O14649 (KCNK3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium channel subfamily K member 3

Alternative name(s):
Acid-sensitive potassium channel protein TASK-1
TWIK-related acid-sensitive K(+) channel 1
Two pore potassium channel KT3.1
Short name=Two pore K(+) channel KT3.1

Gene names

Name:	KCNK3
Synonyms:	TASK, TASK1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	pH-dependent, voltage-insensitive, background potassium channel protein. Rectification direction results from potassium ion concentration on either side of the membrane. Acts as an outward rectifier when external potassium concentration is low. When external potassium concentration is high, current is inward.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Widespread expression in adult. Strongest expression in pancreas and placenta. Lower expression in brain, lung, prostate, heart, kidney, uterus, small intestine and colon.
Miscellaneous	Inhibited by external acidification. Activated by halothane and isoflurane.
Sequence similarities	Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Potassium channel Voltage-gated channel
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	brain development Inferred from electronic annotation. Source: Compara cellular response to hypoxia Inferred from electronic annotation. Source: Compara cellular response to zinc ion Inferred from electronic annotation. Source: Compara cochlea development Inferred from electronic annotation. Source: Compara reduction of cytosolic calcium ion concentration Inferred from electronic annotation. Source: Compara response to drug Inferred from electronic annotation. Source: Compara synaptic transmission Traceable author statement. Source: Reactome
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	open rectifier potassium channel activity Inferred from electronic annotation. Source: Compara potassium channel activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Potassium channel subfamily K member 3		PRO_0000101744
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 29	21	Helical; Potential
	Intramembrane	78 – 101	24	Pore-forming; Name=Pore-forming 1; Potential
	Transmembrane	108 – 128	21	Helical; Potential
	Topological domain	129 – 158	30	Cytoplasmic Potential
	Transmembrane	159 – 179	21	Helical; Potential
	Intramembrane	184 – 207	24	Pore-forming; Name=Pore-forming 2; Potential
	Transmembrane	223 – 243	21	Helical; Potential
	Topological domain	244 – 394	151	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	53	1	N-linked (GlcNAc...) Potential
Experimental info
	Mutagenesis	98	1	H → N: Greatly reduces pH sensitivity. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	O14649 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 9FF4C8266F615FB7	FASTA	394	43,518
	10 20 30 40 50 60 MKRQNVRTLA LIVCTFTYLL VGAAVFDALE SEPELIERQR LELRQQELRA RYNLSQGGYE 70 80 90 100 110 120 ELERVVLRLK PHKAGVQWRF AGSFYFAITV ITTIGYGHAA PSTDGGKVFC MFYALLGIPL 130 140 150 160 170 180 TLVMFQSLGE RINTLVRYLL HRAKKGLGMR RADVSMANMV LIGFFSCIST LCIGAAAFSH 190 200 210 220 230 240 YEHWTFFQAY YYCFITLTTI GFGDYVALQK DQALQTQPQY VAFSFVYILT GLTVIGAFLN 250 260 270 280 290 300 LVVLRFMTMN AEDEKRDAEH RALLTRNGQA GGGGGGGSAH TTDTASSTAA AGGGGFRNVY 310 320 330 340 350 360 AEVLHFQSMC SCLWYKSREK LQYSIPMIIP RDLSTSDTCV EQSHSSPGGG GRYSDTPSRR 370 380 390 CLCSGAPRSA ISSVSTGLHS LSTFRGLMKR RSSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TASK, a human background K+ channel to sense external pH variations near physiological pH." Duprat F., Lesage F., Fink M., Reyes R., Heurteaux C., Lazdunski M. EMBO J. 16:5464-5471(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[2]	"Proton block and voltage gating are potassium-dependent in the cardiac leak channel Kcnk3." Lopes C.M.B., Gallagher P.G., Buck M.E., Butler M.H., Goldstein S.A.N. J. Biol. Chem. 275:16969-16978(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Inhalational anesthetics activate two-pore-domain background K+ channels." Patel A.J., Honore E., Lesage F., Fink M., Romey G., Lazdunski M. Nat. Neurosci. 2:422-426(1999) [PubMed] [Europe PMC] [Abstract] Cited for: ACTIVATION.
[6]	"TASK-5, a novel member of the tandem pore K+ channel family." Ashmole I., Goodwin P.A., Stanfield P.R. Pflugers Arch. 442:828-833(2001) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF HIS-98.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF006823 mRNA. Translation: AAC51777.1. AF065163 mRNA. Translation: AAG29340.1. AC015977 Genomic DNA. Translation: AAY24312.1. CH471053 Genomic DNA. Translation: EAX00678.1. CH471053 Genomic DNA. Translation: EAX00679.1.
IPI	IPI00023110.
RefSeq	NP_002237.1. NM_002246.2.
UniGene	Hs.645288.
3D structure databases
ProteinModelPortal	O14649.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1645466.
STRING	9606.ENSP00000306275.
Protein family/group databases
TCDB	1.A.1.9.2. voltage-gated ion channel (VIC) superfamily.
PTM databases
PhosphoSite	O14649.
Proteomic databases
PaxDb	O14649.
PRIDE	O14649.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000302909; ENSP00000306275; ENSG00000171303.
GeneID	3777.
KEGG	hsa:3777.
UCSC	uc002rhn.2. human.
Organism-specific databases
CTD	3777.
GeneCards	GC02P026827.
HGNC	HGNC:6278. KCNK3.
HPA	HPA026658.
MIM	603220. gene.
neXtProt	NX_O14649.
PharmGKB	PA30060.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG259548.
HOGENOM	HOG000231463.
HOVERGEN	HBG052239.
InParanoid	O14649.
KO	K04914.
OMA	CSGTQRS.
OrthoDB	EOG4VX25V.
PhylomeDB	O14649.
Enzyme and pathway databases
Reactome	REACT_13685. Neuronal System.
Gene expression databases
ArrayExpress	O14649.
Bgee	O14649.
CleanEx	HS_KCNK3.
Genevestigator	O14649.
GermOnline	ENSG00000171303. Homo sapiens.
Family and domain databases
InterPro	IPR003280. 2pore_dom_K_chnl. IPR003092. 2pore_dom_K_chnl_TASK. IPR022306. 2pore_dom_K_chnl_TASK/TWIK. IPR005406. 2pore_dom_K_chnl_TASK1. IPR013099. Ion_trans_2. [Graphical view]
PANTHER	PTHR11003:SF16. PTHR11003:SF16. 1 hit.
Pfam	PF07885. Ion_trans_2. 2 hits. [Graphical view]
PIRSF	PIRSF038061. K_channel_subfamily_K_type. 1 hit.
PRINTS	PR01333. 2POREKCHANEL. PR01584. TASK1CHANNEL. PR01095. TASKCHANNEL.
ProtoNet	Search...
Other
GenomeRNAi	3777.
NextBio	14819.
SOURCE	Search...

Entry information

Entry name

KCNK3_HUMAN

Accession

Primary (citable) accession number: O14649
Secondary accession number(s): Q53SU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.