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Protein

Nuclear envelope integral membrane protein 1

Gene

NEMP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Enzyme and pathway databases

BioCyciZFISH:ENSG00000166881-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear envelope integral membrane protein 1
Gene namesi
Name:NEMP1
Synonyms:KIAA0286, TMEM194, TMEM194A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:29001. NEMP1.

Subcellular locationi

  • Nucleus inner membrane By similarity; Multi-pass membrane protein Sequence analysis; Nucleoplasmic side By similarity
  • Nucleus envelope By similarity

  • Note: Colocalizes with lamins and RAN-GTP at the nuclear envelope.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei161 – 181HelicalSequence analysisAdd BLAST21
Transmembranei186 – 206HelicalSequence analysisAdd BLAST21
Transmembranei216 – 236HelicalSequence analysisAdd BLAST21
Transmembranei245 – 265HelicalSequence analysisAdd BLAST21
Transmembranei289 – 309HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000166881.
PharmGKBiPA162406290.

Polymorphism and mutation databases

BioMutaiTMEM194A.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 43Sequence analysisAdd BLAST43
ChainiPRO_000005074444 – 444Nuclear envelope integral membrane protein 1Add BLAST401

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi125N-linked (GlcNAc...)Sequence analysis1
Modified residuei368PhosphoserineCombined sources1
Modified residuei424PhosphoserineCombined sources1
Modified residuei425PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation may regulate its interaction with RAN-GTP.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiO14524.
MaxQBiO14524.
PaxDbiO14524.
PeptideAtlasiO14524.
PRIDEiO14524.

PTM databases

iPTMnetiO14524.
PhosphoSitePlusiO14524.

Expressioni

Gene expression databases

BgeeiENSG00000166881.
CleanExiHS_TMEM194A.
ExpressionAtlasiO14524. baseline and differential.
GenevisibleiO14524. HS.

Organism-specific databases

HPAiHPA014394.

Interactioni

Subunit structurei

Homooligomer. Interacts with RAN-GTP.By similarity

Protein-protein interaction databases

BioGridi116898. 25 interactors.
IntActiO14524. 7 interactors.
STRINGi9606.ENSP00000300128.

Structurei

3D structure databases

ProteinModelPortaliO14524.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni186 – 297A; required for its colocalization with lamins at the nuclear envelopeBy similarityAdd BLAST112
Regioni336 – 444Required for nuclear localizationBy similarityAdd BLAST109
Regioni336 – 405B; required for interaction with RAN-GTPBy similarityAdd BLAST70

Domaini

The transmembrane domains are required and sufficient for its oligomerization.By similarity

Sequence similaritiesi

Belongs to the NEMP family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3817. Eukaryota.
ENOG410ZZKM. LUCA.
GeneTreeiENSGT00390000002174.
HOGENOMiHOG000122573.
HOVERGENiHBG052180.
InParanoidiO14524.
OMAiCPAITQN.
OrthoDBiEOG091G09L2.
PhylomeDBiO14524.
TreeFamiTF314831.

Family and domain databases

InterProiIPR019358. NEMP_fam.
[Graphical view]
PfamiPF10225. NEMP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O14524-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGGMKVAVS PAVGPGPWGS GVGGGGTVRL LLILSGCLVY GTAETDVNVV
60 70 80 90 100
MLQESQVCEK RASQQFCYTN VLIPKWHDIW TRIQIRVNSS RLVRVTQVEN
110 120 130 140 150
EEKLKELEQF SIWNFFSSFL KEKLNDTYVN VGLYSTKTCL KVEIIEKDTK
160 170 180 190 200
YSVIVIRRFD PKLFLVFLLG LMLFFCGDLL SRSQIFYYST GMTVGIVASL
210 220 230 240 250
LIIIFILSKF MPKKSPIYVI LVGGWSFSLY LIQLVFKNLQ EIWRCYWQYL
260 270 280 290 300
LSYVLTVGFM SFAVCYKYGP LENERSINLL TWTLQLMGLC FMYSGIQIPH
310 320 330 340 350
IALAIIIIAL CTKNLEHPIQ WLYITCRKVC KGAEKPVPPR LLTEEEYRIQ
360 370 380 390 400
GEVETRKALE ELREFCNSPD CSAWKTVSRI QSPKRFADFV EGSSHLTPNE
410 420 430 440
VSVHEQEYGL GSIIAQDEIY EEASSEEEDS YSRCPAITQN NFLT
Length:444
Mass (Da):50,640
Last modified:November 23, 2004 - v2
Checksum:iF45D4D3F9B86C77B
GO
Isoform 2 (identifier: O14524-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     110-182: Missing.

Note: No experimental confirmation available.
Show »
Length:371
Mass (Da):42,033
Checksum:iBD222DDE1AB196A5
GO

Sequence cautioni

The sequence AAH71597 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAA22955 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti442F → L in CAH18250 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_057817217I → V.Corresponds to variant rs17546579dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_011896110 – 182Missing in isoform 2. 3 PublicationsAdd BLAST73

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006624 mRNA. Translation: BAA22955.2. Different initiation.
CR749406 mRNA. Translation: CAH18250.1.
AK294566 mRNA. Translation: BAG57761.1.
BC071597 mRNA. Translation: AAH71597.1. Different initiation.
BC117436 mRNA. Translation: AAI17437.1.
BC126300 mRNA. Translation: AAI26301.1.
CCDSiCCDS31841.1. [O14524-2]
CCDS44927.1. [O14524-1]
RefSeqiNP_001124435.1. NM_001130963.1. [O14524-1]
NP_056072.2. NM_015257.2. [O14524-2]
UniGeneiHs.591040.

Genome annotation databases

EnsembliENST00000300128; ENSP00000300128; ENSG00000166881. [O14524-1]
ENST00000379391; ENSP00000368701; ENSG00000166881. [O14524-2]
GeneIDi23306.
KEGGihsa:23306.
UCSCiuc001smx.4. human. [O14524-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006624 mRNA. Translation: BAA22955.2. Different initiation.
CR749406 mRNA. Translation: CAH18250.1.
AK294566 mRNA. Translation: BAG57761.1.
BC071597 mRNA. Translation: AAH71597.1. Different initiation.
BC117436 mRNA. Translation: AAI17437.1.
BC126300 mRNA. Translation: AAI26301.1.
CCDSiCCDS31841.1. [O14524-2]
CCDS44927.1. [O14524-1]
RefSeqiNP_001124435.1. NM_001130963.1. [O14524-1]
NP_056072.2. NM_015257.2. [O14524-2]
UniGeneiHs.591040.

3D structure databases

ProteinModelPortaliO14524.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116898. 25 interactors.
IntActiO14524. 7 interactors.
STRINGi9606.ENSP00000300128.

PTM databases

iPTMnetiO14524.
PhosphoSitePlusiO14524.

Polymorphism and mutation databases

BioMutaiTMEM194A.

Proteomic databases

EPDiO14524.
MaxQBiO14524.
PaxDbiO14524.
PeptideAtlasiO14524.
PRIDEiO14524.

Protocols and materials databases

DNASUi23306.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000300128; ENSP00000300128; ENSG00000166881. [O14524-1]
ENST00000379391; ENSP00000368701; ENSG00000166881. [O14524-2]
GeneIDi23306.
KEGGihsa:23306.
UCSCiuc001smx.4. human. [O14524-1]

Organism-specific databases

CTDi23306.
GeneCardsiTMEM194A.
H-InvDBHIX0010743.
HGNCiHGNC:29001. NEMP1.
HPAiHPA014394.
MIMi616496. gene.
neXtProtiNX_O14524.
OpenTargetsiENSG00000166881.
PharmGKBiPA162406290.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3817. Eukaryota.
ENOG410ZZKM. LUCA.
GeneTreeiENSGT00390000002174.
HOGENOMiHOG000122573.
HOVERGENiHBG052180.
InParanoidiO14524.
OMAiCPAITQN.
OrthoDBiEOG091G09L2.
PhylomeDBiO14524.
TreeFamiTF314831.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000166881-MONOMER.

Miscellaneous databases

GenomeRNAii23306.
PROiO14524.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000166881.
CleanExiHS_TMEM194A.
ExpressionAtlasiO14524. baseline and differential.
GenevisibleiO14524. HS.

Family and domain databases

InterProiIPR019358. NEMP_fam.
[Graphical view]
PfamiPF10225. NEMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNEMP1_HUMAN
AccessioniPrimary (citable) accession number: O14524
Secondary accession number(s): Q17R72, Q68DH0, Q6IQ25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 23, 2004
Last modified: November 2, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.