O14713 (ITBP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 101.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Integrin beta-1-binding protein 1 Alternative name(s): Integrin cytoplasmic domain-associated protein 1 Short name=ICAP-1 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 200 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | May play a role in the recruitment of beta-1 integrins to the focal contacts during integrin-dependent cell adhesion. Isoform 2 does not bind the integrin cytoplasmic domain-associated protein-1. |
| Subunit structure | Interacts specifically with the beta-1 integrin cytoplasmic domain-associated protein-1. |
| Subcellular location | |
| Tissue specificity | Expressed in intestine, colon, testis, ovary, thymus, spleen and prostate. |
| Post-translational modification | Isoform 1 appears to be phosphorylated. The degree of phosphorylation is regulated by integrin-dependent cell-matrix interaction. Ref.7 |
| Sequence similarities | Contains 1 PID domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm |
| Coding sequence diversity | Alternative splicing |
| PTM | Phosphoprotein |
| Technical term | 3D-structure Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | cell migration Traceable author statement. Source: HGNC cell-matrix adhesionInferred from direct assay Ref.1. Source: UniProtKB intracellular protein kinase cascadeTraceable author statement. Source: ProtInc |
| Cellular component | cytosol Inferred from direct assay. Source: HGNC lamellipodiumInferred from direct assay. Source: HGNC membraneNon-traceable author statement Ref.1. Source: UniProtKB ruffleInferred from direct assay. Source: HGNC |
| Molecular function | protein binding Inferred from physical interaction Ref.1. Source: UniProtKB |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| NME2 | P22392 | 7 | EBI-2127367,EBI-713693 |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: O14713-1) Also known as: ICAP1-alpha; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. | ||||||
| Isoform 2 (identifier: O14713-2) Also known as: ICAP1-beta; The sequence of this isoform differs from the canonical sequence as follows: 128-177: Missing. | ||||||
| Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||||||||||
Molecule processing | ||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 200 | 200 | Integrin beta-1-binding protein 1 | PRO_0000084264 | ||||||||||||||||||||||||||
Regions | ||||||||||||||||||||||||||||||
| Domain | 58 – 200 | 143 | PID | |||||||||||||||||||||||||||
| Compositional bias | 10 – 57 | 48 | Ser/Thr-rich | |||||||||||||||||||||||||||
Amino acid modifications | ||||||||||||||||||||||||||||||
| Modified residue | 41 | 1 | Phosphoserine Ref.7 | |||||||||||||||||||||||||||
Natural variations | ||||||||||||||||||||||||||||||
| Alternative sequence | 128 – 177 | 50 | Missing in isoform 2. | VSP_003898 | ||||||||||||||||||||||||||
Experimental info | ||||||||||||||||||||||||||||||
| Mutagenesis | 38 | 1 | T → D: Changes in cell spreading. Ref.5 | |||||||||||||||||||||||||||
| Mutagenesis | 82 | 1 | L → A: Decrease in binding to beta 1 integrin; when associated with T-144 no binding to beta 1 integrin. Ref.5 | |||||||||||||||||||||||||||
| Mutagenesis | 82 | 1 | L → Q: No change in binding to beta 1 integrin. Ref.5 | |||||||||||||||||||||||||||
| Mutagenesis | 86 | 1 | L → Q: No change in binding to beta 1 integrin; when associated with T-144 no binding to beta 1 integrin. Ref.5 | |||||||||||||||||||||||||||
| Mutagenesis | 135 | 1 | L → A: No binding to beta 1 integrin. Ref.5 | |||||||||||||||||||||||||||
| Mutagenesis | 138 | 1 | I → A: No binding to beta 1 integrin. Ref.5 | |||||||||||||||||||||||||||
| Mutagenesis | 139 | 1 | I → A: No binding to beta 1 integrin. Ref.5 | |||||||||||||||||||||||||||
| Mutagenesis | 144 | 1 | Y → T: No binding to beta 1 integrin. Ref.5 | |||||||||||||||||||||||||||
| Sequence conflict | 150 | 1 | A → V in AAH12264. Ref.4 | |||||||||||||||||||||||||||
Secondary structure | ||||||||||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||||||||||
| Beta strand | 61 – 73 | 13 | ||||||||||||||||||||||||||||
| Helix | 80 – 97 | 18 | ||||||||||||||||||||||||||||
| Beta strand | 109 – 114 | 6 | ||||||||||||||||||||||||||||
| Beta strand | 116 – 123 | 8 | ||||||||||||||||||||||||||||
| Turn | 124 – 126 | 3 | ||||||||||||||||||||||||||||
| Beta strand | 129 – 134 | 6 | ||||||||||||||||||||||||||||
| Turn | 135 – 137 | 3 | ||||||||||||||||||||||||||||
| Beta strand | 138 – 144 | 7 | ||||||||||||||||||||||||||||
| Beta strand | 154 – 160 | 7 | ||||||||||||||||||||||||||||
| Beta strand | 162 – 165 | 4 | ||||||||||||||||||||||||||||
| Beta strand | 167 – 174 | 8 | ||||||||||||||||||||||||||||
| Helix | 176 – 193 | 18 | ||||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "ICAP-1, a novel beta1 integrin cytoplasmic domain-associated protein, binds to a conserved and functionally important NPXY sequence motif of beta1 integrin." Chang D.D., Wong C., Smith H., Liu J. J. Cell Biol. 138:1149-1157(1997) [PubMed: 9281591] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Cervix carcinoma. |
| [2] | "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. Wilson R.K.Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Ovary. |
| [5] | "Molecular basis for interaction between Icap1alpha PTB domain and beta 1 integrin." Chang D.D., Hoang B.Q., Liu J., Springer T.A. J. Biol. Chem. 277:8140-8145(2002) [PubMed: 11741908] [Abstract] Cited for: MUTAGENESIS OF THR-38; LEU-82; LEU-86; LEU-135; ILE-138; ILE-139 AND TYR-144, 3D-STRUCTURE MODELING. |
| [6] | "An unappreciated role for RNA surveillance." Hillman R.T., Green R.E., Brenner S.E. Genome Biol. 5:R8.1-R8.16(2004) [PubMed: 14759258] [Abstract] Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S). |
| [7] | "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41, MASS SPECTROMETRY. Tissue: Leukemic T-cell. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | AF012023 mRNA. Translation: AAB88671.1. AF012024 mRNA. Translation: AAB88672.1. CH471053 Genomic DNA. Translation: EAX00992.1. CH471053 Genomic DNA. Translation: EAX00995.1. CH471053 Genomic DNA. Translation: EAX01000.1. CH471053 Genomic DNA. Translation: EAX01001.1. AC080162 Genomic DNA. Translation: AAY14857.1. CH471053 Genomic DNA. Translation: EAX00993.1. CH471053 Genomic DNA. Translation: EAX00997.1. BC012264 mRNA. Translation: AAH12264.1. | ||||||||||||
| IPI | IPI00006265. IPI00219566. | ||||||||||||
| RefSeq | NP_004754.1. NM_004763.3. NP_071729.1. NM_022334.3. | ||||||||||||
| UniGene | Hs.467662. | ||||||||||||
3D structure databases | |||||||||||||
| PDBe RCSB PDB PDBj |
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| ProteinModelPortal | O14713. | ||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| IntAct | O14713. 2 interactions. | ||||||||||||
| STRING | O14713. | ||||||||||||
PTM databases | |||||||||||||
| PhosphoSite | O14713. | ||||||||||||
Proteomic databases | |||||||||||||
| PRIDE | O14713. | ||||||||||||
Protocols and materials databases | |||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENST00000355346; ENSP00000347504; ENSG00000119185. ENST00000360635; ENSP00000353850; ENSG00000119185. | ||||||||||||
| GeneID | 9270. | ||||||||||||
| KEGG | hsa:9270. | ||||||||||||
| UCSC | uc002qzj.1. human. uc002qzk.1. human. | ||||||||||||
Organism-specific databases | |||||||||||||
| CTD | 9270. | ||||||||||||
| GeneCards | GC02M009496. | ||||||||||||
| H-InvDB | HIX0001808. | ||||||||||||
| HGNC | HGNC:23927. ITGB1BP1. | ||||||||||||
| MIM | 607153. gene. | ||||||||||||
| neXtProt | NX_O14713. | ||||||||||||
| PharmGKB | PA134913590. | ||||||||||||
| GenAtlas | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| eggNOG | prNOG10677. | ||||||||||||
| HOGENOM | HBG713132. | ||||||||||||
| HOVERGEN | HBG052155. | ||||||||||||
| InParanoid | O14713. | ||||||||||||
| OMA | LDTCAEF. | ||||||||||||
| OrthoDB | EOG483D5W. | ||||||||||||
| PhylomeDB | O14713. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | O14713. | ||||||||||||
| Bgee | O14713. | ||||||||||||
| CleanEx | HS_ITGB1BP1. | ||||||||||||
| Genevestigator | O14713. | ||||||||||||
| GermOnline | ENSG00000119185. Homo sapiens. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR019517. Integrin-bd_ICAP-1. IPR011993. PH_type. IPR006020. PTyr_interaction_dom. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:2.30.29.30. PH_type. 1 hit. | ||||||||||||
| Pfam | PF10480. ICAP-1_inte_bdg. 1 hit. [Graphical view] | ||||||||||||
| SMART | SM00462. PTB. 1 hit. [Graphical view] | ||||||||||||
| PROSITE | PS01179. PID. False negative. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other | |||||||||||||
| NextBio | 34745. | ||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | ITBP1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: O14713 Secondary accession number(s): D6W4Y9, O14714, Q53RS0 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 2 Human chromosome 2: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

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