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O14681

- EI24_HUMAN

UniProt

O14681 - EI24_HUMAN

Protein

Etoposide-induced protein 2.4 homolog

Gene

EI24

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 4 (05 May 2009)
      Previous versions | rss
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    Functioni

    Acts as a negative growth regulator via p53-mediated apoptosis pathway. Regulates formation of degradative autolysosomes during autophagy By similarity.By similarity

    GO - Biological processi

    1. apoptotic process Source: ProtInc
    2. autophagy Source: UniProtKB-KW
    3. intrinsic apoptotic signaling pathway in response to DNA damage Source: Ensembl
    4. negative regulation of cell growth Source: MGI
    5. neuromuscular process controlling balance Source: Ensembl
    6. positive regulation of intrinsic apoptotic signaling pathway Source: Ensembl
    7. response to drug Source: Ensembl

    Keywords - Biological processi

    Apoptosis, Autophagy

    Protein family/group databases

    TCDBi9.B.7.3.1. the putative sulfate transporter (cysz) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Etoposide-induced protein 2.4 homolog
    Alternative name(s):
    p53-induced gene 8 protein
    Gene namesi
    Name:EI24
    Synonyms:PIG8
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:13276. EI24.

    Subcellular locationi

    Nucleus membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cytoplasm 1 Publication. Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. membrane Source: UniProtKB
    4. nuclear membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

    Pathology & Biotechi

    Involvement in diseasei

    EI24 is on a chromosomal region frequently deleted in solid tumors, and it is thought to play a role in breast and cervical cancer. Particularly, expression analysis of EI24 in cancerous tissues shows that EI24 loss is associated with tumor invasiveness.

    Keywords - Diseasei

    Disease mutation

    Organism-specific databases

    PharmGKBiPA134937367.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 340339Etoposide-induced protein 2.4 homologPRO_0000086945Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Modified residuei56 – 561Phosphoserine2 Publications
    Modified residuei326 – 3261Phosphoserine2 Publications
    Modified residuei330 – 3301Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiO14681.
    PaxDbiO14681.
    PRIDEiO14681.

    PTM databases

    PhosphoSiteiO14681.

    Expressioni

    Inductioni

    By p53/TP53.1 Publication

    Gene expression databases

    ArrayExpressiO14681.
    BgeeiO14681.
    CleanExiHS_EI24.
    GenevestigatoriO14681.

    Organism-specific databases

    HPAiHPA047165.

    Interactioni

    Subunit structurei

    Interacts with BCL2.By similarity

    Protein-protein interaction databases

    BioGridi114914. 2 interactions.
    IntActiO14681. 2 interactions.
    STRINGi9606.ENSP00000278903.

    Structurei

    3D structure databases

    ProteinModelPortaliO14681.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei77 – 9721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei117 – 13721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei179 – 19921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei238 – 25518HelicalSequence AnalysisAdd
    BLAST
    Transmembranei257 – 27721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi41 – 444Poly-Arg

    Sequence similaritiesi

    Belongs to the EI24 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG266932.
    HOGENOMiHOG000293197.
    HOVERGENiHBG001857.
    InParanoidiO14681.
    KOiK10134.
    PhylomeDBiO14681.
    TreeFamiTF314441.

    Family and domain databases

    InterProiIPR009890. EI24.
    [Graphical view]
    PANTHERiPTHR21389. PTHR21389. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O14681-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MADSVKTFLQ DLARGIKDSI WGICTISKLD ARIQQKREEQ RRRRASSVLA    50
    QRRAQSIERK QESEPRIVSR IFQCCAWNGG VFWFSLLLFY RVFIPVLQSV 100
    TARIIGDPSL HGDVWSWLEF FLTSIFSALW VLPLFVLSKV VNAIWFQDIA 150
    DLAFEVSGRK PHPFPSVSKI IADMLFNLLL QALFLIQGMF VSLFPIHLVG 200
    QLVSLLHMSL LYSLYCFEYR WFNKGIEMHQ RLSNIERNWP YYFGFGLPLA 250
    FLTAMQSSYI ISGCLFSILF PLFIISANEA KTPGKAYLFQ LRLFSLVVFL 300
    SNRLFHKTVY LQSALSSSTS AEKFPSPHPS PAKLKATAGH 340
    Length:340
    Mass (Da):38,965
    Last modified:May 5, 2009 - v4
    Checksum:i928DB15D9B34146E
    GO
    Isoform 2 (identifier: O14681-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         15-28: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:326
    Mass (Da):37,462
    Checksum:iEFC6A2CA5E6B46E5
    GO

    Sequence cautioni

    The sequence AAC39531.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti54 – 541A → P in AAC39531. (PubMed:9305847)Curated
    Sequence conflicti119 – 1191E → G in AAC39531. (PubMed:9305847)Curated
    Sequence conflicti129 – 1291L → V in AAC39531. (PubMed:9305847)Curated
    Sequence conflicti132 – 1321L → V in BAF84640. (PubMed:14702039)Curated
    Sequence conflicti254 – 2541A → S in BAG59228. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti30 – 301D → G in some patients with early onset breast cancer. 1 Publication
    VAR_065459
    Natural varianti195 – 1951P → W in some patients with early onset breast cancer; requires 2 nucleotide substitutions. 1 Publication
    VAR_065460
    Natural varianti196 – 1961I → D in some patients with early onset breast cancer; requires 2 nucleotide substitutions. 1 Publication
    VAR_065461
    Natural varianti197 – 1971H → Y in some patients with early onset breast cancer. 1 Publication
    VAR_065462
    Natural varianti199 – 1991V → H in some patients with early onset breast cancer; requires 2 nucleotide substitutions. 1 Publication
    VAR_065463
    Natural varianti319 – 3191T → A in some patients with early onset breast cancer. 1 Publication
    VAR_065464

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei15 – 2814Missing in isoform 2. 1 PublicationVSP_055466Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF010313 mRNA. Translation: AAC39531.2. Different initiation.
    AK291951 mRNA. Translation: BAF84640.1.
    AK296620 mRNA. Translation: BAG59228.1.
    AK315841 mRNA. Translation: BAF98732.1.
    CH471065 Genomic DNA. Translation: EAW67641.1.
    BC002390 mRNA. Translation: AAH02390.1.
    RefSeqiNP_001277064.1. NM_001290135.1.
    NP_004870.3. NM_004879.4.
    UniGeneiHs.643514.

    Genome annotation databases

    EnsembliENST00000278903; ENSP00000278903; ENSG00000149547.
    GeneIDi9538.
    KEGGihsa:9538.
    UCSCiuc001qca.3. human.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF010313 mRNA. Translation: AAC39531.2 . Different initiation.
    AK291951 mRNA. Translation: BAF84640.1 .
    AK296620 mRNA. Translation: BAG59228.1 .
    AK315841 mRNA. Translation: BAF98732.1 .
    CH471065 Genomic DNA. Translation: EAW67641.1 .
    BC002390 mRNA. Translation: AAH02390.1 .
    RefSeqi NP_001277064.1. NM_001290135.1.
    NP_004870.3. NM_004879.4.
    UniGenei Hs.643514.

    3D structure databases

    ProteinModelPortali O14681.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114914. 2 interactions.
    IntActi O14681. 2 interactions.
    STRINGi 9606.ENSP00000278903.

    Protein family/group databases

    TCDBi 9.B.7.3.1. the putative sulfate transporter (cysz) family.

    PTM databases

    PhosphoSitei O14681.

    Proteomic databases

    MaxQBi O14681.
    PaxDbi O14681.
    PRIDEi O14681.

    Protocols and materials databases

    DNASUi 9538.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000278903 ; ENSP00000278903 ; ENSG00000149547 .
    GeneIDi 9538.
    KEGGi hsa:9538.
    UCSCi uc001qca.3. human.

    Organism-specific databases

    CTDi 9538.
    GeneCardsi GC11P125441.
    H-InvDB HIX0010241.
    HGNCi HGNC:13276. EI24.
    HPAi HPA047165.
    MIMi 605170. gene.
    neXtProti NX_O14681.
    PharmGKBi PA134937367.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG266932.
    HOGENOMi HOG000293197.
    HOVERGENi HBG001857.
    InParanoidi O14681.
    KOi K10134.
    PhylomeDBi O14681.
    TreeFami TF314441.

    Miscellaneous databases

    ChiTaRSi EI24. human.
    GeneWikii EI24.
    GenomeRNAii 9538.
    NextBioi 35764.
    PROi O14681.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O14681.
    Bgeei O14681.
    CleanExi HS_EI24.
    Genevestigatori O14681.

    Family and domain databases

    InterProi IPR009890. EI24.
    [Graphical view ]
    PANTHERi PTHR21389. PTHR21389. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INDUCTION BY TP53.
      Tissue: Colon cancer.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Colon and Synovium.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Lung.
    5. "Candidate tumour suppressor genes at 11q23-q24 in breast cancer: evidence of alterations in PIG8, a gene involved in p53-induced apoptosis."
      Gentile M., Ahnstrom M., Schon F., Wingren S.
      Oncogene 20:7753-7760(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: POSSIBLE INVOLVEMENT IN BREAST CANCER, VARIANTS GLY-30; TRP-195; ASP-196; TYR-197; HIS-199 AND ALA-319.
    6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326 AND SER-330, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Inactivation of CHEK1 and EI24 are associated with the development of invasive cervical carcinoma: Clinical and prognostic implications."
      Mazumder ' Indra ' D., Mitra S., Singh R.K., Dutta S., Roy A., Mondal R.K., Basu P.S., Roychoudhury S., Panda C.K.
      Int. J. Cancer 129:1859-1871(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, POSSIBLE INVOLVEMENT IN CERVICAL CANCER.
    13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiEI24_HUMAN
    AccessioniPrimary (citable) accession number: O14681
    Secondary accession number(s): A8K7D6, B4DKL6, Q9BUQ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 112 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3