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O14594

- NCAN_HUMAN

UniProt

O14594 - NCAN_HUMAN

Protein

Neurocan core protein

Gene

NCAN

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 3 (13 Nov 2007)
      Previous versions | rss
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    Functioni

    May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. carbohydrate binding Source: InterPro
    3. hyaluronic acid binding Source: UniProtKB-KW

    GO - Biological processi

    1. axon guidance Source: Reactome
    2. carbohydrate metabolic process Source: Reactome
    3. cell adhesion Source: UniProtKB-KW
    4. chondroitin sulfate biosynthetic process Source: Reactome
    5. chondroitin sulfate catabolic process Source: Reactome
    6. chondroitin sulfate metabolic process Source: Reactome
    7. dermatan sulfate biosynthetic process Source: Reactome
    8. extracellular matrix organization Source: Reactome
    9. glycosaminoglycan metabolic process Source: Reactome
    10. regulation of synapse structural plasticity Source: Ensembl
    11. small molecule metabolic process Source: Reactome

    Keywords - Biological processi

    Cell adhesion

    Keywords - Ligandi

    Calcium, Hyaluronic acid, Lectin

    Enzyme and pathway databases

    ReactomeiREACT_120800. Dermatan sulfate biosynthesis.
    REACT_120888. CS/DS degradation.
    REACT_120989. Chondroitin sulfate biosynthesis.
    REACT_121408. A tetrasaccharide linker sequence is required for GAG synthesis.
    REACT_163906. ECM proteoglycans.
    REACT_18312. NCAM1 interactions.
    REACT_22205. L1CAM interactions.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neurocan core protein
    Alternative name(s):
    Chondroitin sulfate proteoglycan 3
    Gene namesi
    Name:NCAN
    Synonyms:CSPG3, NEUR
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:2465. NCAN.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: Reactome
    2. Golgi lumen Source: Reactome
    3. lysosomal lumen Source: Reactome

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162396986.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 13211299Neurocan core proteinPRO_0000017516Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi59 ↔ 140By similarity
    Glycosylationi122 – 1221N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi182 ↔ 253By similarity
    Disulfide bondi206 ↔ 227By similarity
    Disulfide bondi280 ↔ 355By similarity
    Disulfide bondi304 ↔ 325By similarity
    Glycosylationi340 – 3401N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi1012 ↔ 1023By similarity
    Disulfide bondi1017 ↔ 1032By similarity
    Glycosylationi1026 – 10261N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi1034 ↔ 1043By similarity
    Disulfide bondi1050 ↔ 1061By similarity
    Disulfide bondi1055 ↔ 1070By similarity
    Disulfide bondi1072 ↔ 1081By similarity
    Disulfide bondi1088 ↔ 1099By similarity
    Disulfide bondi1116 ↔ 1208By similarity
    Disulfide bondi1184 ↔ 1200By similarity
    Disulfide bondi1215 ↔ 1258By similarity
    Glycosylationi1223 – 12231N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi1244 ↔ 1271By similarity

    Post-translational modificationi

    O-glycosylated.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Proteoglycan

    Proteomic databases

    PaxDbiO14594.
    PRIDEiO14594.

    PTM databases

    PhosphoSiteiO14594.

    Expressioni

    Tissue specificityi

    Brain.

    Gene expression databases

    ArrayExpressiO14594.
    BgeeiO14594.
    CleanExiHS_NCAN.
    GenevestigatoriO14594.

    Organism-specific databases

    HPAiHPA036814.

    Interactioni

    Protein-protein interaction databases

    BioGridi107845. 6 interactions.
    IntActiO14594. 1 interaction.
    STRINGi9606.ENSP00000252575.

    Structurei

    3D structure databases

    ProteinModelPortaliO14594.
    SMRiO14594. Positions 39-148, 161-254, 270-359, 986-1083, 1086-1211.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini38 – 153116Ig-like V-typeAdd
    BLAST
    Domaini160 – 25596Link 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini259 – 35799Link 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1008 – 104437EGF-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1046 – 108237EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini1084 – 1213130C-type lectinPROSITE-ProRule annotationAdd
    BLAST
    Domaini1213 – 127361SushiPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni708 – 7125O-glycosylated at one site

    Sequence similaritiesi

    Contains 1 C-type lectin domain.PROSITE-ProRule annotation
    Contains 2 EGF-like domains.PROSITE-ProRule annotation
    Contains 2 Link domains.PROSITE-ProRule annotation
    Contains 1 Sushi (CCP/SCR) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Immunoglobulin domain, Repeat, Signal, Sushi

    Phylogenomic databases

    eggNOGiNOG147231.
    HOVERGENiHBG078994.
    InParanoidiO14594.
    KOiK06794.
    OMAiCSPCENG.
    OrthoDBiEOG7FFMQR.
    PhylomeDBiO14594.
    TreeFamiTF332134.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    3.10.100.10. 3 hits.
    InterProiIPR001304. C-type_lectin.
    IPR016186. C-type_lectin-like.
    IPR018378. C-type_lectin_CS.
    IPR016187. C-type_lectin_fold.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
    IPR018097. EGF_Ca-bd_CS.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    IPR000538. Link.
    IPR000436. Sushi_SCR_CCP.
    [Graphical view]
    PfamiPF00059. Lectin_C. 1 hit.
    PF00084. Sushi. 1 hit.
    PF07686. V-set. 1 hit.
    PF00193. Xlink. 2 hits.
    [Graphical view]
    PRINTSiPR01265. LINKMODULE.
    SMARTiSM00032. CCP. 1 hit.
    SM00034. CLECT. 1 hit.
    SM00181. EGF. 1 hit.
    SM00179. EGF_CA. 1 hit.
    SM00409. IG. 1 hit.
    SM00445. LINK. 2 hits.
    [Graphical view]
    SUPFAMiSSF56436. SSF56436. 3 hits.
    SSF57535. SSF57535. 1 hit.
    PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
    PS00615. C_TYPE_LECTIN_1. 1 hit.
    PS50041. C_TYPE_LECTIN_2. 1 hit.
    PS00022. EGF_1. 3 hits.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 2 hits.
    PS01187. EGF_CA. 1 hit.
    PS50835. IG_LIKE. 1 hit.
    PS01241. LINK_1. 2 hits.
    PS50963. LINK_2. 2 hits.
    PS50923. SUSHI. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O14594-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGAPFVWALG LLMLQMLLFV AGEQGTQDIT DASERGLHMQ KLGSGSVQAA     50
    LAELVALPCL FTLQPRPSAA RDAPRIKWTK VRTASGQRQD LPILVAKDNV 100
    VRVAKSWQGR VSLPSYPRRR ANATLLLGPL RASDSGLYRC QVVRGIEDEQ 150
    DLVPLEVTGV VFHYRSARDR YALTFAEAQE ACRLSSAIIA APRHLQAAFE 200
    DGFDNCDAGW LSDRTVRYPI TQSRPGCYGD RSSLPGVRSY GRRNPQELYD 250
    VYCFARELGG EVFYVGPARR LTLAGARAQC RRQGAALASV GQLHLAWHEG 300
    LDQCDPGWLA DGSVRYPIQT PRRRCGGPAP GVRTVYRFAN RTGFPSPAER 350
    FDAYCFRAHH PTSQHGDLET PSSGDEGEIL SAEGPPVREL EPTLEEEEVV 400
    TPDFQEPLVS SGEEETLILE EKQESQQTLS PTPGDPMLAS WPTGEVWLST 450
    VAPSPSDMGA GTAASSHTEV APTDPMPRRR GRFKGLNGRY FQQQEPEPGL 500
    QGGMEASAQP PTSEAAVNQM EPPLAMAVTE MLGSGQSRSP WADLTNEVDM 550
    PGAGSAGGKS SPEPWLWPPT MVPPSISGHS RAPVLELEKA EGPSARPATP 600
    DLFWSPLEAT VSAPSPAPWE AFPVATSPDL PMMAMLRGPK EWMLPHPTPI 650
    STEANRVEAH GEATATAPPS PAAETKVYSL PLSLTPTGQG GEAMPTTPES 700
    PRADFRETGE TSPAQVNKAE HSSSSPWPSV NRNVAVGFVP TETATEPTGL 750
    RGIPGSESGV FDTAESPTSG LQATVDEVQD PWPSVYSKGL DASSPSAPLG 800
    SPGVFLVPKV TPNLEPWVAT DEGPTVNPMD STVTPAPSDA SGIWEPGSQV 850
    FEEAESTTLS PQVALDTSIV TPLTTLEQGD KVGVPAMSTL GSSSSQPHPE 900
    PEDQVETQGT SGASVPPHQS SPLGKPAVPP GTPTAASVGE SASVSSGEPT 950
    VPWDPSSTLL PVTLGIEDFE LEVLAGSPGV ESFWEEVASG EEPALPGTPM 1000
    NAGAEEVHSD PCENNPCLHG GTCNANGTMY GCSCDQGFAG ENCEIDIDDC 1050
    LCSPCENGGT CIDEVNGFVC LCLPSYGGSF CEKDTEGCDR GWHKFQGHCY 1100
    RYFAHRRAWE DAEKDCRRRS GHLTSVHSPE EHSFINSFGH ENTWIGLNDR 1150
    IVERDFQWTD NTGLQFENWR ENQPDNFFAG GEDCVVMVAH ESGRWNDVPC 1200
    NYNLPYVCKK GTVLCGPPPA VENASLIGAR KAKYNVHATV RYQCNEGFAQ 1250
    HHVATIRCRS NGKWDRPQIV CTKPRRSHRM RRHHHHHQHH HQHHHHKSRK 1300
    ERRKHKKHPT EDWEKDEGNF C 1321
    Length:1,321
    Mass (Da):143,093
    Last modified:November 13, 2007 - v3
    Checksum:i22E21FFA24CE9FB2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1234 – 12341Y → N in AAC80576. (PubMed:9795216)Curated
    Sequence conflicti1282 – 12821R → G in AAC80576. (PubMed:9795216)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti70 – 701A → T.
    Corresponds to variant rs2228601 [ dbSNP | Ensembl ].
    VAR_024521
    Natural varianti92 – 921P → S.
    Corresponds to variant rs2228603 [ dbSNP | Ensembl ].
    VAR_020213
    Natural varianti1254 – 12541A → V.1 Publication
    Corresponds to variant rs1064389 [ dbSNP | Ensembl ].
    VAR_016176

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF026547 mRNA. Translation: AAC80576.1.
    AC003110 Genomic DNA. Translation: AAB86655.1.
    AC005254 Genomic DNA. Translation: AAC25581.1.
    CCDSiCCDS12397.1.
    RefSeqiNP_004377.2. NM_004386.2.
    XP_005259802.1. XM_005259745.1.
    XP_005259803.1. XM_005259746.1.
    UniGeneiHs.169047.

    Genome annotation databases

    EnsembliENST00000252575; ENSP00000252575; ENSG00000130287.
    GeneIDi1463.
    KEGGihsa:1463.
    UCSCiuc002nlz.3. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - Glycan Binding

    Neurocan

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF026547 mRNA. Translation: AAC80576.1 .
    AC003110 Genomic DNA. Translation: AAB86655.1 .
    AC005254 Genomic DNA. Translation: AAC25581.1 .
    CCDSi CCDS12397.1.
    RefSeqi NP_004377.2. NM_004386.2.
    XP_005259802.1. XM_005259745.1.
    XP_005259803.1. XM_005259746.1.
    UniGenei Hs.169047.

    3D structure databases

    ProteinModelPortali O14594.
    SMRi O14594. Positions 39-148, 161-254, 270-359, 986-1083, 1086-1211.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107845. 6 interactions.
    IntActi O14594. 1 interaction.
    STRINGi 9606.ENSP00000252575.

    PTM databases

    PhosphoSitei O14594.

    Proteomic databases

    PaxDbi O14594.
    PRIDEi O14594.

    Protocols and materials databases

    DNASUi 1463.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000252575 ; ENSP00000252575 ; ENSG00000130287 .
    GeneIDi 1463.
    KEGGi hsa:1463.
    UCSCi uc002nlz.3. human.

    Organism-specific databases

    CTDi 1463.
    GeneCardsi GC19P019322.
    H-InvDB HIX0027449.
    HGNCi HGNC:2465. NCAN.
    HPAi HPA036814.
    MIMi 600826. gene.
    neXtProti NX_O14594.
    PharmGKBi PA162396986.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG147231.
    HOVERGENi HBG078994.
    InParanoidi O14594.
    KOi K06794.
    OMAi CSPCENG.
    OrthoDBi EOG7FFMQR.
    PhylomeDBi O14594.
    TreeFami TF332134.

    Enzyme and pathway databases

    Reactomei REACT_120800. Dermatan sulfate biosynthesis.
    REACT_120888. CS/DS degradation.
    REACT_120989. Chondroitin sulfate biosynthesis.
    REACT_121408. A tetrasaccharide linker sequence is required for GAG synthesis.
    REACT_163906. ECM proteoglycans.
    REACT_18312. NCAM1 interactions.
    REACT_22205. L1CAM interactions.

    Miscellaneous databases

    GeneWikii Neurocan.
    GenomeRNAii 1463.
    NextBioi 6009.
    PROi O14594.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O14594.
    Bgeei O14594.
    CleanExi HS_NCAN.
    Genevestigatori O14594.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    3.10.100.10. 3 hits.
    InterProi IPR001304. C-type_lectin.
    IPR016186. C-type_lectin-like.
    IPR018378. C-type_lectin_CS.
    IPR016187. C-type_lectin_fold.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
    IPR018097. EGF_Ca-bd_CS.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    IPR000538. Link.
    IPR000436. Sushi_SCR_CCP.
    [Graphical view ]
    Pfami PF00059. Lectin_C. 1 hit.
    PF00084. Sushi. 1 hit.
    PF07686. V-set. 1 hit.
    PF00193. Xlink. 2 hits.
    [Graphical view ]
    PRINTSi PR01265. LINKMODULE.
    SMARTi SM00032. CCP. 1 hit.
    SM00034. CLECT. 1 hit.
    SM00181. EGF. 1 hit.
    SM00179. EGF_CA. 1 hit.
    SM00409. IG. 1 hit.
    SM00445. LINK. 2 hits.
    [Graphical view ]
    SUPFAMi SSF56436. SSF56436. 3 hits.
    SSF57535. SSF57535. 1 hit.
    PROSITEi PS00010. ASX_HYDROXYL. 1 hit.
    PS00615. C_TYPE_LECTIN_1. 1 hit.
    PS50041. C_TYPE_LECTIN_2. 1 hit.
    PS00022. EGF_1. 3 hits.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 2 hits.
    PS01187. EGF_CA. 1 hit.
    PS50835. IG_LIKE. 1 hit.
    PS01241. LINK_1. 2 hits.
    PS50963. LINK_2. 2 hits.
    PS50923. SUSHI. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the human neurocan gene, CSPG3."
      Prange C.K., Pennacchio L.A., Lieuallen K., Fan W., Lennon G.G.
      Gene 221:199-205(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-1254.
    2. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."
      Halim A., Ruetschi U., Larson G., Nilsson J.
      J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiNCAN_HUMAN
    AccessioniPrimary (citable) accession number: O14594
    Secondary accession number(s): Q9UPK6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 27, 2003
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 134 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3