O14730 (RIOK3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase RIO3
EC=2.7.11.1

Alternative name(s):
RIO kinase 3
sudD homolog

Gene names

Name:	RIOK3
Synonyms:	SUDD

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Tissue specificity	Widely expressed. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chromosome segregation Traceable author statement Ref.1. Source: ProtInc
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CASP10	Q92851	4	EBI-1047061,EBI-495095

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O14730-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O14730-2)

The sequence of this isoform differs from the canonical sequence as follows:
     449-451: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 519	519	Serine/threonine-protein kinase RIO3		PRO_0000213529
Regions
	Domain	251 – 519	269	Protein kinase
	Nucleotide binding	257 – 265	9	ATP By similarity
Sites
	Active site	406	1	Proton acceptor By similarity
	Binding site	290	1	ATP By similarity
Amino acid modifications
	Modified residue	122	1	Phosphotyrosine Ref.4
	Modified residue	127	1	Phosphoserine Ref.4
	Modified residue	128	1	Phosphoserine Ref.4
Natural variations
	Alternative sequence	449 – 451	3	Missing in isoform 2.		VSP_012164
	Natural variant	336	1	L → V. Ref.6 Corresponds to variant rs35401850 [ dbSNP | Ensembl ].		VAR_042355
	Natural variant	441	1	R → Q. Ref.6 Corresponds to variant rs33969048 [ dbSNP | Ensembl ].		VAR_042356
	Natural variant	447	1	S → L. Ref.6 Corresponds to variant rs56282762 [ dbSNP | Ensembl ].		VAR_042357
Experimental info
	Sequence conflict	325	1	I → H in AAC26080. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 20, 2007. Version 2. Checksum: 3C3392BAC4ECB912	FASTA	519	59,093
	10 20 30 40 50 60 MDLVGVASPE PGTAAAWGPS KCPWAIPQNT ISCSLADVMS EQLAKELQLE EEAAVFPEVA 70 80 90 100 110 120 VAEGPFITGE NIDTSSDLML AQMLQMEYDR EYDAQLRREE KKFNGDSKVS ISFENYRKVH 130 140 150 160 170 180 PYEDSDSSED EVDWQDTRDD PYRPAKPVPT PKKGFIGKGK DITTKHDEVV CGRKNTARME 190 200 210 220 230 240 NFAPEFQVGD GIGMDLKLSN HVFNALKQHA YSEERRSARL HEKKEHSTAE KAVDPKTRLL 250 260 270 280 290 300 MYKMVNSGML ETITGCISTG KESVVFHAYG GSMEDEKEDS KVIPTECAIK VFKTTLNEFK 310 320 330 340 350 360 NRDKYIKDDF RFKDRFSKLN PRKIIRMWAE KEMHNLARMQ RAGIPCPTVV LLKKHILVMS 370 380 390 400 410 420 FIGHDQVPAP KLKEVKLNSE EMKEAYYQTL HLMRQLYHEC TLVHADLSEY NMLWHAGKVW 430 440 450 460 470 480 LIDVSQSVEP THPHGLEFLF RDCRNVSQFF QKGGVKEALS ERELFNAVSG LNITADNEAD 490 500 510 FLAEIEALEK MNEDHVQKNG RKAASFLKDD GDPPLLYDE
	Isoform 2 [UniParc]. Checksum: 2CA9BC88DD00CFE5	FASTA	516	58,671
	10 20 30 40 50 60 MDLVGVASPE PGTAAAWGPS KCPWAIPQNT ISCSLADVMS EQLAKELQLE EEAAVFPEVA 70 80 90 100 110 120 VAEGPFITGE NIDTSSDLML AQMLQMEYDR EYDAQLRREE KKFNGDSKVS ISFENYRKVH 130 140 150 160 170 180 PYEDSDSSED EVDWQDTRDD PYRPAKPVPT PKKGFIGKGK DITTKHDEVV CGRKNTARME 190 200 210 220 230 240 NFAPEFQVGD GIGMDLKLSN HVFNALKQHA YSEERRSARL HEKKEHSTAE KAVDPKTRLL 250 260 270 280 290 300 MYKMVNSGML ETITGCISTG KESVVFHAYG GSMEDEKEDS KVIPTECAIK VFKTTLNEFK 310 320 330 340 350 360 NRDKYIKDDF RFKDRFSKLN PRKIIRMWAE KEMHNLARMQ RAGIPCPTVV LLKKHILVMS 370 380 390 400 410 420 FIGHDQVPAP KLKEVKLNSE EMKEAYYQTL HLMRQLYHEC TLVHADLSEY NMLWHAGKVW 430 440 450 460 470 480 LIDVSQSVEP THPHGLEFLF RDCRNVSQKG GVKEALSERE LFNAVSGLNI TADNEADFLA 490 500 510 EIEALEKMNE DHVQKNGRKA ASFLKDDGDP PLLYDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of the Aspergillus nidulans sudD gene and its human homologue." Anaya P., Evans S.C., Dai C., Lozano G., May G.S. Gene 211:323-329(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]	"DNA sequence and analysis of human chromosome 18." Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J. , Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L., Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A., Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C., Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K., Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R., Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S. Nature 437:551-555(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[4]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-122; SER-127 AND SER-128, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] VAL-336; GLN-441 AND LEU-447.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF013591 mRNA. Translation: AAC26080.1. AC026634 Genomic DNA. No translation available. BC039729 mRNA. Translation: AAH39729.1.
IPI	IPI00298199. IPI00375164.
RefSeq	NP_003822.2. NM_003831.3.
UniGene	Hs.445511.
3D structure databases
ProteinModelPortal	O14730.
ModBase	Search...
Protein-protein interaction databases
IntAct	O14730. 6 interactions.
STRING	9606.ENSP00000341874.
PTM databases
PhosphoSite	O14730.
Proteomic databases
PaxDb	O14730.
PRIDE	O14730.
Protocols and materials databases
DNASU	8780.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000339486; ENSP00000341874; ENSG00000101782. ENST00000577501; ENSP00000462548; ENSG00000101782.
GeneID	8780.
KEGG	hsa:8780.
UCSC	uc002kui.4. human. uc010dls.3. human.
Organism-specific databases
CTD	8780.
GeneCards	GC18P021032.
HGNC	HGNC:11451. RIOK3.
HPA	HPA001620. HPA024184.
MIM	603579. gene.
neXtProt	NX_O14730.
PharmGKB	PA36248.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1718.
HOGENOM	HOG000007623.
HOVERGEN	HBG057475.
InParanoid	O14730.
KO	K08872.
OMA	KMNEDHV.
OrthoDB	EOG4FFD1M.
PhylomeDB	O14730.
Gene expression databases
ArrayExpress	O14730.
Bgee	O14730.
CleanEx	HS_RIOK3.
Genevestigator	O14730.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR018934. RIO-like_kinase. IPR000687. RIO_kinase. IPR018935. RIO_kinase_CS. IPR017406. Ser/Thr_kinase_Rio3. [Graphical view]
Pfam	PF01163. RIO1. 1 hit. [Graphical view]
PIRSF	PIRSF038146. Ser/Thr_PK_RIO3. 1 hit.
SMART	SM00090. RIO. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS50011. PROTEIN_KINASE_DOM. False negative. PS01245. RIO1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	O14730.
ChEMBL	CHEMBL5659.
GenomeRNAi	8780.
NextBio	32920.
SOURCE	Search...

Entry information

Entry name

RIOK3_HUMAN

Accession

Primary (citable) accession number: O14730
Secondary accession number(s): Q8IXN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	February 20, 2007
Last modified:	May 1, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.