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O14556

- G3PT_HUMAN

UniProt

O14556 - G3PT_HUMAN

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Protein

Glyceraldehyde-3-phosphate dehydrogenase, testis-specific

Gene
GAPDHS, GAPD2, GAPDH2, GAPDS, HSD-35, HSD35
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility By similarity.

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei106 – 1061NAD
Binding sitei151 – 1511NAD; via carbonyl oxygen
Binding sitei173 – 1731NAD
Binding sitei193 – 1931NAD
Active sitei224 – 2241Nucleophile1 Publication
Sitei251 – 2511Activates thiol group during catalysis
Binding sitei254 – 2541Glyceraldehyde 3-phosphate By similarity
Binding sitei306 – 3061Glyceraldehyde 3-phosphate By similarity
Binding sitei388 – 3881NAD

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi85 – 862NAD

GO - Molecular functioni

  1. glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Source: UniProtKB
  2. NAD binding Source: InterPro
  3. NADP binding Source: InterPro
  4. protein binding Source: UniProtKB

GO - Biological processi

  1. carbohydrate metabolic process Source: Reactome
  2. gluconeogenesis Source: Reactome
  3. glucose metabolic process Source: Reactome
  4. glycolytic process Source: Reactome
  5. positive regulation of glycolytic process Source: UniProtKB
  6. small molecule metabolic process Source: Reactome
  7. spermatid development Source: Ensembl
  8. sperm motility Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMetaCyc:HS02793-MONOMER.
ReactomeiREACT_1383. Glycolysis.
REACT_1520. Gluconeogenesis.
UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (EC:1.2.1.12)
Alternative name(s):
Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2
Short name:
GAPDH-2
Spermatogenic glyceraldehyde-3-phosphate dehydrogenase
Gene namesi
Name:GAPDHS
Synonyms:GAPD2, GAPDH2, GAPDS
ORF Names:HSD-35, HSD35
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:24864. GAPDHS.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. motile cilium Source: Ensembl
  3. nucleus Source: UniProt
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134934259.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 408408Glyceraldehyde-3-phosphate dehydrogenase, testis-specificPRO_0000145502Add
BLAST

Proteomic databases

MaxQBiO14556.
PaxDbiO14556.
PeptideAtlasiO14556.
PRIDEiO14556.

PTM databases

PhosphoSiteiO14556.

Expressioni

Tissue specificityi

Testis specific.

Gene expression databases

ArrayExpressiO14556.
BgeeiO14556.
CleanExiHS_GAPDHS.
GenevestigatoriO14556.

Organism-specific databases

HPAiHPA042666.

Interactioni

Subunit structurei

Homotetramer. Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation.3 Publications

Protein-protein interaction databases

BioGridi117681. 3 interactions.
IntActiO14556. 2 interactions.
MINTiMINT-1515577.
STRINGi9606.ENSP00000222286.

Structurei

Secondary structure

1
408
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi77 – 815
Helixi85 – 9612
Beta strandi100 – 1056
Helixi111 – 1199
Turni122 – 1243
Beta strandi131 – 1344
Beta strandi137 – 1404
Beta strandi143 – 1486
Helixi153 – 1553
Helixi158 – 1614
Beta strandi165 – 1684
Beta strandi170 – 1723
Helixi176 – 1849
Beta strandi188 – 1947
Beta strandi197 – 1993
Turni204 – 2063
Helixi208 – 2103
Turni213 – 2153
Beta strandi217 – 2204
Helixi224 – 24017
Beta strandi242 – 25211
Beta strandi257 – 2615
Helixi268 – 2714
Turni274 – 2763
Beta strandi279 – 2813
Helixi285 – 2928
Helixi294 – 2963
Turni297 – 2993
Beta strandi300 – 3089
Beta strandi313 – 32311
Helixi327 – 33913
Turni340 – 3456
Beta strandi346 – 3494
Helixi355 – 3584
Beta strandi364 – 3685
Turni369 – 3713
Beta strandi373 – 3764
Beta strandi379 – 3868
Helixi390 – 40718

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H9EX-ray1.72O/P69-407[»]
3PFWX-ray2.15O/P69-407[»]
ProteinModelPortaliO14556.
SMRiO14556. Positions 74-407.

Miscellaneous databases

EvolutionaryTraceiO14556.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 7373Testis-specific N-terminal extensionAdd
BLAST
Regioni223 – 2253Glyceraldehyde 3-phosphate binding By similarity
Regioni283 – 2842Glyceraldehyde 3-phosphate binding By similarity

Domaini

The testis-specific N-terminal extension mediates tight association with the cytoskeletal fibrous sheath of the spermatozoa flagellum, possibly via interchain disulfide-bonding of Cys-21 with sheath components (1 Publication).1 Publication

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0057.
HOGENOMiHOG000071678.
HOVERGENiHBG000227.
InParanoidiO14556.
KOiK10705.
OMAiNDKWGIE.
OrthoDBiEOG7Q5HDF.
PhylomeDBiO14556.
TreeFamiTF300533.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O14556-1 [UniParc]FASTAAdd to Basket

« Hide

MSKRDIVLTN VTVVQLLRQP CPVTRAPPPP EPKAEVEPQP QPEPTPVREE    50
IKPPPPPLPP HPATPPPKMV SVARELTVGI NGFGRIGRLV LRACMEKGVK 100
VVAVNDPFID PEYMVYMFKY DSTHGRYKGS VEFRNGQLVV DNHEISVYQC 150
KEPKQIPWRA VGSPYVVEST GVYLSIQAAS DHISAGAQRV VISAPSPDAP 200
MFVMGVNEND YNPGSMNIVS NASCTTNCLA PLAKVIHERF GIVEGLMTTV 250
HSYTATQKTV DGPSRKAWRD GRGAHQNIIP ASTGAAKAVT KVIPELKGKL 300
TGMAFRVPTP DVSVVDLTCR LAQPAPYSAI KEAVKAAAKG PMAGILAYTE 350
DEVVSTDFLG DTHSSIFDAK AGIALNDNFV KLISWYDNEY GYSHRVVDLL 400
RYMFSRDK 408
Length:408
Mass (Da):44,501
Last modified:May 30, 2000 - v2
Checksum:i301F71C768CD95D8
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti110 – 1101D → N.
Corresponds to variant rs2285514 [ dbSNP | Ensembl ].
VAR_049219

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti220 – 2201S → SVRAHLGCFS in AAB64181. 1 Publication
Sequence conflicti273 – 2731G → V in AAQ75383. 1 Publication
Sequence conflicti343 – 3431A → R in AAF87970. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005371 mRNA. Translation: CAA06501.1.
AF216641
, AF216631, AF216632, AF216633, AF216634, AF216635, AF216636, AF216637, AF216638, AF216639, AF216640 Genomic DNA. Translation: AAF87970.1.
AY306129 mRNA. Translation: AAQ75383.1.
AK314980 mRNA. Translation: BAG37479.1.
AC002389 Genomic DNA. Translation: AAB64181.1.
BC036373 mRNA. Translation: AAH36373.1.
CCDSiCCDS12465.1.
RefSeqiNP_055179.1. NM_014364.4.
UniGeneiHs.248017.

Genome annotation databases

EnsembliENST00000222286; ENSP00000222286; ENSG00000105679.
GeneIDi26330.
KEGGihsa:26330.
UCSCiuc002oaf.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005371 mRNA. Translation: CAA06501.1 .
AF216641
, AF216631 , AF216632 , AF216633 , AF216634 , AF216635 , AF216636 , AF216637 , AF216638 , AF216639 , AF216640 Genomic DNA. Translation: AAF87970.1 .
AY306129 mRNA. Translation: AAQ75383.1 .
AK314980 mRNA. Translation: BAG37479.1 .
AC002389 Genomic DNA. Translation: AAB64181.1 .
BC036373 mRNA. Translation: AAH36373.1 .
CCDSi CCDS12465.1.
RefSeqi NP_055179.1. NM_014364.4.
UniGenei Hs.248017.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3H9E X-ray 1.72 O/P 69-407 [» ]
3PFW X-ray 2.15 O/P 69-407 [» ]
ProteinModelPortali O14556.
SMRi O14556. Positions 74-407.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 117681. 3 interactions.
IntActi O14556. 2 interactions.
MINTi MINT-1515577.
STRINGi 9606.ENSP00000222286.

Chemistry

DrugBanki DB00157. NADH.

PTM databases

PhosphoSitei O14556.

Proteomic databases

MaxQBi O14556.
PaxDbi O14556.
PeptideAtlasi O14556.
PRIDEi O14556.

Protocols and materials databases

DNASUi 26330.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000222286 ; ENSP00000222286 ; ENSG00000105679 .
GeneIDi 26330.
KEGGi hsa:26330.
UCSCi uc002oaf.1. human.

Organism-specific databases

CTDi 26330.
GeneCardsi GC19P036024.
HGNCi HGNC:24864. GAPDHS.
HPAi HPA042666.
MIMi 609169. gene.
neXtProti NX_O14556.
PharmGKBi PA134934259.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0057.
HOGENOMi HOG000071678.
HOVERGENi HBG000227.
InParanoidi O14556.
KOi K10705.
OMAi NDKWGIE.
OrthoDBi EOG7Q5HDF.
PhylomeDBi O14556.
TreeFami TF300533.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00184 .
BioCyci MetaCyc:HS02793-MONOMER.
Reactomei REACT_1383. Glycolysis.
REACT_1520. Gluconeogenesis.

Miscellaneous databases

EvolutionaryTracei O14556.
GeneWikii GAPDHS.
GenomeRNAii 26330.
NextBioi 48663.
PROi O14556.
SOURCEi Search...

Gene expression databases

ArrayExpressi O14556.
Bgeei O14556.
CleanExi HS_GAPDHS.
Genevestigatori O14556.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR10836. PTHR10836. 1 hit.
Pfami PF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000149. GAP_DH. 1 hit.
PRINTSi PR00078. G3PDHDRGNASE.
SMARTi SM00846. Gp_dh_N. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01534. GAPDH-I. 1 hit.
PROSITEi PS00071. GAPDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of human testis-specific glyceraldehyde-3-phosphate dehydrogenase (GAPDH-2) cDNA."
    McLaughlin E.A., Hall L.
    Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  2. "Human glyceraldehyde 3-phosphate dehydrogenase-2 gene is expressed specifically in spermatogenic cells."
    Welch J.E., Brown P.L., O'Brien D.A., Magyar P.L., Bunch D.O., Mori C., Eddy E.M.
    J. Androl. 21:328-338(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "A new spermatogenesis-related gene."
    Zhao H., Miao S.Y., Zhang X.D., Liang G., Qiao Y., Wang L.F.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  5. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  7. "Novel function of beta-arrestin2 in the nucleus of mature spermatozoa."
    Neuhaus E.M., Mashukova A., Barbour J., Wolters D., Hatt H.
    J. Cell Sci. 119:3047-3056(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ARRB2.
  8. "Investigation of glyceraldehyde-3-phosphate dehydrogenase from human sperms."
    Shchutskaya Y.Y., Elkina Y.L., Kuravsky M.L., Bragina E.E., Schmalhausen E.V.
    Biochemistry (Mosc.) 73:185-191(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN N-TERMINAL EXTENSION, SUBUNIT.
  9. "Structure and kinetic characterization of human sperm-specific glyceraldehyde-3-phosphate dehydrogenase, GAPDS."
    Chaikuad A., Shafqat N., Al-Mokhtar R., Cameron G., Clarke A.R., Brady R.L., Oppermann U., Frayne J., Yue W.W.
    Biochem. J. 435:401-409(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.72 ANGSTROMS) OF 69-407 IN COMPLEX WITH NAD AND GLYCEROL, ACTIVE SITE, SUBUNIT.

Entry informationi

Entry nameiG3PT_HUMAN
AccessioniPrimary (citable) accession number: O14556
Secondary accession number(s): B2RC82
, O60823, Q6JTT9, Q9HCU6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: September 3, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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