O14529 (CUX2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homeobox protein cut-like 2

Alternative name(s):
Homeobox protein cux-2

Gene names

Name:	CUX2
Synonyms:	CUTL2, KIAA0293

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1486 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be a transcription factor involved in neural specification. Binds to DNA in a sequence-specific manner By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the CUT homeobox family. Contains 3 CUT DNA-binding domains. Contains 1 homeobox DNA-binding domain.
Sequence caution	The sequence BAA22962.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Coiled coil Homeobox Repeat
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to organic substance Inferred from electronic annotation. Source: Compara negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Non-traceable author statement. Source: UniProtKB
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1486

1486

Homeobox protein cut-like 2

PRO_0000202396

Regions

DNA binding

544 – 631

88

CUT 1

DNA binding

887 – 974

88

CUT 2

DNA binding

1038 – 1125

88

CUT 3

DNA binding

1168 – 1227

60

Homeobox

Coiled coil

195 – 374

180

Potential

Coiled coil

690 – 717

28

Potential

Compositional bias

440 – 535

96

Pro-rich

Compositional bias

802 – 813

12

Poly-Ser

Compositional bias

1327 – 1437

111

Pro-rich

Amino acid modifications

Modified residue

143

1

Phosphoserine Ref.5

Natural variations

Natural variant

1472

1

V → L. Ref.1 Ref.4
Corresponds to variant rs6490073 [ dbSNP | Ensembl ].

VAR_065096

Secondary structure

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1486

Helix Strand Turn

Details...

Helix

555 – 568

14

Helix

573 – 579

7

Helix

585 – 593

9

Helix

598 – 600

3

Helix

603 – 616

14

Helix

620 – 630

11

Helix

889 – 893

5

Helix

898 – 910

13

Turn

911 – 913

3

Helix

916 – 922

7

Helix

928 – 936

9

Turn

941 – 943

3

Helix

946 – 961

16

Helix

1037 – 1043

7

Helix

1049 – 1062

14

Helix

1067 – 1073

7

Helix

1079 – 1087

9

Turn

1092 – 1094

3

Helix

1097 – 1111

15

Helix

1115 – 1123

9

Sequences

	Sequence		Length	Mass (Da)
	O14529 [UniParc]. Last modified November 30, 2010. Version 4. Checksum: 71782EF5214D0262	FASTA	1,486	161,677
	10 20 30 40 50 60 MAANVGSMFQ YWKRFDLRRL QKELNSVASE LSARQEESEH SHKHLIELRR EFKKNVPEEI 70 80 90 100 110 120 REMVAPVLKS FQAEVVALSK RSQEAEAAFL SVYKQLIEAP DPVPVFEAAR SLDDRLQPPS 130 140 150 160 170 180 FDPSGQPRRD LHTSWKRNPE LLSPKEQREG TSPAGPTLTE GSRLPGIPGK ALLTETLLQR 190 200 210 220 230 240 NEAEKQKGLQ EVQITLAARL GEAEEKIKVL HSALKATQAE LLELRRKYDE EAASKADEVG 250 260 270 280 290 300 LIMTNLEKAN QRAEAAQREV ESLREQLASV NSSIRLACCS PQGPSGDKVN FTLCSGPRLE 310 320 330 340 350 360 AALASKDREI LRLLKDVQHL QSSLQELEEA SANQIADLER QLTAKSEAIE KLEEKLQAQS 370 380 390 400 410 420 DYEEIKTELS ILKAMKLASS TCSLPQGMAK PEDSLLIAKE AFFPTQKFLL EKPSLLASPE 430 440 450 460 470 480 EDPSEDDSIK DSLGTEQSYP SPQQLPPPPG PEDPLSPSPG QPLLGPSLGP DGTRTFSLSP 490 500 510 520 530 540 FPSLASGERL MMPPAAFKGE AGGLLVFPPA FYGAKPPTAP ATPAPGPEPL GGPEPADGGG 550 560 570 580 590 600 GGAAGPGAEE EQLDTAEIAF QVKEQLLKHN IGQRVFGHYV LGLSQGSVSE ILARPKPWRK 610 620 630 640 650 660 LTVKGKEPFI KMKQFLSDEQ NVLALRTIQV RQRGSITPRI RTPETGSDDA IKSILEQAKK 670 680 690 700 710 720 EIESQKGGEP KTSVAPLSIA NGTTPASTSE DAIKSILEQA RREMQAQQQA LLEMEVAPRG 730 740 750 760 770 780 RSVPPSPPER PSLATASQNG APALVKQEEG SGGPAQAPLP VLSPAAFVQS IIRKVKSEIG 790 800 810 820 830 840 DAGYFDHHWA SDRGLLSRPY ASVSPSLSSS SSSGYSGQPN GRAWPRGDEA PVPPEDEAAA 850 860 870 880 890 900 GAEDEPPRTG ELKAEGATAE AGARLPYYPA YVPRTLKPTV PPLTPEQYEL YMYREVDTLE 910 920 930 940 950 960 LTRQVKEKLA KNGICQRIFG EKVLGLSQGS VSDMLSRPKP WSKLTQKGRE PFIRMQLWLS 970 980 990 1000 1010 1020 DQLGQAVGQQ PGASQASPTE PRSSPSPPPS PTEPEKSSQE PLSLSLESSK ENQQPEGRSS 1030 1040 1050 1060 1070 1080 SSLSGKMYSG SQAPGGIQEI VAMSPELDTY SITKRVKEVL TDNNLGQRLF GESILGLTQG 1090 1100 1110 1120 1130 1140 SVSDLLSRPK PWHKLSLKGR EPFVRMQLWL NDPHNVEKLR DMKKLEKKAY LKRRYGLIST 1150 1160 1170 1180 1190 1200 GSDSESPATR SECPSPCLQP QDLSLLQIKK PRVVLAPEEK EALRKAYQLE PYPSQQTIEL 1210 1220 1230 1240 1250 1260 LSFQLNLKTN TVINWFHNYR SRMRREMLVE GTQDEPDLDP SGGPGILPPG HSHPDPTPQS 1270 1280 1290 1300 1310 1320 PDSETEDQKP TVKELELQEG PEENSTPLTT QDKAQVRIKQ EQMEEDAEEE AGSQPQDSGE 1330 1340 1350 1360 1370 1380 LDKGQGPPKE EHPDPPGNDG LPKVAPGPLL PGGSTPDCPS LHPQQESEAG ERLHPDPLSF 1390 1400 1410 1420 1430 1440 KSASESSRCS LEVSLNSPSA ASSPGLMMSV SPVPSSSAPI SPSPPGAPPA KVPSASPTAD 1450 1460 1470 1480 MAGALHPSAK VNPNLQRRHE KMANLNNIIY RVERAANREE ALEWEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Construction and characterization of human brain cDNA libraries suitable for analysis of cDNA clones encoding relatively large proteins." Ohara O., Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Nomura N. DNA Res. 4:53-59(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-1472. Tissue: Brain.
[2]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-1472. Tissue: Brain.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Solution structure of the first, second and third CUT domains of human homeobox protein CUX-2 (CUT-like 2)." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 544-631 AND 887-1125.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB006631 mRNA. Translation: BAA22962.2. Different initiation.
AC005805 Genomic DNA. No translation available.
AC002979 Genomic DNA. No translation available.
AC002352 Genomic DNA. No translation available.
AC002978 Genomic DNA. No translation available.
BC151245 mRNA. Translation: AAI51246.1.

IPI

IPI00022370.

RefSeq

NP_056082.2. NM_015267.3.

UniGene

Hs.124953.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WH6	NMR	-	A	887-974	[»]
1WH8	NMR	-	A	1028-1125	[»]
1X2L	NMR	-	A	544-631	[»]

ProteinModelPortal

O14529.

SMR

O14529. Positions 549-631, 887-976, 1032-1220.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000261726.

PTM databases

PhosphoSite

O14529.

Proteomic databases

PaxDb

O14529.

PRIDE

O14529.

Protocols and materials databases

DNASU

23316.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000261726; ENSP00000261726; ENSG00000111249.

GeneID

23316.

KEGG

hsa:23316.

UCSC

uc001tsa.2. human.

Organism-specific databases

CTD

23316.

GeneCards

GC12P111471.

HGNC

HGNC:19347. CUX2.

HPA

HPA044772.

MIM

610648. gene.

neXtProt

NX_O14529.

PharmGKB

PA162382977.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG325167.

HOGENOM

HOG000143386.

HOVERGEN

HBG051268.

InParanoid

O14529.

KO

K09313.

OMA

LVFPPAF.

OrthoDB

EOG4K9BBG.

Gene expression databases

ArrayExpress

O14529.

Bgee

O14529.

CleanEx

HS_CUX2.

Genevestigator

O14529.

GermOnline

ENSG00000111249. Homo sapiens.

Family and domain databases

Gene3D

1.10.10.60. 1 hit.
1.10.260.40. 3 hits.

InterPro

IPR003350. Hmoeo_CUT.
IPR017970. Homeobox_CS.
IPR001356. Homeodomain.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
[Graphical view]

Pfam

PF02376. CUT. 3 hits.
PF00046. Homeobox. 1 hit.
[Graphical view]

SMART

SM00389. HOX. 1 hit.
[Graphical view]

SUPFAM

SSF46689. Homeodomain_like. 1 hit.
SSF47413. Lambda_like_DNA. 3 hits.

PROSITE

PS51042. CUT. 3 hits.
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O14529.

GenomeRNAi

23316.

NextBio

45200.

SOURCE

Search...

Entry information

Entry name

CUX2_HUMAN

Accession

Primary (citable) accession number: O14529
Secondary accession number(s): A7E2Y4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	November 30, 2010
Last modified:	May 1, 2013
This is version 123 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.