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Protein

Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2

Gene

CTDSP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells. May contribute to the development of sarcomas.2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+Note: Binds 1 Mg2+ ion per monomer.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1074-aspartylphosphate intermediateBy similarity1
Metal bindingi107Magnesium1 Publication1
Active sitei109Proton donorBy similarity1
Metal bindingi109Magnesium; via carbonyl oxygen1 Publication1
Sitei163Transition state stabilizerBy similarity1
Sitei201Transition state stabilizerBy similarity1
Metal bindingi218Magnesium1 Publication1

GO - Molecular functioni

  • CTD phosphatase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandMetal-binding

Enzyme and pathway databases

ReactomeiR-HSA-381038. XBP1(S) activates chaperone genes.
SignaLinkiO14595.
SIGNORiO14595.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2 (EC:3.1.3.16)
Alternative name(s):
Nuclear LIM interactor-interacting factor 2
Short name:
NLI-interacting factor 2
Protein OS-4
Small C-terminal domain phosphatase 2
Small CTD phosphatase 2
Short name:
SCP2
Gene namesi
Name:CTDSP2
Synonyms:NIF2, OS4, SCP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:17077. CTDSP2.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10106.
OpenTargetsiENSG00000175215.
PharmGKBiPA128394568.

Polymorphism and mutation databases

BioMutaiCTDSP2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002125741 – 271Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2Add BLAST271

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO14595.
MaxQBiO14595.
PaxDbiO14595.
PeptideAtlasiO14595.
PRIDEiO14595.

PTM databases

DEPODiO14595.
iPTMnetiO14595.
PhosphoSitePlusiO14595.
SwissPalmiO14595.

Expressioni

Tissue specificityi

Expression is restricted to non-neuronal tissues. Highest expression in pancreas and lowest in liver.1 Publication

Inductioni

In primary sarcomas.

Gene expression databases

BgeeiENSG00000175215.
CleanExiHS_CTDSP2.
HS_SCP2.
ExpressionAtlasiO14595. baseline and differential.
GenevisibleiO14595. HS.

Organism-specific databases

HPAiHPA052607.

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with REST.By similarity2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi115412. 32 interactors.
DIPiDIP-61245N.
IntActiO14595. 10 interactors.
MINTiMINT-2735588.
STRINGi9606.ENSP00000381148.

Structurei

Secondary structure

1271
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni96 – 100Combined sources5
Beta strandi103 – 106Combined sources4
Turni110 – 112Combined sources3
Beta strandi113 – 118Combined sources6
Beta strandi124 – 131Combined sources8
Beta strandi134 – 142Combined sources9
Helixi146 – 156Combined sources11
Beta strandi157 – 162Combined sources6
Helixi167 – 177Combined sources11
Beta strandi183 – 187Combined sources5
Helixi189 – 191Combined sources3
Beta strandi192 – 195Combined sources4
Beta strandi198 – 200Combined sources3
Helixi203 – 205Combined sources3
Beta strandi206 – 208Combined sources3
Helixi210 – 212Combined sources3
Beta strandi213 – 218Combined sources6
Helixi220 – 223Combined sources4
Helixi227 – 229Combined sources3
Beta strandi230 – 232Combined sources3
Helixi244 – 255Combined sources12
Helixi261 – 268Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Q5EX-ray2.51A/B/C/D/E/F/G/H87-271[»]
ProteinModelPortaliO14595.
SMRiO14595.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO14595.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini97 – 255FCP1 homologyPROSITE-ProRule annotationAdd BLAST159

Phylogenomic databases

eggNOGiKOG1605. Eukaryota.
COG5190. LUCA.
GeneTreeiENSGT00390000017194.
HOGENOMiHOG000236379.
HOVERGENiHBG053298.
InParanoidiO14595.
KOiK15731.
OMAiCNIFKAL.
PhylomeDBiO14595.
TreeFamiTF313556.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiView protein in InterPro
IPR011948. Dullard_phosphatase.
IPR004274. FCP1_dom.
IPR023214. HAD-like_dom.
PfamiView protein in Pfam
PF03031. NIF. 1 hit.
SMARTiView protein in SMART
SM00577. CPDc. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02251. HIF-SF_euk. 1 hit.
PROSITEiView protein in PROSITE
PS50969. FCP1. 1 hit.

Sequencei

Sequence statusi: Complete.

O14595-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHGSIITQA RREDALVLTK QGLVSKSSPK KPRGRNIFKA LFCCFRAQHV
60 70 80 90 100
GQSSSSTELA AYKEEANTIA KSDLLQCLQY QFYQIPGTCL LPEVTEEDQG
110 120 130 140 150
RICVVIDLDE TLVHSSFKPI NNADFIVPIE IEGTTHQVYV LKRPYVDEFL
160 170 180 190 200
RRMGELFECV LFTASLAKYA DPVTDLLDRC GVFRARLFRE SCVFHQGCYV
210 220 230 240 250
KDLSRLGRDL RKTLILDNSP ASYIFHPENA VPVQSWFDDM ADTELLNLIP
260 270
IFEELSGAED VYTSLGQLRA P
Length:271
Mass (Da):30,664
Last modified:November 9, 2004 - v2
Checksum:iE4FA4A1657F2B03F
GO

Sequence cautioni

The sequence AAB71816 differs from that shown. Reason: Frameshift at position 267.Curated
The sequence AAP34399 differs from that shown. Reason: Frameshift at position 267.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9Q → H in AAD09331 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000152 mRNA. Translation: AAB71816.1. Frameshift.
AY279531 mRNA. Translation: AAP34399.1. Frameshift.
AF022231 mRNA. Translation: AAD09331.1.
AK291289 mRNA. Translation: BAF83978.1.
CH471054 Genomic DNA. Translation: EAW97083.1.
BC065920 mRNA. Translation: AAH65920.1.
CCDSiCCDS41801.1.
RefSeqiNP_005721.3. NM_005730.3.
UniGeneiHs.524530.

Genome annotation databases

EnsembliENST00000398073; ENSP00000381148; ENSG00000175215.
GeneIDi10106.
KEGGihsa:10106.
UCSCiuc001sqm.4. human.

Similar proteinsi

Entry informationi

Entry nameiCTDS2_HUMAN
AccessioniPrimary (citable) accession number: O14595
Secondary accession number(s): A8K5H4
, Q53ZR2, Q6NZY3, Q9UEX1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 13, 2001
Last sequence update: November 9, 2004
Last modified: August 30, 2017
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references