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Protein

Golgi SNAP receptor complex member 2

Gene

GOSR2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network.

GO - Molecular functioni

  1. SNAP receptor activity Source: GO_Central
  2. SNARE binding Source: GO_Central
  3. transporter activity Source: ProtInc

GO - Biological processi

  1. activation of signaling protein activity involved in unfolded protein response Source: Reactome
  2. cellular protein metabolic process Source: Reactome
  3. endoplasmic reticulum unfolded protein response Source: Reactome
  4. ER to Golgi vesicle-mediated transport Source: GO_Central
  5. Golgi to vacuole transport Source: GO_Central
  6. intra-Golgi vesicle-mediated transport Source: GO_Central
  7. membrane fusion Source: ProtInc
  8. protein targeting to vacuole Source: GO_Central
  9. retrograde transport, endosome to Golgi Source: GO_Central
  10. vesicle fusion with Golgi apparatus Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_18273. XBP1(S) activates chaperone genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi SNAP receptor complex member 2
Alternative name(s):
27 kDa Golgi SNARE protein
Membrin
Gene namesi
Name:GOSR2
Synonyms:GS27
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:4431. GOSR2.

Subcellular locationi

Golgi apparatuscis-Golgi network membrane By similarity; Single-pass type IV membrane protein By similarity. Golgi apparatus membrane By similarity
Note: Concentrated most in the intermediate compartment/cis-Golgi network and the cis-Golgi cisternae 1 and 2. Greatly reduced in concentration at the trans end of the Golgi apparatus (By similarity).By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 190190CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei191 – 21121Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
BLAST
Topological domaini212 – 2121VesicularSequence Analysis

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: GO_Central
  2. ER to Golgi transport vesicle membrane Source: GO_Central
  3. Golgi apparatus Source: UniProtKB
  4. Golgi membrane Source: Reactome
  5. Golgi stack Source: InterPro
  6. integral component of membrane Source: UniProtKB-KW
  7. late endosome membrane Source: GO_Central
  8. membrane Source: UniProtKB
  9. SNARE complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Involvement in diseasei

Epilepsy, progressive myoclonic 61 Publication

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionA neurologic disorder characterized by onset of ataxia in the first years of life, followed by action myoclonus and seizures later in childhood, and loss of independent ambulation in the second decade. Cognition is not usually affected, although mild memory difficulties may occur in the third decade.

See also OMIM:614018
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti144 – 1441G → W in EPM6; loss of function. 1 Publication
VAR_065833

Keywords - Diseasei

Disease mutation, Epilepsy

Organism-specific databases

MIMi614018. phenotype.
Orphaneti280620. Progressive myoclonic epilepsy type 6.
PharmGKBiPA28816.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 212212Golgi SNAP receptor complex member 2PRO_0000212549Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO14653.
PaxDbiO14653.
PRIDEiO14653.

PTM databases

PhosphoSiteiO14653.

Expressioni

Gene expression databases

BgeeiO14653.
CleanExiHS_GOSR2.
ExpressionAtlasiO14653. baseline and differential.
GenevestigatoriO14653.

Organism-specific databases

HPAiCAB017456.
HPA054472.

Interactioni

Subunit structurei

Identified in a unique SNARE complex composed of the Golgi SNAREs GOSR1, STX5 and YKT6.By similarity

Protein-protein interaction databases

BioGridi114940. 12 interactions.
STRINGi9606.ENSP00000225567.

Structurei

Secondary structure

1
212
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi117 – 1193Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3EG9X-ray3.00C116-121[»]
ProteinModelPortaliO14653.
SMRiO14653. Positions 124-180.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO14653.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili61 – 10747Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the GOSR2 family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG148687.
GeneTreeiENSGT00490000043403.
HOGENOMiHOG000231153.
HOVERGENiHBG051765.
InParanoidiO14653.
KOiK08496.
PhylomeDBiO14653.
TreeFamiTF313702.

Family and domain databases

InterProiIPR027027. GOSR2/Membrin/Bos1.
IPR010989. t-SNARE.
[Graphical view]
PIRSFiPIRSF028865. Membrin-2. 1 hit.
SUPFAMiSSF47661. SSF47661. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform A (identifier: O14653-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPLFQQTHK QVHEIQSCMG RLETADKQSV HIVENEIQAS IDQIFSRLER
60 70 80 90 100
LEILSSKEPP NKRQNARLRV DQLKYDVQHL QTALRNFQHR RHAREQQERQ
110 120 130 140 150
REELLSRTFT TNDSDTTIPM DESLQFNSSL QKVHNGMDDL ILDGHNILDG
160 170 180 190 200
LRTQRLTLKG TQKKILDIAN MLGLSNTVMR LIEKRAFQDK YFMIGGMLLT
210
CVVMFLVVQY LT
Length:212
Mass (Da):24,775
Last modified:August 30, 2002 - v2
Checksum:i4D5585CF858A610F
GO
Isoform B (identifier: O14653-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     196-212: GMLLTCVVMFLVVQYLT → TQGSCQTAHFGGRSAGSS

Show »
Length:213
Mass (Da):24,584
Checksum:i990393DAD02BC374
GO
Isoform 3 (identifier: O14653-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     160-212: GTQKKILDIA...VMFLVVQYLT → VGSLLGDREK...LITCPQIVIF

Show »
Length:195
Mass (Da):22,725
Checksum:iAEC4DA42C06B1D2E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti106 – 1061S → C in AAB82651. (PubMed:9349823)Curated
Sequence conflicti113 – 1131D → G in AAB82651. (PubMed:9349823)Curated
Sequence conflicti166 – 1661L → P in AAB82651. (PubMed:9349823)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti67 – 671R → K.
Corresponds to variant rs197922 [ dbSNP | Ensembl ].
VAR_024471
Natural varianti144 – 1441G → W in EPM6; loss of function. 1 Publication
VAR_065833

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei160 – 21253GTQKK…VQYLT → VGSLLGDREKASCFSLIQQF SNCVYILITCPQIVIF in isoform 3. 2 PublicationsVSP_043200Add
BLAST
Alternative sequencei196 – 21217GMLLT…VQYLT → TQGSCQTAHFGGRSAGSS in isoform B. 1 PublicationVSP_001829Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007548 mRNA. Translation: AAB82651.1.
AF229796 mRNA. Translation: AAK01855.1.
AK290890 mRNA. Translation: BAF83579.1.
AC005670 Genomic DNA. No translation available.
CH471231 Genomic DNA. Translation: EAW57694.1.
CH471231 Genomic DNA. Translation: EAW57695.1.
CH471231 Genomic DNA. Translation: EAW57699.1.
CH471231 Genomic DNA. Translation: EAW57700.1.
BC034762 mRNA. Translation: AAH34762.1.
BC009710 mRNA. Translation: AAH09710.1.
CCDSiCCDS11507.1. [O14653-2]
CCDS42355.1. [O14653-1]
CCDS45719.1. [O14653-3]
RefSeqiNP_001012529.1. NM_001012511.1. [O14653-3]
NP_004278.2. NM_004287.3. [O14653-1]
NP_473363.1. NM_054022.2. [O14653-2]
UniGeneiHs.463278.

Genome annotation databases

EnsembliENST00000225567; ENSP00000225567; ENSG00000108433. [O14653-2]
ENST00000393456; ENSP00000377101; ENSG00000108433. [O14653-1]
ENST00000415811; ENSP00000394559; ENSG00000108433. [O14653-3]
GeneIDi9570.
KEGGihsa:9570.
UCSCiuc002iky.3. human. [O14653-3]
uc002ikz.3. human. [O14653-2]
uc002ila.3. human. [O14653-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007548 mRNA. Translation: AAB82651.1.
AF229796 mRNA. Translation: AAK01855.1.
AK290890 mRNA. Translation: BAF83579.1.
AC005670 Genomic DNA. No translation available.
CH471231 Genomic DNA. Translation: EAW57694.1.
CH471231 Genomic DNA. Translation: EAW57695.1.
CH471231 Genomic DNA. Translation: EAW57699.1.
CH471231 Genomic DNA. Translation: EAW57700.1.
BC034762 mRNA. Translation: AAH34762.1.
BC009710 mRNA. Translation: AAH09710.1.
CCDSiCCDS11507.1. [O14653-2]
CCDS42355.1. [O14653-1]
CCDS45719.1. [O14653-3]
RefSeqiNP_001012529.1. NM_001012511.1. [O14653-3]
NP_004278.2. NM_004287.3. [O14653-1]
NP_473363.1. NM_054022.2. [O14653-2]
UniGeneiHs.463278.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3EG9X-ray3.00C116-121[»]
ProteinModelPortaliO14653.
SMRiO14653. Positions 124-180.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114940. 12 interactions.
STRINGi9606.ENSP00000225567.

PTM databases

PhosphoSiteiO14653.

Proteomic databases

MaxQBiO14653.
PaxDbiO14653.
PRIDEiO14653.

Protocols and materials databases

DNASUi9570.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000225567; ENSP00000225567; ENSG00000108433. [O14653-2]
ENST00000393456; ENSP00000377101; ENSG00000108433. [O14653-1]
ENST00000415811; ENSP00000394559; ENSG00000108433. [O14653-3]
GeneIDi9570.
KEGGihsa:9570.
UCSCiuc002iky.3. human. [O14653-3]
uc002ikz.3. human. [O14653-2]
uc002ila.3. human. [O14653-1]

Organism-specific databases

CTDi9570.
GeneCardsiGC17P045000.
HGNCiHGNC:4431. GOSR2.
HPAiCAB017456.
HPA054472.
MIMi604027. gene.
614018. phenotype.
neXtProtiNX_O14653.
Orphaneti280620. Progressive myoclonic epilepsy type 6.
PharmGKBiPA28816.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG148687.
GeneTreeiENSGT00490000043403.
HOGENOMiHOG000231153.
HOVERGENiHBG051765.
InParanoidiO14653.
KOiK08496.
PhylomeDBiO14653.
TreeFamiTF313702.

Enzyme and pathway databases

ReactomeiREACT_18273. XBP1(S) activates chaperone genes.

Miscellaneous databases

ChiTaRSiGOSR2. human.
EvolutionaryTraceiO14653.
GeneWikiiGOSR2.
GenomeRNAii9570.
NextBioi35891.
PROiO14653.
SOURCEiSearch...

Gene expression databases

BgeeiO14653.
CleanExiHS_GOSR2.
ExpressionAtlasiO14653. baseline and differential.
GenevestigatoriO14653.

Family and domain databases

InterProiIPR027027. GOSR2/Membrin/Bos1.
IPR010989. t-SNARE.
[Graphical view]
PIRSFiPIRSF028865. Membrin-2. 1 hit.
SUPFAMiSSF47661. SSF47661. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A SNARE involved in protein transport through the Golgi apparatus."
    Lowe S.L., Peter F., Subramaniam V.N., Wong S.H., Hong W.
    Nature 389:881-884(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
  2. "cDNA characterization and chromosomal mapping of human Golgi SNARE GS27 and GS28 to chromosome 17."
    Bui T.D., Levy E.R., Subramaniam V.N., Lowe S.L., Hong W.
    Genomics 57:285-288(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
  3. "Gene organization of human Golgi SNARE GS27."
    Bui T.D., Hong W.
    Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
  5. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND 3).
    Tissue: Colon and Lung.
  8. Bienvenut W.V., Lao L., Ryan K.M.
    Submitted (JUN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 1-10; 51-63 AND 75-85, ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Osteosarcoma.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: VARIANT EPM6 TRP-144, CHARACTERIZATION OF VARIANT EPM6 TRP-144.

Entry informationi

Entry nameiGOSR2_HUMAN
AccessioniPrimary (citable) accession number: O14653
Secondary accession number(s): D3DXJ5
, D3DXJ6, Q8N4B8, Q96DA5, Q9BZZ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 30, 2002
Last modified: February 4, 2015
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.