O14735 (CDIPT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CDP-diacylglycerol--inositol 3-phosphatidyltransferase
EC=2.7.8.11

Alternative name(s):
Phosphatidylinositol synthase
Short name=PI synthase
Short name=PtdIns synthase

Gene names

Name:	CDIPT
Synonyms:	PIS, PIS1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the biosynthesis of phosphatidylinositol (PtdIns) as well as PtdIns:inositol exchange reaction. May thus act to reduce an excessive cellular PtdIns content. The exchange activity is due to the reverse reaction of PtdIns synthase and is dependent on CMP, which is tightly bound to the enzyme.
Catalytic activity	CDP-diacylglycerol + myo-inositol = CMP + phosphatidyl-1D-myo-inositol.
Cofactor	Magnesium. Manganese.
Enzyme regulation	Inhibited by PtdIns (product inhibition), phosphatidylinositol phosphate, and nucleoside di- and tri-phosphates.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity. Golgi apparatus membrane; Multi-pass membrane protein By similarity. Cell membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Widely expressed. Higher expression in adult liver and skeletal muscle, slightly lower levels seen in pancreas, kidney, lung, placenta, brain, heart, leukocyte, colon, small intestine, ovary, testis, prostate, thymus and spleen. In fetus, expressed in kidney, liver, lung and brain.
Sequence similarities	Belongs to the CDP-alcohol phosphatidyltransferase class-I family.
Biophysicochemical properties	pH dependence: Optimum pH is 9.0. Temperature dependence: Optimum temperature is 50 degrees Celsius.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Phospholipid biosynthesis Phospholipid metabolism
Cellular component	Cell membrane Endoplasmic reticulum Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium Manganese
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CDP-diacylglycerol metabolic process Inferred from electronic annotation. Source: Compara phosphatidylinositol biosynthetic process Inferred from direct assay Ref.1. Source: UniProtKB small molecule metabolic process Traceable author statement. Source: Reactome
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Traceable author statement. Source: Reactome integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity Inferred from direct assay Ref.1. Source: UniProtKB alcohol binding Inferred from electronic annotation. Source: Compara carbohydrate binding Inferred from electronic annotation. Source: Compara diacylglycerol binding Inferred from electronic annotation. Source: Compara manganese ion binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O14735-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O14735-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-14: MPDENIFLFVPNLI → MLPTAAGFSI...PALSPRVPFQ
     111-129: SSVVRGSESHKMIDLSGNP → RSAAILGAWATWRHYSGVG
     130-213: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	CDP-diacylglycerol--inositol 3-phosphatidyltransferase		PRO_0000056802
Regions
	Transmembrane	6 – 26	21	Helical; Potential
	Transmembrane	29 – 49	21	Helical; Potential
	Transmembrane	76 – 96	21	Helical; Potential
	Transmembrane	140 – 160	21	Helical; Potential
	Transmembrane	175 – 195	21	Helical; Potential
Natural variations
	Alternative sequence	1 – 14	14	MPDEN…VPNLI → MLPTAAGFSIWGQVGAAREA PRCQTKISSCSCPTSSVSAA HGPGPNERARGLGGLPDPAL SPRVPFQ in isoform 2.		VSP_013618
	Alternative sequence	111 – 129	19	SSVVR…LSGNP → RSAAILGAWATWRHYSGVG in isoform 2.		VSP_013619
	Alternative sequence	130 – 213	84	Missing in isoform 2.		VSP_013620
	Natural variant	199	1	R → C. Corresponds to variant rs1802002 [ dbSNP | Ensembl ].		VAR_048734

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 7885B72EF2A88093	FASTA	213	23,539
	10 20 30 40 50 60 MPDENIFLFV PNLIGYARIV FAIISFYFMP CCPLTASSFY LLSGLLDAFD GHAARALNQG 70 80 90 100 110 120 TRFGAMLDML TDRCSTMCLL VNLALLYPGA TLFFQISMSL DVASHWLHLH SSVVRGSESH 130 140 150 160 170 180 KMIDLSGNPV LRIYYTSRPA LFTLCAGNEL FYCLLYLFHF SEGPLVGSVG LFRMGLWVTA 190 200 210 PIALLKSLIS VIHLITAARN MAALDAADRA KKK
	Isoform 2 [UniParc]. Checksum: 390F56D184377985	FASTA	182	19,455
	10 20 30 40 50 60 MLPTAAGFSI WGQVGAAREA PRCQTKISSC SCPTSSVSAA HGPGPNERAR GLGGLPDPAL 70 80 90 100 110 120 SPRVPFQGYA RIVFAIISFY FMPCCPLTAS SFYLLSGLLD AFDGHAARAL NQGTRFGAML 130 140 150 160 170 180 DMLTDRCSTM CLLVNLALLY PGATLFFQIS MSLDVASHWL HLHRSAAILG AWATWRHYSG VG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The role of CDP-diacylglycerol synthetase and phosphatidylinositol synthase activity levels in the regulation of cellular phosphatidylinositol content." Lykidis A., Jackson P.D., Rock C.O., Jackowski S. J. Biol. Chem. 272:33402-33409(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Testis.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Thymus.
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[6]	"Purification and characterization of phosphatidylinositol synthase from human placenta." Antonsson B.E. Biochem. J. 297:517-522(1994) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Tissue: Placenta.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF014807 mRNA. Translation: AAB94860.1. AK131349 mRNA. Translation: BAD18505.1. BT007301 mRNA. Translation: AAP35965.1. CR542016 mRNA. Translation: CAG46813.1. BC001444 mRNA. Translation: AAH01444.1.
IPI	IPI00442183. IPI00645518.
RefSeq	NP_006310.1. NM_006319.3.
UniGene	Hs.121549.
3D structure databases
ProteinModelPortal	O14735.
ModBase	Search...
Protein-protein interaction databases
IntAct	O14735. 7 interactions.
MINT	MINT-1150927.
STRING	9606.ENSP00000219789.
PTM databases
PhosphoSite	O14735.
Proteomic databases
PaxDb	O14735.
PRIDE	O14735.
Protocols and materials databases
DNASU	10423.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000219789; ENSP00000219789; ENSG00000103502. ENST00000569956; ENSP00000457339; ENSG00000103502. ENST00000570016; ENSP00000454453; ENSG00000103502.
GeneID	10423.
KEGG	hsa:10423.
UCSC	uc002dul.3. human.
Organism-specific databases
CTD	10423.
GeneCards	GC16M029869.
HGNC	HGNC:1769. CDIPT.
HPA	HPA045185.
MIM	605893. gene.
neXtProt	NX_O14735.
PharmGKB	PA26306.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0558.
HOGENOM	HOG000204051.
HOVERGEN	HBG050862.
InParanoid	O14735.
KO	K00999.
OMA	IDVACHW.
OrthoDB	EOG4PVNZT.
Enzyme and pathway databases
BioCyc	MetaCyc:HS02513-MONOMER.
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	O14735.
Bgee	O14735.
CleanEx	HS_CDIPT.
Genevestigator	O14735.
GermOnline	ENSG00000103502. Homo sapiens.
Family and domain databases
InterPro	IPR000462. CDP-OH_P_trans. IPR014387. CDP_diag_ino_3_P_euk. [Graphical view]
Pfam	PF01066. CDP-OH_P_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000848. CDP_diag_ino_3_P. 1 hit.
PROSITE	PS00379. CDP_ALCOHOL_P_TRANSF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	10423.
NextBio	39504.
SOURCE	Search...

Entry information

Entry name

CDIPT_HUMAN

Accession

Primary (citable) accession number: O14735
Secondary accession number(s): Q6FGU1, Q6ZN70

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.