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O14735

- CDIPT_HUMAN

UniProt

O14735 - CDIPT_HUMAN

Protein

CDP-diacylglycerol--inositol 3-phosphatidyltransferase

Gene

CDIPT

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the biosynthesis of phosphatidylinositol (PtdIns) as well as PtdIns:inositol exchange reaction. May thus act to reduce an excessive cellular PtdIns content. The exchange activity is due to the reverse reaction of PtdIns synthase and is dependent on CMP, which is tightly bound to the enzyme.1 Publication

    Catalytic activityi

    CDP-diacylglycerol + myo-inositol = CMP + phosphatidyl-1D-myo-inositol.1 Publication

    Cofactori

    Divalent metal cations; Mn2+ or Mg2+.1 Publication

    Enzyme regulationi

    Inhibited by PtdIns (product inhibition), phosphatidylinositol phosphate, and nucleoside di- and tri-phosphates.

    pH dependencei

    Optimum pH is 9.0.1 Publication

    Temperature dependencei

    Optimum temperature is 50 degrees Celsius.1 Publication

    GO - Molecular functioni

    1. alcohol binding Source: Ensembl
    2. carbohydrate binding Source: Ensembl
    3. CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity Source: UniProtKB
    4. diacylglycerol binding Source: Ensembl
    5. manganese ion binding Source: Ensembl

    GO - Biological processi

    1. CDP-diacylglycerol metabolic process Source: Ensembl
    2. glycerophospholipid biosynthetic process Source: Reactome
    3. phosphatidylinositol biosynthetic process Source: UniProtKB
    4. phospholipid metabolic process Source: Reactome
    5. small molecule metabolic process Source: Reactome

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Keywords - Ligandi

    Magnesium, Manganese

    Enzyme and pathway databases

    BioCyciMetaCyc:HS02513-MONOMER.
    ReactomeiREACT_121000. Synthesis of PI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CDP-diacylglycerol--inositol 3-phosphatidyltransferase (EC:2.7.8.11)
    Alternative name(s):
    Phosphatidylinositol synthase
    Short name:
    PI synthase
    Short name:
    PtdIns synthase
    Gene namesi
    Name:CDIPT
    Synonyms:PIS, PIS1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:1769. CDIPT.

    Subcellular locationi

    Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity. Golgi apparatus membrane By similarity; Multi-pass membrane protein By similarity. Cell membrane By similarity; Multi-pass membrane protein By similarity. Membrane 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: Reactome
    2. Golgi membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB
    4. membrane Source: UniProtKB
    5. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Endoplasmic reticulum, Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26306.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 213213CDP-diacylglycerol--inositol 3-phosphatidyltransferasePRO_0000056802Add
    BLAST

    Proteomic databases

    MaxQBiO14735.
    PaxDbiO14735.
    PRIDEiO14735.

    PTM databases

    PhosphoSiteiO14735.

    Expressioni

    Tissue specificityi

    Detected in placenta (at protein level). Widely expressed. Higher expression in adult liver and skeletal muscle, slightly lower levels seen in pancreas, kidney, lung, placenta, brain, heart, leukocyte, colon, small intestine, ovary, testis, prostate, thymus and spleen. In fetus, expressed in kidney, liver, lung and brain.1 Publication

    Gene expression databases

    ArrayExpressiO14735.
    BgeeiO14735.
    CleanExiHS_CDIPT.
    GenevestigatoriO14735.

    Organism-specific databases

    HPAiHPA045185.

    Interactioni

    Protein-protein interaction databases

    BioGridi115692. 12 interactions.
    IntActiO14735. 10 interactions.
    MINTiMINT-1150927.
    STRINGi9606.ENSP00000219789.

    Structurei

    3D structure databases

    ProteinModelPortaliO14735.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei6 – 2621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei29 – 4921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei76 – 9621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei140 – 16021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei175 – 19521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0558.
    HOGENOMiHOG000204051.
    HOVERGENiHBG050862.
    InParanoidiO14735.
    KOiK00999.
    OMAiIDVACHW.
    PhylomeDBiO14735.
    TreeFamiTF314603.

    Family and domain databases

    InterProiIPR000462. CDP-OH_P_trans.
    IPR014387. CDP_diag_ino_3_P_euk.
    [Graphical view]
    PfamiPF01066. CDP-OH_P_transf. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000848. CDP_diag_ino_3_P. 1 hit.
    PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O14735-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPDENIFLFV PNLIGYARIV FAIISFYFMP CCPLTASSFY LLSGLLDAFD    50
    GHAARALNQG TRFGAMLDML TDRCSTMCLL VNLALLYPGA TLFFQISMSL 100
    DVASHWLHLH SSVVRGSESH KMIDLSGNPV LRIYYTSRPA LFTLCAGNEL 150
    FYCLLYLFHF SEGPLVGSVG LFRMGLWVTA PIALLKSLIS VIHLITAARN 200
    MAALDAADRA KKK 213
    Length:213
    Mass (Da):23,539
    Last modified:January 1, 1998 - v1
    Checksum:i7885B72EF2A88093
    GO
    Isoform 2 (identifier: O14735-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-14: MPDENIFLFVPNLI → MLPTAAGFSI...PALSPRVPFQ
         111-129: SSVVRGSESHKMIDLSGNP → RSAAILGAWATWRHYSGVG
         130-213: Missing.

    Show »
    Length:182
    Mass (Da):19,455
    Checksum:i390F56D184377985
    GO
    Isoform 3 (identifier: O14735-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         15-59: Missing.

    Show »
    Length:168
    Mass (Da):18,624
    Checksum:i7941BB498981C00C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti75 – 751S → P in BAG62462. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti199 – 1991R → C.
    Corresponds to variant rs1802002 [ dbSNP | Ensembl ].
    VAR_048734

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 1414MPDEN…VPNLI → MLPTAAGFSIWGQVGAAREA PRCQTKISSCSCPTSSVSAA HGPGPNERARGLGGLPDPAL SPRVPFQ in isoform 2. 1 PublicationVSP_013618Add
    BLAST
    Alternative sequencei15 – 5945Missing in isoform 3. 1 PublicationVSP_054767Add
    BLAST
    Alternative sequencei111 – 12919SSVVR…LSGNP → RSAAILGAWATWRHYSGVG in isoform 2. 1 PublicationVSP_013619Add
    BLAST
    Alternative sequencei130 – 21384Missing in isoform 2. 1 PublicationVSP_013620Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF014807 mRNA. Translation: AAB94860.1.
    BT007301 mRNA. Translation: AAP35965.1.
    AK131349 mRNA. Translation: BAD18505.1.
    AK300805 mRNA. Translation: BAG62462.1.
    CR542016 mRNA. Translation: CAG46813.1.
    AC120114 Genomic DNA. No translation available.
    BC001444 mRNA. Translation: AAH01444.1.
    CCDSiCCDS10657.1. [O14735-1]
    CCDS67002.1. [O14735-3]
    RefSeqiNP_001273514.1. NM_001286585.1. [O14735-3]
    NP_006310.1. NM_006319.4. [O14735-1]
    UniGeneiHs.121549.

    Genome annotation databases

    EnsembliENST00000219789; ENSP00000219789; ENSG00000103502. [O14735-1]
    ENST00000566113; ENSP00000457340; ENSG00000103502. [O14735-3]
    ENST00000569956; ENSP00000457339; ENSG00000103502. [O14735-1]
    ENST00000570016; ENSP00000454453; ENSG00000103502. [O14735-1]
    GeneIDi10423.
    KEGGihsa:10423.
    UCSCiuc002dul.3. human. [O14735-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF014807 mRNA. Translation: AAB94860.1 .
    BT007301 mRNA. Translation: AAP35965.1 .
    AK131349 mRNA. Translation: BAD18505.1 .
    AK300805 mRNA. Translation: BAG62462.1 .
    CR542016 mRNA. Translation: CAG46813.1 .
    AC120114 Genomic DNA. No translation available.
    BC001444 mRNA. Translation: AAH01444.1 .
    CCDSi CCDS10657.1. [O14735-1 ]
    CCDS67002.1. [O14735-3 ]
    RefSeqi NP_001273514.1. NM_001286585.1. [O14735-3 ]
    NP_006310.1. NM_006319.4. [O14735-1 ]
    UniGenei Hs.121549.

    3D structure databases

    ProteinModelPortali O14735.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115692. 12 interactions.
    IntActi O14735. 10 interactions.
    MINTi MINT-1150927.
    STRINGi 9606.ENSP00000219789.

    PTM databases

    PhosphoSitei O14735.

    Proteomic databases

    MaxQBi O14735.
    PaxDbi O14735.
    PRIDEi O14735.

    Protocols and materials databases

    DNASUi 10423.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000219789 ; ENSP00000219789 ; ENSG00000103502 . [O14735-1 ]
    ENST00000566113 ; ENSP00000457340 ; ENSG00000103502 . [O14735-3 ]
    ENST00000569956 ; ENSP00000457339 ; ENSG00000103502 . [O14735-1 ]
    ENST00000570016 ; ENSP00000454453 ; ENSG00000103502 . [O14735-1 ]
    GeneIDi 10423.
    KEGGi hsa:10423.
    UCSCi uc002dul.3. human. [O14735-1 ]

    Organism-specific databases

    CTDi 10423.
    GeneCardsi GC16M029869.
    HGNCi HGNC:1769. CDIPT.
    HPAi HPA045185.
    MIMi 605893. gene.
    neXtProti NX_O14735.
    PharmGKBi PA26306.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0558.
    HOGENOMi HOG000204051.
    HOVERGENi HBG050862.
    InParanoidi O14735.
    KOi K00999.
    OMAi IDVACHW.
    PhylomeDBi O14735.
    TreeFami TF314603.

    Enzyme and pathway databases

    BioCyci MetaCyc:HS02513-MONOMER.
    Reactomei REACT_121000. Synthesis of PI.

    Miscellaneous databases

    GeneWikii CDIPT.
    GenomeRNAii 10423.
    NextBioi 35475278.
    PROi O14735.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O14735.
    Bgeei O14735.
    CleanExi HS_CDIPT.
    Genevestigatori O14735.

    Family and domain databases

    InterProi IPR000462. CDP-OH_P_trans.
    IPR014387. CDP_diag_ino_3_P_euk.
    [Graphical view ]
    Pfami PF01066. CDP-OH_P_transf. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000848. CDP_diag_ino_3_P. 1 hit.
    PROSITEi PS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The role of CDP-diacylglycerol synthetase and phosphatidylinositol synthase activity levels in the regulation of cellular phosphatidylinositol content."
      Lykidis A., Jackson P.D., Rock C.O., Jackowski S.
      J. Biol. Chem. 272:33402-33409(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Testis.
    2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
      Tissue: Thymus.
    4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    5. "The sequence and analysis of duplication-rich human chromosome 16."
      Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
      , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
      Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Placenta.
    7. "Purification and characterization of phosphatidylinositol synthase from human placenta."
      Antonsson B.E.
      Biochem. J. 297:517-522(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Placenta.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCDIPT_HUMAN
    AccessioniPrimary (citable) accession number: O14735
    Secondary accession number(s): B4DUV0
    , H3BTV1, Q6FGU1, Q6ZN70
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3