O14672 (ADA10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Disintegrin and metalloproteinase domain-containing protein 10
Short name=ADAM 10
EC=3.4.24.81

Alternative name(s):
CDw156
Kuzbanian protein homolog
Mammalian disintegrin-metalloprotease
CD_antigen=CD156c

Gene names

Name:	ADAM10
Synonyms:	KUZ, MADM

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	748 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling. Ref.4 Ref.5 Ref.7 Ref.8 Ref.10 Ref.11 Ref.14 Ref.16
Catalytic activity	Endopeptidase of broad specificity.
Cofactor	Binds 1 zinc ion By similarity.
Subunit structure	Interacts with EPHA2 By similarity. Forms a ternary EFNA5-EPHA3-ADAM10 complex mediating EFNA5 extracellular domain shedding by ADAM10 which regulates the EFNA5-EPHA3 complex internalization and function, the cleavage occurs in trans, with ADAM10 and its substrate being on the membranes of opposing cells. Ref.8
Subcellular location	Cell membrane; Single-pass type I membrane protein. Endomembrane system; Single-pass type I membrane protein. Note: Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
Tissue specificity	Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver. Ref.3 Ref.6
Induction	In osteoarthritis affected-cartilage.
Domain	The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. The Cys-rich region C-terminal to the disintegrin domain functions as a substrate-recognition module, it recognizes the EFNA5-EPHA3 Complex but not the individual proteins By similarity.
Post-translational modification	The precursor is cleaved by a furin endopeptidase By similarity.
Sequence similarities	Contains 1 disintegrin domain. Contains 1 peptidase M12B domain.

Ontologies

Keywords
Biological process	Notch signaling pathway
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	SH3-binding Signal Transmembrane Transmembrane helix
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Zymogen
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	Notch receptor processing Traceable author statement. Source: Reactome Notch signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB PMA-inducible membrane protein ectodomain proteolysis Inferred from mutant phenotype PubMed 18676862. Source: BHF-UCL cell-cell signaling Non-traceable author statement PubMed 12714508. Source: UniProtKB constitutive protein ectodomain proteolysis Inferred from direct assay PubMed 12714508. Source: UniProtKB epidermal growth factor receptor signaling pathway Traceable author statement. Source: Reactome in utero embryonic development Inferred from sequence or structural similarity. Source: UniProtKB integrin-mediated signaling pathway Non-traceable author statement Ref.2. Source: UniProtKB monocyte activation Inferred from mutant phenotype PubMed 11831872. Source: BHF-UCL negative regulation of cell adhesion Inferred from direct assay PubMed 12714508. Source: UniProtKB positive regulation of T cell chemotaxis Inferred from mutant phenotype PubMed 18373975. Source: BHF-UCL positive regulation of cell growth Inferred from mutant phenotype PubMed 18373975. Source: BHF-UCL positive regulation of cell proliferation Inferred from mutant phenotype PubMed 18373975. Source: BHF-UCL protein phosphorylation Inferred from sequence or structural similarity. Source: UniProtKB response to tumor necrosis factor Inferred from direct assay PubMed 11831872. Source: BHF-UCL
Cellular_component	Golgi-associated vesicle Inferred from direct assay Ref.4. Source: UniProtKB cell surface Inferred from direct assay Ref.4. Source: UniProtKB endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Non-traceable author statement Ref.2. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB plasma membrane Traceable author statement. Source: Reactome postsynaptic density Inferred from electronic annotation. Source: Compara
Molecular_function	integrin binding Non-traceable author statement Ref.2. Source: UniProtKB metalloendopeptidase activity Inferred from mutant phenotype Ref.11. Source: UniProtKB protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB protein kinase binding Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 213	194	By similarity		PRO_0000029066
	Chain	214 – 748	535	Disintegrin and metalloproteinase domain-containing protein 10		PRO_0000029067
Regions
	Topological domain	20 – 672	653	Extracellular Potential
	Transmembrane	673 – 693	21	Helical; Potential
	Topological domain	694 – 748	55	Cytoplasmic Potential
	Domain	220 – 456	237	Peptidase M12B
	Domain	457 – 551	95	Disintegrin
	Motif	171 – 178	8	Cysteine switch By similarity
	Motif	708 – 715	8	SH3-binding Potential
	Motif	722 – 728	7	SH3-binding Potential
	Compositional bias	555 – 673	119	Cys-rich
Sites
	Active site	384	1
	Metal binding	173	1	Zinc; in inhibited form By similarity
	Metal binding	383	1	Zinc; catalytic
	Metal binding	387	1	Zinc; catalytic
	Metal binding	393	1	Zinc; catalytic
Amino acid modifications
	Glycosylation	267	1	N-linked (GlcNAc...) Potential
	Glycosylation	278	1	N-linked (GlcNAc...) Ref.9 Ref.12 Ref.13
	Glycosylation	439	1	N-linked (GlcNAc...) Potential
	Glycosylation	551	1	N-linked (GlcNAc...) Potential
	Disulfide bond	222 ↔ 313		By similarity
	Disulfide bond	344 ↔ 451		By similarity
	Disulfide bond	399 ↔ 435		By similarity
	Disulfide bond	484 ↔ 515		By similarity
	Disulfide bond	503 ↔ 511		By similarity
	Disulfide bond	524 ↔ 543		By similarity
	Disulfide bond	530 ↔ 562		By similarity
	Disulfide bond	555 ↔ 567		By similarity
	Disulfide bond	572 ↔ 598		By similarity
	Disulfide bond	580 ↔ 607		By similarity
	Disulfide bond	582 ↔ 597		By similarity
	Disulfide bond	594 ↔ 639		By similarity
	Disulfide bond	632 ↔ 645		By similarity
Natural variations
	Natural variant	176	1	H → Y in a cutaneous metastatic melanoma sample; somatic mutation. Ref.17		VAR_066309
Experimental info
	Sequence conflict	162	1	N → SERLKLRLRKLMSLELWTSC CLPCALLLHSWKKAVNSHCL YFKDFWGFSEIY in CAA88463. Ref.2
	Sequence conflict	212	1	K → R in CAA88463. Ref.2
	Sequence conflict	296	1	G → S in CAA88463. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O14672 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 0881E65B17022A71	FASTA	748	84,142
	10 20 30 40 50 60 MVLLRVLILL LSWAAGMGGQ YGNPLNKYIR HYEGLSYNVD SLHQKHQRAK RAVSHEDQFL 70 80 90 100 110 120 RLDFHAHGRH FNLRMKRDTS LFSDEFKVET SNKVLDYDTS HIYTGHIYGE EGSFSHGSVI 130 140 150 160 170 180 DGRFEGFIQT RGGTFYVEPA ERYIKDRTLP FHSVIYHEDD INYPHKYGPQ GGCADHSVFE 190 200 210 220 230 240 RMRKYQMTGV EEVTQIPQEE HAANGPELLR KKRTTSAEKN TCQLYIQTDH LFFKYYGTRE 250 260 270 280 290 300 AVIAQISSHV KAIDTIYQTT DFSGIRNISF MVKRIRINTT ADEKDPTNPF RFPNIGVEKF 310 320 330 340 350 360 LELNSEQNHD DYCLAYVFTD RDFDDGVLGL AWVGAPSGSS GGICEKSKLY SDGKKKSLNT 370 380 390 400 410 420 GIITVQNYGS HVPPKVSHIT FAHEVGHNFG SPHDSGTECT PGESKNLGQK ENGNYIMYAR 430 440 450 460 470 480 ATSGDKLNNN KFSLCSIRNI SQVLEKKRNN CFVESGQPIC GNGMVEQGEE CDCGYSDQCK 490 500 510 520 530 540 DECCFDANQP EGRKCKLKPG KQCSPSQGPC CTAQCAFKSK SEKCRDDSDC AREGICNGFT 550 560 570 580 590 600 ALCPASDPKP NFTDCNRHTQ VCINGQCAGS ICEKYGLEEC TCASSDGKDD KELCHVCCMK 610 620 630 640 650 660 KMDPSTCAST GSVQWSRHFS GRTITLQPGS PCNDFRGYCD VFMRCRLVDA DGPLARLKKA 670 680 690 700 710 720 IFSPELYENI AEWIVAHWWA VLLMGIALIM LMAGFIKICS VHTPSSNPKL PPPKPLPGTL 730 740 KRRRPPQPIQ QPQRQRPRES YQMGHMRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a pro-tumor necrosis factor-alpha-processing enzyme from the ADAM family of zinc metalloproteases." Rosendahl M.S., Ko S.C., Long D.L., Brewer M.T., Rosenzweig B., Hedl E., Anderson L., Pyle S.M., Moreland J., Meyers M.A., Kohno T., Lyons D., Lichenstein H.S. J. Biol. Chem. 272:24588-24593(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 216-237.
[2]	"Molecular cloning of MADM: a catalytically active mammalian disintegrin-metalloprotease expressed in various cell types." Howard L., Mitchell S., Lu X., Griffiths S., Glynn P. Biochem. J. 317:45-50(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 109-748.
[3]	"Expression of members of a novel membrane linked metalloproteinase family (ADAM) in human articular chondrocytes." McKie N., Edwards T., Dallas D.J., Houghton A., Stringer B., Graham R., Russell G., Croucher P.I. Biochem. Biophys. Res. Commun. 230:335-339(1997) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[4]	"ADAM10-mediated cleavage of L1 adhesion molecule at the cell surface and in released membrane vesicles." Gutwein P., Mechtersheimer S., Riedle S., Stoeck A., Gast D., Joumaa S., Zentgraf H., Fogel M., Altevogt P. FASEB J. 17:292-294(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"The disintegrins ADAM10 and TACE contribute to the constitutive and phorbol ester-regulated normal cleavage of the cellular prion protein." Vincent B., Paitel E., Saftig P., Frobert Y., Hartmann D., De Strooper B., Grassi J., Lopez-Perez E., Checler F. J. Biol. Chem. 276:37743-37746(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"ADAM-10 protein is present in human articular cartilage primarily in the membrane-bound form and is upregulated in osteoarthritis and in response to IL-1alpha in bovine nasal cartilage." Chubinskaya S., Mikhail R., Deutsch A., Tindal M.H. J. Histochem. Cytochem. 49:1165-1176(2001) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"Platelet-activating factor receptor and ADAM10 mediate responses to Staphylococcus aureus in epithelial cells." Lemjabbar H., Basbaum C. Nat. Med. 8:41-46(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Adam meets Eph: an ADAM substrate recognition module acts as a molecular switch for ephrin cleavage in trans." Janes P.W., Saha N., Barton W.A., Kolev M.V., Wimmer-Kleikamp S.H., Nievergall E., Blobel C.P., Himanen J.P., Lackmann M., Nikolov D.B. Cell 123:291-304(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH EFNA5 AND EPHA3, FUNCTION IN EFNA5-EPHA3 SIGNALING.
[9]	"Elucidation of N-glycosylation sites on human platelet proteins: a glycoproteomic approach." Lewandrowski U., Moebius J., Walter U., Sickmann A. Mol. Cell. Proteomics 5:226-233(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-278, MASS SPECTROMETRY. Tissue: Platelet.
[10]	"The Fas ligand intracellular domain is released by ADAM10 and SPPL2a cleavage in T-cells." Kirkin V., Cahuzac N., Guardiola-Serrano F., Huault S., Luckerath K., Friedmann E., Novac N., Wels W.S., Martoglio B., Hueber A.O., Zornig M. Cell Death Differ. 14:1678-1687(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN CLEAVAGE OF FASLG.
[11]	"Substrate requirements for SPPL2b-dependent regulated intramembrane proteolysis." Martin L., Fluhrer R., Haass C. J. Biol. Chem. 284:5662-5670(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN CLEAVAGE OF ITM2B.
[12]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-278, MASS SPECTROMETRY. Tissue: Liver.
[13]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-278, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[14]	"Junctional adhesion molecule-C is a soluble mediator of angiogenesis." Rabquer B.J., Amin M.A., Teegala N., Shaheen M.K., Tsou P.S., Ruth J.H., Lesch C.A., Imhof B.A., Koch A.E. J. Immunol. 185:1777-1785(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN CLEAVAGE OF JAM3.
[15]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]	"Ectodomain shedding and autocleavage of the cardiac membrane protease corin." Jiang J., Wu S., Wang W., Chen S., Peng J., Zhang X., Wu Q. J. Biol. Chem. 286:10066-10072(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN CLEAVAGE OF CORIN.
[17]	"Analysis of the disintegrin-metalloproteinases family reveals ADAM29 and ADAM7 are often mutated in melanoma." Wei X., Moncada-Pazos A., Cal S., Soria-Valles C., Gartner J., Rudloff U., Lin J.C., Rosenberg S.A., Lopez-Otin C., Samuels Y. Hum. Mutat. 32:E2148-E2175(2011) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT TYR-176.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF009615 mRNA. Translation: AAC51766.1.
Z48579 mRNA. Translation: CAA88463.1.

IPI

IPI00013897.

RefSeq

NP_001101.1. NM_001110.2.

UniGene

Hs.578508.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1M1I	model	-	A	207-453	[»]

ProteinModelPortal

O14672.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-39889N.

IntAct

O14672. 3 interactions.

MINT

MINT-5000775.

STRING

9606.ENSP00000260408.

Protein family/group databases

MEROPS

M12.210.

PTM databases

PhosphoSite

O14672.

Proteomic databases

PaxDb

O14672.

PRIDE

O14672.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000260408; ENSP00000260408; ENSG00000137845.

GeneID

102.

KEGG

hsa:102.

UCSC

uc002afd.1. human.

Organism-specific databases

CTD

102.

GeneCards

GC15M058887.

HGNC

HGNC:188. ADAM10.

HPA

CAB001709.

MIM

602192. gene.

neXtProt

NX_O14672.

PharmGKB

PA24505.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG271989.

HOGENOM

HOG000008148.

HOVERGEN

HBG050455.

InParanoid

O14672.

KO

K06704.

OMA

HEDDIKY.

OrthoDB

EOG457562.

PhylomeDB

O14672.

Enzyme and pathway databases

BioCyc

MetaCyc:ENSG00000137845-MONOMER.

BRENDA

3.4.24.81. 2681.

Pathway_Interaction_DB

ps1pathway. Presenilin action in Notch and Wnt signaling.

Reactome

REACT_111102. Signal Transduction.
REACT_116125. Disease.
REACT_118779. Extracellular matrix organization.
REACT_2001. Receptor-ligand binding initiates the second proteolytic cleavage of Notch receptor.

Gene expression databases

ArrayExpress

O14672.

Bgee

O14672.

CleanEx

HS_ADAM10.

Genevestigator

O14672.

GermOnline

ENSG00000137845. Homo sapiens.

Family and domain databases

Gene3D

3.40.390.10. 1 hit.
4.10.70.10. 1 hit.

InterPro

IPR027053. ADAM_10.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]

PANTHER

PTHR11905:SF4. PTHR11905:SF4. 1 hit.

Pfam

PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
[Graphical view]

SMART

SM00050. DISIN. 1 hit.
[Graphical view]

SUPFAM

SSF57552. Disintegrin. 1 hit.

PROSITE

PS50215. ADAM_MEPRO. 1 hit.
PS00546. CYSTEINE_SWITCH. False negative.
PS00427. DISINTEGRIN_1. False negative.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

O14672.

ChEMBL

CHEMBL5028.

ChiTaRS

ADAM10. human.

GenomeRNAi

102.

NextBio

385.

PMAP-CutDB

O14672.

SOURCE

Search...

Entry information

Entry name

ADA10_HUMAN

Accession

Primary (citable) accession number: O14672
Secondary accession number(s): Q10742, Q92650

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 28, 2003
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 133 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.