNPEnsembl.1
Natural variantiVAR_027438605A → P.Corresponds to variant rs1983346dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017790 mRNA. Translation: AAB80726.1.
BC010171 mRNA. No translation available.
CCDSiCCDS11827.1.
RefSeqiNP_006092.1. NM_006101.2.
UniGeneiHs.414407.

Genome annotation databases

EnsembliENST00000261597; ENSP00000261597; ENSG00000080986.
GeneIDi10403.
KEGGihsa:10403.
UCSCiuc002kli.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017790 mRNA. Translation: AAB80726.1.
BC010171 mRNA. No translation available.
CCDSiCCDS11827.1.
RefSeqiNP_006092.1. NM_006101.2.
UniGeneiHs.414407.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IGPX-ray1.80A81-196[»]
2VE7X-ray2.88A/B80-286[»]
3IZ0electron microscopy-C/E80-286[»]
ProteinModelPortaliO14777.
SMRiO14777.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115675. 80 interactors.
DIPiDIP-35100N.
IntActiO14777. 74 interactors.
MINTiMINT-1411676.
STRINGi9606.ENSP00000261597.

Chemistry databases

ChEMBLiCHEMBL5660.

PTM databases

iPTMnetiO14777.
PhosphoSitePlusiO14777.

Polymorphism and mutation databases

BioMutaiNDC80.

Proteomic databases

EPDiO14777.
MaxQBiO14777.
PaxDbiO14777.
PeptideAtlasiO14777.
PRIDEiO14777.

Protocols and materials databases

DNASUi10403.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261597; ENSP00000261597; ENSG00000080986.
GeneIDi10403.
KEGGihsa:10403.
UCSCiuc002kli.3. human.

Organism-specific databases

CTDi10403.
DisGeNETi10403.
GeneCardsiNDC80.
HGNCiHGNC:16909. NDC80.
HPAiHPA031152.
HPA049560.
MIMi607272. gene.
neXtProtiNX_O14777.
OpenTargetsiENSG00000080986.
PharmGKBiPA162397359.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0995. Eukaryota.
COG5185. LUCA.
GeneTreeiENSGT00390000018386.
HOGENOMiHOG000012981.
HOVERGENiHBG081861.
InParanoidiO14777.
KOiK11547.
OMAiDEKCASE.
OrthoDBiEOG091G046M.
PhylomeDBiO14777.
TreeFamiTF101177.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000080986-MONOMER.
ReactomeiR-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-68877. Mitotic Prometaphase.
SignaLinkiO14777.
SIGNORiO14777.

Miscellaneous databases

ChiTaRSiNDC80. human.
EvolutionaryTraceiO14777.
GeneWikiiNDC80.
GenomeRNAii10403.
PROiO14777.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000080986.
CleanExiHS_NDC80.
ExpressionAtlasiO14777. baseline and differential.
GenevisibleiO14777. HS.

Family and domain databases

InterProiIPR005550. Kinetochore_Ndc80.
[Graphical view]
PANTHERiPTHR10643. PTHR10643. 1 hit.
PfamiPF03801. Ndc80_HEC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDC80_HUMAN
AccessioniPrimary (citable) accession number: O14777
Secondary accession number(s): Q6PJX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.