O14561 (ACPM_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acyl carrier protein, mitochondrial
Short name=ACP

Alternative name(s):
CI-SDAP
NADH-ubiquinone oxidoreductase 9.6 kDa subunit

Gene names

Name:

NDUFAB1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Carrier of the growing fatty acid chain in fatty acid biosynthesis in mitochondria. Accessory and non-catalytic subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain By similarity.
Subunit structure	Mammalian complex I is composed of 45 different subunits.
Subcellular location	Mitochondrion.
Sequence similarities	Contains 1 acyl carrier domain.
Sequence caution	The sequence AAC05814.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Electron transport Fatty acid biosynthesis Lipid synthesis Respiratory chain Transport
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Phosphopantetheine
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Non-traceable author statement. Source: UniProtKB mitochondrial electron transport, NADH to ubiquinone Non-traceable author statement Ref.1. Source: UniProtKB
Cellular component	mitochondrial matrix Inferred from sequence or structural similarity. Source: UniProtKB mitochondrial respiratory chain complex I Inferred from direct assay Ref.7. Source: UniProtKB
Molecular function	NADH dehydrogenase (ubiquinone) activity Non-traceable author statement Ref.1. Source: UniProtKB acyl carrier activity Non-traceable author statement. Source: UniProtKB calcium ion binding Non-traceable author statement. Source: UniProtKB cofactor binding Inferred from electronic annotation. Source: InterPro fatty acid binding Inferred from sequence or structural similarity. Source: UniProtKB phosphopantetheine binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 68

68

Mitochondrion Ref.6

Chain

69 – 156

88

Acyl carrier protein, mitochondrial

PRO_0000000561

Amino acid modifications

Modified residue

92

1

N6-acetyllysine Ref.8

Modified residue

112

1

O-(pantetheine 4'-phosphoryl)serine Potential

Experimental info

Sequence conflict

132

1

D → Y in AAH58920. Ref.5

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

156

Helix Strand Turn

Details...

Helix

76 – 89

14

Turn

95 – 97

3

Helix

104 – 108

5

Helix

112 – 125

14

Helix

132 – 135

4

Helix

141 – 151

11

Sequences

	Sequence		Length	Mass (Da)
	O14561 [UniParc]. Last modified July 11, 2002. Version 3. Checksum: 0FCE3C3EC6D97AE2	FASTA	156	17,417
	10 20 30 40 50 60 MASRVLSAYV SRLPAAFAPL PRVRMLAVAR PLSTALCSAG TQTRLGTLQP ALVLAQVPGR 70 80 90 100 110 120 VTQLCRQYSD MPPLTLEGIQ DRVLYVLKLY DKIDPEKLSV NSHFMKDLGL DSLDQVEIIM 130 140 150 AMEDEFGFEI PDIDAEKLMC PQEIVDYIAD KKDVYE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA of eight nuclear encoded subunits of NADH:ubiquinone oxidoreductase: human complex I cDNA characterization completed." Loeffen J.L.C.M., Triepels R.H., van den Heuvel L.P., Schuelke M., Buskens C.A.F., Smeets R.J.P., Trijbels J.M.F., Smeitink J.A.M. Biochem. Biophys. Res. Commun. 253:415-422(1998) [PubMed: 9878551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q." Loftus B.J., Kim U.-J., Sneddon V.P., Kalush F., Brandon R., Fuhrmann J., Mason T., Crosby M.L., Barnstead M., Cronin L., Mays A.D., Cao Y., Xu R.X., Kang H.-L., Mitchell S., Eichler E.E., Harris P.C., Venter J.C., Adams M.D. Genomics 60:295-308(1999) [PubMed: 10493829] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[6]	"Global profiling of protease cleavage sites by chemoselective labeling of protein N-termini." Xu G., Shin S.B., Jaffrey S.R. Proc. Natl. Acad. Sci. U.S.A. 106:19310-19315(2009) [PubMed: 19892738] [Abstract] Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 69-83. Tissue: Leukemic T-cell.
[7]	"The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification." Murray J., Zhang B., Taylor S.W., Oglesbee D., Fahy E., Marusich M.F., Ghosh S.S., Capaldi R.A. J. Biol. Chem. 278:13619-13622(2003) [PubMed: 12611891] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE NADH-UBIQUINONE OXIDOREDUCTASE COMPLEX.
[8]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-92, MASS SPECTROMETRY.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]	"Solution structure of RSGI RUH-059, an ACP domain of acyl carrier protein, mitochondrial from human." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 69-156.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF087660 mRNA. Translation: AAD23566.1.
AK311877 mRNA. Translation: BAG34818.1.
AC002400 Genomic DNA. Translation: AAC05814.1. Different initiation.
CH471145 Genomic DNA. Translation: EAW55816.1.
BC058920 mRNA. Translation: AAH58920.1.

IPI

IPI00022442.

PIR

T00741.

RefSeq

NP_004994.1. NM_005003.2.

UniGene

Hs.189716.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DNW	NMR	-	A	71-156	[»]

ProteinModelPortal

O14561.

SMR

O14561. Positions 69-156.

ModBase

Search...

Protein-protein interaction databases

IntAct

O14561. 4 interactions.

STRING

O14561.

PTM databases

PhosphoSite

O14561.

Proteomic databases

PeptideAtlas

O14561.

PRIDE

O14561.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000007516; ENSP00000007516; ENSG00000004779.

GeneID

4706.

KEGG

hsa:4706.

NMPDR

fig|9606.3.peg.11884.

UCSC

uc002dlw.1. human.

Organism-specific databases

CTD

4706.

GeneCards

GC16M023592.

H-InvDB

HIX0202321.

HGNC

HGNC:7694. NDUFAB1.

MIM

603836. gene.

neXtProt

NX_O14561.

PharmGKB

PA31500.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG08677.

GeneTree

ENSGT00390000002127.

HOGENOM

HBG755218.

HOVERGEN

HBG024318.

InParanoid

O14561.

OMA

RVLCACV.

OrthoDB

EOG41VK49.

PhylomeDB

O14561.

Enzyme and pathway databases

Reactome

REACT_111217. Metabolism.

Gene expression databases

ArrayExpress

O14561.

Bgee

O14561.

CleanEx

HS_NDUFAB1.

Genevestigator

O14561.

GermOnline

ENSG00000004779. Homo sapiens.

Family and domain databases

InterPro

IPR003231. Acyl_carrier.
IPR009081. Acyl_carrier_prot-like.
IPR006163. Phsphopanteth-bd.
IPR006162. PPantetheine_attach_site.
[Graphical view]

Gene3D

G3DSA:1.10.1200.10. ACP_like. 1 hit.

KO

K03955.

Pfam

PF00550. PP-binding. 1 hit.
[Graphical view]

ProDom

PD000887. PD000887. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF47336. ACP_like. 1 hit.

TIGRFAMs

TIGR00517. Acyl_carrier. 1 hit.

PROSITE

PS50075. ACP_DOMAIN. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

DrugBank

DB00157. NADH.

NextBio

18148.

SOURCE

Search...

Entry information

Entry name

ACPM_HUMAN

Accession

Primary (citable) accession number: O14561
Secondary accession number(s): B2R4M1, Q9UNV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	July 11, 2002
Last modified:	January 25, 2012
This is version 117 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.