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Protein

CDP-diacylglycerol--inositol 3-phosphatidyltransferase

Gene

CDIPT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the biosynthesis of phosphatidylinositol (PtdIns) as well as PtdIns:inositol exchange reaction. May thus act to reduce an excessive cellular PtdIns content. The exchange activity is due to the reverse reaction of PtdIns synthase and is dependent on CMP, which is tightly bound to the enzyme.1 Publication

Catalytic activityi

CDP-diacylglycerol + myo-inositol = CMP + phosphatidyl-1D-myo-inositol.1 Publication

Cofactori

Mn2+1 Publication, Mg2+1 PublicationNote: Divalent metal cations; Mn(2+) or Mg2+.1 Publication

Enzyme regulationi

Inhibited by PtdIns (product inhibition), phosphatidylinositol phosphate, and nucleoside di- and tri-phosphates.

pH dependencei

Optimum pH is 9.0.1 Publication

Temperature dependencei

Optimum temperature is 50 degrees Celsius.1 Publication

GO - Molecular functioni

  1. alcohol binding Source: Ensembl
  2. carbohydrate binding Source: Ensembl
  3. CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity Source: UniProtKB
  4. diacylglycerol binding Source: Ensembl
  5. manganese ion binding Source: Ensembl

GO - Biological processi

  1. CDP-diacylglycerol metabolic process Source: Ensembl
  2. glycerophospholipid biosynthetic process Source: Reactome
  3. phosphatidylinositol biosynthetic process Source: UniProtKB
  4. phospholipid metabolic process Source: Reactome
  5. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

Magnesium, Manganese

Enzyme and pathway databases

BioCyciMetaCyc:HS02513-MONOMER.
ReactomeiREACT_121000. Synthesis of PI.

Names & Taxonomyi

Protein namesi
Recommended name:
CDP-diacylglycerol--inositol 3-phosphatidyltransferase (EC:2.7.8.11)
Alternative name(s):
Phosphatidylinositol synthase
Short name:
PI synthase
Short name:
PtdIns synthase
Gene namesi
Name:CDIPT
Synonyms:PIS, PIS1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 16

Organism-specific databases

HGNCiHGNC:1769. CDIPT.

Subcellular locationi

Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity. Golgi apparatus membrane By similarity; Multi-pass membrane protein By similarity. Cell membrane By similarity; Multi-pass membrane protein By similarity. Membrane 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei6 – 2621HelicalSequence AnalysisAdd
BLAST
Transmembranei29 – 4921HelicalSequence AnalysisAdd
BLAST
Transmembranei76 – 9621HelicalSequence AnalysisAdd
BLAST
Transmembranei140 – 16021HelicalSequence AnalysisAdd
BLAST
Transmembranei175 – 19521HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: Reactome
  2. Golgi apparatus Source: GO_Central
  3. Golgi membrane Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB
  5. membrane Source: UniProtKB
  6. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26306.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 213213CDP-diacylglycerol--inositol 3-phosphatidyltransferasePRO_0000056802Add
BLAST

Proteomic databases

MaxQBiO14735.
PaxDbiO14735.
PRIDEiO14735.

PTM databases

PhosphoSiteiO14735.

Expressioni

Tissue specificityi

Detected in placenta (at protein level). Widely expressed. Higher expression in adult liver and skeletal muscle, slightly lower levels seen in pancreas, kidney, lung, placenta, brain, heart, leukocyte, colon, small intestine, ovary, testis, prostate, thymus and spleen. In fetus, expressed in kidney, liver, lung and brain.1 Publication

Gene expression databases

BgeeiO14735.
CleanExiHS_CDIPT.
ExpressionAtlasiO14735. baseline and differential.
GenevestigatoriO14735.

Organism-specific databases

HPAiHPA045185.
HPA056597.

Interactioni

Protein-protein interaction databases

BioGridi115692. 16 interactions.
IntActiO14735. 10 interactions.
MINTiMINT-1150927.
STRINGi9606.ENSP00000219789.

Structurei

3D structure databases

ProteinModelPortaliO14735.
SMRiO14735. Positions 10-103.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0558.
GeneTreeiENSGT00390000017279.
HOGENOMiHOG000204051.
HOVERGENiHBG050862.
InParanoidiO14735.
KOiK00999.
OMAiTDRCGTM.
PhylomeDBiO14735.
TreeFamiTF314603.

Family and domain databases

InterProiIPR000462. CDP-OH_P_trans.
IPR014387. CDP_diag_ino_3_P_euk.
[Graphical view]
PfamiPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF000848. CDP_diag_ino_3_P. 1 hit.
PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O14735-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPDENIFLFV PNLIGYARIV FAIISFYFMP CCPLTASSFY LLSGLLDAFD
60 70 80 90 100
GHAARALNQG TRFGAMLDML TDRCSTMCLL VNLALLYPGA TLFFQISMSL
110 120 130 140 150
DVASHWLHLH SSVVRGSESH KMIDLSGNPV LRIYYTSRPA LFTLCAGNEL
160 170 180 190 200
FYCLLYLFHF SEGPLVGSVG LFRMGLWVTA PIALLKSLIS VIHLITAARN
210
MAALDAADRA KKK
Length:213
Mass (Da):23,539
Last modified:January 1, 1998 - v1
Checksum:i7885B72EF2A88093
GO
Isoform 2 (identifier: O14735-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-14: MPDENIFLFVPNLI → MLPTAAGFSI...PALSPRVPFQ
     111-129: SSVVRGSESHKMIDLSGNP → RSAAILGAWATWRHYSGVG
     130-213: Missing.

Show »
Length:182
Mass (Da):19,455
Checksum:i390F56D184377985
GO
Isoform 3 (identifier: O14735-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     15-59: Missing.

Show »
Length:168
Mass (Da):18,624
Checksum:i7941BB498981C00C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti75 – 751S → P in BAG62462 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti199 – 1991R → C.
Corresponds to variant rs1802002 [ dbSNP | Ensembl ].
VAR_048734

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1414MPDEN…VPNLI → MLPTAAGFSIWGQVGAAREA PRCQTKISSCSCPTSSVSAA HGPGPNERARGLGGLPDPAL SPRVPFQ in isoform 2. 1 PublicationVSP_013618Add
BLAST
Alternative sequencei15 – 5945Missing in isoform 3. 1 PublicationVSP_054767Add
BLAST
Alternative sequencei111 – 12919SSVVR…LSGNP → RSAAILGAWATWRHYSGVG in isoform 2. 1 PublicationVSP_013619Add
BLAST
Alternative sequencei130 – 21384Missing in isoform 2. 1 PublicationVSP_013620Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014807 mRNA. Translation: AAB94860.1.
BT007301 mRNA. Translation: AAP35965.1.
AK131349 mRNA. Translation: BAD18505.1.
AK300805 mRNA. Translation: BAG62462.1.
CR542016 mRNA. Translation: CAG46813.1.
AC120114 Genomic DNA. No translation available.
BC001444 mRNA. Translation: AAH01444.1.
CCDSiCCDS10657.1. [O14735-1]
CCDS67002.1. [O14735-3]
RefSeqiNP_001273514.1. NM_001286585.1. [O14735-3]
NP_006310.1. NM_006319.4. [O14735-1]
UniGeneiHs.121549.

Genome annotation databases

EnsembliENST00000219789; ENSP00000219789; ENSG00000103502. [O14735-1]
ENST00000566113; ENSP00000457340; ENSG00000103502. [O14735-3]
ENST00000569956; ENSP00000457339; ENSG00000103502. [O14735-1]
ENST00000570016; ENSP00000454453; ENSG00000103502. [O14735-1]
GeneIDi10423.
KEGGihsa:10423.
UCSCiuc002dul.3. human. [O14735-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014807 mRNA. Translation: AAB94860.1.
BT007301 mRNA. Translation: AAP35965.1.
AK131349 mRNA. Translation: BAD18505.1.
AK300805 mRNA. Translation: BAG62462.1.
CR542016 mRNA. Translation: CAG46813.1.
AC120114 Genomic DNA. No translation available.
BC001444 mRNA. Translation: AAH01444.1.
CCDSiCCDS10657.1. [O14735-1]
CCDS67002.1. [O14735-3]
RefSeqiNP_001273514.1. NM_001286585.1. [O14735-3]
NP_006310.1. NM_006319.4. [O14735-1]
UniGeneiHs.121549.

3D structure databases

ProteinModelPortaliO14735.
SMRiO14735. Positions 10-103.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115692. 16 interactions.
IntActiO14735. 10 interactions.
MINTiMINT-1150927.
STRINGi9606.ENSP00000219789.

PTM databases

PhosphoSiteiO14735.

Proteomic databases

MaxQBiO14735.
PaxDbiO14735.
PRIDEiO14735.

Protocols and materials databases

DNASUi10423.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000219789; ENSP00000219789; ENSG00000103502. [O14735-1]
ENST00000566113; ENSP00000457340; ENSG00000103502. [O14735-3]
ENST00000569956; ENSP00000457339; ENSG00000103502. [O14735-1]
ENST00000570016; ENSP00000454453; ENSG00000103502. [O14735-1]
GeneIDi10423.
KEGGihsa:10423.
UCSCiuc002dul.3. human. [O14735-1]

Organism-specific databases

CTDi10423.
GeneCardsiGC16M029869.
HGNCiHGNC:1769. CDIPT.
HPAiHPA045185.
HPA056597.
MIMi605893. gene.
neXtProtiNX_O14735.
PharmGKBiPA26306.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0558.
GeneTreeiENSGT00390000017279.
HOGENOMiHOG000204051.
HOVERGENiHBG050862.
InParanoidiO14735.
KOiK00999.
OMAiTDRCGTM.
PhylomeDBiO14735.
TreeFamiTF314603.

Enzyme and pathway databases

BioCyciMetaCyc:HS02513-MONOMER.
ReactomeiREACT_121000. Synthesis of PI.

Miscellaneous databases

GeneWikiiCDIPT.
GenomeRNAii10423.
NextBioi35475278.
PROiO14735.
SOURCEiSearch...

Gene expression databases

BgeeiO14735.
CleanExiHS_CDIPT.
ExpressionAtlasiO14735. baseline and differential.
GenevestigatoriO14735.

Family and domain databases

InterProiIPR000462. CDP-OH_P_trans.
IPR014387. CDP_diag_ino_3_P_euk.
[Graphical view]
PfamiPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF000848. CDP_diag_ino_3_P. 1 hit.
PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The role of CDP-diacylglycerol synthetase and phosphatidylinositol synthase activity levels in the regulation of cellular phosphatidylinositol content."
    Lykidis A., Jackson P.D., Rock C.O., Jackowski S.
    J. Biol. Chem. 272:33402-33409(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Testis.
  2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    Tissue: Thymus.
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "The sequence and analysis of duplication-rich human chromosome 16."
    Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
    , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
    Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  7. "Purification and characterization of phosphatidylinositol synthase from human placenta."
    Antonsson B.E.
    Biochem. J. 297:517-522(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Placenta.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCDIPT_HUMAN
AccessioniPrimary (citable) accession number: O14735
Secondary accession number(s): B4DUV0
, H3BTV1, Q6FGU1, Q6ZN70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: March 4, 2015
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.