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O14559 (RHG33_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rho GTPase-activating protein 33
Alternative name(s):
Rho-type GTPase-activating protein 33
Sorting nexin-26
Tc10/CDC42 GTPase-activating protein
Gene names
Name:ARHGAP33
Synonyms:SNX26, TCGAP
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1287 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in several stages of intracellular trafficking. Could play an important role in the regulation of glucose transport by insulin. May act as a downstream effector of RHOQ/TC10 in the regulation of insulin-stimulated glucose transport By similarity.

Subunit structure

Specifically interacts with CDC42 and RHOQ/TC10 through its Rho-GAP domain By similarity. Interacts with NEK6. Ref.6

Sequence similarities

Belongs to the PX domain-containing GAP family.

Contains 1 PX (phox homology) domain.

Contains 1 Rho-GAP domain.

Contains 1 SH3 domain.

Sequence caution

The sequence AAB81198.1 differs from that shown. Reason: Erroneous gene model prediction.

Isoform 2: The sequence BAC86902.1 differs from that shown. Reason: Frameshift at several positions.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

FYNP062412EBI-1210010,EBI-515315

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O14559-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform 2 (identifier: O14559-10)

The sequence of this isoform differs from the canonical sequence as follows:
     1-136: Missing.
     998-1025: Missing.
Isoform 3 (identifier: O14559-11)

The sequence of this isoform differs from the canonical sequence as follows:
     648-808: Missing.
Isoform 4 (identifier: O14559-12)

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: MV → MLSLSLCSHLWGPLILSALQ
     168-228: LDNHGRRLLL...PPTEDRSWWR → VGLGRGLGDS...SLWVGESWDM
     229-1287: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12871287Rho GTPase-activating protein 33
PRO_0000056721

Regions

Domain59 – 168110PX; atypical
Domain186 – 24863SH3
Domain315 – 510196Rho-GAP
Compositional bias126 – 1294Poly-Pro
Compositional bias677 – 69923Poly-Ser
Compositional bias874 – 8796Poly-Pro
Compositional bias889 – 8935Poly-Pro
Compositional bias1006 – 10094Poly-Gly
Compositional bias1183 – 11886Poly-Ser
Compositional bias1266 – 12738Poly-Pro

Amino acid modifications

Modified residue5701Phosphoserine By similarity
Modified residue6361Phosphoserine Ref.7
Modified residue11691Phosphotyrosine By similarity

Natural variations

Alternative sequence1 – 136136Missing in isoform 2.
VSP_014287
Alternative sequence1 – 22MV → MLSLSLCSHLWGPLILSALQ in isoform 4.
VSP_014288
Alternative sequence168 – 22861LDNHG…RSWWR → VGLGRGLGDSEWVRGCVCHH AQHREILDGNRVASAVEDEG AEVDGEAFRWGSLWVGESWD M in isoform 4.
VSP_014289
Alternative sequence229 – 12871059Missing in isoform 4.
VSP_014290
Alternative sequence648 – 808161Missing in isoform 3.
VSP_014291
Alternative sequence998 – 102528Missing in isoform 2.
VSP_014292

Experimental info

Sequence conflict1191F → S in AK096338. Ref.2
Sequence conflict11001P → L in CAB70821. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2005. Version 2.
Checksum: 802900F18B640DC0

FASTA1,287137,213
        10         20         30         40         50         60 
MVARSTDSLD GPGEGSVQPL PTAGGPSVKG KPGKRLSAPR GPFPRLADCA HFHYENVDFG 

        70         80         90        100        110        120 
HIQLLLSPDR EGPSLSGENE LVFGVQVTCQ GRSWPVLRSY DDFRSLDAHL HRCIFDRRFS 

       130        140        150        160        170        180 
CLPELPPPPE GARAAQMLVP LLLQYLETLS GLVDSNLNCG PVLTWMELDN HGRRLLLSEE 

       190        200        210        220        230        240 
ASLNIPAVAA AHVIKRYTAQ APDELSFEVG DIVSVIDMPP TEDRSWWRGK RGFQVGFFPS 

       250        260        270        280        290        300 
ECVELFTERP GPGLKADADG PPCGIPAPQG ISSLTSAVPR PRGKLAGLLR TFMRSRPSRQ 

       310        320        330        340        350        360 
RLRQRGILRQ RVFGCDLGEH LSNSGQDVPQ VLRCCSEFIE AHGVVDGIYR LSGVSSNIQR 

       370        380        390        400        410        420 
LRHEFDSERI PELSGPAFLQ DIHSVSSLCK LYFRELPNPL LTYQLYGKFS EAMSVPGEEE 

       430        440        450        460        470        480 
RLVRVHDVIQ QLPPPHYRTL EYLLRHLARM ARHSANTSMH ARNLAIVWAP NLLRSMELES 

       490        500        510        520        530        540 
VGMGGAAAFR EVRVQSVVVE FLLTHVDVLF SDTFTSAGLD PAGRCLLPRP KSLAGSCPST 

       550        560        570        580        590        600 
RLLTLEEAQA RTQGRLGTPT EPTTPKAPAS PAERRKGERG EKQRKPGGSS WKTFFALGRG 

       610        620        630        640        650        660 
PSVPRKKPLP WLGGTRAPPQ PSGSRPDTVT LRSAKSEESL SSQASGAGLQ RLHRLRRPHS 

       670        680        690        700        710        720 
SSDAFPVGPA PAGSCESLSS SSSSESSSSE SSSSSSESSA AGLGALSGSP SHRTSAWLDD 

       730        740        750        760        770        780 
GDELDFSPPR CLEGLRGLDF DPLTFRCSSP TPGDPAPPAS PAPPAPASAF PPRVTPQAIS 

       790        800        810        820        830        840 
PRGPTSPASP AALDISEPLA VSVPPAVLEL LGAGGAPASA TPTPALSPGR SLRPHLIPLL 

       850        860        870        880        890        900 
LRGAEAPLTD ACQQEMCSKL RGAQGPLGPD MESPLPPPPL SLLRPGGAPP PPPKNPARLM 

       910        920        930        940        950        960 
ALALAERAQQ VAEQQSQQEC GGTPPASQSP FHRSLSLEVG GEPLGTSGSG PPPNSLAHPG 

       970        980        990       1000       1010       1020 
AWVPGPPPYL PRQQSDGSLL RSQRPMGTSR RGLRGPAQVS AQLRAGGGGR DAPEAAAQSP 

      1030       1040       1050       1060       1070       1080 
CSVPSQVPTP GFFSPAPREC LPPFLGVPKP GLYPLGPPSF QPSSPAPVWR SSLGPPAPLD 

      1090       1100       1110       1120       1130       1140 
RGENLYYEIG ASEGSPYSGP TRSWSPFRSM PPDRLNASYG MLGQSPPLHR SPDFLLSYPP 

      1150       1160       1170       1180       1190       1200 
APSCFPPDHL GYSAPQHPAR RPTPPEPLYV NLALGPRGPS PASSSSSSPP AHPRSRSDPG 

      1210       1220       1230       1240       1250       1260 
PPVPRLPQKQ RAPWGPRTPH RVPGPWGPPE PLLLYRAAPP AYGRGGELHR GSLYRNGGQR 

      1270       1280 
GEGAGPPPPY PTPSWSLHSE GQTRSYC 

« Hide

Isoform 2 [UniParc].

Checksum: 995F8936B26AF59F
Show »

FASTA1,123119,780
Isoform 3 [UniParc].

Checksum: 53E7DA764B6C1919
Show »

FASTA1,126121,066
Isoform 4 [UniParc].

Checksum: 88093F56B6055CEA
Show »

FASTA24626,858

References

« Hide 'large scale' references
[1]"A novel member (SNX26) of the sorting nexin family."
Hong W.
Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Tissue: Hippocampus.
[3]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 426-1287.
Tissue: Testis.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 993-1287.
Tissue: Pancreas.
[6]"Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome."
Vaz Meirelles G., Ferreira Lanza D.C., da Silva J.C., Santana Bernachi J., Paes Leme A.F., Kobarg J.
J. Proteome Res. 9:6298-6316(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NEK6.
[7]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-636, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY044864 mRNA. Translation: AAK97795.1.
AK096338 mRNA. No translation available.
AK127255 mRNA. Translation: BAC86902.1. Frameshift.
AC002398 Genomic DNA. Translation: AAB81197.1.
AC002398 Genomic DNA. Translation: AAB81198.1. Sequence problems.
AL137579 mRNA. Translation: CAB70821.1.
BC014084 mRNA. Translation: AAH14084.2.
PIRT00704.
T00705.
T46289.
RefSeqNP_001166101.1. NM_001172630.1.
NP_443180.2. NM_052948.3.
XP_005258545.1. XM_005258488.1.
UniGeneHs.515364.

3D structure databases

ProteinModelPortalO14559.
SMRO14559. Positions 61-249, 311-511.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid125448. 2 interactions.
IntActO14559. 2 interactions.

PTM databases

PhosphoSiteO14559.

Proteomic databases

PaxDbO14559.
PRIDEO14559.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000007510; ENSP00000007510; ENSG00000004777. [O14559-1]
ENST00000314737; ENSP00000320038; ENSG00000004777. [O14559-11]
ENST00000378944; ENSP00000368227; ENSG00000004777. [O14559-10]
GeneID115703.
KEGGhsa:115703.
UCSCuc002obr.2. human. [O14559-1]
uc002obs.2. human. [O14559-11]
uc002obt.2. human. [O14559-10]
uc010eek.2. human. [O14559-12]

Organism-specific databases

CTD115703.
GeneCardsGC19P036266.
HGNCHGNC:23085. ARHGAP33.
HPAHPA030117.
HPA030118.
MIM614902. gene.
neXtProtNX_O14559.
PharmGKBPA165393003.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG311367.
HOGENOMHOG000154522.
HOVERGENHBG079688.
InParanoidO14559.
KOK17933.
OMACFPPDHL.
OrthoDBEOG7WMCHV.
PhylomeDBO14559.
TreeFamTF351451.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressO14559.
BgeeO14559.
CleanExHS_SNX26.
GenevestigatorO14559.

Family and domain databases

Gene3D1.10.555.10. 1 hit.
InterProIPR001683. Phox.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamPF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTSM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEPS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiSNX26.
GenomeRNAi115703.
NextBio79650.
PROO14559.
SOURCESearch...

Entry information

Entry nameRHG33_HUMAN
AccessionPrimary (citable) accession number: O14559
Secondary accession number(s): O14552 expand/collapse secondary AC list , O14560, Q6ZSP6, Q96CP3, Q9NT23
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: July 5, 2005
Last modified: April 16, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM