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O14727

- APAF_HUMAN

UniProt

O14727 - APAF_HUMAN

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Protein

Apoptotic protease-activating factor 1

Gene
APAF1, KIAA0413
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei265 – 2651ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi154 – 1618ATP Reviewed prediction

GO - Molecular functioni

  1. ADP binding Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. cysteine-type endopeptidase activator activity involved in apoptotic process Source: UniProtKB
  4. nucleotide binding Source: UniProtKB
  5. protein binding Source: UniProtKB

GO - Biological processi

  1. activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  2. activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c Source: UniProtKB
  3. apoptotic DNA fragmentation Source: Ensembl
  4. apoptotic process Source: Reactome
  5. defense response Source: InterPro
  6. forebrain development Source: Ensembl
  7. intrinsic apoptotic signaling pathway Source: UniProtKB
  8. nervous system development Source: ProtInc
  9. neural tube closure Source: Ensembl
  10. neuron apoptotic process Source: Ensembl
  11. positive regulation of apoptotic process Source: UniProtKB
  12. positive regulation of apoptotic signaling pathway Source: Ensembl
  13. regulation of apoptotic process Source: UniProtKB
  14. response to G1 DNA damage checkpoint signaling Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

ATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_607. Activation of caspases through apoptosome-mediated cleavage.
REACT_89. Formation of apoptosome.
SABIO-RKO14727.
SignaLinkiO14727.

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptotic protease-activating factor 1
Short name:
APAF-1
Gene namesi
Name:APAF1
Synonyms:KIAA0413
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 12

Organism-specific databases

HGNCiHGNC:576. APAF1.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. apoptosome Source: UniProtKB
  2. cytosol Source: Reactome
  3. extracellular vesicular exosome Source: UniProt
  4. nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi160 – 1601K → R: No association with APAF-1. No binding to pro-caspase-9. 1 Publication
Mutagenesisi368 – 3681M → L: Activation of pro-caspase-9 independent of cytochrome c. Increased ability to induce apoptosis. 1 Publication

Organism-specific databases

PharmGKBiPA24868.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12481248Apoptotic protease-activating factor 1PRO_0000050844Add
BLAST

Proteomic databases

MaxQBiO14727.
PaxDbiO14727.
PRIDEiO14727.

PTM databases

PhosphoSiteiO14727.

Miscellaneous databases

PMAP-CutDBO14727.

Expressioni

Tissue specificityi

Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.

Inductioni

By E2F and p53/TP53 in apoptotic neurons.1 Publication

Gene expression databases

ArrayExpressiO14727.
BgeeiO14727.
GenevestigatoriO14727.

Organism-specific databases

HPAiHPA031373.

Interactioni

Subunit structurei

Monomer. Oligomerizes to a heptameric ring, known as the apoptosome, upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CASP9P5521118EBI-446492,EBI-516799
CYCSP999996EBI-446492,EBI-446479
PPP1CAP621362EBI-446492,EBI-357253
YWHAEP622582EBI-446492,EBI-356498

Protein-protein interaction databases

BioGridi106814. 16 interactions.
DIPiDIP-27624N.
IntActiO14727. 7 interactions.
MINTiMINT-96751.

Structurei

Secondary structure

1
1248
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 119
Helixi13 – 197
Helixi22 – 3211
Helixi37 – 448
Beta strandi46 – 483
Helixi49 – 6113
Helixi65 – 7713
Helixi81 – 877
Helixi88 – 903
Helixi108 – 1169
Helixi130 – 14011
Beta strandi148 – 1536
Helixi160 – 1689
Helixi171 – 1777
Beta strandi182 – 1898
Helixi192 – 20615
Helixi220 – 23314
Beta strandi239 – 2457
Helixi248 – 2525
Beta strandi259 – 2657
Helixi267 – 2704
Beta strandi277 – 2815
Helixi288 – 29912
Helixi303 – 3053
Helixi309 – 3168
Turni317 – 3193
Helixi321 – 33313
Helixi338 – 3469
Helixi362 – 37312
Turni377 – 3793
Helixi380 – 3856
Helixi386 – 3883
Helixi397 – 4048
Helixi408 – 42013
Beta strandi423 – 4297
Beta strandi432 – 4365
Helixi439 – 44810
Helixi450 – 4523
Helixi453 – 46412
Turni465 – 4673
Helixi470 – 4723
Helixi480 – 49314
Helixi497 – 5048
Helixi507 – 51711
Helixi520 – 5289
Helixi530 – 5323
Helixi535 – 55016
Turni551 – 5566
Helixi563 – 5675
Helixi575 – 58511

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1C15NMR-A1-97[»]
1CWWNMR-A1-97[»]
1CY5X-ray1.30A1-97[»]
1Z6TX-ray2.21A/B/C/D1-591[»]
2P1HX-ray1.59A1-92[»]
2YGSX-ray1.60A1-92[»]
3J2Telectron microscopy9.5A/B/C/D/E/F/G1-1248[»]
3YGSX-ray2.50C1-95[»]
ProteinModelPortaliO14727.
SMRiO14727. Positions 1-1248.

Miscellaneous databases

EvolutionaryTraceiO14727.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9090CARDAdd
BLAST
Domaini104 – 415312NB-ARCAdd
BLAST
Repeati613 – 65240WD 1-1Add
BLAST
Repeati655 – 69440WD 1-2Add
BLAST
Repeati697 – 73842WD 1-3Add
BLAST
Repeati741 – 78040WD 1-4Add
BLAST
Repeati796 – 83641WD 1-5Add
BLAST
Repeati838 – 87740WD 1-6Add
BLAST
Repeati880 – 91031WD 1-7Add
BLAST
Repeati922 – 95837WD 2-1Add
BLAST
Repeati959 – 99840WD 2-2Add
BLAST
Repeati1001 – 104040WD 2-3Add
BLAST
Repeati1042 – 108039WD 2-4Add
BLAST
Repeati1083 – 112240WD 2-5Add
BLAST
Repeati1125 – 116440WD 2-6Add
BLAST
Repeati1175 – 121238WD 2-7Add
BLAST
Repeati1213 – 124836WD 2-8Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni910 – 92112Interpropeller linker By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi95 – 984Poly-Ser

Domaini

The CARD domain mediates interaction with APIP.
The monomeric form is autoinhibited in a closed conformation through a bound ADP at the nucleotide binding site. Exchange of ADP for ATP and binding of cytochrome c trigger a large conformational change where the first WD repeat region swings out, allowing the NB-ARC domain to rotate and expose the contact areas for oligomerization By similarity.

Sequence similaritiesi

Contains 1 CARD domain.
Contains 1 NB-ARC domain.
Contains 15 WD repeats.

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
HOVERGENiHBG018730.
InParanoidiO14727.
KOiK02084.
OMAiETKKVCK.
OrthoDBiEOG7XWPMS.
PhylomeDBiO14727.
TreeFamiTF323866.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
2.130.10.10. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR017251. Apoptotic_pept-activating_1.
IPR001315. CARD.
IPR011029. DEATH-like_dom.
IPR000767. Disease_R.
IPR020472. G-protein_beta_WD-40_rep.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00619. CARD. 1 hit.
PF00931. NB-ARC. 1 hit.
PF00400. WD40. 10 hits.
[Graphical view]
PIRSFiPIRSF037646. Apop_pept_activating-1. 1 hit.
PRINTSiPR00364. DISEASERSIST.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 13 hits.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
SSF50978. SSF50978. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50209. CARD. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 9 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O14727-1) [UniParc]FASTAAdd to Basket

Also known as: Apaf-1XL

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDAKARNCLL QHREALEKDI KTSYIMDHMI SDGFLTISEE EKVRNEPTQQ     50
QRAAMLIKMI LKKDNDSYVS FYNALLHEGY KDLAALLHDG IPVVSSSSGK 100
DSVSGITSYV RTVLCEGGVP QRPVVFVTRK KLVNAIQQKL SKLKGEPGWV 150
TIHGMAGCGK SVLAAEAVRD HSLLEGCFPG GVHWVSVGKQ DKSGLLMKLQ 200
NLCTRLDQDE SFSQRLPLNI EEAKDRLRIL MLRKHPRSLL ILDDVWDSWV 250
LKAFDSQCQI LLTTRDKSVT DSVMGPKYVV PVESSLGKEK GLEILSLFVN 300
MKKADLPEQA HSIIKECKGS PLVVSLIGAL LRDFPNRWEY YLKQLQNKQF 350
KRIRKSSSYD YEALDEAMSI SVEMLREDIK DYYTDLSILQ KDVKVPTKVL 400
CILWDMETEE VEDILQEFVN KSLLFCDRNG KSFRYYLHDL QVDFLTEKNC 450
SQLQDLHKKI ITQFQRYHQP HTLSPDQEDC MYWYNFLAYH MASAKMHKEL 500
CALMFSLDWI KAKTELVGPA HLIHEFVEYR HILDEKDCAV SENFQEFLSL 550
NGHLLGRQPF PNIVQLGLCE PETSEVYQQA KLQAKQEVDN GMLYLEWINK 600
KNITNLSRLV VRPHTDAVYH ACFSEDGQRI ASCGADKTLQ VFKAETGEKL 650
LEIKAHEDEV LCCAFSTDDR FIATCSVDKK VKIWNSMTGE LVHTYDEHSE 700
QVNCCHFTNS SHHLLLATGS SDCFLKLWDL NQKECRNTMF GHTNSVNHCR 750
FSPDDKLLAS CSADGTLKLW DATSANERKS INVKQFFLNL EDPQEDMEVI 800
VKCCSWSADG ARIMVAAKNK IFLFDIHTSG LLGEIHTGHH STIQYCDFSP 850
QNHLAVVALS QYCVELWNTD SRSKVADCRG HLSWVHGVMF SPDGSSFLTS 900
SDDQTIRLWE TKKVCKNSAV MLKQEVDVVF QENEVMVLAV DHIRRLQLIN 950
GRTGQIDYLT EAQVSCCCLS PHLQYIAFGD ENGAIEILEL VNNRIFQSRF 1000
QHKKTVWHIQ FTADEKTLIS SSDDAEIQVW NWQLDKCIFL RGHQETVKDF 1050
RLLKNSRLLS WSFDGTVKVW NIITGNKEKD FVCHQGTVLS CDISHDATKF 1100
SSTSADKTAK IWSFDLLLPL HELRGHNGCV RCSAFSVDST LLATGDDNGE 1150
IRIWNVSNGE LLHLCAPLSE EGAATHGGWV TDLCFSPDGK MLISAGGYIK 1200
WWNVVTGESS QTFYTNGTNL KKIHVSPDFK TYVTVDNLGI LYILQTLE 1248
Length:1,248
Mass (Da):141,840
Last modified:January 23, 2002 - v2
Checksum:i0750D05817AC9B3B
GO
Isoform 2 (identifier: O14727-2) [UniParc]FASTAAdd to Basket

Also known as: Apaf-1L

The sequence of this isoform differs from the canonical sequence as follows:
     99-109: Missing.

Show »
Length:1,237
Mass (Da):140,745
Checksum:i0D3EF9AB2A66FFF5
GO
Isoform 3 (identifier: O14727-3) [UniParc]FASTAAdd to Basket

Also known as: Apaf-1S

The sequence of this isoform differs from the canonical sequence as follows:
     99-109: Missing.
     824-866: Missing.

Show »
Length:1,194
Mass (Da):135,980
Checksum:iA675EA102DDAAFB7
GO
Isoform 4 (identifier: O14727-4) [UniParc]FASTAAdd to Basket

Also known as: Apaf-1M

The sequence of this isoform differs from the canonical sequence as follows:
     824-866: Missing.

Show »
Length:1,205
Mass (Da):137,076
Checksum:iDA57B4E35E79D73A
GO
Isoform 5 (identifier: O14727-5) [UniParc]FASTAAdd to Basket

Also known as: Apaf-1XS

The sequence of this isoform differs from the canonical sequence as follows:
     575-575: E → ETLGFESKK
     824-866: Missing.
     1113-1154: Missing.

Show »
Length:1,171
Mass (Da):133,356
Checksum:iCA9B14019EAB35BF
GO
Isoform 6 (identifier: O14727-6) [UniParc]FASTAAdd to Basket

Also known as: Apaf-1-ALT

The sequence of this isoform differs from the canonical sequence as follows:
     319-338: GSPLVVSLIGALLRDFPNRW → VVERCHWGILTDLLHKWNQS
     339-1248: Missing.

Show »
Length:338
Mass (Da):37,976
Checksum:i29D933A65707ED92
GO

Sequence cautioni

The sequence AAK28401.1 differs from that shown. Reason: Frameshift at position 108.
The sequence BAA24843.2 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei99 – 10911Missing in isoform 2 and isoform 3. VSP_006759Add
BLAST
Alternative sequencei319 – 33820GSPLV…FPNRW → VVERCHWGILTDLLHKWNQS in isoform 6. VSP_008965Add
BLAST
Alternative sequencei339 – 1248910Missing in isoform 6. VSP_008966Add
BLAST
Alternative sequencei575 – 5751E → ETLGFESKK in isoform 5. VSP_006760
Alternative sequencei824 – 86643Missing in isoform 3, isoform 4 and isoform 5. VSP_006761Add
BLAST
Alternative sequencei1113 – 115442Missing in isoform 5. VSP_006762Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti134 – 1341N → S1 Publication
Sequence conflicti145 – 1451G → C in CAB55587. 1 Publication
Sequence conflicti161 – 1611S → F in CAB55586. 1 Publication
Sequence conflicti370 – 3701I → T in CAB55581. 1 Publication
Sequence conflicti383 – 3831Y → H in CAB55586. 1 Publication
Sequence conflicti544 – 5441F → L in CAB55584. 1 Publication
Sequence conflicti580 – 5801A → T in CAB55580. 1 Publication
Sequence conflicti608 – 6081R → C in CAB55585. 1 Publication
Sequence conflicti620 – 6201H → R in CAB55587. 1 Publication
Sequence conflicti639 – 6391L → F in CAB55583. 1 Publication
Sequence conflicti708 – 7081T → A in CAB55579. 1 Publication
Sequence conflicti742 – 7421H → R in CAB55584. 1 Publication
Sequence conflicti746 – 7461V → A in CAB55586. 1 Publication
Sequence conflicti757 – 7571L → P in CAB56462. 1 Publication
Sequence conflicti795 – 7951E → G in CAB55581. 1 Publication
Sequence conflicti798 – 7981E → G in CAB55587. 1 Publication
Sequence conflicti825 – 8251D → A in CAB55585. 1 Publication
Sequence conflicti871 – 8711S → L in CAB55587. 1 Publication
Sequence conflicti876 – 8761A → T in CAB55581. 1 Publication
Sequence conflicti949 – 9491I → V in CAB55585. 1 Publication
Sequence conflicti1008 – 10081H → R in CAB55582. 1 Publication
Sequence conflicti1056 – 10561S → P in CAB55582. 1 Publication
Sequence conflicti1241 – 12411L → I in BAA24843. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF013263 mRNA. Translation: AAC51678.1.
AJ243003 mRNA. Translation: CAB55579.1.
AJ243004 mRNA. Translation: CAB55580.1.
AJ243005 mRNA. Translation: CAB55581.1.
AJ243006 mRNA. Translation: CAB55582.1.
AJ243007 mRNA. Translation: CAB55583.1.
AJ243008 mRNA. Translation: CAB55584.1.
AJ243009 mRNA. Translation: CAB55585.1.
AJ243010 mRNA. Translation: CAB55586.1.
AJ243011 mRNA. Translation: CAB55587.1.
AJ243048 mRNA. Translation: CAB55588.1.
AJ243107 mRNA. Translation: CAB56462.1.
AF134397 mRNA. Translation: AAD38344.1.
AF149794 mRNA. Translation: AAD34016.1.
AB007873 mRNA. Translation: BAA24843.2. Different initiation.
AB103079 mRNA. Translation: BAC77343.1.
CH471054 Genomic DNA. Translation: EAW97606.1.
BC136531 mRNA. Translation: AAI36532.1.
BC136532 mRNA. Translation: AAI36533.1.
AJ133643 Genomic DNA. Translation: CAB65085.1.
AJ133644 Genomic DNA. Translation: CAB65086.1.
AJ133645 Genomic DNA. Translation: CAB65087.1.
AF248734 mRNA. Translation: AAK28401.1. Frameshift.
CCDSiCCDS55862.1. [O14727-2]
CCDS55863.1. [O14727-3]
CCDS9069.1. [O14727-1]
CCDS9070.1. [O14727-4]
CCDS9071.1. [O14727-6]
PIRiT03818.
RefSeqiNP_001151.1. NM_001160.2. [O14727-3]
NP_037361.1. NM_013229.2. [O14727-2]
NP_863651.1. NM_181861.1. [O14727-1]
NP_863658.1. NM_181868.1. [O14727-4]
NP_863659.1. NM_181869.1. [O14727-6]
UniGeneiHs.552567.

Genome annotation databases

EnsembliENST00000333991; ENSP00000334558; ENSG00000120868. [O14727-6]
ENST00000357310; ENSP00000349862; ENSG00000120868. [O14727-4]
ENST00000359972; ENSP00000353059; ENSG00000120868. [O14727-3]
ENST00000547045; ENSP00000449791; ENSG00000120868. [O14727-4]
ENST00000550527; ENSP00000448449; ENSG00000120868. [O14727-2]
ENST00000551964; ENSP00000448165; ENSG00000120868. [O14727-1]
ENST00000552268; ENSP00000448826; ENSG00000120868. [O14727-6]
GeneIDi317.
KEGGihsa:317.
UCSCiuc001tfy.3. human. [O14727-2]
uc001tfz.3. human. [O14727-1]
uc001tga.3. human. [O14727-3]
uc001tgb.3. human. [O14727-4]
uc001tgc.3. human. [O14727-6]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF013263 mRNA. Translation: AAC51678.1 .
AJ243003 mRNA. Translation: CAB55579.1 .
AJ243004 mRNA. Translation: CAB55580.1 .
AJ243005 mRNA. Translation: CAB55581.1 .
AJ243006 mRNA. Translation: CAB55582.1 .
AJ243007 mRNA. Translation: CAB55583.1 .
AJ243008 mRNA. Translation: CAB55584.1 .
AJ243009 mRNA. Translation: CAB55585.1 .
AJ243010 mRNA. Translation: CAB55586.1 .
AJ243011 mRNA. Translation: CAB55587.1 .
AJ243048 mRNA. Translation: CAB55588.1 .
AJ243107 mRNA. Translation: CAB56462.1 .
AF134397 mRNA. Translation: AAD38344.1 .
AF149794 mRNA. Translation: AAD34016.1 .
AB007873 mRNA. Translation: BAA24843.2 . Different initiation.
AB103079 mRNA. Translation: BAC77343.1 .
CH471054 Genomic DNA. Translation: EAW97606.1 .
BC136531 mRNA. Translation: AAI36532.1 .
BC136532 mRNA. Translation: AAI36533.1 .
AJ133643 Genomic DNA. Translation: CAB65085.1 .
AJ133644 Genomic DNA. Translation: CAB65086.1 .
AJ133645 Genomic DNA. Translation: CAB65087.1 .
AF248734 mRNA. Translation: AAK28401.1 . Frameshift.
CCDSi CCDS55862.1. [O14727-2 ]
CCDS55863.1. [O14727-3 ]
CCDS9069.1. [O14727-1 ]
CCDS9070.1. [O14727-4 ]
CCDS9071.1. [O14727-6 ]
PIRi T03818.
RefSeqi NP_001151.1. NM_001160.2. [O14727-3 ]
NP_037361.1. NM_013229.2. [O14727-2 ]
NP_863651.1. NM_181861.1. [O14727-1 ]
NP_863658.1. NM_181868.1. [O14727-4 ]
NP_863659.1. NM_181869.1. [O14727-6 ]
UniGenei Hs.552567.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1C15 NMR - A 1-97 [» ]
1CWW NMR - A 1-97 [» ]
1CY5 X-ray 1.30 A 1-97 [» ]
1Z6T X-ray 2.21 A/B/C/D 1-591 [» ]
2P1H X-ray 1.59 A 1-92 [» ]
2YGS X-ray 1.60 A 1-92 [» ]
3J2T electron microscopy 9.5 A/B/C/D/E/F/G 1-1248 [» ]
3YGS X-ray 2.50 C 1-95 [» ]
ProteinModelPortali O14727.
SMRi O14727. Positions 1-1248.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 106814. 16 interactions.
DIPi DIP-27624N.
IntActi O14727. 7 interactions.
MINTi MINT-96751.

Chemistry

BindingDBi O14727.
ChEMBLi CHEMBL1795093.
DrugBanki DB00171. Adenosine triphosphate.

PTM databases

PhosphoSitei O14727.

Proteomic databases

MaxQBi O14727.
PaxDbi O14727.
PRIDEi O14727.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000333991 ; ENSP00000334558 ; ENSG00000120868 . [O14727-6 ]
ENST00000357310 ; ENSP00000349862 ; ENSG00000120868 . [O14727-4 ]
ENST00000359972 ; ENSP00000353059 ; ENSG00000120868 . [O14727-3 ]
ENST00000547045 ; ENSP00000449791 ; ENSG00000120868 . [O14727-4 ]
ENST00000550527 ; ENSP00000448449 ; ENSG00000120868 . [O14727-2 ]
ENST00000551964 ; ENSP00000448165 ; ENSG00000120868 . [O14727-1 ]
ENST00000552268 ; ENSP00000448826 ; ENSG00000120868 . [O14727-6 ]
GeneIDi 317.
KEGGi hsa:317.
UCSCi uc001tfy.3. human. [O14727-2 ]
uc001tfz.3. human. [O14727-1 ]
uc001tga.3. human. [O14727-3 ]
uc001tgb.3. human. [O14727-4 ]
uc001tgc.3. human. [O14727-6 ]

Organism-specific databases

CTDi 317.
GeneCardsi GC12P099039.
HGNCi HGNC:576. APAF1.
HPAi HPA031373.
MIMi 602233. gene.
neXtProti NX_O14727.
PharmGKBi PA24868.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG2319.
HOVERGENi HBG018730.
InParanoidi O14727.
KOi K02084.
OMAi ETKKVCK.
OrthoDBi EOG7XWPMS.
PhylomeDBi O14727.
TreeFami TF323866.

Enzyme and pathway databases

Reactomei REACT_607. Activation of caspases through apoptosome-mediated cleavage.
REACT_89. Formation of apoptosome.
SABIO-RK O14727.
SignaLinki O14727.

Miscellaneous databases

EvolutionaryTracei O14727.
GeneWikii APAF1.
GenomeRNAii 317.
NextBioi 1287.
PMAP-CutDB O14727.
PROi O14727.
SOURCEi Search...

Gene expression databases

ArrayExpressi O14727.
Bgeei O14727.
Genevestigatori O14727.

Family and domain databases

Gene3Di 1.10.533.10. 1 hit.
2.130.10.10. 2 hits.
3.40.50.300. 1 hit.
InterProi IPR017251. Apoptotic_pept-activating_1.
IPR001315. CARD.
IPR011029. DEATH-like_dom.
IPR000767. Disease_R.
IPR020472. G-protein_beta_WD-40_rep.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF00619. CARD. 1 hit.
PF00931. NB-ARC. 1 hit.
PF00400. WD40. 10 hits.
[Graphical view ]
PIRSFi PIRSF037646. Apop_pept_activating-1. 1 hit.
PRINTSi PR00364. DISEASERSIST.
PR00320. GPROTEINBRPT.
SMARTi SM00320. WD40. 13 hits.
[Graphical view ]
SUPFAMi SSF47986. SSF47986. 1 hit.
SSF50978. SSF50978. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEi PS50209. CARD. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 9 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3."
    Zou H., Henzel W.J., Liu X., Lutschg A., Wang X.
    Cell 90:405-413(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), PARTIAL PROTEIN SEQUENCE.
    Tissue: Cervix carcinoma.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 4 AND 5).
    Tissue: Cervix carcinoma, Heart and Peripheral blood.
  3. "Cytochrome c and dATP-mediated oligomerization of Apaf-1 is a prerequisite for procaspase-9 activation."
    Saleh A., Srinivasula S.M., Acharya S., Fishel R., Alnemri E.S.
    J. Biol. Chem. 274:17941-17945(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: T-cell.
  4. "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis."
    Hu Y., Benedict M.A., Ding L., Nunez G.
    EMBO J. 18:3586-3595(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBUNIT, MUTAGENESIS OF LYS-160 AND MET-368.
    Tissue: Kidney.
  5. "APAF-1-ALT, a novel alternative splicing form of APAF-1, potentially causes impeded ability of undergoing DNA damage-induced apoptosis in the LNCaP human prostate cancer cell line."
    Ogawa T., Shiga K., Hashimoto S., Kobayashi T., Horii A., Furukawa T.
    Biochem. Biophys. Res. Commun. 306:537-543(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 6), FUNCTION, SUBCELLULAR LOCATION.
    Tissue: Prostatic carcinoma.
  6. "Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro."
    Ishikawa K., Nagase T., Nakajima D., Seki N., Ohira M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 4:307-313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  7. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
    Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
    DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  10. "The mammalian CED4 homologue, APAF1, exists as two distinct forms in human cells."
    Roberts D.L., Dalgleish R., Cohen G.M., MacFarlane M.
    Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 810-864 AND 866-883.
  11. "Cloning of variant Apaf1."
    Won M., Lee J.-W., Ohr H.-H., Kim D.-U., Chung K.-S., Lee M., Yoo H.-S.
    Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-138 (ISOFORMS 1/4/5).
  12. "Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization."
    Srinivasula S.M., Ahmad M., Fernandes-Alnemri T., Alnemri E.S.
    Mol. Cell 1:949-957(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: APAF-1-MEDIATED OLIGOMERIZATION.
  13. Cited for: INDUCTION BY E2F AND TP53.
  14. "Induced inhibition of ischemic/hypoxic injury by APIP, a novel Apaf-1-interacting protein."
    Cho D.-H., Hong Y.-M., Lee H.-J., Woo H.-N., Pyo J.-O., Mak T.W., Jung Y.-K.
    J. Biol. Chem. 279:39942-39950(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH APIP.
  15. "Calcium blocks formation of apoptosome by preventing nucleotide exchange in Apaf-1."
    Bao Q., Lu W., Rabinowitz J.D., Shi Y.
    Mol. Cell 25:181-192(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INHIBITION BY CALCIUM.
  16. "Integrity of ATP binding site is essential for effective inhibition of the intrinsic apoptosis pathway by NAIP."
    Karimpour S., Davoodi J., Ghahremani M.H.
    Biochem. Biophys. Res. Commun. 407:158-162(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NAIP/BIRC1.
  17. "Crystal structure of Apaf-1 caspase recruitment domain: an alpha-helical Greek key fold for apoptotic signaling."
    Vaughn D.E., Rodriguez J., Lazebnik Y., Joshua-Tor L.
    J. Mol. Biol. 293:439-447(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS) OF 1-97.
  18. "Solution structure and mutagenesis of the caspase recruitment domain (CARD) from Apaf-1."
    Day C.L., Dupont C., Lackmann M., Vaux D.L., Hinds M.G.
    Cell Death Differ. 6:1125-1132(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 1-97.

Entry informationi

Entry nameiAPAF_HUMAN
AccessioniPrimary (citable) accession number: O14727
Secondary accession number(s): B2RMX8
, O43297, Q7Z438, Q9BXZ6, Q9UBZ5, Q9UGN8, Q9UGN9, Q9UGP0, Q9UJ58, Q9UJ59, Q9UJ60, Q9UJ61, Q9UJ62, Q9UJ63, Q9UJ64, Q9UJ65, Q9UJ66, Q9UJ67, Q9UNC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2002
Last modified: September 3, 2014
This is version 169 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Physiological concentrations of calcium ions negatively affect the assembly of apoptosome by inhibiting nucleotide exchange in the monomeric form.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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