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Protein

Guanine nucleotide-binding protein subunit beta-5

Gene

GNB5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.Curated

GO - Molecular functioni

  • chaperone binding Source: UniProtKB
  • G-protein gamma-subunit binding Source: UniProtKB
  • GTPase activator activity Source: UniProtKB
  • GTPase activity Source: UniProtKB
  • signal transducer activity Source: UniProtKB

GO - Biological processi

  • G-protein coupled receptor signaling pathway Source: Ensembl
  • negative regulation of voltage-gated calcium channel activity Source: UniProtKB
  • positive regulation of GTPase activity Source: UniProtKB
  • protein folding Source: Reactome
  • signal transduction Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Enzyme and pathway databases

BioCyciZFISH:ENSG00000069966-MONOMER.
ReactomeiR-HSA-202040. G-protein activation.
R-HSA-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-HSA-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-HSA-392170. ADP signalling through P2Y purinoceptor 12.
R-HSA-392451. G beta:gamma signalling through PI3Kgamma.
R-HSA-392851. Prostacyclin signalling through prostacyclin receptor.
R-HSA-400042. Adrenaline,noradrenaline inhibits insulin secretion.
R-HSA-4086398. Ca2+ pathway.
R-HSA-416476. G alpha (q) signalling events.
R-HSA-416482. G alpha (12/13) signalling events.
R-HSA-418217. G beta:gamma signalling through PLC beta.
R-HSA-418555. G alpha (s) signalling events.
R-HSA-418592. ADP signalling through P2Y purinoceptor 1.
R-HSA-418594. G alpha (i) signalling events.
R-HSA-418597. G alpha (z) signalling events.
R-HSA-420092. Glucagon-type ligand receptors.
R-HSA-428930. Thromboxane signalling through TP receptor.
R-HSA-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-HSA-456926. Thrombin signalling through proteinase activated receptors (PARs).
R-HSA-500657. Presynaptic function of Kainate receptors.
R-HSA-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.
SignaLinkiO14775.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit beta-5
Alternative name(s):
Gbeta5
Transducin beta chain 5
Gene namesi
Name:GNB5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:4401. GNB5.

Subcellular locationi

  • Membrane By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi10681.
OpenTargetsiENSG00000069966.
PharmGKBiPA28780.

Polymorphism and mutation databases

BioMutaiGNB5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001277051 – 395Guanine nucleotide-binding protein subunit beta-5Add BLAST395

Proteomic databases

EPDiO14775.
MaxQBiO14775.
PaxDbiO14775.
PeptideAtlasiO14775.
PRIDEiO14775.

PTM databases

iPTMnetiO14775.
PhosphoSitePlusiO14775.

Expressioni

Tissue specificityi

Expressed in multiple tissues.1 Publication

Gene expression databases

BgeeiENSG00000069966.
CleanExiHS_GNB5.
ExpressionAtlasiO14775. baseline and differential.
GenevisibleiO14775. HS.

Organism-specific databases

HPAiCAB032353.
HPA041942.
HPA044198.

Interactioni

Subunit structurei

Heterotrimeric G proteins are composed of 3 subunits; alpha, beta and gamma. Interacts with RGS9. Component of a complex composed of RGS9 (isoform RGS9-1), GNB5 and RGS9BP (By similarity). Interacts with RGS6 and RGS7 (PubMed:10521509, PubMed:10339615).By similarity2 Publications

GO - Molecular functioni

  • chaperone binding Source: UniProtKB
  • G-protein gamma-subunit binding Source: UniProtKB

Protein-protein interaction databases

BioGridi115920. 38 interactors.
IntActiO14775. 27 interactors.
MINTiMINT-1399610.
STRINGi9606.ENSP00000261837.

Structurei

3D structure databases

ProteinModelPortaliO14775.
SMRiO14775.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati103 – 142WD 1Add BLAST40
Repeati145 – 184WD 2Add BLAST40
Repeati193 – 234WD 3Add BLAST42
Repeati236 – 278WD 4Add BLAST43
Repeati279 – 318WD 5Add BLAST40
Repeati320 – 362WD 6Add BLAST43
Repeati365 – 394WD 7Add BLAST30

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410IU55. Eukaryota.
ENOG410YDDC. LUCA.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiO14775.
KOiK04539.
OMAiYCSTCAE.
OrthoDBiEOG091G0A7T.
PhylomeDBiO14775.
TreeFamiTF106149.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O14775-1) [UniParc]FASTAAdd to basket
Also known as: Long, Beta-5L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCDQTFLVNV FGSCDKCFKQ RALRPVFKKS QQLSYCSTCA EIMATEGLHE
60 70 80 90 100
NETLASLKSE AESLKGKLEE ERAKLHDVEL HQVAERVEAL GQFVMKTRRT
110 120 130 140 150
LKGHGNKVLC MDWCKDKRRI VSSSQDGKVI VWDSFTTNKE HAVTMPCTWV
160 170 180 190 200
MACAYAPSGC AIACGGLDNK CSVYPLTFDK NENMAAKKKS VAMHTNYLSA
210 220 230 240 250
CSFTNSDMQI LTASGDGTCA LWDVESGQLL QSFHGHGADV LCLDLAPSET
260 270 280 290 300
GNTFVSGGCD KKAMVWDMRS GQCVQAFETH ESDINSVRYY PSGDAFASGS
310 320 330 340 350
DDATCRLYDL RADREVAIYS KESIIFGASS VDFSLSGRLL FAGYNDYTIN
360 370 380 390
VWDVLKGSRV SILFGHENRV STLRVSPDGT AFCSGSWDHT LRVWA
Length:395
Mass (Da):43,566
Last modified:October 31, 2003 - v2
Checksum:iE001B07FCFA587AD
GO
Isoform 2 (identifier: O14775-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.

Show »
Length:353
Mass (Da):38,760
Checksum:i982B08C9F2B9976B
GO
Isoform 3 (identifier: O14775-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.
     140-209: Missing.

Show »
Length:283
Mass (Da):31,247
Checksum:iBC7C4F15B805825B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti223D → G in BAG37312 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049270213A → V.Corresponds to variant rs34637551dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0087651 – 42Missing in isoform 2 and isoform 3. 5 PublicationsAdd BLAST42
Alternative sequenceiVSP_039101140 – 209Missing in isoform 3. 1 PublicationAdd BLAST70

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017656 mRNA. Translation: AAC63826.1.
AL117471 mRNA. Translation: CAB55946.1.
AF501885 mRNA. Translation: AAM15921.1.
AF300650 mRNA. Translation: AAG18444.1.
AK314775 mRNA. Translation: BAG37312.1.
BC013997 mRNA. Translation: AAH13997.1.
CCDSiCCDS10149.1. [O14775-1]
CCDS45261.1. [O14775-2]
PIRiT17256.
RefSeqiNP_006569.1. NM_006578.3. [O14775-2]
NP_057278.2. NM_016194.3. [O14775-1]
UniGeneiHs.155090.

Genome annotation databases

EnsembliENST00000261837; ENSP00000261837; ENSG00000069966. [O14775-1]
ENST00000358784; ENSP00000351635; ENSG00000069966. [O14775-2]
ENST00000396335; ENSP00000379626; ENSG00000069966. [O14775-3]
GeneIDi10681.
KEGGihsa:10681.
UCSCiuc002abr.2. human. [O14775-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017656 mRNA. Translation: AAC63826.1.
AL117471 mRNA. Translation: CAB55946.1.
AF501885 mRNA. Translation: AAM15921.1.
AF300650 mRNA. Translation: AAG18444.1.
AK314775 mRNA. Translation: BAG37312.1.
BC013997 mRNA. Translation: AAH13997.1.
CCDSiCCDS10149.1. [O14775-1]
CCDS45261.1. [O14775-2]
PIRiT17256.
RefSeqiNP_006569.1. NM_006578.3. [O14775-2]
NP_057278.2. NM_016194.3. [O14775-1]
UniGeneiHs.155090.

3D structure databases

ProteinModelPortaliO14775.
SMRiO14775.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115920. 38 interactors.
IntActiO14775. 27 interactors.
MINTiMINT-1399610.
STRINGi9606.ENSP00000261837.

PTM databases

iPTMnetiO14775.
PhosphoSitePlusiO14775.

Polymorphism and mutation databases

BioMutaiGNB5.

Proteomic databases

EPDiO14775.
MaxQBiO14775.
PaxDbiO14775.
PeptideAtlasiO14775.
PRIDEiO14775.

Protocols and materials databases

DNASUi10681.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261837; ENSP00000261837; ENSG00000069966. [O14775-1]
ENST00000358784; ENSP00000351635; ENSG00000069966. [O14775-2]
ENST00000396335; ENSP00000379626; ENSG00000069966. [O14775-3]
GeneIDi10681.
KEGGihsa:10681.
UCSCiuc002abr.2. human. [O14775-1]

Organism-specific databases

CTDi10681.
DisGeNETi10681.
GeneCardsiGNB5.
HGNCiHGNC:4401. GNB5.
HPAiCAB032353.
HPA041942.
HPA044198.
MIMi604447. gene.
neXtProtiNX_O14775.
OpenTargetsiENSG00000069966.
PharmGKBiPA28780.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IU55. Eukaryota.
ENOG410YDDC. LUCA.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiO14775.
KOiK04539.
OMAiYCSTCAE.
OrthoDBiEOG091G0A7T.
PhylomeDBiO14775.
TreeFamiTF106149.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000069966-MONOMER.
ReactomeiR-HSA-202040. G-protein activation.
R-HSA-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-HSA-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-HSA-392170. ADP signalling through P2Y purinoceptor 12.
R-HSA-392451. G beta:gamma signalling through PI3Kgamma.
R-HSA-392851. Prostacyclin signalling through prostacyclin receptor.
R-HSA-400042. Adrenaline,noradrenaline inhibits insulin secretion.
R-HSA-4086398. Ca2+ pathway.
R-HSA-416476. G alpha (q) signalling events.
R-HSA-416482. G alpha (12/13) signalling events.
R-HSA-418217. G beta:gamma signalling through PLC beta.
R-HSA-418555. G alpha (s) signalling events.
R-HSA-418592. ADP signalling through P2Y purinoceptor 1.
R-HSA-418594. G alpha (i) signalling events.
R-HSA-418597. G alpha (z) signalling events.
R-HSA-420092. Glucagon-type ligand receptors.
R-HSA-428930. Thromboxane signalling through TP receptor.
R-HSA-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-HSA-456926. Thrombin signalling through proteinase activated receptors (PARs).
R-HSA-500657. Presynaptic function of Kainate receptors.
R-HSA-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.
SignaLinkiO14775.

Miscellaneous databases

ChiTaRSiGNB5. human.
GeneWikiiGNB5.
GenomeRNAii10681.
PROiO14775.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000069966.
CleanExiHS_GNB5.
ExpressionAtlasiO14775. baseline and differential.
GenevisibleiO14775. HS.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGBB5_HUMAN
AccessioniPrimary (citable) accession number: O14775
Secondary accession number(s): B2RBR5, Q9HAU9, Q9UFT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: October 31, 2003
Last modified: November 2, 2016
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.