##gff-version 3
O14543	UniProtKB	Chain	1	225	.	.	.	ID=PRO_0000181243;Note=Suppressor of cytokine signaling 3	
O14543	UniProtKB	Domain	46	142	.	.	.	Note=SH2;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00191	
O14543	UniProtKB	Domain	177	224	.	.	.	Note=SOCS box;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00194	
O14543	UniProtKB	Region	22	33	.	.	.	Note=Kinase inhibitory region (KIR)	
O14543	UniProtKB	Region	34	45	.	.	.	Note=Extended SH2 subdomain (ESS)	
O14543	UniProtKB	Region	131	162	.	.	.	Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
O14543	UniProtKB	Compositional bias	131	146	.	.	.	Note=Pro residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
O14543	UniProtKB	Natural variant	125	125	.	.	.	ID=VAR_030033;Note=H->Y;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9266833;Dbxref=dbSNP:rs1061489,PMID:9266833	
O14543	UniProtKB	Mutagenesis	22	22	.	.	.	Note=Little effect on EPO-induced STAT5 signaling suppression. L->A%2CF;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10421843;Dbxref=PMID:10421843	
O14543	UniProtKB	Mutagenesis	22	22	.	.	.	Note=Complete loss of EPO-induced STAT5 signaling suppression. No suppression of JAK2 phosphorylation. L->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10421843;Dbxref=PMID:10421843	
O14543	UniProtKB	Mutagenesis	25	25	.	.	.	Note=Complete loss of EPO-induced STAT5 signaling suppression. Abolishes binding to JH1. F->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10421843;Dbxref=PMID:10421843	
O14543	UniProtKB	Mutagenesis	30	30	.	.	.	Note=Partial loss of EPO-induced STAT5 signaling suppression. No effect on LIF-induced signaling suppression. Abolishes binding to JH1. Inhibits JAK2 phosphorylation. E->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10421843;Dbxref=PMID:10421843	
O14543	UniProtKB	Mutagenesis	31	31	.	.	.	Note=Complete loss of EPO-induced STAT5 signaling suppression. No effect on LIF-induced STAT3 signaling. Abolishes binding to JH1. Y->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10421843;Dbxref=PMID:10421843	
O14543	UniProtKB	Mutagenesis	31	31	.	.	.	Note=Little effect on EPO-induced signaling suppression. Y->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10421843;Dbxref=PMID:10421843	
O14543	UniProtKB	Mutagenesis	34	34	.	.	.	Note=Complete loss of EPO/LIF-induced signaling suppression. V->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10421843;Dbxref=PMID:10421843	
O14543	UniProtKB	Mutagenesis	41	41	.	.	.	Note=Complete loss of EPO/LIF-induced signaling inhibition. Abolishes binding to JH1. L->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10421843;Dbxref=PMID:10421843	
O14543	UniProtKB	Mutagenesis	45	45	.	.	.	Note=Little effect on EPO/LIF signaling. G->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10421843;Dbxref=PMID:10421843	
O14543	UniProtKB	Mutagenesis	53	53	.	.	.	Note=No effect on binding to Y429/Y431 phosphorylated EPOR. G->V;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12027890;Dbxref=PMID:12027890	
O14543	UniProtKB	Mutagenesis	58	58	.	.	.	Note=Impaired binding to Y429/Y431 phosphorylated EPOR. L->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12027890;Dbxref=PMID:12027890	
O14543	UniProtKB	Mutagenesis	71	71	.	.	.	Note=Complete loss of EPO/LIF-induced signaling suppression. No inhibition of JAK2 phosphorylation. R->E;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:10421843,ECO:0000269|PubMed:14559241;Dbxref=PMID:10421843,PMID:14559241	
O14543	UniProtKB	Mutagenesis	71	71	.	.	.	Note=No effect on EPO/LIF-induced signaling suppression. Partial suppression of JAK2 phosphorylation. No effect on binding to JH1. Loss of binding to IL12RB2. R->K;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:10421843,ECO:0000269|PubMed:14559241;Dbxref=PMID:10421843,PMID:14559241	
O14543	UniProtKB	Mutagenesis	93	93	.	.	.	Note=Impaired binding to Y429/Y431 phosphorylated EPOR. L->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12027890;Dbxref=PMID:12027890	
O14543	UniProtKB	Mutagenesis	94	94	.	.	.	Note=Greatly impaired binding to Y429/Y431 phosphorylated EPOR. R->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12027890;Dbxref=PMID:12027890	
O14543	UniProtKB	Sequence conflict	81	81	.	.	.	Note=T->A;Ontology_term=ECO:0000305;evidence=ECO:0000305