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O14775

- GBB5_HUMAN

UniProt

O14775 - GBB5_HUMAN

Protein

Guanine nucleotide-binding protein subunit beta-5

Gene

GNB5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 141 (01 Oct 2014)
      Sequence version 2 (31 Oct 2003)
      Previous versions | rss
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    Functioni

    Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

    GO - Molecular functioni

    1. chaperone binding Source: UniProt
    2. G-protein gamma-subunit binding Source: UniProt
    3. GTPase activity Source: UniProtKB
    4. protein binding Source: UniProt
    5. signal transducer activity Source: UniProtKB

    GO - Biological processi

    1. GTP catabolic process Source: GOC
    2. negative regulation of voltage-gated calcium channel activity Source: UniProt
    3. phototransduction, visible light Source: Reactome
    4. regulation of rhodopsin mediated signaling pathway Source: Reactome
    5. rhodopsin mediated signaling pathway Source: Reactome
    6. signal transduction Source: UniProtKB

    Keywords - Molecular functioni

    Transducer

    Enzyme and pathway databases

    ReactomeiREACT_15457. G-protein activation.
    REACT_163919. Inactivation, recovery and regulation of the phototransduction cascade.
    REACT_172761. Ca2+ pathway.
    REACT_18274. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
    REACT_18283. G alpha (q) signalling events.
    REACT_18339. Adrenaline,noradrenaline inhibits insulin secretion.
    REACT_18377. Glucagon-type ligand receptors.
    REACT_18407. G alpha (12/13) signalling events.
    REACT_19140. ADP signalling through P2Y purinoceptor 1.
    REACT_19145. G beta:gamma signalling through PLC beta.
    REACT_19231. G alpha (i) signalling events.
    REACT_19290. G beta:gamma signalling through PI3Kgamma.
    REACT_19327. G alpha (s) signalling events.
    REACT_19333. G alpha (z) signalling events.
    REACT_20647. Thromboxane signalling through TP receptor.
    REACT_20653. ADP signalling through P2Y purinoceptor 12.
    REACT_21254. Presynaptic function of Kainate receptors.
    REACT_21384. Thrombin signalling through proteinase activated receptors (PARs).
    REACT_23946. Prostacyclin signalling through prostacyclin receptor.
    REACT_24023. Regulation of water balance by renal Aquaporins.
    SignaLinkiO14775.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine nucleotide-binding protein subunit beta-5
    Alternative name(s):
    Gbeta5
    Transducin beta chain 5
    Gene namesi
    Name:GNB5
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:4401. GNB5.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: Ensembl
    2. plasma membrane Source: Ensembl

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA28780.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 395395Guanine nucleotide-binding protein subunit beta-5PRO_0000127705Add
    BLAST

    Proteomic databases

    MaxQBiO14775.
    PaxDbiO14775.
    PRIDEiO14775.

    PTM databases

    PhosphoSiteiO14775.

    Expressioni

    Tissue specificityi

    Expressed in multiple tissues.

    Gene expression databases

    ArrayExpressiO14775.
    BgeeiO14775.
    CleanExiHS_GNB5.
    GenevestigatoriO14775.

    Organism-specific databases

    HPAiCAB032353.
    HPA041942.
    HPA044198.

    Interactioni

    Subunit structurei

    G proteins are composed of 3 units, alpha, beta and gamma. Component of the RGS9-1-Gbeta5 complex composed of RGS9 (isoform RGS9-1), Gbeta5 (GNB5) and RGS9BP By similarity.By similarity

    Protein-protein interaction databases

    BioGridi115920. 26 interactions.
    IntActiO14775. 27 interactions.
    MINTiMINT-1399610.
    STRINGi9606.ENSP00000261837.

    Structurei

    3D structure databases

    ProteinModelPortaliO14775.
    SMRiO14775. Positions 43-395.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati103 – 14240WD 1Add
    BLAST
    Repeati145 – 18440WD 2Add
    BLAST
    Repeati193 – 23442WD 3Add
    BLAST
    Repeati236 – 27843WD 4Add
    BLAST
    Repeati279 – 31840WD 5Add
    BLAST
    Repeati320 – 36243WD 6Add
    BLAST
    Repeati365 – 39430WD 7Add
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat G protein beta family.Curated
    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    HOGENOMiHOG000176356.
    HOVERGENiHBG000188.
    InParanoidiO14775.
    KOiK04539.
    OMAiDQTFLVN.
    OrthoDBiEOG7GN2N5.
    PhylomeDBiO14775.
    TreeFamiTF106149.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR020472. G-protein_beta_WD-40_rep.
    IPR001632. Gprotein_B.
    IPR016346. Guanine_nucleotide-bd_bsu.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 7 hits.
    [Graphical view]
    PIRSFiPIRSF002394. GN-bd_beta. 1 hit.
    PRINTSiPR00319. GPROTEINB.
    PR00320. GPROTEINBRPT.
    SMARTiSM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 3 hits.
    PS50082. WD_REPEATS_2. 6 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O14775-1) [UniParc]FASTAAdd to Basket

    Also known as: Long, Beta-5L

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MCDQTFLVNV FGSCDKCFKQ RALRPVFKKS QQLSYCSTCA EIMATEGLHE    50
    NETLASLKSE AESLKGKLEE ERAKLHDVEL HQVAERVEAL GQFVMKTRRT 100
    LKGHGNKVLC MDWCKDKRRI VSSSQDGKVI VWDSFTTNKE HAVTMPCTWV 150
    MACAYAPSGC AIACGGLDNK CSVYPLTFDK NENMAAKKKS VAMHTNYLSA 200
    CSFTNSDMQI LTASGDGTCA LWDVESGQLL QSFHGHGADV LCLDLAPSET 250
    GNTFVSGGCD KKAMVWDMRS GQCVQAFETH ESDINSVRYY PSGDAFASGS 300
    DDATCRLYDL RADREVAIYS KESIIFGASS VDFSLSGRLL FAGYNDYTIN 350
    VWDVLKGSRV SILFGHENRV STLRVSPDGT AFCSGSWDHT LRVWA 395
    Length:395
    Mass (Da):43,566
    Last modified:October 31, 2003 - v2
    Checksum:iE001B07FCFA587AD
    GO
    Isoform 2 (identifier: O14775-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-42: Missing.

    Show »
    Length:353
    Mass (Da):38,760
    Checksum:i982B08C9F2B9976B
    GO
    Isoform 3 (identifier: O14775-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-42: Missing.
         140-209: Missing.

    Show »
    Length:283
    Mass (Da):31,247
    Checksum:iBC7C4F15B805825B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti223 – 2231D → G in BAG37312. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti213 – 2131A → V.
    Corresponds to variant rs34637551 [ dbSNP | Ensembl ].
    VAR_049270

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 4242Missing in isoform 2 and isoform 3. 5 PublicationsVSP_008765Add
    BLAST
    Alternative sequencei140 – 20970Missing in isoform 3. 1 PublicationVSP_039101Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF017656 mRNA. Translation: AAC63826.1.
    AL117471 mRNA. Translation: CAB55946.1.
    AF501885 mRNA. Translation: AAM15921.1.
    AF300650 mRNA. Translation: AAG18444.1.
    AK314775 mRNA. Translation: BAG37312.1.
    BC013997 mRNA. Translation: AAH13997.1.
    CCDSiCCDS10149.1. [O14775-1]
    CCDS45261.1. [O14775-2]
    PIRiT17256.
    RefSeqiNP_006569.1. NM_006578.3. [O14775-2]
    NP_057278.2. NM_016194.3. [O14775-1]
    UniGeneiHs.155090.

    Genome annotation databases

    EnsembliENST00000261837; ENSP00000261837; ENSG00000069966. [O14775-1]
    ENST00000358784; ENSP00000351635; ENSG00000069966. [O14775-2]
    ENST00000396335; ENSP00000379626; ENSG00000069966. [O14775-3]
    GeneIDi10681.
    KEGGihsa:10681.
    UCSCiuc002abr.1. human. [O14775-1]
    uc002abs.1. human. [O14775-3]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF017656 mRNA. Translation: AAC63826.1 .
    AL117471 mRNA. Translation: CAB55946.1 .
    AF501885 mRNA. Translation: AAM15921.1 .
    AF300650 mRNA. Translation: AAG18444.1 .
    AK314775 mRNA. Translation: BAG37312.1 .
    BC013997 mRNA. Translation: AAH13997.1 .
    CCDSi CCDS10149.1. [O14775-1 ]
    CCDS45261.1. [O14775-2 ]
    PIRi T17256.
    RefSeqi NP_006569.1. NM_006578.3. [O14775-2 ]
    NP_057278.2. NM_016194.3. [O14775-1 ]
    UniGenei Hs.155090.

    3D structure databases

    ProteinModelPortali O14775.
    SMRi O14775. Positions 43-395.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115920. 26 interactions.
    IntActi O14775. 27 interactions.
    MINTi MINT-1399610.
    STRINGi 9606.ENSP00000261837.

    PTM databases

    PhosphoSitei O14775.

    Proteomic databases

    MaxQBi O14775.
    PaxDbi O14775.
    PRIDEi O14775.

    Protocols and materials databases

    DNASUi 10681.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000261837 ; ENSP00000261837 ; ENSG00000069966 . [O14775-1 ]
    ENST00000358784 ; ENSP00000351635 ; ENSG00000069966 . [O14775-2 ]
    ENST00000396335 ; ENSP00000379626 ; ENSG00000069966 . [O14775-3 ]
    GeneIDi 10681.
    KEGGi hsa:10681.
    UCSCi uc002abr.1. human. [O14775-1 ]
    uc002abs.1. human. [O14775-3 ]

    Organism-specific databases

    CTDi 10681.
    GeneCardsi GC15M052413.
    HGNCi HGNC:4401. GNB5.
    HPAi CAB032353.
    HPA041942.
    HPA044198.
    MIMi 604447. gene.
    neXtProti NX_O14775.
    PharmGKBi PA28780.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG2319.
    HOGENOMi HOG000176356.
    HOVERGENi HBG000188.
    InParanoidi O14775.
    KOi K04539.
    OMAi DQTFLVN.
    OrthoDBi EOG7GN2N5.
    PhylomeDBi O14775.
    TreeFami TF106149.

    Enzyme and pathway databases

    Reactomei REACT_15457. G-protein activation.
    REACT_163919. Inactivation, recovery and regulation of the phototransduction cascade.
    REACT_172761. Ca2+ pathway.
    REACT_18274. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
    REACT_18283. G alpha (q) signalling events.
    REACT_18339. Adrenaline,noradrenaline inhibits insulin secretion.
    REACT_18377. Glucagon-type ligand receptors.
    REACT_18407. G alpha (12/13) signalling events.
    REACT_19140. ADP signalling through P2Y purinoceptor 1.
    REACT_19145. G beta:gamma signalling through PLC beta.
    REACT_19231. G alpha (i) signalling events.
    REACT_19290. G beta:gamma signalling through PI3Kgamma.
    REACT_19327. G alpha (s) signalling events.
    REACT_19333. G alpha (z) signalling events.
    REACT_20647. Thromboxane signalling through TP receptor.
    REACT_20653. ADP signalling through P2Y purinoceptor 12.
    REACT_21254. Presynaptic function of Kainate receptors.
    REACT_21384. Thrombin signalling through proteinase activated receptors (PARs).
    REACT_23946. Prostacyclin signalling through prostacyclin receptor.
    REACT_24023. Regulation of water balance by renal Aquaporins.
    SignaLinki O14775.

    Miscellaneous databases

    ChiTaRSi GNB5. human.
    GeneWikii GNB5.
    GenomeRNAii 10681.
    NextBioi 35468110.
    PROi O14775.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O14775.
    Bgeei O14775.
    CleanExi HS_GNB5.
    Genevestigatori O14775.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR020472. G-protein_beta_WD-40_rep.
    IPR001632. Gprotein_B.
    IPR016346. Guanine_nucleotide-bd_bsu.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 7 hits.
    [Graphical view ]
    PIRSFi PIRSF002394. GN-bd_beta. 1 hit.
    PRINTSi PR00319. GPROTEINB.
    PR00320. GPROTEINBRPT.
    SMARTi SM00320. WD40. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 3 hits.
    PS50082. WD_REPEATS_2. 6 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and tissue distribution of the human G protein beta 5 cDNA."
      Jones P.G., Lombardi S.J., Cockett M.I.
      Biochim. Biophys. Acta 1402:288-291(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Tissue: Uterus.
    3. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Puhl H.L. III, Ikeda S.R., Aronstam R.S.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Cerebellum.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Lymph.

    Entry informationi

    Entry nameiGBB5_HUMAN
    AccessioniPrimary (citable) accession number: O14775
    Secondary accession number(s): B2RBR5, Q9HAU9, Q9UFT3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: October 31, 2003
    Last modified: October 1, 2014
    This is version 141 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3