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O14713 (ITBP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Integrin beta-1-binding protein 1
Alternative name(s):
Integrin cytoplasmic domain-associated protein 1
Short name=ICAP-1
Gene names
Name:ITGB1BP1
Synonyms:ICAP1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length200 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulates integrin signaling by binding to the ITGB1 cytoplasmic tail and preventing the activation of integrin alpha-5/beta-1 (heterodimer of ITGA5 and ITGB1) by talin or FERMT1. May play a role in the recruitment of ITGB1 to focal contacts during integrin-dependent cell adhesion. Ref.8

Subunit structure

Interacts with KRIT1 and ITGB1; KRIT1 and ITGB1 compete for the same binding site. Isoform 2 does not interact with ITGB1. Ref.1 Ref.6 Ref.8

Subcellular location

Cytoplasm.

Tissue specificity

Expressed in intestine, colon, testis, ovary, thymus, spleen and prostate. Ref.1

Post-translational modification

Phosphorylation at Thr-38 seems to enhance integrin alpha5beta1-mediated cell adhesion. The degree of phosphorylation is regulated by integrin-dependent cell-matrix interaction.

Sequence similarities

Contains 1 PID domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

NME2P223927EBI-2127367,EBI-713693

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O14713-1)

Also known as: ICAP1-alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Isoform 2 (identifier: O14713-2)

Also known as: ICAP1-beta;

The sequence of this isoform differs from the canonical sequence as follows:
     128-177: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 200200Integrin beta-1-binding protein 1
PRO_0000084264

Regions

Domain58 – 200143PID
Region136 – 1394Interaction with KRIT1
Region139 – 1413Interaction with ITGB1
Compositional bias10 – 5748Ser/Thr-rich

Amino acid modifications

Modified residue381Phosphothreonine; by CaMK2 Ref.5

Natural variations

Alternative sequence128 – 17750Missing in isoform 2.
VSP_003898

Experimental info

Mutagenesis381T → A: Stimulates cell spreading on fibronectin to a similar extent as inhibition of CaMKII. Ref.5 Ref.6
Mutagenesis381T → D: Changes in cell spreading. Ref.5 Ref.6
Mutagenesis381T → D: Strong defect in cell spreading. Ref.5 Ref.6
Mutagenesis821L → A: Reduces ITGB1 binding. Ref.6
Mutagenesis821L → Q: No effect on ITGB1 binding. Ref.6
Mutagenesis861L → Q: No effect on ITGB1 binding. Ref.6
Mutagenesis891I → R: Reduces KRIT1 binding. No effect on ITGB1 binding. Ref.8
Mutagenesis931D → A: Abolishes KRIT1 binding; when associated with A-96. Ref.8
Mutagenesis961Q → A: Abolishes KRIT1 binding; when associated with A-93. Ref.8
Mutagenesis135 – 1395LYLII → AYAA: Reduces KRIT1 and ITGB1 binding. Ref.6 Ref.8
Mutagenesis1351L → A: Abolishes ITGB1 binding. Ref.6
Mutagenesis1381I → A: Abolishes ITGB1 binding. Ref.6
Mutagenesis1391I → A: Abolishes ITGB1 binding. Ref.6
Mutagenesis1441Y → T: Abolishes ITGB1 binding. Ref.6
Mutagenesis1841C → D: Reduces KRIT1 and ITGB1 binding. Ref.8
Sequence conflict1501A → V in AAH12264. Ref.4

Secondary structure

...................... 200
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (ICAP1-alpha) [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 0F041238E68FBE23

FASTA20021,782
        10         20         30         40         50         60 
MFRKGKKRHS SSSSQSSEIS TKSKSVDSSL GGLSRSSTVA SLDTDSTKSS GQSNNNSDTC 

        70         80         90        100        110        120 
AEFRIKYVGA IEKLKLSEGK GLEGPLDLIN YIDVAQQDGK LPFVPPEEEF IMGVSKYGIK 

       130        140        150        160        170        180 
VSTSDQYDVL HRHALYLIIR MVCYDDGLGA GKSLLALKTT DASNEEYSLW VYQCNSLEQA 

       190        200 
QAICKVLSTA FDSVLTSEKP 

« Hide

Isoform 2 (ICAP1-beta) [UniParc].

Checksum: FC6E3D387878DB81
Show »

FASTA15016,140

References

« Hide 'large scale' references
[1]"ICAP-1, a novel beta1 integrin cytoplasmic domain-associated protein, binds to a conserved and functionally important NPXY sequence motif of beta1 integrin."
Chang D.D., Wong C., Smith H., Liu J.
J. Cell Biol. 138:1149-1157(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INTERACTION WITH ITGB1, PHOSPHORYLATION, TISSUE SPECIFICITY.
Tissue: Cervix carcinoma.
[2]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Ovary.
[5]"Calcium/calmodulin-dependent protein kinase II controls integrin alpha5beta1-mediated cell adhesion through the integrin cytoplasmic domain associated protein-1alpha."
Bouvard D., Block M.R.
Biochem. Biophys. Res. Commun. 252:46-50(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT THR-38, MUTAGENESIS OF THR-38.
[6]"Molecular basis for interaction between Icap1alpha PTB domain and beta 1 integrin."
Chang D.D., Hoang B.Q., Liu J., Springer T.A.
J. Biol. Chem. 277:8140-8145(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ITGB1, MUTAGENESIS OF THR-38; LEU-82; LEU-86; LEU-135; ILE-138; ILE-139 AND TYR-144, 3D-STRUCTURE MODELING.
[7]"An unappreciated role for RNA surveillance."
Hillman R.T., Green R.E., Brenner S.E.
Genome Biol. 5:R8.1-R8.16(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
[8]"Mechanism for KRIT1 release of ICAP1-mediated suppression of integrin activation."
Liu W., Draheim K.M., Zhang R., Calderwood D.A., Boggon T.J.
Mol. Cell 0:0-0(2013)
Cited for: X-RAY CRYSTALLOGRAPHY (2.54 ANGSTROMS) OF 49-200 IN COMPLEXES WITH KRIT1 AND ITGB1, FUNCTION, INTERACTION WITH KRIT1 AND ITGB1, MUTAGENESIS OF ILE-89; ASP-93; GLN-96; 135-LEU--ILE-139 AND CYS-184.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF012023 mRNA. Translation: AAB88671.1.
AF012024 mRNA. Translation: AAB88672.1.
CH471053 Genomic DNA. Translation: EAX00992.1.
CH471053 Genomic DNA. Translation: EAX00995.1.
CH471053 Genomic DNA. Translation: EAX01000.1.
CH471053 Genomic DNA. Translation: EAX01001.1.
AC080162 Genomic DNA. Translation: AAY14857.1.
CH471053 Genomic DNA. Translation: EAX00993.1.
CH471053 Genomic DNA. Translation: EAX00997.1.
BC012264 mRNA. Translation: AAH12264.1.
IPIIPI00006265.
IPI00219566.
RefSeqNP_004754.1. NM_004763.3.
NP_071729.1. NM_022334.3.
UniGeneHs.467662.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1K11model-A58-196[»]
4DX8X-ray2.54A/B/D/E49-200[»]
4DX9X-ray3.000/1/2/3/4/5/A/C/E/G/I/K/M/O/Q/S/U/W/Y/a/c/e/g/i/k/m/o/q/s/u/w/y49-200[»]
ProteinModelPortalO14713.
ModBaseSearch...

Protein-protein interaction databases

IntActO14713. 5 interactions.
STRING9606.ENSP00000347504.

PTM databases

PhosphoSiteO14713.

Proteomic databases

PaxDbO14713.
PRIDEO14713.

Protocols and materials databases

DNASU9270.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000238091; ENSP00000238091; ENSG00000119185.
ENST00000355346; ENSP00000347504; ENSG00000119185.
ENST00000360635; ENSP00000353850; ENSG00000119185.
ENST00000488451; ENSP00000419524; ENSG00000119185.
GeneID9270.
KEGGhsa:9270.
UCSCuc002qzj.3. human.
uc002qzk.3. human.

Organism-specific databases

CTD9270.
GeneCardsGC02M009496.
HGNCHGNC:23927. ITGB1BP1.
MIM607153. gene.
neXtProtNX_O14713.
PharmGKBPA134913590.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG45114.
HOGENOMHOG000015089.
HOVERGENHBG052155.
InParanoidO14713.
OMAVLHRHPL.
OrthoDBEOG483D5W.
PhylomeDBO14713.

Gene expression databases

ArrayExpressO14713.
BgeeO14713.
CleanExHS_ITGB1BP1.
GenevestigatorO14713.
GermOnlineENSG00000119185. Homo sapiens.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR019517. Integrin-bd_ICAP-1.
IPR011993. PH_like_dom.
IPR006020. PTyr_interaction_dom.
[Graphical view]
PfamPF10480. ICAP-1_inte_bdg. 1 hit.
[Graphical view]
SMARTSM00462. PTB. 1 hit.
[Graphical view]
PROSITEPS01179. PID. False negative.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSITGB1BP1. human.
GenomeRNAi9270.
NextBio34745.
SOURCESearch...

Entry information

Entry nameITBP1_HUMAN
AccessionPrimary (citable) accession number: O14713
Secondary accession number(s): D6W4Y9, O14714, Q53RS0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: January 1, 1998
Last modified: April 3, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families