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O14594

- NCAN_HUMAN

UniProt

O14594 - NCAN_HUMAN

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Protein

Neurocan core protein

Gene

NCAN

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. carbohydrate binding Source: UniProtKB-KW
  3. hyaluronic acid binding Source: UniProtKB-KW

GO - Biological processi

  1. axon guidance Source: Reactome
  2. carbohydrate metabolic process Source: Reactome
  3. cell adhesion Source: UniProtKB-KW
  4. chondroitin sulfate biosynthetic process Source: Reactome
  5. chondroitin sulfate catabolic process Source: Reactome
  6. chondroitin sulfate metabolic process Source: Reactome
  7. dermatan sulfate biosynthetic process Source: Reactome
  8. extracellular matrix organization Source: Reactome
  9. glycosaminoglycan metabolic process Source: Reactome
  10. regulation of synapse structural plasticity Source: Ensembl
  11. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium, Hyaluronic acid, Lectin

Enzyme and pathway databases

ReactomeiREACT_120800. Dermatan sulfate biosynthesis.
REACT_120888. CS/DS degradation.
REACT_120989. Chondroitin sulfate biosynthesis.
REACT_121408. A tetrasaccharide linker sequence is required for GAG synthesis.
REACT_163906. ECM proteoglycans.
REACT_18312. NCAM1 interactions.
REACT_22205. L1CAM interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Neurocan core protein
Alternative name(s):
Chondroitin sulfate proteoglycan 3
Gene namesi
Name:NCAN
Synonyms:CSPG3, NEUR
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:2465. NCAN.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: Reactome
  2. Golgi lumen Source: Reactome
  3. lysosomal lumen Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162396986.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 13211299Neurocan core proteinPRO_0000017516Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi59 ↔ 140By similarity
Glycosylationi122 – 1221N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi182 ↔ 253By similarity
Disulfide bondi206 ↔ 227By similarity
Disulfide bondi280 ↔ 355By similarity
Disulfide bondi304 ↔ 325By similarity
Glycosylationi340 – 3401N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi1012 ↔ 1023By similarity
Disulfide bondi1017 ↔ 1032By similarity
Glycosylationi1026 – 10261N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi1034 ↔ 1043By similarity
Disulfide bondi1050 ↔ 1061By similarity
Disulfide bondi1055 ↔ 1070By similarity
Disulfide bondi1072 ↔ 1081By similarity
Disulfide bondi1088 ↔ 1099By similarity
Disulfide bondi1116 ↔ 1208By similarity
Disulfide bondi1184 ↔ 1200By similarity
Disulfide bondi1215 ↔ 1258By similarity
Glycosylationi1223 – 12231N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi1244 ↔ 1271By similarity

Post-translational modificationi

O-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Proteoglycan

Proteomic databases

PaxDbiO14594.
PRIDEiO14594.

PTM databases

PhosphoSiteiO14594.

Expressioni

Tissue specificityi

Brain.

Gene expression databases

BgeeiO14594.
CleanExiHS_NCAN.
ExpressionAtlasiO14594. baseline and differential.
GenevestigatoriO14594.

Organism-specific databases

HPAiHPA036814.

Interactioni

Protein-protein interaction databases

BioGridi107845. 6 interactions.
IntActiO14594. 1 interaction.
STRINGi9606.ENSP00000252575.

Structurei

3D structure databases

ProteinModelPortaliO14594.
SMRiO14594. Positions 40-149, 161-254, 270-359, 1007-1082, 1086-1211.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 153116Ig-like V-typeAdd
BLAST
Domaini160 – 25596Link 1PROSITE-ProRule annotationAdd
BLAST
Domaini259 – 35799Link 2PROSITE-ProRule annotationAdd
BLAST
Domaini1008 – 104437EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini1046 – 108237EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini1084 – 1213130C-type lectinPROSITE-ProRule annotationAdd
BLAST
Domaini1213 – 127361SushiPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni708 – 7125O-glycosylated at one site

Sequence similaritiesi

Contains 1 C-type lectin domain.PROSITE-ProRule annotation
Contains 2 EGF-like domains.PROSITE-ProRule annotation
Contains 2 Link domains.PROSITE-ProRule annotation
Contains 1 Sushi (CCP/SCR) domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Immunoglobulin domain, Repeat, Signal, Sushi

Phylogenomic databases

eggNOGiNOG147231.
GeneTreeiENSGT00760000119025.
HOVERGENiHBG078994.
InParanoidiO14594.
KOiK06794.
OMAiCSPCENG.
OrthoDBiEOG7FFMQR.
PhylomeDBiO14594.
TreeFamiTF332134.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.10.100.10. 3 hits.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR000538. Link.
IPR000436. Sushi_SCR_CCP.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
PF00084. Sushi. 1 hit.
PF07686. V-set. 1 hit.
PF00193. Xlink. 2 hits.
[Graphical view]
PRINTSiPR01265. LINKMODULE.
SMARTiSM00032. CCP. 1 hit.
SM00034. CLECT. 1 hit.
SM00181. EGF. 1 hit.
SM00179. EGF_CA. 1 hit.
SM00409. IG. 1 hit.
SM00445. LINK. 2 hits.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 3 hits.
SSF57535. SSF57535. 1 hit.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
PS00022. EGF_1. 3 hits.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 1 hit.
PS50835. IG_LIKE. 1 hit.
PS01241. LINK_1. 2 hits.
PS50963. LINK_2. 2 hits.
PS50923. SUSHI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O14594-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGAPFVWALG LLMLQMLLFV AGEQGTQDIT DASERGLHMQ KLGSGSVQAA
60 70 80 90 100
LAELVALPCL FTLQPRPSAA RDAPRIKWTK VRTASGQRQD LPILVAKDNV
110 120 130 140 150
VRVAKSWQGR VSLPSYPRRR ANATLLLGPL RASDSGLYRC QVVRGIEDEQ
160 170 180 190 200
DLVPLEVTGV VFHYRSARDR YALTFAEAQE ACRLSSAIIA APRHLQAAFE
210 220 230 240 250
DGFDNCDAGW LSDRTVRYPI TQSRPGCYGD RSSLPGVRSY GRRNPQELYD
260 270 280 290 300
VYCFARELGG EVFYVGPARR LTLAGARAQC RRQGAALASV GQLHLAWHEG
310 320 330 340 350
LDQCDPGWLA DGSVRYPIQT PRRRCGGPAP GVRTVYRFAN RTGFPSPAER
360 370 380 390 400
FDAYCFRAHH PTSQHGDLET PSSGDEGEIL SAEGPPVREL EPTLEEEEVV
410 420 430 440 450
TPDFQEPLVS SGEEETLILE EKQESQQTLS PTPGDPMLAS WPTGEVWLST
460 470 480 490 500
VAPSPSDMGA GTAASSHTEV APTDPMPRRR GRFKGLNGRY FQQQEPEPGL
510 520 530 540 550
QGGMEASAQP PTSEAAVNQM EPPLAMAVTE MLGSGQSRSP WADLTNEVDM
560 570 580 590 600
PGAGSAGGKS SPEPWLWPPT MVPPSISGHS RAPVLELEKA EGPSARPATP
610 620 630 640 650
DLFWSPLEAT VSAPSPAPWE AFPVATSPDL PMMAMLRGPK EWMLPHPTPI
660 670 680 690 700
STEANRVEAH GEATATAPPS PAAETKVYSL PLSLTPTGQG GEAMPTTPES
710 720 730 740 750
PRADFRETGE TSPAQVNKAE HSSSSPWPSV NRNVAVGFVP TETATEPTGL
760 770 780 790 800
RGIPGSESGV FDTAESPTSG LQATVDEVQD PWPSVYSKGL DASSPSAPLG
810 820 830 840 850
SPGVFLVPKV TPNLEPWVAT DEGPTVNPMD STVTPAPSDA SGIWEPGSQV
860 870 880 890 900
FEEAESTTLS PQVALDTSIV TPLTTLEQGD KVGVPAMSTL GSSSSQPHPE
910 920 930 940 950
PEDQVETQGT SGASVPPHQS SPLGKPAVPP GTPTAASVGE SASVSSGEPT
960 970 980 990 1000
VPWDPSSTLL PVTLGIEDFE LEVLAGSPGV ESFWEEVASG EEPALPGTPM
1010 1020 1030 1040 1050
NAGAEEVHSD PCENNPCLHG GTCNANGTMY GCSCDQGFAG ENCEIDIDDC
1060 1070 1080 1090 1100
LCSPCENGGT CIDEVNGFVC LCLPSYGGSF CEKDTEGCDR GWHKFQGHCY
1110 1120 1130 1140 1150
RYFAHRRAWE DAEKDCRRRS GHLTSVHSPE EHSFINSFGH ENTWIGLNDR
1160 1170 1180 1190 1200
IVERDFQWTD NTGLQFENWR ENQPDNFFAG GEDCVVMVAH ESGRWNDVPC
1210 1220 1230 1240 1250
NYNLPYVCKK GTVLCGPPPA VENASLIGAR KAKYNVHATV RYQCNEGFAQ
1260 1270 1280 1290 1300
HHVATIRCRS NGKWDRPQIV CTKPRRSHRM RRHHHHHQHH HQHHHHKSRK
1310 1320
ERRKHKKHPT EDWEKDEGNF C
Length:1,321
Mass (Da):143,093
Last modified:November 13, 2007 - v3
Checksum:i22E21FFA24CE9FB2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1234 – 12341Y → N in AAC80576. (PubMed:9795216)Curated
Sequence conflicti1282 – 12821R → G in AAC80576. (PubMed:9795216)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti70 – 701A → T.
Corresponds to variant rs2228601 [ dbSNP | Ensembl ].
VAR_024521
Natural varianti92 – 921P → S.
Corresponds to variant rs2228603 [ dbSNP | Ensembl ].
VAR_020213
Natural varianti1254 – 12541A → V.1 Publication
Corresponds to variant rs1064389 [ dbSNP | Ensembl ].
VAR_016176

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF026547 mRNA. Translation: AAC80576.1.
AC003110 Genomic DNA. Translation: AAB86655.1.
AC005254 Genomic DNA. Translation: AAC25581.1.
CCDSiCCDS12397.1.
RefSeqiNP_004377.2. NM_004386.2.
XP_005259802.1. XM_005259745.1.
XP_005259803.1. XM_005259746.1.
UniGeneiHs.169047.

Genome annotation databases

EnsembliENST00000252575; ENSP00000252575; ENSG00000130287.
GeneIDi1463.
KEGGihsa:1463.
UCSCiuc002nlz.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Neurocan

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF026547 mRNA. Translation: AAC80576.1 .
AC003110 Genomic DNA. Translation: AAB86655.1 .
AC005254 Genomic DNA. Translation: AAC25581.1 .
CCDSi CCDS12397.1.
RefSeqi NP_004377.2. NM_004386.2.
XP_005259802.1. XM_005259745.1.
XP_005259803.1. XM_005259746.1.
UniGenei Hs.169047.

3D structure databases

ProteinModelPortali O14594.
SMRi O14594. Positions 40-149, 161-254, 270-359, 1007-1082, 1086-1211.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 107845. 6 interactions.
IntActi O14594. 1 interaction.
STRINGi 9606.ENSP00000252575.

Chemistry

DrugBanki DB08818. Hyaluronan.

PTM databases

PhosphoSitei O14594.

Proteomic databases

PaxDbi O14594.
PRIDEi O14594.

Protocols and materials databases

DNASUi 1463.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000252575 ; ENSP00000252575 ; ENSG00000130287 .
GeneIDi 1463.
KEGGi hsa:1463.
UCSCi uc002nlz.3. human.

Organism-specific databases

CTDi 1463.
GeneCardsi GC19P019322.
H-InvDB HIX0027449.
HGNCi HGNC:2465. NCAN.
HPAi HPA036814.
MIMi 600826. gene.
neXtProti NX_O14594.
PharmGKBi PA162396986.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG147231.
GeneTreei ENSGT00760000119025.
HOVERGENi HBG078994.
InParanoidi O14594.
KOi K06794.
OMAi CSPCENG.
OrthoDBi EOG7FFMQR.
PhylomeDBi O14594.
TreeFami TF332134.

Enzyme and pathway databases

Reactomei REACT_120800. Dermatan sulfate biosynthesis.
REACT_120888. CS/DS degradation.
REACT_120989. Chondroitin sulfate biosynthesis.
REACT_121408. A tetrasaccharide linker sequence is required for GAG synthesis.
REACT_163906. ECM proteoglycans.
REACT_18312. NCAM1 interactions.
REACT_22205. L1CAM interactions.

Miscellaneous databases

GeneWikii Neurocan.
GenomeRNAii 1463.
NextBioi 6009.
PROi O14594.
SOURCEi Search...

Gene expression databases

Bgeei O14594.
CleanExi HS_NCAN.
ExpressionAtlasi O14594. baseline and differential.
Genevestigatori O14594.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
3.10.100.10. 3 hits.
InterProi IPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR000538. Link.
IPR000436. Sushi_SCR_CCP.
[Graphical view ]
Pfami PF00059. Lectin_C. 1 hit.
PF00084. Sushi. 1 hit.
PF07686. V-set. 1 hit.
PF00193. Xlink. 2 hits.
[Graphical view ]
PRINTSi PR01265. LINKMODULE.
SMARTi SM00032. CCP. 1 hit.
SM00034. CLECT. 1 hit.
SM00181. EGF. 1 hit.
SM00179. EGF_CA. 1 hit.
SM00409. IG. 1 hit.
SM00445. LINK. 2 hits.
[Graphical view ]
SUPFAMi SSF56436. SSF56436. 3 hits.
SSF57535. SSF57535. 1 hit.
PROSITEi PS00010. ASX_HYDROXYL. 1 hit.
PS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
PS00022. EGF_1. 3 hits.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 1 hit.
PS50835. IG_LIKE. 1 hit.
PS01241. LINK_1. 2 hits.
PS50963. LINK_2. 2 hits.
PS50923. SUSHI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the human neurocan gene, CSPG3."
    Prange C.K., Pennacchio L.A., Lieuallen K., Fan W., Lennon G.G.
    Gene 221:199-205(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-1254.
  2. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."
    Halim A., Ruetschi U., Larson G., Nilsson J.
    J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiNCAN_HUMAN
AccessioniPrimary (citable) accession number: O14594
Secondary accession number(s): Q9UPK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: November 13, 2007
Last modified: October 29, 2014
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3