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Protein

Neurocan core protein

Gene

NCAN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell adhesion
LigandCalcium, Hyaluronic acid, Lectin

Enzyme and pathway databases

ReactomeiR-HSA-1971475 A tetrasaccharide linker sequence is required for GAG synthesis
R-HSA-2022870 Chondroitin sulfate biosynthesis
R-HSA-2022923 Dermatan sulfate biosynthesis
R-HSA-2024101 CS/DS degradation
R-HSA-3000178 ECM proteoglycans
R-HSA-3560783 Defective B4GALT7 causes EDS, progeroid type
R-HSA-3560801 Defective B3GAT3 causes JDSSDHD
R-HSA-3595172 Defective CHST3 causes SEDCJD
R-HSA-3595174 Defective CHST14 causes EDS, musculocontractural type
R-HSA-3595177 Defective CHSY1 causes TPBS
R-HSA-373760 L1CAM interactions
R-HSA-419037 NCAM1 interactions
R-HSA-4420332 Defective B3GALT6 causes EDSP2 and SEMDJL1

Names & Taxonomyi

Protein namesi
Recommended name:
Neurocan core protein
Alternative name(s):
Chondroitin sulfate proteoglycan 3
Gene namesi
Name:NCAN
Synonyms:CSPG3, NEUR
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000130287.13
HGNCiHGNC:2465 NCAN
MIMi600826 gene
neXtProtiNX_O14594

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi1463
OpenTargetsiENSG00000130287
PharmGKBiPA162396986

Chemistry databases

DrugBankiDB02379 Beta-D-Glucose
DB08818 Hyaluronic acid
DB04396 Thiodigalactoside

Polymorphism and mutation databases

BioMutaiNCAN

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000001751623 – 1321Neurocan core proteinAdd BLAST1299

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi59 ↔ 140By similarity
Glycosylationi122N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi182 ↔ 253By similarity
Disulfide bondi206 ↔ 227By similarity
Disulfide bondi280 ↔ 355By similarity
Disulfide bondi304 ↔ 325By similarity
Glycosylationi340N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1012 ↔ 1023By similarity
Disulfide bondi1017 ↔ 1032By similarity
Glycosylationi1026N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1034 ↔ 1043By similarity
Disulfide bondi1050 ↔ 1061By similarity
Disulfide bondi1055 ↔ 1070By similarity
Disulfide bondi1072 ↔ 1081By similarity
Disulfide bondi1088 ↔ 1099By similarity
Disulfide bondi1116 ↔ 1208By similarity
Disulfide bondi1184 ↔ 1200By similarity
Disulfide bondi1215 ↔ 1258By similarity
Glycosylationi1223N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1244 ↔ 1271By similarity

Post-translational modificationi

O-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Proteoglycan

Proteomic databases

PaxDbiO14594
PeptideAtlasiO14594
PRIDEiO14594

PTM databases

iPTMnetiO14594
PhosphoSitePlusiO14594

Expressioni

Tissue specificityi

Brain.

Gene expression databases

BgeeiENSG00000130287
CleanExiHS_NCAN
ExpressionAtlasiO14594 baseline and differential
GenevisibleiO14594 HS

Organism-specific databases

HPAiHPA036814

Interactioni

Protein-protein interaction databases

BioGridi107845, 5 interactors
IntActiO14594, 1 interactor
STRINGi9606.ENSP00000252575

Structurei

3D structure databases

ProteinModelPortaliO14594
SMRiO14594
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 153Ig-like V-typeAdd BLAST116
Domaini160 – 255Link 1PROSITE-ProRule annotationAdd BLAST96
Domaini259 – 357Link 2PROSITE-ProRule annotationAdd BLAST99
Domaini1008 – 1044EGF-like 1PROSITE-ProRule annotationAdd BLAST37
Domaini1046 – 1082EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST37
Domaini1084 – 1213C-type lectinPROSITE-ProRule annotationAdd BLAST130
Domaini1213 – 1273SushiPROSITE-ProRule annotationAdd BLAST61

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni708 – 712O-glycosylated at one site5

Sequence similaritiesi

Keywords - Domaini

EGF-like domain, Immunoglobulin domain, Repeat, Signal, Sushi

Phylogenomic databases

eggNOGiENOG410IJ1R Eukaryota
ENOG410YR39 LUCA
GeneTreeiENSGT00760000119025
HOVERGENiHBG078994
InParanoidiO14594
KOiK06794
OMAiDWEKDEG
OrthoDBiEOG091G0KN3
PhylomeDBiO14594
TreeFamiTF332134

Family and domain databases

CDDicd00033 CCP, 1 hit
Gene3Di2.60.40.10, 1 hit
3.10.100.10, 3 hits
InterProiView protein in InterPro
IPR001304 C-type_lectin-like
IPR016186 C-type_lectin-like/link_sf
IPR018378 C-type_lectin_CS
IPR016187 CTDL_fold
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003599 Ig_sub
IPR013106 Ig_V-set
IPR000538 Link_dom
IPR035976 Sushi/SCR/CCP_sf
IPR000436 Sushi_SCR_CCP_dom
PfamiView protein in Pfam
PF00008 EGF, 1 hit
PF00059 Lectin_C, 1 hit
PF00084 Sushi, 1 hit
PF07686 V-set, 1 hit
PF00193 Xlink, 2 hits
PRINTSiPR01265 LINKMODULE
SMARTiView protein in SMART
SM00032 CCP, 1 hit
SM00034 CLECT, 1 hit
SM00181 EGF, 2 hits
SM00179 EGF_CA, 2 hits
SM00409 IG, 1 hit
SM00445 LINK, 2 hits
SUPFAMiSSF48726 SSF48726, 1 hit
SSF56436 SSF56436, 3 hits
SSF57535 SSF57535, 1 hit
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 1 hit
PS00615 C_TYPE_LECTIN_1, 1 hit
PS50041 C_TYPE_LECTIN_2, 1 hit
PS00022 EGF_1, 3 hits
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 2 hits
PS01187 EGF_CA, 1 hit
PS50835 IG_LIKE, 1 hit
PS01241 LINK_1, 2 hits
PS50963 LINK_2, 2 hits
PS50923 SUSHI, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O14594-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAPFVWALG LLMLQMLLFV AGEQGTQDIT DASERGLHMQ KLGSGSVQAA
60 70 80 90 100
LAELVALPCL FTLQPRPSAA RDAPRIKWTK VRTASGQRQD LPILVAKDNV
110 120 130 140 150
VRVAKSWQGR VSLPSYPRRR ANATLLLGPL RASDSGLYRC QVVRGIEDEQ
160 170 180 190 200
DLVPLEVTGV VFHYRSARDR YALTFAEAQE ACRLSSAIIA APRHLQAAFE
210 220 230 240 250
DGFDNCDAGW LSDRTVRYPI TQSRPGCYGD RSSLPGVRSY GRRNPQELYD
260 270 280 290 300
VYCFARELGG EVFYVGPARR LTLAGARAQC RRQGAALASV GQLHLAWHEG
310 320 330 340 350
LDQCDPGWLA DGSVRYPIQT PRRRCGGPAP GVRTVYRFAN RTGFPSPAER
360 370 380 390 400
FDAYCFRAHH PTSQHGDLET PSSGDEGEIL SAEGPPVREL EPTLEEEEVV
410 420 430 440 450
TPDFQEPLVS SGEEETLILE EKQESQQTLS PTPGDPMLAS WPTGEVWLST
460 470 480 490 500
VAPSPSDMGA GTAASSHTEV APTDPMPRRR GRFKGLNGRY FQQQEPEPGL
510 520 530 540 550
QGGMEASAQP PTSEAAVNQM EPPLAMAVTE MLGSGQSRSP WADLTNEVDM
560 570 580 590 600
PGAGSAGGKS SPEPWLWPPT MVPPSISGHS RAPVLELEKA EGPSARPATP
610 620 630 640 650
DLFWSPLEAT VSAPSPAPWE AFPVATSPDL PMMAMLRGPK EWMLPHPTPI
660 670 680 690 700
STEANRVEAH GEATATAPPS PAAETKVYSL PLSLTPTGQG GEAMPTTPES
710 720 730 740 750
PRADFRETGE TSPAQVNKAE HSSSSPWPSV NRNVAVGFVP TETATEPTGL
760 770 780 790 800
RGIPGSESGV FDTAESPTSG LQATVDEVQD PWPSVYSKGL DASSPSAPLG
810 820 830 840 850
SPGVFLVPKV TPNLEPWVAT DEGPTVNPMD STVTPAPSDA SGIWEPGSQV
860 870 880 890 900
FEEAESTTLS PQVALDTSIV TPLTTLEQGD KVGVPAMSTL GSSSSQPHPE
910 920 930 940 950
PEDQVETQGT SGASVPPHQS SPLGKPAVPP GTPTAASVGE SASVSSGEPT
960 970 980 990 1000
VPWDPSSTLL PVTLGIEDFE LEVLAGSPGV ESFWEEVASG EEPALPGTPM
1010 1020 1030 1040 1050
NAGAEEVHSD PCENNPCLHG GTCNANGTMY GCSCDQGFAG ENCEIDIDDC
1060 1070 1080 1090 1100
LCSPCENGGT CIDEVNGFVC LCLPSYGGSF CEKDTEGCDR GWHKFQGHCY
1110 1120 1130 1140 1150
RYFAHRRAWE DAEKDCRRRS GHLTSVHSPE EHSFINSFGH ENTWIGLNDR
1160 1170 1180 1190 1200
IVERDFQWTD NTGLQFENWR ENQPDNFFAG GEDCVVMVAH ESGRWNDVPC
1210 1220 1230 1240 1250
NYNLPYVCKK GTVLCGPPPA VENASLIGAR KAKYNVHATV RYQCNEGFAQ
1260 1270 1280 1290 1300
HHVATIRCRS NGKWDRPQIV CTKPRRSHRM RRHHHHHQHH HQHHHHKSRK
1310 1320
ERRKHKKHPT EDWEKDEGNF C
Length:1,321
Mass (Da):143,093
Last modified:November 13, 2007 - v3
Checksum:i22E21FFA24CE9FB2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1234Y → N in AAC80576 (PubMed:9795216).Curated1
Sequence conflicti1282R → G in AAC80576 (PubMed:9795216).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02452170A → T. Corresponds to variant dbSNP:rs2228601Ensembl.1
Natural variantiVAR_02021392P → S. Corresponds to variant dbSNP:rs2228603Ensembl.1
Natural variantiVAR_0161761254A → V1 PublicationCorresponds to variant dbSNP:rs1064389Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026547 mRNA Translation: AAC80576.1
AC003110 Genomic DNA Translation: AAB86655.1
AC005254 Genomic DNA Translation: AAC25581.1
CCDSiCCDS12397.1
RefSeqiNP_004377.2, NM_004386.2
UniGeneiHs.169047

Genome annotation databases

EnsembliENST00000252575; ENSP00000252575; ENSG00000130287
GeneIDi1463
KEGGihsa:1463
UCSCiuc002nlz.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiNCAN_HUMAN
AccessioniPrimary (citable) accession number: O14594
Secondary accession number(s): Q9UPK6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: November 13, 2007
Last modified: May 23, 2018
This is version 164 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health