O14594 (NCAN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neurocan core protein

Alternative name(s):
Chondroitin sulfate proteoglycan 3

Gene names

Name:	NCAN
Synonyms:	CSPG3, NEUR

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.
Subcellular location	Secreted By similarity.
Tissue specificity	Brain.
Post-translational modification	O-glycosylated. Ref.3
Sequence similarities	Belongs to the aggrecan/versican proteoglycan family. Contains 1 C-type lectin domain. Contains 2 EGF-like domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain. Contains 2 Link domains. Contains 1 Sushi (CCP/SCR) domain.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	EGF-like domain Immunoglobulin domain Repeat Signal Sushi
Ligand	Calcium Hyaluronic acid Lectin
PTM	Disulfide bond Glycoprotein Proteoglycan
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axon guidance Traceable author statement. Source: Reactome carbohydrate metabolic process Traceable author statement. Source: Reactome cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW chondroitin sulfate biosynthetic process Traceable author statement. Source: Reactome chondroitin sulfate catabolic process Traceable author statement. Source: Reactome dermatan sulfate biosynthetic process Traceable author statement. Source: Reactome regulation of synapse structural plasticity Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi lumen Traceable author statement. Source: Reactome extracellular region Traceable author statement. Source: Reactome lysosomal lumen Traceable author statement. Source: Reactome
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro carbohydrate binding Inferred from electronic annotation. Source: InterPro hyaluronic acid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 1321	1299	Neurocan core protein		PRO_0000017516
Regions
	Domain	38 – 153	116	Ig-like V-type
	Domain	160 – 255	96	Link 1
	Domain	259 – 357	99	Link 2
	Domain	1008 – 1044	37	EGF-like 1
	Domain	1046 – 1082	37	EGF-like 2; calcium-binding Potential
	Domain	1084 – 1213	130	C-type lectin
	Domain	1213 – 1273	61	Sushi
	Region	708 – 712	5	O-glycosylated at one site
Amino acid modifications
	Glycosylation	122	1	N-linked (GlcNAc...) Potential
	Glycosylation	340	1	N-linked (GlcNAc...) Potential
	Glycosylation	1026	1	N-linked (GlcNAc...) Potential
	Glycosylation	1223	1	N-linked (GlcNAc...) Potential
	Disulfide bond	59 ↔ 140		By similarity
	Disulfide bond	182 ↔ 253		By similarity
	Disulfide bond	206 ↔ 227		By similarity
	Disulfide bond	280 ↔ 355		By similarity
	Disulfide bond	304 ↔ 325		By similarity
	Disulfide bond	1012 ↔ 1023		By similarity
	Disulfide bond	1017 ↔ 1032		By similarity
	Disulfide bond	1034 ↔ 1043		By similarity
	Disulfide bond	1050 ↔ 1061		By similarity
	Disulfide bond	1055 ↔ 1070		By similarity
	Disulfide bond	1072 ↔ 1081		By similarity
	Disulfide bond	1088 ↔ 1099		By similarity
	Disulfide bond	1116 ↔ 1208		By similarity
	Disulfide bond	1184 ↔ 1200		By similarity
	Disulfide bond	1215 ↔ 1258		By similarity
	Disulfide bond	1244 ↔ 1271		By similarity
Natural variations
	Natural variant	70	1	A → T. Corresponds to variant rs2228601 [ dbSNP | Ensembl ].		VAR_024521
	Natural variant	92	1	P → S. Corresponds to variant rs2228603 [ dbSNP | Ensembl ].		VAR_020213
	Natural variant	1254	1	A → V. Ref.1 Corresponds to variant rs1064389 [ dbSNP | Ensembl ].		VAR_016176
Experimental info
	Sequence conflict	1234	1	Y → N in AAC80576. Ref.1
	Sequence conflict	1282	1	R → G in AAC80576. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O14594 [UniParc]. Last modified November 13, 2007. Version 3. Checksum: 22E21FFA24CE9FB2	FASTA	1,321	143,093
	10 20 30 40 50 60 MGAPFVWALG LLMLQMLLFV AGEQGTQDIT DASERGLHMQ KLGSGSVQAA LAELVALPCL 70 80 90 100 110 120 FTLQPRPSAA RDAPRIKWTK VRTASGQRQD LPILVAKDNV VRVAKSWQGR VSLPSYPRRR 130 140 150 160 170 180 ANATLLLGPL RASDSGLYRC QVVRGIEDEQ DLVPLEVTGV VFHYRSARDR YALTFAEAQE 190 200 210 220 230 240 ACRLSSAIIA APRHLQAAFE DGFDNCDAGW LSDRTVRYPI TQSRPGCYGD RSSLPGVRSY 250 260 270 280 290 300 GRRNPQELYD VYCFARELGG EVFYVGPARR LTLAGARAQC RRQGAALASV GQLHLAWHEG 310 320 330 340 350 360 LDQCDPGWLA DGSVRYPIQT PRRRCGGPAP GVRTVYRFAN RTGFPSPAER FDAYCFRAHH 370 380 390 400 410 420 PTSQHGDLET PSSGDEGEIL SAEGPPVREL EPTLEEEEVV TPDFQEPLVS SGEEETLILE 430 440 450 460 470 480 EKQESQQTLS PTPGDPMLAS WPTGEVWLST VAPSPSDMGA GTAASSHTEV APTDPMPRRR 490 500 510 520 530 540 GRFKGLNGRY FQQQEPEPGL QGGMEASAQP PTSEAAVNQM EPPLAMAVTE MLGSGQSRSP 550 560 570 580 590 600 WADLTNEVDM PGAGSAGGKS SPEPWLWPPT MVPPSISGHS RAPVLELEKA EGPSARPATP 610 620 630 640 650 660 DLFWSPLEAT VSAPSPAPWE AFPVATSPDL PMMAMLRGPK EWMLPHPTPI STEANRVEAH 670 680 690 700 710 720 GEATATAPPS PAAETKVYSL PLSLTPTGQG GEAMPTTPES PRADFRETGE TSPAQVNKAE 730 740 750 760 770 780 HSSSSPWPSV NRNVAVGFVP TETATEPTGL RGIPGSESGV FDTAESPTSG LQATVDEVQD 790 800 810 820 830 840 PWPSVYSKGL DASSPSAPLG SPGVFLVPKV TPNLEPWVAT DEGPTVNPMD STVTPAPSDA 850 860 870 880 890 900 SGIWEPGSQV FEEAESTTLS PQVALDTSIV TPLTTLEQGD KVGVPAMSTL GSSSSQPHPE 910 920 930 940 950 960 PEDQVETQGT SGASVPPHQS SPLGKPAVPP GTPTAASVGE SASVSSGEPT VPWDPSSTLL 970 980 990 1000 1010 1020 PVTLGIEDFE LEVLAGSPGV ESFWEEVASG EEPALPGTPM NAGAEEVHSD PCENNPCLHG 1030 1040 1050 1060 1070 1080 GTCNANGTMY GCSCDQGFAG ENCEIDIDDC LCSPCENGGT CIDEVNGFVC LCLPSYGGSF 1090 1100 1110 1120 1130 1140 CEKDTEGCDR GWHKFQGHCY RYFAHRRAWE DAEKDCRRRS GHLTSVHSPE EHSFINSFGH 1150 1160 1170 1180 1190 1200 ENTWIGLNDR IVERDFQWTD NTGLQFENWR ENQPDNFFAG GEDCVVMVAH ESGRWNDVPC 1210 1220 1230 1240 1250 1260 NYNLPYVCKK GTVLCGPPPA VENASLIGAR KAKYNVHATV RYQCNEGFAQ HHVATIRCRS 1270 1280 1290 1300 1310 1320 NGKWDRPQIV CTKPRRSHRM RRHHHHHQHH HQHHHHKSRK ERRKHKKHPT EDWEKDEGNF C

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the human neurocan gene, CSPG3." Prange C.K., Pennacchio L.A., Lieuallen K., Fan W., Lennon G.G. Gene 221:199-205(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-1254.
[2]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins." Halim A., Ruetschi U., Larson G., Nilsson J. J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

Neurocan

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF026547 mRNA. Translation: AAC80576.1. AC003110 Genomic DNA. Translation: AAB86655.1. AC005254 Genomic DNA. Translation: AAC25581.1.
IPI	IPI00159927.
RefSeq	NP_004377.2. NM_004386.2.
UniGene	Hs.169047.
3D structure databases
ProteinModelPortal	O14594.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000252575.
PTM databases
PhosphoSite	O14594.
Proteomic databases
PaxDb	O14594.
PRIDE	O14594.
Protocols and materials databases
DNASU	1463.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000252575; ENSP00000252575; ENSG00000130287.
GeneID	1463.
KEGG	hsa:1463.
UCSC	uc002nlz.3. human.
Organism-specific databases
CTD	1463.
GeneCards	GC19P019322.
H-InvDB	HIX0027449.
HGNC	HGNC:2465. NCAN.
HPA	HPA036814.
MIM	600826. gene.
neXtProt	NX_O14594.
PharmGKB	PA162396986.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG147231.
HOVERGEN	HBG078994.
InParanoid	O14594.
KO	K06794.
OrthoDB	EOG4PK27C.
PhylomeDB	O14594.
Enzyme and pathway databases
Pathway_Interaction_DB	syndecan_3_pathway. Syndecan-3-mediated signaling events.
Reactome	REACT_111045. Developmental Biology. REACT_111217. Metabolism. REACT_116125. Disease.
Gene expression databases
ArrayExpress	O14594.
Bgee	O14594.
CleanEx	HS_NCAN.
Genevestigator	O14594.
GermOnline	ENSG00000130287. Homo sapiens.
Family and domain databases
Gene3D	2.60.40.10. 1 hit. 3.10.100.10. 3 hits.
InterPro	IPR001304. C-type_lectin. IPR016186. C-type_lectin-like. IPR018378. C-type_lectin_CS. IPR016187. C-type_lectin_fold. IPR000742. EG-like_dom. IPR001881. EGF-like_Ca-bd. IPR013032. EGF-like_CS. IPR000152. EGF-type_Asp/Asn_hydroxyl_site. IPR018097. EGF_Ca-bd_CS. IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR013106. Ig_V-set. IPR000538. Link. IPR000436. Sushi_SCR_CCP. [Graphical view]
Pfam	PF00059. Lectin_C. 1 hit. PF00084. Sushi. 1 hit. PF07686. V-set. 1 hit. PF00193. Xlink. 2 hits. [Graphical view]
PRINTS	PR01265. LINKMODULE.
SMART	SM00032. CCP. 1 hit. SM00034. CLECT. 1 hit. SM00181. EGF. 1 hit. SM00179. EGF_CA. 1 hit. SM00409. IG. 1 hit. SM00445. LINK. 2 hits. [Graphical view]
SUPFAM	SSF56436. C-type_lectin_fold. 3 hits. SSF57535. Complement_control_module. 1 hit.
PROSITE	PS00010. ASX_HYDROXYL. 1 hit. PS00615. C_TYPE_LECTIN_1. 1 hit. PS50041. C_TYPE_LECTIN_2. 1 hit. PS00022. EGF_1. 3 hits. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 2 hits. PS01187. EGF_CA. 1 hit. PS50835. IG_LIKE. 1 hit. PS01241. LINK_1. 2 hits. PS50963. LINK_2. 2 hits. PS50923. SUSHI. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	1463.
NextBio	6009.
SOURCE	Search...

Entry information

Entry name

NCAN_HUMAN

Accession

Primary (citable) accession number: O14594
Secondary accession number(s): Q9UPK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 27, 2003
Last sequence update:	November 13, 2007
Last modified:	May 1, 2013
This is version 122 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.