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O14735 (CDIPT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CDP-diacylglycerol--inositol 3-phosphatidyltransferase

EC=2.7.8.11
Alternative name(s):
Phosphatidylinositol synthase
Short name=PI synthase
Short name=PtdIns synthase
Gene names
Name:CDIPT
Synonyms:PIS, PIS1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length213 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the biosynthesis of phosphatidylinositol (PtdIns) as well as PtdIns:inositol exchange reaction. May thus act to reduce an excessive cellular PtdIns content. The exchange activity is due to the reverse reaction of PtdIns synthase and is dependent on CMP, which is tightly bound to the enzyme. Ref.6

Catalytic activity

CDP-diacylglycerol + myo-inositol = CMP + phosphatidyl-1D-myo-inositol. Ref.6

Cofactor

Divalent metal cations; Mn2+ or Mg2+. Ref.6

Enzyme regulation

Inhibited by PtdIns (product inhibition), phosphatidylinositol phosphate, and nucleoside di- and tri-phosphates.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity. Golgi apparatus membrane; Multi-pass membrane protein By similarity. Cell membrane; Multi-pass membrane protein By similarity. Membrane Ref.6.

Tissue specificity

Detected in placenta (at protein level). Widely expressed. Higher expression in adult liver and skeletal muscle, slightly lower levels seen in pancreas, kidney, lung, placenta, brain, heart, leukocyte, colon, small intestine, ovary, testis, prostate, thymus and spleen. In fetus, expressed in kidney, liver, lung and brain. Ref.6

Sequence similarities

Belongs to the CDP-alcohol phosphatidyltransferase class-I family.

Biophysicochemical properties

pH dependence:

Optimum pH is 9.0. Ref.6

Temperature dependence:

Optimum temperature is 50 degrees Celsius.

Ontologies

Keywords
   Biological processLipid biosynthesis
Lipid metabolism
Phospholipid biosynthesis
Phospholipid metabolism
   Cellular componentCell membrane
Endoplasmic reticulum
Golgi apparatus
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainTransmembrane
Transmembrane helix
   LigandMagnesium
Manganese
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processCDP-diacylglycerol metabolic process

Inferred from electronic annotation. Source: Ensembl

glycerophospholipid biosynthetic process

Traceable author statement. Source: Reactome

phosphatidylinositol biosynthetic process

Inferred from direct assay Ref.6Ref.1. Source: UniProtKB

phospholipid metabolic process

Traceable author statement. Source: Reactome

small molecule metabolic process

Traceable author statement. Source: Reactome

   Cellular_componentGolgi membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

endoplasmic reticulum membrane

Traceable author statement. Source: Reactome

integral component of membrane

Inferred from direct assay Ref.6. Source: UniProtKB

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionCDP-diacylglycerol-inositol 3-phosphatidyltransferase activity

Inferred from direct assay Ref.6Ref.1. Source: UniProtKB

alcohol binding

Inferred from electronic annotation. Source: Ensembl

carbohydrate binding

Inferred from electronic annotation. Source: Ensembl

diacylglycerol binding

Inferred from electronic annotation. Source: Ensembl

manganese ion binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O14735-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O14735-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-14: MPDENIFLFVPNLI → MLPTAAGFSI...PALSPRVPFQ
     111-129: SSVVRGSESHKMIDLSGNP → RSAAILGAWATWRHYSGVG
     130-213: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 213213CDP-diacylglycerol--inositol 3-phosphatidyltransferase
PRO_0000056802

Regions

Transmembrane6 – 2621Helical; Potential
Transmembrane29 – 4921Helical; Potential
Transmembrane76 – 9621Helical; Potential
Transmembrane140 – 16021Helical; Potential
Transmembrane175 – 19521Helical; Potential

Natural variations

Alternative sequence1 – 1414MPDEN…VPNLI → MLPTAAGFSIWGQVGAAREA PRCQTKISSCSCPTSSVSAA HGPGPNERARGLGGLPDPAL SPRVPFQ in isoform 2.
VSP_013618
Alternative sequence111 – 12919SSVVR…LSGNP → RSAAILGAWATWRHYSGVG in isoform 2.
VSP_013619
Alternative sequence130 – 21384Missing in isoform 2.
VSP_013620
Natural variant1991R → C.
Corresponds to variant rs1802002 [ dbSNP | Ensembl ].
VAR_048734

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 7885B72EF2A88093

FASTA21323,539
        10         20         30         40         50         60 
MPDENIFLFV PNLIGYARIV FAIISFYFMP CCPLTASSFY LLSGLLDAFD GHAARALNQG 

        70         80         90        100        110        120 
TRFGAMLDML TDRCSTMCLL VNLALLYPGA TLFFQISMSL DVASHWLHLH SSVVRGSESH 

       130        140        150        160        170        180 
KMIDLSGNPV LRIYYTSRPA LFTLCAGNEL FYCLLYLFHF SEGPLVGSVG LFRMGLWVTA 

       190        200        210 
PIALLKSLIS VIHLITAARN MAALDAADRA KKK 

« Hide

Isoform 2 [UniParc].

Checksum: 390F56D184377985
Show »

FASTA18219,455

References

« Hide 'large scale' references
[1]"The role of CDP-diacylglycerol synthetase and phosphatidylinositol synthase activity levels in the regulation of cellular phosphatidylinositol content."
Lykidis A., Jackson P.D., Rock C.O., Jackowski S.
J. Biol. Chem. 272:33402-33409(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Testis.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Thymus.
[3]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[6]"Purification and characterization of phosphatidylinositol synthase from human placenta."
Antonsson B.E.
Biochem. J. 297:517-522(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Placenta.
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF014807 mRNA. Translation: AAB94860.1.
AK131349 mRNA. Translation: BAD18505.1.
BT007301 mRNA. Translation: AAP35965.1.
CR542016 mRNA. Translation: CAG46813.1.
BC001444 mRNA. Translation: AAH01444.1.
RefSeqNP_006310.1. NM_006319.4.
UniGeneHs.121549.

3D structure databases

ProteinModelPortalO14735.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115692. 12 interactions.
IntActO14735. 10 interactions.
MINTMINT-1150927.
STRING9606.ENSP00000219789.

PTM databases

PhosphoSiteO14735.

Proteomic databases

PaxDbO14735.
PRIDEO14735.

Protocols and materials databases

DNASU10423.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000219789; ENSP00000219789; ENSG00000103502. [O14735-1]
ENST00000569956; ENSP00000457339; ENSG00000103502. [O14735-1]
ENST00000570016; ENSP00000454453; ENSG00000103502. [O14735-1]
GeneID10423.
KEGGhsa:10423.
UCSCuc002dul.3. human. [O14735-1]

Organism-specific databases

CTD10423.
GeneCardsGC16M029869.
HGNCHGNC:1769. CDIPT.
HPAHPA045185.
MIM605893. gene.
neXtProtNX_O14735.
PharmGKBPA26306.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0558.
HOGENOMHOG000204051.
HOVERGENHBG050862.
InParanoidO14735.
KOK00999.
OMAIDVACHW.
PhylomeDBO14735.
TreeFamTF314603.

Enzyme and pathway databases

BioCycMetaCyc:HS02513-MONOMER.
ReactomeREACT_111217. Metabolism.

Gene expression databases

ArrayExpressO14735.
BgeeO14735.
CleanExHS_CDIPT.
GenevestigatorO14735.

Family and domain databases

InterProIPR000462. CDP-OH_P_trans.
IPR014387. CDP_diag_ino_3_P_euk.
[Graphical view]
PfamPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFPIRSF000848. CDP_diag_ino_3_P. 1 hit.
PROSITEPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiCDIPT.
GenomeRNAi10423.
NextBio39504.
PROO14735.
SOURCESearch...

Entry information

Entry nameCDIPT_HUMAN
AccessionPrimary (citable) accession number: O14735
Secondary accession number(s): Q6FGU1, Q6ZN70
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM