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O14732

- IMPA2_HUMAN

UniProt

O14732 - IMPA2_HUMAN

Protein

Inositol monophosphatase 2

Gene

IMPA2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Can use myo-inositol monophosphates, scylloinositol 1,4-diphosphate, glucose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates. Has been implicated as the pharmacological target for lithium Li+ action in brain.1 Publication

    Catalytic activityi

    Myo-inositol phosphate + H2O = myo-inositol + phosphate.

    Cofactori

    Magnesium.1 Publication

    Enzyme regulationi

    Inhibited by high Li+ and restricted Mg2+ concentrations.2 Publications

    Kineticsi

    1. KM=5 mM for myo-inositol 1-monophosphate1 Publication

    pH dependencei

    Optimum pH is 7.5-8.0.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi81 – 811Magnesium 1By similarity
    Binding sitei81 – 811Substrate1 Publication
    Metal bindingi101 – 1011Magnesium 1By similarity
    Metal bindingi101 – 1011Magnesium 2By similarity
    Metal bindingi103 – 1031Magnesium 1; via carbonyl oxygenBy similarity
    Metal bindingi104 – 1041Magnesium 2By similarity
    Binding sitei224 – 2241Substrate1 Publication
    Metal bindingi231 – 2311Magnesium 2By similarity
    Binding sitei231 – 2311SubstrateBy similarity

    GO - Molecular functioni

    1. inositol monophosphate 1-phosphatase activity Source: MGI
    2. inositol monophosphate 3-phosphatase activity Source: UniProtKB-EC
    3. inositol monophosphate 4-phosphatase activity Source: UniProtKB-EC
    4. metal ion binding Source: UniProtKB-KW
    5. protein homodimerization activity Source: MGI

    GO - Biological processi

    1. inositol biosynthetic process Source: UniProtKB-UniPathway
    2. inositol phosphate dephosphorylation Source: MGI
    3. inositol phosphate metabolic process Source: Reactome
    4. phosphate-containing compound metabolic process Source: ProtInc
    5. phosphatidylinositol phosphorylation Source: InterPro
    6. signal transduction Source: ProtInc
    7. small molecule metabolic process Source: Reactome

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:HS06822-MONOMER.
    BRENDAi3.1.3.25. 3474.
    ReactomeiREACT_150352. Synthesis of IP2, IP, and Ins in the cytosol.
    UniPathwayiUPA00823; UER00788.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inositol monophosphatase 2 (EC:3.1.3.25)
    Short name:
    IMP 2
    Short name:
    IMPase 2
    Alternative name(s):
    Inositol-1(or 4)-monophosphatase 2
    Myo-inositol monophosphatase A2
    Gene namesi
    Name:IMPA2
    Synonyms:IMP.18P
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 18

    Organism-specific databases

    HGNCiHGNC:6051. IMPA2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. cytosol Source: Reactome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi104 – 1041D → N: Loss of activity. 1 Publication

    Organism-specific databases

    PharmGKBiPA29861.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 288288Inositol monophosphatase 2PRO_0000142520Add
    BLAST

    Proteomic databases

    MaxQBiO14732.
    PaxDbiO14732.
    PRIDEiO14732.

    PTM databases

    PhosphoSiteiO14732.

    Expressioni

    Inductioni

    Repressed by Li+.

    Gene expression databases

    ArrayExpressiO14732.
    BgeeiO14732.
    CleanExiHS_IMPA2.
    GenevestigatoriO14732.

    Organism-specific databases

    HPAiHPA029561.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi109826. 2 interactions.
    IntActiO14732. 4 interactions.
    MINTiMINT-1413243.
    STRINGi9606.ENSP00000269159.

    Structurei

    Secondary structure

    1
    288
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi17 – 3822
    Helixi56 – 7015
    Beta strandi77 – 848
    Beta strandi97 – 1048
    Helixi106 – 1105
    Beta strandi117 – 1248
    Beta strandi127 – 1359
    Turni136 – 1394
    Beta strandi140 – 1456
    Turni146 – 1483
    Beta strandi150 – 1523
    Helixi165 – 1673
    Beta strandi169 – 1713
    Helixi180 – 19516
    Beta strandi199 – 2035
    Helixi207 – 2159
    Beta strandi220 – 2267
    Helixi229 – 2313
    Helixi233 – 2419
    Beta strandi245 – 2473
    Beta strandi251 – 2533
    Helixi256 – 2583
    Beta strandi260 – 2667
    Helixi267 – 2759

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CZHX-ray2.70A/B1-288[»]
    2CZIX-ray3.00A1-288[»]
    2CZKX-ray2.90A1-288[»]
    2DDKX-ray2.70A/B1-288[»]
    2FVZX-ray2.40A/B/C/D16-288[»]
    ProteinModelPortaliO14732.
    SMRiO14732. Positions 15-281.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO14732.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni103 – 1064Substrate binding
    Regioni205 – 2073Substrate binding

    Sequence similaritiesi

    Belongs to the inositol monophosphatase family.Curated

    Phylogenomic databases

    eggNOGiCOG0483.
    HOGENOMiHOG000282238.
    HOVERGENiHBG052123.
    InParanoidiO14732.
    KOiK01092.
    OMAiREMAMFI.
    OrthoDBiEOG7RJPS8.
    PhylomeDBiO14732.
    TreeFamiTF313194.

    Family and domain databases

    InterProiIPR020583. Inositol_monoP_metal-BS.
    IPR020552. Inositol_monoPase_Li-sen.
    IPR000760. Inositol_monophosphatase.
    IPR020550. Inositol_monophosphatase_CS.
    [Graphical view]
    PANTHERiPTHR20854. PTHR20854. 1 hit.
    PfamiPF00459. Inositol_P. 1 hit.
    [Graphical view]
    PRINTSiPR00377. IMPHPHTASES.
    PR00378. LIIMPHPHTASE.
    PROSITEiPS00629. IMP_1. 1 hit.
    PS00630. IMP_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O14732-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKPSGEDQAA LAAGPWEECF QAAVQLALRA GQIIRKALTE EKRVSTKTSA    50
    ADLVTETDHL VEDLIISELR ERFPSHRFIA EEAAASGAKC VLTHSPTWII 100
    DPIDGTCNFV HRFPTVAVSI GFAVRQELEF GVIYHCTEER LYTGRRGRGA 150
    FCNGQRLRVS GETDLSKALV LTEIGPKRDP ATLKLFLSNM ERLLHAKAHG 200
    VRVIGSSTLA LCHLASGAAD AYYQFGLHCW DLAAATVIIR EAGGIVIDTS 250
    GGPLDLMACR VVAASTREMA MLIAQALQTI NYGRDDEK 288
    Length:288
    Mass (Da):31,321
    Last modified:January 1, 1998 - v1
    Checksum:i30D83FA339AC69B4
    GO
    Isoform 2 (identifier: O14732-2) [UniParc]FASTAAdd to Basket

    Also known as: A2b

    The sequence of this isoform differs from the canonical sequence as follows:
         6-34: EDQAALAAGPWEECFQAAVQLALRAGQII → QD

    Show »
    Length:261
    Mass (Da):28,512
    Checksum:iA9D2BC0A05D0DF2F
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti88 – 881A → T.
    Corresponds to variant rs16976948 [ dbSNP | Ensembl ].
    VAR_049601

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei6 – 3429EDQAA…AGQII → QD in isoform 2. 1 PublicationVSP_013674Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF014398 mRNA. Translation: AAB70915.1.
    AF157102
    , AF157096, AF157097, AF157098, AF157099, AF157100, AF157101 Genomic DNA. Translation: AAD40683.1.
    AF200432 mRNA. Translation: AAF07824.1.
    BT007061 mRNA. Translation: AAP35710.1.
    EF444990 Genomic DNA. Translation: ACA06007.1.
    CH471113 Genomic DNA. Translation: EAX01559.1.
    BC017176 mRNA. Translation: AAH17176.1.
    CCDSiCCDS11855.1. [O14732-1]
    RefSeqiNP_055029.1. NM_014214.2. [O14732-1]
    UniGeneiHs.743311.

    Genome annotation databases

    EnsembliENST00000269159; ENSP00000269159; ENSG00000141401. [O14732-1]
    GeneIDi3613.
    KEGGihsa:3613.
    UCSCiuc002kqp.2. human. [O14732-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF014398 mRNA. Translation: AAB70915.1 .
    AF157102
    , AF157096 , AF157097 , AF157098 , AF157099 , AF157100 , AF157101 Genomic DNA. Translation: AAD40683.1 .
    AF200432 mRNA. Translation: AAF07824.1 .
    BT007061 mRNA. Translation: AAP35710.1 .
    EF444990 Genomic DNA. Translation: ACA06007.1 .
    CH471113 Genomic DNA. Translation: EAX01559.1 .
    BC017176 mRNA. Translation: AAH17176.1 .
    CCDSi CCDS11855.1. [O14732-1 ]
    RefSeqi NP_055029.1. NM_014214.2. [O14732-1 ]
    UniGenei Hs.743311.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CZH X-ray 2.70 A/B 1-288 [» ]
    2CZI X-ray 3.00 A 1-288 [» ]
    2CZK X-ray 2.90 A 1-288 [» ]
    2DDK X-ray 2.70 A/B 1-288 [» ]
    2FVZ X-ray 2.40 A/B/C/D 16-288 [» ]
    ProteinModelPortali O14732.
    SMRi O14732. Positions 15-281.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109826. 2 interactions.
    IntActi O14732. 4 interactions.
    MINTi MINT-1413243.
    STRINGi 9606.ENSP00000269159.

    Chemistry

    BindingDBi O14732.
    ChEMBLi CHEMBL1776.
    DrugBanki DB01356. Lithium.

    PTM databases

    PhosphoSitei O14732.

    Proteomic databases

    MaxQBi O14732.
    PaxDbi O14732.
    PRIDEi O14732.

    Protocols and materials databases

    DNASUi 3613.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000269159 ; ENSP00000269159 ; ENSG00000141401 . [O14732-1 ]
    GeneIDi 3613.
    KEGGi hsa:3613.
    UCSCi uc002kqp.2. human. [O14732-1 ]

    Organism-specific databases

    CTDi 3613.
    GeneCardsi GC18P011981.
    HGNCi HGNC:6051. IMPA2.
    HPAi HPA029561.
    MIMi 605922. gene.
    neXtProti NX_O14732.
    PharmGKBi PA29861.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0483.
    HOGENOMi HOG000282238.
    HOVERGENi HBG052123.
    InParanoidi O14732.
    KOi K01092.
    OMAi REMAMFI.
    OrthoDBi EOG7RJPS8.
    PhylomeDBi O14732.
    TreeFami TF313194.

    Enzyme and pathway databases

    UniPathwayi UPA00823 ; UER00788 .
    BioCyci MetaCyc:HS06822-MONOMER.
    BRENDAi 3.1.3.25. 3474.
    Reactomei REACT_150352. Synthesis of IP2, IP, and Ins in the cytosol.

    Miscellaneous databases

    ChiTaRSi IMPA2. human.
    EvolutionaryTracei O14732.
    GeneWikii IMPA2.
    GenomeRNAii 3613.
    NextBioi 14131.
    PROi O14732.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O14732.
    Bgeei O14732.
    CleanExi HS_IMPA2.
    Genevestigatori O14732.

    Family and domain databases

    InterProi IPR020583. Inositol_monoP_metal-BS.
    IPR020552. Inositol_monoPase_Li-sen.
    IPR000760. Inositol_monophosphatase.
    IPR020550. Inositol_monophosphatase_CS.
    [Graphical view ]
    PANTHERi PTHR20854. PTHR20854. 1 hit.
    Pfami PF00459. Inositol_P. 1 hit.
    [Graphical view ]
    PRINTSi PR00377. IMPHPHTASES.
    PR00378. LIIMPHPHTASE.
    PROSITEi PS00629. IMP_1. 1 hit.
    PS00630. IMP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel human myo-inositol monophosphatase gene, IMP.18p, maps to a susceptibility region for bipolar disorder."
      Yoshikawa T., Turner G., Esterling L.E., Sanders A.R., Detera-Wadleigh S.D.
      Mol. Psychiatry 2:393-397(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "A human myo-inositol monophosphatase gene (IMPA2) localized in a putative susceptibility region for bipolar disorder on chromosome 18p11.2: genomic structure and polymorphism screening in manic-depressive patients."
      Sjoeholt G., Gulbrandsen A.K., Lovlie R., Berle J., Molven A., Steen V.M.
      Mol. Psychiatry 5:172-180(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Molecular characterization of a novel form of human brain inositol monophosphatase A2b."
      Parthasarathy L., Parthasarathy R.
      Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    5. NHLBI resequencing and genotyping service (RS&G)
      Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Skin.
    8. "Lithium modulation of the human inositol monophosphatase 2 (IMPA2) promoter."
      Seelan R.S., Parthasarathy L.K., Parthasarathy R.N.
      Biochem. Biophys. Res. Commun. 324:1370-1378(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION.
    9. "Spatial expression patterns and biochemical properties distinguish a second myo-inositol monophosphatase IMPA2 from IMPA1."
      Ohnishi T., Ohba H., Seo K.-C., Im J., Sato Y., Iwayama Y., Furuichi T., Chung S.-K., Yoshikawa T.
      J. Biol. Chem. 282:637-646(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF ASP-104, ENZYME REGULATION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, HOMODIMERIZATION.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Crystal structure of human myo-inositol monophosphatase 2, the product of the putative susceptibility gene for bipolar disorder, schizophrenia, and febrile seizures."
      Arai R., Ito K., Ohnishi T., Ohba H., Akasaka R., Bessho Y., Hanawa-Suetsugu K., Yoshikawa T., Shirouzu M., Yokoyama S.
      Proteins 67:732-742(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) IN COMPLEX WITH SUBSTRATE, HOMODIMERIZATION.
    12. "Structure of human inositol monophosphatase 2."
      Structural genomics consortium (SGC)
      Submitted (FEB-2009) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 16-288.

    Entry informationi

    Entry nameiIMPA2_HUMAN
    AccessioniPrimary (citable) accession number: O14732
    Secondary accession number(s): B0YJ29, Q9UJT3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 18
      Human chromosome 18: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3