O14625 (CXL11_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-X-C motif chemokine 11

Alternative name(s):
Beta-R1
H174
Interferon gamma-inducible protein 9
Short name=IP-9
Interferon-inducible T-cell alpha chemoattractant
Short name=I-TAC
Small-inducible cytokine B11

Gene names

Name:	CXCL11
Synonyms:	ITAC, SCYB11, SCYB9B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	94 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
Subcellular location	Secreted.
Tissue specificity	High levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders.
Induction	By IFNG/IFN-gamma and IFNB1/IFN-beta. Induction by IFNG/IFN-gamma is enhanced by TNF in monocytes, dermal fibroblasts and endothelial cells, and by IL1/interleukin-1 in astrocytes.
Sequence similarities	Belongs to the intercrine alpha (chemokine CxC) family.
Mass spectrometry	Molecular mass is 8303 Da from positions 22 - 94. Determined by MALDI. Ref.4
Sequence caution	The sequence AAB17374.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Chemotaxis Inflammatory response
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Cytokine
PTM	Citrullination Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell-cell signaling Traceable author statement Ref.4. Source: ProtInc chemotaxis Traceable author statement Ref.4. Source: ProtInc immune response Inferred from electronic annotation. Source: Compara inflammatory response Traceable author statement Ref.4. Source: ProtInc signal transduction Traceable author statement Ref.4. Source: ProtInc
Cellular_component	extracellular region Traceable author statement. Source: Reactome extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	chemokine activity Traceable author statement Ref.4. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

21

Ref.4

Chain

22 – 94

73

C-X-C motif chemokine 11

PRO_0000005106

Amino acid modifications

Modified residue

27

1

Citrulline; by PAD2

Disulfide bond

30 ↔ 57

Ref.11

Disulfide bond

32 ↔ 74

Ref.11

Natural variations

Natural variant

55

1

N → S.
Corresponds to variant rs4859596 [ dbSNP | Ensembl ].

VAR_048700

Experimental info

Sequence conflict

69 – 72

4

NKGQ → IRK in AAB17374. Ref.1

Sequence conflict

78

1

K → NRA in AAB17374. Ref.1

Sequence conflict

89

1

V → A in AAB17374. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

94

Helix Strand Turn

Details...

Turn

54 – 56

3

Beta strand

57 – 59

3

Beta strand

67 – 69

3

Helix

82 – 89

8

Sequences

	Sequence		Length	Mass (Da)
	O14625 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 9D9D8585749EA4CE	FASTA	94	10,365
	10 20 30 40 50 60 MSVKGMAIAL AVILCATVVQ GFPMFKRGRC LCIGPGVKAV KVADIEKASI MYPSNNCDKI 70 80 90 EVIITLKENK GQRCLNPKSK QARLIIKKVE RKNF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of beta-R1, a gene that is selectively induced by interferon beta (IFN-beta) compared with IFN-alpha." Rani M.R.S., Foster G.R., Leung S., Leaman D., Stark G.R., Ransohoff R.M. J. Biol. Chem. 271:22878-22884(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Astrocyte.
[2]	"A genetic selection for isolating cDNAs encoding secreted proteins." Jacobs K.A., Collins-Racie L.A., Colbert M., Duckett M., Golden-Fleet M., Kelleher K., Kriz R., LaVallie E.R., Merberg D., Spaulding V., Stover J., Williamson M.J., McCoy J.M. Gene 198:289-296(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Peripheral blood monocyte.
[3]	"Interferon-inducible T cell alpha chemoattractant (I-TAC): a novel non-ELR CXC chemokine with potent activity on activated T cells through selective high affinity binding to CXCR3." Cole K.E., Strick C.A., Paradis T.J., Ogborne K.T., Loetscher M., Gladue R.P., Lin W., Boyd J.G., Moser B., Wood D.E., Sahagan B.G., Neote K. J. Exp. Med. 187:2009-2021(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal astrocyte.
[4]	"Human IP-9: a keratinocyte derived high affinity CXC-chemokine ligand for the IP-10/Mig receptor (CXCR3)." Tensen C.P., Flier J., van der Raaij-Helmer E.M.H., Sampat-Sardjoepersad S., van der Schors R.C., Leurs R., Scheper R.J., Boorsma D.M., Willemze R. J. Invest. Dermatol. 112:716-722(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-38, MASS SPECTROMETRY. Tissue: Keratinocyte.
[5]	"Structure and expression of the human small cytokine B subfamily member 11 (SCYB11/formerly SCYB9B, alias I-TAC) gene cloned from IFN-gamma-treated human monocytes (THP-1)." Laich A.M., Meyer M., Werner E.R., Werner-Felmayer G. J. Interferon Cytokine Res. 19:505-513(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Myeloma.
[6]	"Genomic organization, sequence and transcriptional regulation of the human CXCL 11 gene." Tensen C.P., Flier J., Rampersad S., Sampat-Sardjoepersad S., Scheper R.J., Boorsma D.M., Willemze R. Biochim. Biophys. Acta 1446:167-172(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Keratinocyte.
[7]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow, Brain and Liver.
[9]	"The human gene encoding SCYB9B, a putative novel CXC chemokine, maps to human chromosome 4q21 like the closely related genes for MIG (SCYB9) and INP10 (SCYB10)." Erdel M., Laich A., Utermann G., Werner E.R., Werner-Felmayer G. Cytogenet. Cell Genet. 81:271-272(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 28-84.
[10]	"Citrullination of CXCL10 and CXCL11 by peptidylarginine deiminase: a naturally occurring posttranslational modification of chemokines and new dimension of immunoregulation." Loos T., Mortier A., Gouwy M., Ronsse I., Put W., Lenaerts J.P., Van Damme J., Proost P. Blood 112:2648-2656(2008) [PubMed] [Europe PMC] [Abstract] Cited for: CITRULLINATION AT ARG-27.
[11]	"NMR structure of CXCR3 binding chemokine CXCL11 (ITAC)." Booth V., Clark-Lewis I., Sykes B.D. Protein Sci. 13:2022-2028(2004) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 22-94, DISULFIDE BONDS.
+	Additional computationally mapped references.

Web resources

Wikipedia

CXCL11 entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U59286 mRNA. Translation: AAB17374.1. Different initiation.
AF002985 mRNA. Translation: AAC51845.1.
AF030514 mRNA. Translation: AAC39775.1.
Y15220 mRNA. Translation: CAA75510.1.
U66096 mRNA. Translation: AAD38867.1.
AF077867 Genomic DNA. Translation: AAD38327.1.
Y15221 Genomic DNA. Translation: CAB51859.1.
BT006787 mRNA. Translation: AAP35433.1.
BC005292 mRNA. Translation: AAH05292.1.
BC012532 mRNA. Translation: AAH12532.1.
BC110986 mRNA. Translation: AAI10987.1.
AF053972 Genomic DNA. Translation: AAD10206.1.

IPI

IPI00023056.

RefSeq

NP_005400.1. NM_005409.4.

UniGene

Hs.632592.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RJT	NMR	-	A	22-94	[»]

ProteinModelPortal

O14625.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5890N.

IntAct

O14625. 1 interaction.

MINT

MINT-153105.

STRING

9606.ENSP00000306884.

PTM databases

PhosphoSite

O14625.

Proteomic databases

PaxDb

O14625.

PRIDE

O14625.

Protocols and materials databases

DNASU

6373.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000306621; ENSP00000306884; ENSG00000169248.
ENST00000503860; ENSP00000425819; ENSG00000169248.

GeneID

6373.

KEGG

hsa:6373.

UCSC

uc003hjm.3. human.

Organism-specific databases

CTD

6373.

GeneCards

GC04M076954.

HGNC

HGNC:10638. CXCL11.

HPA

CAB009921.

MIM

604852. gene.

neXtProt

NX_O14625.

PharmGKB

PA35569.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG43433.

HOVERGEN

HBG107789.

InParanoid

O14625.

KO

K12672.

OMA

GGRCLCI.

OrthoDB

EOG40S0H7.

PhylomeDB

O14625.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

ArrayExpress

O14625.

Bgee

O14625.

CleanEx

HS_CXCL11.

Genevestigator

O14625.

GermOnline

ENSG00000169248. Homo sapiens.

Family and domain databases

InterPro

IPR001089. Chemokine_CXC.
IPR018048. Chemokine_CXC_CS.
IPR001811. Chemokine_IL8-like_dom.
IPR027221. CXCL11.
[Graphical view]

PANTHER

PTHR10179. PTHR10179. 1 hit.
PTHR10179:SF28. PTHR10179:SF28. 1 hit.

Pfam

PF00048. IL8. 1 hit.
[Graphical view]

PRINTS

PR00437. SMALLCYTKCXC.

SMART

SM00199. SCY. 1 hit.
[Graphical view]

SUPFAM

SSF54117. Chemokine_IL8. 1 hit.

PROSITE

PS00471. SMALL_CYTOKINES_CXC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O14625.

GenomeRNAi

6373.

NextBio

24760.

PMAP-CutDB

O14625.

SOURCE

Search...

Entry information

Entry name

CXL11_HUMAN

Accession

Primary (citable) accession number: O14625
Secondary accession number(s): Q53YA3, Q92840

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 135 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.