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O14526 (FCHO1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
FCH domain only protein 1
Gene names
Name:FCHO1
Synonyms:KIAA0290
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length889 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions in an early step of clathrin-mediated endocytosis. Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. May regulate Bmp signaling by regulating clathrin-mediated endocytosis of Bmp receptors. Ref.8

Subunit structure

May oligomerize and form homotetramer By similarity. Interacts with AP2A2 and AP2B1; 2 subunits of the adaptor protein complex AP-2. Interacts with DAB2. Interacts with clathrin (CLTC or CLTCL1). Interacts with EPS15, EPS15R and ITSN1. Interacts with AGFG1 and CALM. May interact with ACVR1; linking this receptor to clathrin-mediated endocytosis. Ref.9

Subcellular location

Membraneclathrin-coated pit; Peripheral membrane protein; Cytoplasmic side Probable. Note: Associated with forming but not mature clathrin-coated vesicles. The recruitment to coated-pits precede the one of clathrin and the adaptor protein complex AP-2. According to Ref.6 it may also dynamically associate with Golgi/TGN membranes. Ref.6 Ref.8

Sequence similarities

Belongs to the FCHO family.

Contains 1 FCH domain.

Contains 1 MHD (mu homology) domain.

Sequence caution

The sequence BAA22959.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processEndocytosis
   Cellular componentCoated pit
Membrane
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processclathrin coat assembly

Inferred from mutant phenotype Ref.8. Source: UniProtKB

clathrin-mediated endocytosis

Inferred from mutant phenotype Ref.8. Source: UniProtKB

   Cellular_componentcoated pit

Inferred from direct assay Ref.8. Source: UniProtKB

plasma membrane

Inferred from direct assay Ref.8. Source: UniProtKB

   Molecular_functionAP-2 adaptor complex binding

Inferred from direct assay Ref.9. Source: MGI

protein binding

Inferred from physical interaction PubMed 19713939. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

A7BFV92EBI-719823,EBI-6095043From a different organism.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O14526-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O14526-2)

The sequence of this isoform differs from the canonical sequence as follows:
     883-889: GMYLVSC → AAPPQG
Note: No experimental confirmation available.
Isoform 3 (identifier: O14526-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-50: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 889889FCH domain only protein 1
PRO_0000271759

Regions

Domain2 – 8382FCH
Domain625 – 888264MHD
Region1 – 275275Mediates membrane-binding
Region267 – 442176Mediates interaction with the adaptor protein complex AP-2
Region609 – 889281Mediates interaction with AGFG1, CALM, DAB2, EPS15, EPS15R, ITSN1 and clathrin
Coiled coil156 – 19540 Potential
Compositional bias375 – 3784Poly-Ala
Compositional bias465 – 4684Poly-Ser
Compositional bias480 – 56182Pro-rich
Compositional bias671 – 6744Poly-Pro

Amino acid modifications

Modified residue6161Phosphoserine Ref.7

Natural variations

Alternative sequence1 – 5050Missing in isoform 3.
VSP_046906
Alternative sequence883 – 8897GMYLVSC → AAPPQG in isoform 2.
VSP_022338

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 9, 2007. Version 2.
Checksum: 8CDA1F24F42256D2

FASTA88996,861
        10         20         30         40         50         60 
MSYFGEHFWG EKNHGFEVLY HSVKQGPIST KELADFIRER ATIEETYSKA MAKLSKLASN 

        70         80         90        100        110        120 
GTPMGTFAPL WEVFRVSSDK LALCHLELTR KLQDLIKDVL RYGEEQLKTH KKCKEEVVST 

       130        140        150        160        170        180 
LDAVQVLSGV SQLLPKSREN YLNRCMDQER LRRESTSQKE MDKAETKTKK AAESLRRSVE 

       190        200        210        220        230        240 
KYNSARADFE QKMLDSALRF QAMEETHLRH MKALLGSYAH SVEDTHVQIG QVHEEFKQNI 

       250        260        270        280        290        300 
ENVSVEMLLR KFAESKGTGR EKPGPLDFEA YSAAALQEAM KRLRGAKAFR LPGLSRRERE 

       310        320        330        340        350        360 
PEPPAAVDFL EPDSGTCPEV DEEGFTVRPD VTQNSTAEPS RFSSSDSDFD DEEPRKFYVH 

       370        380        390        400        410        420 
IKPAPARAPA CSPEAAAAQL RATAGSLILP PGPGGTMKRH SSRDAAGKPQ RPRSAPRTSS 

       430        440        450        460        470        480 
CAERLQSEEQ VSKNLFGPPL ESAFDHEDFT GSSSLGFTSS PSPFSSSSPE NVEDSGLDSP 

       490        500        510        520        530        540 
SHAAPGPSPD SWVPRPGTPQ SPPSCRAPPP EARGIRAPPL PDSPQPLASS PGPWGLEALA 

       550        560        570        580        590        600 
GGDLMPAPAD PTAREGLAAP PRRLRSRKVS CPLTRSNGDL SRSLSPSPLG SSAASTALER 

       610        620        630        640        650        660 
PSFLSQTGHG VSRGPSPVVL GSQDALPIAT AFTEYVHAYF RGHSPSCLAR VTGELTMTFP 

       670        680        690        700        710        720 
AGIVRVFSGT PPPPVLSFRL VHTTAIEHFQ PNADLLFSDP SQSDPETKDF WLNMAALTEA 

       730        740        750        760        770        780 
LQRQAEQNPT ASYYNVVLLR YQFSRPGPQS VPLQLSAHWQ CGATLTQVSV EYGYRPGATA 

       790        800        810        820        830        840 
VPTPLTNVQI LLPVGEPVTN VRLQPAATWN LEEKRLTWRL PDVSEAGGSG RLSASWEPLS 

       850        860        870        880 
GPSTPSPVAA QFTSEGTTLS GVDLELVGSG YRMSLVKRRF ATGMYLVSC 

« Hide

Isoform 2 [UniParc].

Checksum: 27DB24B8191372A9
Show »

FASTA88896,629
Isoform 3 [UniParc].

Checksum: 4CC94A2046D108CE
Show »

FASTA83991,013

References

« Hide 'large scale' references
[1]"Construction and characterization of human brain cDNA libraries suitable for analysis of cDNA clones encoding relatively large proteins."
Ohara O., Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Nomura N.
DNA Res. 4:53-59(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Brain and Thymus.
[3]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Blood.
[6]"Dynamic behavior of FCHO1 revealed by live-cell imaging microscopy: its possible involvement in clathrin-coated vesicle formation."
Sakaushi S., Inoue K., Zushi H., Senda-Murata K., Fukada T., Oka S., Sugimoto K.
Biosci. Biotechnol. Biochem. 71:1764-1768(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[7]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-616, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[8]"FCHo proteins are nucleators of clathrin-mediated endocytosis."
Henne W.M., Boucrot E., Meinecke M., Evergren E., Vallis Y., Mittal R., McMahon H.T.
Science 328:1281-1284(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN CLATHRIN-COATED PITS NUCLEATION, SUBCELLULAR LOCATION.
[9]"Distinct and separable activities of the endocytic clathrin-coat components Fcho1/2 and AP-2 in developmental patterning."
Umasankar P.K., Sanker S., Thieman J.R., Chakraborty S., Wendland B., Tsang M., Traub L.M.
Nat. Cell Biol. 14:488-501(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: LIPID-BINDING, INTERACTION WITH ACVR1; AGFG1; AP2A2; AP2B1; CALM; DAB2; EPS15; EPS15R; ITSN1 AND CLATHRIN.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB006628 mRNA. Translation: BAA22959.1. Different initiation.
AK291410 mRNA. Translation: BAF84099.1.
AK303623 mRNA. Translation: BAG64632.1.
AC008761 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84629.1.
BC028021 mRNA. Translation: AAH28021.1.
BC041130 mRNA. Translation: AAH41130.1.
CCDSCCDS32955.1. [O14526-1]
CCDS59366.1. [O14526-3]
PIRT00039.
RefSeqNP_001154829.1. NM_001161357.1.
NP_001154830.1. NM_001161358.1. [O14526-1]
NP_001154831.1. NM_001161359.1. [O14526-3]
NP_055937.1. NM_015122.2. [O14526-1]
XP_006722764.1. XM_006722701.1. [O14526-1]
XP_006722765.1. XM_006722702.1. [O14526-1]
UniGeneHs.96485.

3D structure databases

ProteinModelPortalO14526.
SMRO14526. Positions 1-269.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116764. 4 interactions.
IntActO14526. 4 interactions.
MINTMINT-7979234.
STRING9606.ENSP00000252771.

PTM databases

PhosphoSiteO14526.

Proteomic databases

MaxQBO14526.
PaxDbO14526.
PRIDEO14526.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000252771; ENSP00000252771; ENSG00000130475. [O14526-1]
ENST00000389133; ENSP00000373785; ENSG00000130475. [O14526-1]
ENST00000539407; ENSP00000437978; ENSG00000130475. [O14526-1]
ENST00000594202; ENSP00000473001; ENSG00000130475.
ENST00000595033; ENSP00000472668; ENSG00000130475. [O14526-3]
ENST00000596536; ENSP00000470731; ENSG00000130475. [O14526-1]
ENST00000596951; ENSP00000472417; ENSG00000130475. [O14526-1]
ENST00000600676; ENSP00000470493; ENSG00000130475. [O14526-1]
GeneID23149.
KEGGhsa:23149.
UCSCuc002nhg.3. human. [O14526-2]
uc002nhh.2. human. [O14526-1]

Organism-specific databases

CTD23149.
GeneCardsGC19P017862.
HGNCHGNC:29002. FCHO1.
HPAHPA041653.
MIM613437. gene.
neXtProtNX_O14526.
PharmGKBPA134870625.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG324072.
HOGENOMHOG000231544.
HOVERGENHBG081524.
InParanoidO14526.
OrthoDBEOG712TVK.
PhylomeDBO14526.
TreeFamTF328986.

Gene expression databases

ArrayExpressO14526.
BgeeO14526.
CleanExHS_FCHO1.
GenevestigatorO14526.

Family and domain databases

InterProIPR001060. FCH_dom.
IPR028565. MHD.
IPR018808. Muniscin_C.
[Graphical view]
PfamPF00611. FCH. 1 hit.
PF10291. muHD. 1 hit.
[Graphical view]
SMARTSM00055. FCH. 1 hit.
[Graphical view]
PROSITEPS50133. FCH. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSFCHO1. human.
GenomeRNAi23149.
NextBio44466.
PROO14526.
SOURCESearch...

Entry information

Entry nameFCHO1_HUMAN
AccessionPrimary (citable) accession number: O14526
Secondary accession number(s): A6NHE6 expand/collapse secondary AC list , A8K5U5, B4E120, Q05C93, Q8IW22
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: January 9, 2007
Last modified: July 9, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM