O14576 (DC1I1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytoplasmic dynein 1 intermediate chain 1

Alternative name(s):
Cytoplasmic dynein intermediate chain 1
Dynein intermediate chain 1, cytosolic
Short name=DH IC-1

Gene names

Name:	DYNC1I1
Synonyms:	DNCI1, DNCIC1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	645 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. May play a role in mediating the interaction of cytoplasmic dynein with membranous organelles and kinetochores.
Subunit structure	Homodimer By similarity. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Interacts with DYNC1H1. Interacts with DYNLT1 and DYNLT3. Interacts with DCNT1 By similarity.
Subcellular location	Cytoplasm By similarity. Chromosome › centromere › kinetochore. Cytoplasm › cytoskeleton › spindle pole Ref.5.
Sequence similarities	Belongs to the dynein intermediate chain family. Contains 7 WD repeats.

Ontologies

Keywords
Biological process	Transport
Cellular component	Centromere Chromosome Cytoplasm Cytoskeleton Dynein Kinetochore Microtubule
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat WD repeat
Molecular function	Motor protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class II Traceable author statement. Source: Reactome vesicle transport along microtubule Inferred from sequence or structural similarity. Source: HGNC
Cellular_component	condensed chromosome kinetochore Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic dynein complex Inferred from sequence or structural similarity. Source: UniProtKB cytosol Traceable author statement. Source: Reactome kinetochore Inferred from direct assay Ref.5. Source: UniProtKB microtubule Inferred from electronic annotation. Source: UniProtKB-KW perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: HGNC spindle pole Inferred from direct assay Ref.5. Source: UniProtKB vesicle Inferred from direct assay PubMed 20682791. Source: UniProtKB
Molecular_function	microtubule binding Inferred from sequence or structural similarity. Source: HGNC microtubule motor activity Inferred from sequence or structural similarity. Source: HGNC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O14576-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O14576-2)

The sequence of this isoform differs from the canonical sequence as follows:
     74-90: Missing.

Isoform 3 (identifier: O14576-3)

The sequence of this isoform differs from the canonical sequence as follows:
     74-90: Missing.
     123-142: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 645	645	Cytoplasmic dynein 1 intermediate chain 1		PRO_0000114652
Regions
	Repeat	285 – 334	50	WD 1
	Repeat	338 – 378	41	WD 2
	Repeat	387 – 428	42	WD 3
	Repeat	437 – 477	41	WD 4
	Repeat	482 – 527	46	WD 5
	Repeat	530 – 570	41	WD 6
	Repeat	576 – 615	40	WD 7
	Region	1 – 123	123	Interaction with DCTN1 By similarity
Amino acid modifications
	Modified residue	635	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	74 – 90	17	Missing in isoform 2 and isoform 3.		VSP_001332
	Alternative sequence	123 – 142	20	Missing in isoform 3.		VSP_001333
	Natural variant	373	1	H → L Found in a renal cell carcinoma case; somatic mutation. Ref.7		VAR_064709
	Natural variant	582	1	N → T. Corresponds to variant rs35077523 [ dbSNP | Ensembl ].		VAR_048905

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 1999. Version 2. Checksum: 55A6FF971E632DA0	FASTA	645	72,955
	10 20 30 40 50 60 MSDKSDLKAE LERKKQRLAQ IREEKKRKEE ERKKKEADMQ QKKEPVQDDS DLDRKRRETE 70 80 90 100 110 120 ALLQSIGISP EPPLVQPLHF LTWDTCYFHY LVPTPMSPSS KSVSTPSEAG SQDSGDLGPL 130 140 150 160 170 180 TRTLQWDTDP SVLQLQSDSE LGRRLHKLGV SKVTQVDFLP REVVSYSKET QTPLATHQSE 190 200 210 220 230 240 EDEEDEEMVE SKVGQDSELE NQDKKQEVKE APPRELTEEE KQQILHSEEF LIFFDRTIRV 250 260 270 280 290 300 IERALAEDSD IFFDYSGREL EEKDGDVQAG ANLSFNRQFY DEHWSKHRVV TCMDWSLQYP 310 320 330 340 350 360 ELMVASYNNN EDAPHEPDGV ALVWNMKFKK TTPEYVFHCQ SSVMSVCFAR FHPNLVVGGT 370 380 390 400 410 420 YSGQIVLWDN RSHRRTPVQR TPLSAAAHTH PVYCVNVVGT QNAHNLITVS TDGKMCSWSL 430 440 450 460 470 480 DMLSTPQESM ELVYNKSKPV AVTGMAFPTG DVNNFVVGSE EGTVYTACRH GSKAGIGEVF 490 500 510 520 530 540 EGHQGPVTGI NCHMAVGPID FSHLFVTSSF DWTVKLWTTK HNKPLYSFED NADYVYDVMW 550 560 570 580 590 600 SPVHPALFAC VDGMGRLDLW NLNNDTEVPT ASVAIEGASA LNRVRWAQAG KEVAVGDSEG 610 620 630 640 RIWVYDVGEL AVPHNDEWTR FARTLVEIRA NRADSEEEGT VELSA
	Isoform 2 [UniParc]. Checksum: 0360B15D5295971F	FASTA	628	70,790
	10 20 30 40 50 60 MSDKSDLKAE LERKKQRLAQ IREEKKRKEE ERKKKEADMQ QKKEPVQDDS DLDRKRRETE 70 80 90 100 110 120 ALLQSIGISP EPPLVPTPMS PSSKSVSTPS EAGSQDSGDL GPLTRTLQWD TDPSVLQLQS 130 140 150 160 170 180 DSELGRRLHK LGVSKVTQVD FLPREVVSYS KETQTPLATH QSEEDEEDEE MVESKVGQDS 190 200 210 220 230 240 ELENQDKKQE VKEAPPRELT EEEKQQILHS EEFLIFFDRT IRVIERALAE DSDIFFDYSG 250 260 270 280 290 300 RELEEKDGDV QAGANLSFNR QFYDEHWSKH RVVTCMDWSL QYPELMVASY NNNEDAPHEP 310 320 330 340 350 360 DGVALVWNMK FKKTTPEYVF HCQSSVMSVC FARFHPNLVV GGTYSGQIVL WDNRSHRRTP 370 380 390 400 410 420 VQRTPLSAAA HTHPVYCVNV VGTQNAHNLI TVSTDGKMCS WSLDMLSTPQ ESMELVYNKS 430 440 450 460 470 480 KPVAVTGMAF PTGDVNNFVV GSEEGTVYTA CRHGSKAGIG EVFEGHQGPV TGINCHMAVG 490 500 510 520 530 540 PIDFSHLFVT SSFDWTVKLW TTKHNKPLYS FEDNADYVYD VMWSPVHPAL FACVDGMGRL 550 560 570 580 590 600 DLWNLNNDTE VPTASVAIEG ASALNRVRWA QAGKEVAVGD SEGRIWVYDV GELAVPHNDE 610 620 WTRFARTLVE IRANRADSEE EGTVELSA
	Isoform 3 [UniParc]. Checksum: D88D3EB3D1FC82B2	FASTA	608	68,575
	10 20 30 40 50 60 MSDKSDLKAE LERKKQRLAQ IREEKKRKEE ERKKKEADMQ QKKEPVQDDS DLDRKRRETE 70 80 90 100 110 120 ALLQSIGISP EPPLVPTPMS PSSKSVSTPS EAGSQDSGDL GPLTRRRLHK LGVSKVTQVD 130 140 150 160 170 180 FLPREVVSYS KETQTPLATH QSEEDEEDEE MVESKVGQDS ELENQDKKQE VKEAPPRELT 190 200 210 220 230 240 EEEKQQILHS EEFLIFFDRT IRVIERALAE DSDIFFDYSG RELEEKDGDV QAGANLSFNR 250 260 270 280 290 300 QFYDEHWSKH RVVTCMDWSL QYPELMVASY NNNEDAPHEP DGVALVWNMK FKKTTPEYVF 310 320 330 340 350 360 HCQSSVMSVC FARFHPNLVV GGTYSGQIVL WDNRSHRRTP VQRTPLSAAA HTHPVYCVNV 370 380 390 400 410 420 VGTQNAHNLI TVSTDGKMCS WSLDMLSTPQ ESMELVYNKS KPVAVTGMAF PTGDVNNFVV 430 440 450 460 470 480 GSEEGTVYTA CRHGSKAGIG EVFEGHQGPV TGINCHMAVG PIDFSHLFVT SSFDWTVKLW 490 500 510 520 530 540 TTKHNKPLYS FEDNADYVYD VMWSPVHPAL FACVDGMGRL DLWNLNNDTE VPTASVAIEG 550 560 570 580 590 600 ASALNRVRWA QAGKEVAVGD SEGRIWVYDV GELAVPHNDE WTRFARTLVE IRANRADSEE EGTVELSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of two cytoplasmic dynein intermediate chain genes in mouse and human." Crackower M.A., Sinasac D.S., Xia J., Motoyama J., Prochazka M., Rommens J.M., Scherer S.W., Tsui L.-C. Genomics 55:257-267(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Molecular cloning of a cytoplasmic dynein gene." Li G., Jin J., Hu S., Li W., Yuan J., Qiang B. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[4]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Dynein light intermediate chain 1 is required for progress through the spindle assembly checkpoint." Sivaram M.V., Wadzinski T.L., Redick S.D., Manna T., Doxsey S.J. EMBO J. 28:902-914(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-635, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[7]	"Exome sequencing identifies frequent mutation of the SWI/SNF complex gene PBRM1 in renal carcinoma." Varela I., Tarpey P., Raine K., Huang D., Ong C.K., Stephens P., Davies H., Jones D., Lin M.L., Teague J., Bignell G., Butler A., Cho J., Dalgliesh G.L., Galappaththige D., Greenman C., Hardy C., Jia M. , Latimer C., Lau K.W., Marshall J., McLaren S., Menzies A., Mudie L., Stebbings L., Largaespada D.A., Wessels L.F.A., Richard S., Kahnoski R.J., Anema J., Tuveson D.A., Perez-Mancera P.A., Mustonen V., Fischer A., Adams D.J., Rust A., Chan-On W., Subimerb C., Dykema K., Furge K., Campbell P.J., Teh B.T., Stratton M.R., Futreal P.A. Nature 469:539-542(2011) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT LEU-373.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF063228 mRNA. Translation: AAC33443.1. AF123074 mRNA. Translation: AAD26852.1. AK091339 mRNA. Translation: BAC03639.1. AC022261 Genomic DNA. No translation available. AC091779 Genomic DNA. No translation available. AC002452 Genomic DNA. Translation: AAB67047.2. AC002540 Genomic DNA. Translation: AAB70113.1.
IPI	IPI00022461. IPI00220137. IPI01012006.
RefSeq	NP_001129028.1. NM_001135556.1. NP_001129029.1. NM_001135557.1. NP_004402.1. NM_004411.4.
UniGene	Hs.440364.
3D structure databases
DisProt	DP00360.
ProteinModelPortal	O14576.
ModBase	Search...
Protein-protein interaction databases
IntAct	O14576. 3 interactions.
MINT	MINT-156403.
STRING	9606.ENSP00000320130.
PTM databases
PhosphoSite	O14576.
Proteomic databases
PaxDb	O14576.
PRIDE	O14576.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000324972; ENSP00000320130; ENSG00000158560. ENST00000359388; ENSP00000352348; ENSG00000158560. ENST00000447467; ENSP00000392337; ENSG00000158560. ENST00000457059; ENSP00000412444; ENSG00000158560.
GeneID	1780.
KEGG	hsa:1780.
UCSC	uc003uob.3. human. uc003uoc.4. human. uc003uod.4. human.
Organism-specific databases
CTD	1780.
GeneCards	GC07P095401.
HGNC	HGNC:2963. DYNC1I1.
HPA	HPA021315.
MIM	603772. gene.
neXtProt	NX_O14576.
PharmGKB	PA27434.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG308180.
HOGENOM	HOG000116383.
HOVERGEN	HBG004083.
InParanoid	O14576.
KO	K10415.
OMA	SLHFLTW.
OrthoDB	EOG4RBQJ5.
PhylomeDB	O14576.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
Gene expression databases
ArrayExpress	O14576.
Bgee	O14576.
CleanEx	HS_DYNC1I1.
Genevestigator	O14576.
GermOnline	ENSG00000158560. Homo sapiens.
Family and domain databases
Gene3D	2.130.10.10. 1 hit.
InterPro	IPR025956. Dynein_IC_1/2. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF11540. Dynein_IC2. 1 hit. PF00400. WD40. 1 hit. [Graphical view]
SMART	SM00320. WD40. 5 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. False negative. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	1780.
NextBio	7243.
SOURCE	Search...

Entry information

Entry name

DC1I1_HUMAN

Accession

Primary (citable) accession number: O14576
Secondary accession number(s): Q9Y2X1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.