O14713 (ITBP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Integrin beta-1-binding protein 1

Alternative name(s):
Integrin cytoplasmic domain-associated protein 1
Short name=ICAP-1

Gene names

Name:	ITGB1BP1
Synonyms:	ICAP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in the recruitment of beta-1 integrins to the focal contacts during integrin-dependent cell adhesion. Isoform 2 does not bind the integrin cytoplasmic domain-associated protein-1.
Subunit structure	Interacts specifically with the beta-1 integrin cytoplasmic domain-associated protein-1.
Subcellular location	Cytoplasm.
Tissue specificity	Expressed in intestine, colon, testis, ovary, thymus, spleen and prostate.
Post-translational modification	Isoform 1 appears to be phosphorylated. The degree of phosphorylation is regulated by integrin-dependent cell-matrix interaction. Ref.7
Sequence similarities	Contains 1 PID domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell migration Traceable author statement. Source: HGNC cell-matrix adhesion Inferred from direct assay Ref.1. Source: UniProtKB intracellular protein kinase cascade Traceable author statement. Source: ProtInc
Cellular component	cytosol Inferred from direct assay. Source: HGNC lamellipodium Inferred from direct assay. Source: HGNC membrane Non-traceable author statement Ref.1. Source: UniProtKB ruffle Inferred from direct assay. Source: HGNC
Molecular function	protein binding Inferred from physical interaction Ref.1. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NME2	P22392	7	EBI-2127367,EBI-713693

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O14713-1)

Also known as: ICAP1-alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Isoform 2 (identifier: O14713-2)

Also known as: ICAP1-beta;

The sequence of this isoform differs from the canonical sequence as follows:
     128-177: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 200

200

Integrin beta-1-binding protein 1

PRO_0000084264

Regions

Domain

58 – 200

143

PID

Compositional bias

10 – 57

48

Ser/Thr-rich

Amino acid modifications

Modified residue

41

1

Phosphoserine Ref.7

Natural variations

Alternative sequence

128 – 177

50

Missing in isoform 2.

VSP_003898

Experimental info

Mutagenesis

38

1

T → D: Changes in cell spreading. Ref.5

Mutagenesis

82

1

L → A: Decrease in binding to beta 1 integrin; when associated with T-144 no binding to beta 1 integrin. Ref.5

Mutagenesis

82

1

L → Q: No change in binding to beta 1 integrin. Ref.5

Mutagenesis

86

1

L → Q: No change in binding to beta 1 integrin; when associated with T-144 no binding to beta 1 integrin. Ref.5

Mutagenesis

135

1

L → A: No binding to beta 1 integrin. Ref.5

Mutagenesis

138

1

I → A: No binding to beta 1 integrin. Ref.5

Mutagenesis

139

1

I → A: No binding to beta 1 integrin. Ref.5

Mutagenesis

144

1

Y → T: No binding to beta 1 integrin. Ref.5

Sequence conflict

150

1

A → V in AAH12264. Ref.4

Secondary structure

1

.

.

.

.

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.

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.

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200

Helix Strand Turn

Details...

Beta strand

61 – 73

13

Helix

80 – 97

18

Beta strand

109 – 114

6

Beta strand

116 – 123

8

Turn

124 – 126

3

Beta strand

129 – 134

6

Turn

135 – 137

3

Beta strand

138 – 144

7

Beta strand

154 – 160

7

Beta strand

162 – 165

4

Beta strand

167 – 174

8

Helix

176 – 193

18

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (ICAP1-alpha) [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 0F041238E68FBE23	FASTA	200	21,782
	10 20 30 40 50 60 MFRKGKKRHS SSSSQSSEIS TKSKSVDSSL GGLSRSSTVA SLDTDSTKSS GQSNNNSDTC 70 80 90 100 110 120 AEFRIKYVGA IEKLKLSEGK GLEGPLDLIN YIDVAQQDGK LPFVPPEEEF IMGVSKYGIK 130 140 150 160 170 180 VSTSDQYDVL HRHALYLIIR MVCYDDGLGA GKSLLALKTT DASNEEYSLW VYQCNSLEQA 190 200 QAICKVLSTA FDSVLTSEKP
	Isoform 2 (ICAP1-beta) [UniParc]. Checksum: FC6E3D387878DB81	FASTA	150	16,140
	10 20 30 40 50 60 MFRKGKKRHS SSSSQSSEIS TKSKSVDSSL GGLSRSSTVA SLDTDSTKSS GQSNNNSDTC 70 80 90 100 110 120 AEFRIKYVGA IEKLKLSEGK GLEGPLDLIN YIDVAQQDGK LPFVPPEEEF IMGVSKYGIK 130 140 150 VSTSDQYEQA QAICKVLSTA FDSVLTSEKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ICAP-1, a novel beta1 integrin cytoplasmic domain-associated protein, binds to a conserved and functionally important NPXY sequence motif of beta1 integrin." Chang D.D., Wong C., Smith H., Liu J. J. Cell Biol. 138:1149-1157(1997) [PubMed: 9281591] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Cervix carcinoma.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Ovary.
[5]	"Molecular basis for interaction between Icap1alpha PTB domain and beta 1 integrin." Chang D.D., Hoang B.Q., Liu J., Springer T.A. J. Biol. Chem. 277:8140-8145(2002) [PubMed: 11741908] [Abstract] Cited for: MUTAGENESIS OF THR-38; LEU-82; LEU-86; LEU-135; ILE-138; ILE-139 AND TYR-144, 3D-STRUCTURE MODELING.
[6]	"An unappreciated role for RNA surveillance." Hillman R.T., Green R.E., Brenner S.E. Genome Biol. 5:R8.1-R8.16(2004) [PubMed: 14759258] [Abstract] Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF012023 mRNA. Translation: AAB88671.1.
AF012024 mRNA. Translation: AAB88672.1.
CH471053 Genomic DNA. Translation: EAX00992.1.
CH471053 Genomic DNA. Translation: EAX00995.1.
CH471053 Genomic DNA. Translation: EAX01000.1.
CH471053 Genomic DNA. Translation: EAX01001.1.
AC080162 Genomic DNA. Translation: AAY14857.1.
CH471053 Genomic DNA. Translation: EAX00993.1.
CH471053 Genomic DNA. Translation: EAX00997.1.
BC012264 mRNA. Translation: AAH12264.1.

IPI

IPI00006265.
IPI00219566.

RefSeq

NP_004754.1. NM_004763.3.
NP_071729.1. NM_022334.3.

UniGene

Hs.467662.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K11	model	-	A	58-196	[»]

ProteinModelPortal

O14713.

ModBase

Search...

Protein-protein interaction databases

IntAct

O14713. 2 interactions.

STRING

O14713.

PTM databases

PhosphoSite

O14713.

Proteomic databases

PRIDE

O14713.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000355346; ENSP00000347504; ENSG00000119185.
ENST00000360635; ENSP00000353850; ENSG00000119185.

GeneID

9270.

KEGG

hsa:9270.

UCSC

uc002qzj.1. human.
uc002qzk.1. human.

Organism-specific databases

CTD

9270.

GeneCards

GC02M009496.

H-InvDB

HIX0001808.

HGNC

HGNC:23927. ITGB1BP1.

MIM

607153. gene.

neXtProt

NX_O14713.

PharmGKB

PA134913590.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG10677.

HOGENOM

HBG713132.

HOVERGEN

HBG052155.

InParanoid

O14713.

OMA

LDTCAEF.

OrthoDB

EOG483D5W.

PhylomeDB

O14713.

Gene expression databases

ArrayExpress

O14713.

Bgee

O14713.

CleanEx

HS_ITGB1BP1.

Genevestigator

O14713.

GermOnline

ENSG00000119185. Homo sapiens.

Family and domain databases

InterPro

IPR019517. Integrin-bd_ICAP-1.
IPR011993. PH_type.
IPR006020. PTyr_interaction_dom.
[Graphical view]

Gene3D

G3DSA:2.30.29.30. PH_type. 1 hit.

Pfam

PF10480. ICAP-1_inte_bdg. 1 hit.
[Graphical view]

SMART

SM00462. PTB. 1 hit.
[Graphical view]

PROSITE

PS01179. PID. False negative.
[Graphical view]

ProtoNet

Search...

Other

NextBio

34745.

SOURCE

Search...

Entry information

Entry name

ITBP1_HUMAN

Accession

Primary (citable) accession number: O14713
Secondary accession number(s): D6W4Y9, O14714, Q53RS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.