O14681 (EI24_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Etoposide-induced protein 2.4 homolog

Alternative name(s):
p53-induced gene 8 protein

Gene names

Name:	EI24
Synonyms:	PIG8

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a negative growth regulator via p53-mediated apoptosis pathway. Regulates formation of degradative autolysosomes during autophagy By similarity.
Subunit structure	Interacts with BCL2 By similarity.
Subcellular location	Nucleus membrane; Multi-pass membrane protein. Cytoplasm. Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity Ref.12.
Induction	By p53/TP53. Ref.1
Involvement in disease	EI24 is on a chromosomal region frequently deleted in solid tumors, and it is thought to play a role in breast and cervical cancer. Particularly, expression analysis of EI24 in cancerous tissues shows that EI24 loss is associated with tumor invasiveness.
Sequence similarities	Belongs to the EI24 family.
Sequence caution	The sequence AAC39531.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Apoptosis Autophagy
Cellular component	Cytoplasm Endoplasmic reticulum Membrane Nucleus
Coding sequence diversity	Polymorphism
Disease	Disease mutation
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW autophagy Inferred from electronic annotation. Source: UniProtKB-KW induction of apoptosis Traceable author statement Ref.1. Source: ProtInc negative regulation of cell growth Inferred from direct assay PubMed 10594026. Source: MGI response to drug Inferred from electronic annotation. Source: Compara
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Etoposide-induced protein 2.4 homolog		PRO_0000086945
Regions
	Transmembrane	77 – 97	21	Helical; Potential
	Transmembrane	117 – 137	21	Helical; Potential
	Transmembrane	179 – 199	21	Helical; Potential
	Transmembrane	238 – 255	18	Helical; Potential
	Transmembrane	257 – 277	21	Helical; Potential
	Compositional bias	41 – 44	4	Poly-Arg
Amino acid modifications
	Modified residue	56	1	Phosphoserine Ref.6 Ref.10
	Modified residue	318	1	Phosphoserine By similarity
	Modified residue	326	1	Phosphoserine Ref.7 Ref.8
	Modified residue	330	1	Phosphoserine Ref.8
Natural variations
	Natural variant	30	1	D → G in some patients with early onset breast cancer. Ref.5		VAR_065459
	Natural variant	195	1	P → W in some patients with early onset breast cancer; requires 2 nucleotide substitutions. Ref.5		VAR_065460
	Natural variant	196	1	I → D in some patients with early onset breast cancer; requires 2 nucleotide substitutions. Ref.5		VAR_065461
	Natural variant	197	1	H → Y in some patients with early onset breast cancer. Ref.5		VAR_065462
	Natural variant	199	1	V → H in some patients with early onset breast cancer; requires 2 nucleotide substitutions. Ref.5		VAR_065463
	Natural variant	319	1	T → A in some patients with early onset breast cancer. Ref.5		VAR_065464
Experimental info
	Sequence conflict	54	1	A → P in AAC39531. Ref.1
	Sequence conflict	119	1	E → G in AAC39531. Ref.1
	Sequence conflict	129	1	L → V in AAC39531. Ref.1
	Sequence conflict	132	1	L → V in BAF84640. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O14681 [UniParc]. Last modified May 5, 2009. Version 4. Checksum: 928DB15D9B34146E	FASTA	340	38,965
	10 20 30 40 50 60 MADSVKTFLQ DLARGIKDSI WGICTISKLD ARIQQKREEQ RRRRASSVLA QRRAQSIERK 70 80 90 100 110 120 QESEPRIVSR IFQCCAWNGG VFWFSLLLFY RVFIPVLQSV TARIIGDPSL HGDVWSWLEF 130 140 150 160 170 180 FLTSIFSALW VLPLFVLSKV VNAIWFQDIA DLAFEVSGRK PHPFPSVSKI IADMLFNLLL 190 200 210 220 230 240 QALFLIQGMF VSLFPIHLVG QLVSLLHMSL LYSLYCFEYR WFNKGIEMHQ RLSNIERNWP 250 260 270 280 290 300 YYFGFGLPLA FLTAMQSSYI ISGCLFSILF PLFIISANEA KTPGKAYLFQ LRLFSLVVFL 310 320 330 340 SNRLFHKTVY LQSALSSSTS AEKFPSPHPS PAKLKATAGH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A model for p53-induced apoptosis." Polyak K., Xia Y., Zweier J.L., Kinzler K.W., Vogelstein B. Nature 389:300-306(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION BY TP53. Tissue: Colon cancer.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Synovium.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[5]	"Candidate tumour suppressor genes at 11q23-q24 in breast cancer: evidence of alterations in PIG8, a gene involved in p53-induced apoptosis." Gentile M., Ahnstrom M., Schon F., Wingren S. Oncogene 20:7753-7760(2001) [PubMed] [Europe PMC] [Abstract] Cited for: POSSIBLE INVOLVEMENT IN BREAST CANCER, VARIANTS GLY-30; TRP-195; ASP-196; TYR-197; HIS-199 AND ALA-319.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326 AND SER-330, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[10]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]	"Inactivation of CHEK1 and EI24 are associated with the development of invasive cervical carcinoma: Clinical and prognostic implications." Mazumder ' Indra ' D., Mitra S., Singh R.K., Dutta S., Roy A., Mondal R.K., Basu P.S., Roychoudhury S., Panda C.K. Int. J. Cancer 129:1859-1871(2011) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, POSSIBLE INVOLVEMENT IN CERVICAL CANCER.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF010313 mRNA. Translation: AAC39531.2. Different initiation. AK291951 mRNA. Translation: BAF84640.1. AK315841 mRNA. Translation: BAF98732.1. CH471065 Genomic DNA. Translation: EAW67641.1. BC002390 mRNA. Translation: AAH02390.1.
IPI	IPI00872497.
RefSeq	NP_001007278.1. NM_001007277.1. NP_004870.3. NM_004879.3.
UniGene	Hs.643514.
3D structure databases
ProteinModelPortal	O14681.
ModBase	Search...
Protein-protein interaction databases
IntAct	O14681. 2 interactions.
STRING	9606.ENSP00000278903.
PTM databases
PhosphoSite	O14681.
Proteomic databases
PaxDb	O14681.
PRIDE	O14681.
Protocols and materials databases
DNASU	9538.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000278903; ENSP00000278903; ENSG00000149547.
GeneID	9538.
KEGG	hsa:9538.
UCSC	uc001qca.3. human.
Organism-specific databases
CTD	9538.
GeneCards	GC11P125441.
H-InvDB	HIX0010241.
HGNC	HGNC:13276. EI24.
HPA	HPA047165.
MIM	605170. gene.
neXtProt	NX_O14681.
PharmGKB	PA134937367.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG266932.
HOGENOM	HOG000293197.
HOVERGEN	HBG001857.
InParanoid	O14681.
KO	K10134.
OrthoDB	EOG4JM7QC.
Gene expression databases
ArrayExpress	O14681.
Bgee	O14681.
CleanEx	HS_EI24.
Genevestigator	O14681.
GermOnline	ENSG00000149547. Homo sapiens.
Family and domain databases
InterPro	IPR009890. EI24. [Graphical view]
PANTHER	PTHR21389. PTHR21389. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	EI24. human.
GenomeRNAi	9538.
NextBio	35764.
SOURCE	Search...

Entry information

Entry name

EI24_HUMAN

Accession

Primary (citable) accession number: O14681
Secondary accession number(s): A8K7D6, Q9BUQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 5, 2009
Last modified:	April 3, 2013
This is version 101 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.