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Protein

Etoposide-induced protein 2.4 homolog

Gene

EI24

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a negative growth regulator via p53-mediated apoptosis pathway. Regulates formation of degradative autolysosomes during autophagy (By similarity).By similarity

GO - Biological processi

  • apoptotic process Source: ProtInc
  • autophagy Source: UniProtKB-KW
  • negative regulation of cell growth Source: MGI
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Autophagy

Protein family/group databases

TCDBi2.A.121.3.1. the sulfate transporter (cysz) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Etoposide-induced protein 2.4 homolog
Alternative name(s):
p53-induced gene 8 protein
Gene namesi
Name:EI24
Synonyms:PIG8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:13276. EI24.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei77 – 9721HelicalSequence AnalysisAdd
BLAST
Transmembranei117 – 13721HelicalSequence AnalysisAdd
BLAST
Transmembranei179 – 19921HelicalSequence AnalysisAdd
BLAST
Transmembranei238 – 25518HelicalSequence AnalysisAdd
BLAST
Transmembranei257 – 27721HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Involvement in diseasei

EI24 is on a chromosomal region frequently deleted in solid tumors, and it is thought to play a role in breast and cervical cancer. Particularly, expression analysis of EI24 in cancerous tissues shows that EI24 loss is associated with tumor invasiveness.

Keywords - Diseasei

Disease mutation

Organism-specific databases

PharmGKBiPA134937367.

Polymorphism and mutation databases

BioMutaiEI24.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 340339Etoposide-induced protein 2.4 homologPRO_0000086945Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei56 – 561Phosphoserine2 Publications
Modified residuei326 – 3261Phosphoserine2 Publications
Modified residuei330 – 3301Phosphoserine2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO14681.
PaxDbiO14681.
PRIDEiO14681.

PTM databases

PhosphoSiteiO14681.

Expressioni

Inductioni

By p53/TP53.1 Publication

Gene expression databases

BgeeiO14681.
CleanExiHS_EI24.
ExpressionAtlasiO14681. baseline and differential.
GenevisibleiO14681. HS.

Organism-specific databases

HPAiHPA047165.
HPA051029.

Interactioni

Subunit structurei

Interacts with BCL2.By similarity

Protein-protein interaction databases

IntActiO14681. 2 interactions.
STRINGi9606.ENSP00000278903.

Structurei

3D structure databases

ProteinModelPortaliO14681.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi41 – 444Poly-Arg

Sequence similaritiesi

Belongs to the EI24 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG266932.
GeneTreeiENSGT00390000018633.
HOGENOMiHOG000293197.
HOVERGENiHBG001857.
InParanoidiO14681.
KOiK10134.
OMAiLFHKTVH.
PhylomeDBiO14681.
TreeFamiTF314441.

Family and domain databases

InterProiIPR009890. EI24.
[Graphical view]
PANTHERiPTHR21389. PTHR21389. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O14681-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADSVKTFLQ DLARGIKDSI WGICTISKLD ARIQQKREEQ RRRRASSVLA
60 70 80 90 100
QRRAQSIERK QESEPRIVSR IFQCCAWNGG VFWFSLLLFY RVFIPVLQSV
110 120 130 140 150
TARIIGDPSL HGDVWSWLEF FLTSIFSALW VLPLFVLSKV VNAIWFQDIA
160 170 180 190 200
DLAFEVSGRK PHPFPSVSKI IADMLFNLLL QALFLIQGMF VSLFPIHLVG
210 220 230 240 250
QLVSLLHMSL LYSLYCFEYR WFNKGIEMHQ RLSNIERNWP YYFGFGLPLA
260 270 280 290 300
FLTAMQSSYI ISGCLFSILF PLFIISANEA KTPGKAYLFQ LRLFSLVVFL
310 320 330 340
SNRLFHKTVY LQSALSSSTS AEKFPSPHPS PAKLKATAGH
Length:340
Mass (Da):38,965
Last modified:May 5, 2009 - v4
Checksum:i928DB15D9B34146E
GO
Isoform 2 (identifier: O14681-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     15-28: Missing.

Note: No experimental confirmation available.
Show »
Length:326
Mass (Da):37,462
Checksum:iEFC6A2CA5E6B46E5
GO

Sequence cautioni

The sequence AAC39531.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541A → P in AAC39531 (PubMed:9305847).Curated
Sequence conflicti119 – 1191E → G in AAC39531 (PubMed:9305847).Curated
Sequence conflicti129 – 1291L → V in AAC39531 (PubMed:9305847).Curated
Sequence conflicti132 – 1321L → V in BAF84640 (PubMed:14702039).Curated
Sequence conflicti254 – 2541A → S in BAG59228 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti30 – 301D → G in some patients with early onset breast cancer. 1 Publication
VAR_065459
Natural varianti195 – 1951P → W in some patients with early onset breast cancer; requires 2 nucleotide substitutions. 1 Publication
VAR_065460
Natural varianti196 – 1961I → D in some patients with early onset breast cancer; requires 2 nucleotide substitutions. 1 Publication
VAR_065461
Natural varianti197 – 1971H → Y in some patients with early onset breast cancer. 1 Publication
VAR_065462
Natural varianti199 – 1991V → H in some patients with early onset breast cancer; requires 2 nucleotide substitutions. 1 Publication
VAR_065463
Natural varianti319 – 3191T → A in some patients with early onset breast cancer. 1 Publication
VAR_065464

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei15 – 2814Missing in isoform 2. 1 PublicationVSP_055466Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF010313 mRNA. Translation: AAC39531.2. Different initiation.
AK291951 mRNA. Translation: BAF84640.1.
AK296620 mRNA. Translation: BAG59228.1.
AK315841 mRNA. Translation: BAF98732.1.
CH471065 Genomic DNA. Translation: EAW67641.1.
BC002390 mRNA. Translation: AAH02390.1.
CCDSiCCDS73410.1. [O14681-1]
CCDS76493.1. [O14681-3]
RefSeqiNP_001277064.1. NM_001290135.1. [O14681-3]
NP_004870.3. NM_004879.4. [O14681-1]
XP_011541371.1. XM_011543069.1. [O14681-1]
UniGeneiHs.643514.

Genome annotation databases

EnsembliENST00000278903; ENSP00000278903; ENSG00000149547.
ENST00000534546; ENSP00000479943; ENSG00000149547. [O14681-3]
ENST00000620753; ENSP00000484510; ENSG00000149547.
GeneIDi9538.
KEGGihsa:9538.
UCSCiuc001qca.3. human. [O14681-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF010313 mRNA. Translation: AAC39531.2. Different initiation.
AK291951 mRNA. Translation: BAF84640.1.
AK296620 mRNA. Translation: BAG59228.1.
AK315841 mRNA. Translation: BAF98732.1.
CH471065 Genomic DNA. Translation: EAW67641.1.
BC002390 mRNA. Translation: AAH02390.1.
CCDSiCCDS73410.1. [O14681-1]
CCDS76493.1. [O14681-3]
RefSeqiNP_001277064.1. NM_001290135.1. [O14681-3]
NP_004870.3. NM_004879.4. [O14681-1]
XP_011541371.1. XM_011543069.1. [O14681-1]
UniGeneiHs.643514.

3D structure databases

ProteinModelPortaliO14681.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO14681. 2 interactions.
STRINGi9606.ENSP00000278903.

Protein family/group databases

TCDBi2.A.121.3.1. the sulfate transporter (cysz) family.

PTM databases

PhosphoSiteiO14681.

Polymorphism and mutation databases

BioMutaiEI24.

Proteomic databases

MaxQBiO14681.
PaxDbiO14681.
PRIDEiO14681.

Protocols and materials databases

DNASUi9538.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000278903; ENSP00000278903; ENSG00000149547.
ENST00000534546; ENSP00000479943; ENSG00000149547. [O14681-3]
ENST00000620753; ENSP00000484510; ENSG00000149547.
GeneIDi9538.
KEGGihsa:9538.
UCSCiuc001qca.3. human. [O14681-1]

Organism-specific databases

CTDi9538.
GeneCardsiGC11P125441.
H-InvDBHIX0010241.
HGNCiHGNC:13276. EI24.
HPAiHPA047165.
HPA051029.
MIMi605170. gene.
neXtProtiNX_O14681.
PharmGKBiPA134937367.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG266932.
GeneTreeiENSGT00390000018633.
HOGENOMiHOG000293197.
HOVERGENiHBG001857.
InParanoidiO14681.
KOiK10134.
OMAiLFHKTVH.
PhylomeDBiO14681.
TreeFamiTF314441.

Miscellaneous databases

ChiTaRSiEI24. human.
GeneWikiiEI24.
GenomeRNAii9538.
NextBioi35472816.
PROiO14681.
SOURCEiSearch...

Gene expression databases

BgeeiO14681.
CleanExiHS_EI24.
ExpressionAtlasiO14681. baseline and differential.
GenevisibleiO14681. HS.

Family and domain databases

InterProiIPR009890. EI24.
[Graphical view]
PANTHERiPTHR21389. PTHR21389. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INDUCTION BY TP53.
    Tissue: Colon cancer.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Colon and Synovium.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lung.
  5. "Candidate tumour suppressor genes at 11q23-q24 in breast cancer: evidence of alterations in PIG8, a gene involved in p53-induced apoptosis."
    Gentile M., Ahnstrom M., Schon F., Wingren S.
    Oncogene 20:7753-7760(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE INVOLVEMENT IN BREAST CANCER, VARIANTS GLY-30; TRP-195; ASP-196; TYR-197; HIS-199 AND ALA-319.
  6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326 AND SER-330, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Inactivation of CHEK1 and EI24 are associated with the development of invasive cervical carcinoma: Clinical and prognostic implications."
    Mazumder ' Indra ' D., Mitra S., Singh R.K., Dutta S., Roy A., Mondal R.K., Basu P.S., Roychoudhury S., Panda C.K.
    Int. J. Cancer 129:1859-1871(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, POSSIBLE INVOLVEMENT IN CERVICAL CANCER.
  13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  14. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-330, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiEI24_HUMAN
AccessioniPrimary (citable) accession number: O14681
Secondary accession number(s): A8K7D6, B4DKL6, Q9BUQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 5, 2009
Last modified: July 22, 2015
This is version 119 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.