Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Kinetochore protein NDC80 homolog

Gene

NDC80

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:9315664, PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020). Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules (PubMed:25743205, PubMed:23891108).12 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • structural constituent of cytoskeleton Source: GO_Central

GO - Biological processi

  • attachment of mitotic spindle microtubules to kinetochore Source: UniProtKB
  • attachment of spindle microtubules to kinetochore Source: UniProtKB
  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: UniProtKB
  • establishment of mitotic spindle orientation Source: UniProtKB
  • kinetochore organization Source: CAFA
  • metaphase plate congression Source: UniProtKB
  • mitotic cell cycle Source: ProtInc
  • mitotic sister chromatid segregation Source: ProtInc
  • mitotic spindle organization Source: UniProtKB
  • positive regulation of mitotic cell cycle spindle assembly checkpoint Source: UniProtKB
  • positive regulation of protein localization to kinetochore Source: CAFA
  • sister chromatid cohesion Source: Reactome

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-141444. Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
R-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-68877. Mitotic Prometaphase.
SignaLinkiO14777.
SIGNORiO14777.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein NDC80 homolog
Alternative name(s):
Highly expressed in cancer protein
Kinetochore protein Hec1
Short name:
HsHec1
Kinetochore-associated protein 2
Retinoblastoma-associated protein HEC
Gene namesi
Name:NDC80
Synonyms:HEC, HEC1, KNTC2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

EuPathDBiHostDB:ENSG00000080986.12.
HGNCiHGNC:16909. NDC80.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi234E → K: Abrogates binding to RB1. 1 Publication1

Organism-specific databases

DisGeNETi10403.
OpenTargetsiENSG00000080986.
PharmGKBiPA162397359.

Chemistry databases

ChEMBLiCHEMBL5660.

Polymorphism and mutation databases

BioMutaiNDC80.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002495501 – 642Kinetochore protein NDC80 homologAdd BLAST642

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei44PhosphoserineCombined sources1
Modified residuei69PhosphoserineCombined sources1
Modified residuei165Phosphoserine; by NEK21 Publication1
Modified residuei242PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO14777.
MaxQBiO14777.
PaxDbiO14777.
PeptideAtlasiO14777.
PRIDEiO14777.

PTM databases

iPTMnetiO14777.
PhosphoSitePlusiO14777.

Expressioni

Developmental stagei

Expression peaks in mitosis.3 Publications

Gene expression databases

BgeeiENSG00000080986.
CleanExiHS_NDC80.
ExpressionAtlasiO14777. baseline and differential.
GenevisibleiO14777. HS.

Organism-specific databases

HPAiHPA066330.

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. Interacts with isoform 1 of NEK2 and ZWINT specifically during mitosis. Interacts with CENPH and MIS12. May interact with AURKB, PSMC2, PSMC5 and SMC1A. May interact with RB1 during G2 phase and mitosis. Interacts with CKAP5 (PubMed:27156448). Interacts with CDT1; leading to kinetochore localization of CDT1 (PubMed:22581055).12 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi115675. 87 interactors.
CORUMiO14777.
DIPiDIP-35100N.
IntActiO14777. 78 interactors.
MINTiMINT-1411676.
STRINGi9606.ENSP00000261597.

Structurei

Secondary structure

1642
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi89 – 105Combined sources17
Turni114 – 117Combined sources4
Helixi122 – 133Combined sources12
Turni134 – 136Combined sources3
Helixi147 – 157Combined sources11
Helixi166 – 170Combined sources5
Turni171 – 173Combined sources3
Turni175 – 177Combined sources3
Helixi178 – 194Combined sources17
Helixi222 – 238Combined sources17
Helixi244 – 258Combined sources15
Helixi264 – 288Combined sources25

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IGPX-ray1.80A81-196[»]
2VE7X-ray2.88A/B80-286[»]
3IZ0electron microscopy-C/E80-286[»]
ProteinModelPortaliO14777.
SMRiO14777.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO14777.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 445Interaction with the N-terminus of CDCA1Add BLAST445
Regioni1 – 250Nuclear localizationAdd BLAST250
Regioni128 – 251Interaction with RB1Add BLAST124
Regioni251 – 618Interaction with NEK2 and ZWINT1 PublicationAdd BLAST368
Regioni251 – 431Interaction with SMC1AAdd BLAST181
Regioni361 – 547Interaction with PSMC2 and SMC1AAdd BLAST187
Regioni446 – 642Interaction with the C-terminus of CDCA1 and the SPBC24-SPBC25 subcomplexAdd BLAST197

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili261 – 403Sequence analysisAdd BLAST143
Coiled coili458 – 642Sequence analysisAdd BLAST185

Sequence similaritiesi

Belongs to the NDC80/HEC1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0995. Eukaryota.
COG5185. LUCA.
GeneTreeiENSGT00390000018386.
HOGENOMiHOG000012981.
HOVERGENiHBG081861.
InParanoidiO14777.
KOiK11547.
OMAiKSPTQKD.
OrthoDBiEOG091G046M.
PhylomeDBiO14777.
TreeFamiTF101177.

Family and domain databases

InterProiView protein in InterPro
IPR005550. Kinetochore_Ndc80.
PfamiView protein in Pfam
PF03801. Ndc80_HEC. 1 hit.

Sequencei

Sequence statusi: Complete.

O14777-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRSSVSSGG AGRLSMQELR SQDVNKQGLY TPQTKEKPTF GKLSINKPTS
60 70 80 90 100
ERKVSLFGKR TSGHGSRNSQ LGIFSSSEKI KDPRPLNDKA FIQQCIRQLC
110 120 130 140 150
EFLTENGYAH NVSMKSLQAP SVKDFLKIFT FLYGFLCPSY ELPDTKFEEE
160 170 180 190 200
VPRIFKDLGY PFALSKSSMY TVGAPHTWPH IVAALVWLID CIKIHTAMKE
210 220 230 240 250
SSPLFDDGQP WGEETEDGIM HNKLFLDYTI KCYESFMSGA DSFDEMNAEL
260 270 280 290 300
QSKLKDLFNV DAFKLESLEA KNRALNEQIA RLEQEREKEP NRLESLRKLK
310 320 330 340 350
ASLQGDVQKY QAYMSNLESH SAILDQKLNG LNEEIARVEL ECETIKQENT
360 370 380 390 400
RLQNIIDNQK YSVADIERIN HERNELQQTI NKLTKDLEAE QQKLWNEELK
410 420 430 440 450
YARGKEAIET QLAEYHKLAR KLKLIPKGAE NSKGYDFEIK FNPEAGANCL
460 470 480 490 500
VKYRAQVYVP LKELLNETEE EINKALNKKM GLEDTLEQLN AMITESKRSV
510 520 530 540 550
RTLKEEVQKL DDLYQQKIKE AEEEDEKCAS ELESLEKHKH LLESTVNQGL
560 570 580 590 600
SEAMNELDAV QREYQLVVQT TTEERRKVGN NLQRLLEMVA THVGSVEKHL
610 620 630 640
EEQIAKVDRE YEECMSEDLS ENIKEIRDKY EKKATLIKSS EE
Length:642
Mass (Da):73,913
Last modified:January 1, 1998 - v1
Checksum:i6A82DA4D8B77ECDC
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02743666S → A. Corresponds to variant dbSNP:rs16943490Ensembl.1
Natural variantiVAR_027437348E → D. Corresponds to variant dbSNP:rs12456560Ensembl.1
Natural variantiVAR_027438605A → P. Corresponds to variant dbSNP:rs1983346Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017790 mRNA. Translation: AAB80726.1.
BC010171 mRNA. No translation available.
CCDSiCCDS11827.1.
RefSeqiNP_006092.1. NM_006101.2.
UniGeneiHs.414407.

Genome annotation databases

EnsembliENST00000261597; ENSP00000261597; ENSG00000080986.
GeneIDi10403.
KEGGihsa:10403.
UCSCiuc002kli.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiNDC80_HUMAN
AccessioniPrimary (citable) accession number: O14777
Secondary accession number(s): Q6PJX2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: January 1, 1998
Last modified: September 27, 2017
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families