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O14775 (GBB5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanine nucleotide-binding protein subunit beta-5
Alternative name(s):
Gbeta5
Transducin beta chain 5
Gene names
Name:GNB5
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length395 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Subunit structure

G proteins are composed of 3 units, alpha, beta and gamma. Component of the RGS9-1-Gbeta5 complex composed of RGS9 (isoform RGS9-1) Gbeta5 (GNB5) and RGS9BP By similarity.

Tissue specificity

Expressed in multiple tissues.

Sequence similarities

Belongs to the WD repeat G protein beta family.

Contains 7 WD repeats.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O14775-1)

Also known as: Long; Beta-5L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O14775-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.
Isoform 3 (identifier: O14775-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.
     140-209: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 395395Guanine nucleotide-binding protein subunit beta-5
PRO_0000127705

Regions

Repeat103 – 14240WD 1
Repeat145 – 18440WD 2
Repeat193 – 23442WD 3
Repeat236 – 27843WD 4
Repeat279 – 31840WD 5
Repeat320 – 36243WD 6
Repeat365 – 39430WD 7

Natural variations

Alternative sequence1 – 4242Missing in isoform 2 and isoform 3.
VSP_008765
Alternative sequence140 – 20970Missing in isoform 3.
VSP_039101
Natural variant2131A → V.
Corresponds to variant rs34637551 [ dbSNP | Ensembl ].
VAR_049270

Experimental info

Sequence conflict2231D → G in BAG37312. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Long) (Beta-5L) [UniParc].

Last modified October 31, 2003. Version 2.
Checksum: E001B07FCFA587AD

FASTA39543,566
        10         20         30         40         50         60 
MCDQTFLVNV FGSCDKCFKQ RALRPVFKKS QQLSYCSTCA EIMATEGLHE NETLASLKSE 

        70         80         90        100        110        120 
AESLKGKLEE ERAKLHDVEL HQVAERVEAL GQFVMKTRRT LKGHGNKVLC MDWCKDKRRI 

       130        140        150        160        170        180 
VSSSQDGKVI VWDSFTTNKE HAVTMPCTWV MACAYAPSGC AIACGGLDNK CSVYPLTFDK 

       190        200        210        220        230        240 
NENMAAKKKS VAMHTNYLSA CSFTNSDMQI LTASGDGTCA LWDVESGQLL QSFHGHGADV 

       250        260        270        280        290        300 
LCLDLAPSET GNTFVSGGCD KKAMVWDMRS GQCVQAFETH ESDINSVRYY PSGDAFASGS 

       310        320        330        340        350        360 
DDATCRLYDL RADREVAIYS KESIIFGASS VDFSLSGRLL FAGYNDYTIN VWDVLKGSRV 

       370        380        390 
SILFGHENRV STLRVSPDGT AFCSGSWDHT LRVWA 

« Hide

Isoform 2 [UniParc].

Checksum: 982B08C9F2B9976B
Show »

FASTA35338,760
Isoform 3 [UniParc].

Checksum: BC7C4F15B805825B
Show »

FASTA28331,247

References

« Hide 'large scale' references
[1]"Cloning and tissue distribution of the human G protein beta 5 cDNA."
Jones P.G., Lombardi S.J., Cockett M.I.
Biochim. Biophys. Acta 1402:288-291(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs."
Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. expand/collapse author list , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A.
Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Uterus.
[3]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Cerebellum.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Lymph.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF017656 mRNA. Translation: AAC63826.1.
AL117471 mRNA. Translation: CAB55946.1.
AF501885 mRNA. Translation: AAM15921.1.
AF300650 mRNA. Translation: AAG18444.1.
AK314775 mRNA. Translation: BAG37312.1.
BC013997 mRNA. Translation: AAH13997.1.
CCDSCCDS10149.1. [O14775-1]
CCDS45261.1. [O14775-2]
PIRT17256.
RefSeqNP_006569.1. NM_006578.3. [O14775-2]
NP_057278.2. NM_016194.3. [O14775-1]
UniGeneHs.155090.

3D structure databases

ProteinModelPortalO14775.
SMRO14775. Positions 43-395.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115920. 26 interactions.
IntActO14775. 14 interactions.
MINTMINT-1399610.
STRING9606.ENSP00000261837.

PTM databases

PhosphoSiteO14775.

Proteomic databases

MaxQBO14775.
PaxDbO14775.
PRIDEO14775.

Protocols and materials databases

DNASU10681.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000261837; ENSP00000261837; ENSG00000069966. [O14775-1]
ENST00000358784; ENSP00000351635; ENSG00000069966. [O14775-2]
ENST00000396335; ENSP00000379626; ENSG00000069966. [O14775-3]
GeneID10681.
KEGGhsa:10681.
UCSCuc002abr.1. human. [O14775-1]
uc002abs.1. human. [O14775-3]

Organism-specific databases

CTD10681.
GeneCardsGC15M052413.
HGNCHGNC:4401. GNB5.
HPACAB032353.
HPA041942.
HPA044198.
MIM604447. gene.
neXtProtNX_O14775.
PharmGKBPA28780.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2319.
HOGENOMHOG000176356.
HOVERGENHBG000188.
InParanoidO14775.
KOK04539.
OMADQTFLVN.
OrthoDBEOG7GN2N5.
PhylomeDBO14775.
TreeFamTF106149.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_111217. Metabolism.
REACT_116125. Disease.
REACT_13685. Neuronal System.
REACT_15518. Transmembrane transport of small molecules.
REACT_604. Hemostasis.
SignaLinkO14775.

Gene expression databases

ArrayExpressO14775.
BgeeO14775.
CleanExHS_GNB5.
GenevestigatorO14775.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 7 hits.
[Graphical view]
PIRSFPIRSF002394. GN-bd_beta. 1 hit.
PRINTSPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGNB5. human.
GeneWikiGNB5.
GenomeRNAi10681.
NextBio35468110.
PROO14775.
SOURCESearch...

Entry information

Entry nameGBB5_HUMAN
AccessionPrimary (citable) accession number: O14775
Secondary accession number(s): B2RBR5, Q9HAU9, Q9UFT3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: October 31, 2003
Last modified: July 9, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM