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O14519 (CDKA1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 2-associated protein 1

Short name=CDK2-associated protein 1
Alternative name(s):
Deleted in oral cancer 1
Short name=DOC-1
Putative oral cancer suppressor
Gene names
Name:CDK2AP1
Synonyms:CDKAP1, DOC1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length115 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

specific inhibitor of the cell-cycle kinase CDK2 By similarity.

Subunit structure

Homodimer. Interacts with monomeric unphosphorylated CDK2. Ref.6

Post-translational modification

Phosphorylated in vitro by IKBKE at Ser-46. Ref.6

Sequence similarities

Belongs to the CDK2AP family.

Ontologies

Keywords
   Biological processCell cycle
   Coding sequence diversityAlternative splicing
   DiseaseTumor suppressor
   PTMDisulfide bond
Phosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA-dependent DNA replication

Traceable author statement Ref.2. Source: ProtInc

mitotic S phase

Traceable author statement Ref.2. Source: ProtInc

protein phosphorylation

Traceable author statement Ref.2. Source: ProtInc

   Cellular_componentcytoplasm

Traceable author statement Ref.2. Source: ProtInc

nucleus

Traceable author statement Ref.2. Source: ProtInc

   Molecular_functionDNA binding

Traceable author statement Ref.2. Source: ProtInc

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O14519-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O14519-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 115115Cyclin-dependent kinase 2-associated protein 1
PRO_0000089452

Regions

Region1 – 6060intrinsically disordered

Amino acid modifications

Modified residue461Phosphoserine; by IKKE Ref.6
Disulfide bond105Interchain Potential

Natural variations

Alternative sequence1 – 2828Missing in isoform 2.
VSP_046436

Experimental info

Mutagenesis1051C → A: Does not alter homodimerization. Ref.6

Secondary structure

........ 115
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: F3149F72467598C2

FASTA11512,365
        10         20         30         40         50         60 
MSYKPNLAAH MPAAALNAAG SVHSPSTSMA TSSQYRQLLS DYGPPSLGYT QGTGNSQVPQ 

        70         80         90        100        110 
SKYAELLAII EELGKEIRPT YAGSKSAMER LKRGIIHARG LVRECLAETE RNARS 

« Hide

Isoform 2 [UniParc].

Checksum: 6B823B0C04D5D88A
Show »

FASTA879,601

References

« Hide 'large scale' references
[1]"Isolation, mapping and mutation analysis of a human cDNA homologous to the doc-1 gene of the Chinese hamster, a candidate tumor suppressor for oral cancer."
Daigo Y., Suzuki K., Maruyama O., Miyoshi Y., Yasuda T., Kabuto T., Imaoka S., Fujiwara T., Takahashi E., Fujino M.A., Nakamura Y.
Genes Chromosomes Cancer 20:204-207(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Cloning, mapping, expression, function, and mutation analyses of the human ortholog of the hamster putative tumor suppressor gene Doc-1."
Tsuji T., Duh F.-M., Latif F., Popescu N.C., Zimonjic D.B., McBride J., Matsuo K., Ohyama H., Todd R., Nagata E., Terakado N., Sasaki A., Matsumura T., Lerman M.I., Wong D.T.
J. Biol. Chem. 273:6704-6709(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Testis.
[3]Arakawa T., Carninci P., Fukuda S., Hasegawa A., Hayashida K., Hori F., Kai C., Kawai J., Kojima M., Murata M., Nakamura M., Nishiyori H., Nomura K., Ohno M., Sasaki D., Shibazaki E., Tagami M., Tagami Y., Hayashizaki Y.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]"The finished DNA sequence of human chromosome 12."
Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. expand/collapse author list , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Skin.
[6]"Human cyclin-dependent kinase 2-associated protein 1 (CDK2AP1) is dimeric in its disulfide-reduced state, with natively disordered N-terminal region."
Ertekin A., Aramini J.M., Rossi P., Leonard P.G., Janjua H., Xiao R., Maglaqui M., Lee H.W., Prestegard J.H., Montelione G.T.
J. Biol. Chem. 287:16541-16549(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 61-115, SUBUNIT, PHOSPHORYLATION AT SER-46, DISULFIDE BOND, MUTAGENESIS OF CYS-105.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB006077 mRNA. Translation: BAA22937.1.
AF006484 mRNA. Translation: AAC77831.1.
DB465022 mRNA. No translation available.
AC068768 Genomic DNA. No translation available.
BC034717 mRNA. Translation: AAH34717.1.
CCDSCCDS58289.1. [O14519-2]
CCDS9245.1. [O14519-1]
RefSeqNP_001257362.1. NM_001270433.1. [O14519-2]
NP_001257363.1. NM_001270434.1. [O14519-2]
NP_004633.1. NM_004642.3. [O14519-1]
UniGeneHs.433201.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2KW6NMR-A/B61-115[»]
ProteinModelPortalO14519.
SMRO14519. Positions 62-115.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113770. 19 interactions.
IntActO14519. 5 interactions.
MINTMINT-88804.
STRING9606.ENSP00000261692.

Chemistry

BindingDBO14519.
ChEMBLCHEMBL5578.

PTM databases

PhosphoSiteO14519.

Proteomic databases

MaxQBO14519.
PaxDbO14519.
PRIDEO14519.

Protocols and materials databases

DNASU8099.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000261692; ENSP00000261692; ENSG00000111328. [O14519-1]
ENST00000535979; ENSP00000442565; ENSG00000111328. [O14519-2]
ENST00000538446; ENSP00000442502; ENSG00000111328. [O14519-2]
ENST00000542174; ENSP00000440729; ENSG00000111328. [O14519-2]
ENST00000544658; ENSP00000438561; ENSG00000111328. [O14519-2]
GeneID8099.
KEGGhsa:8099.
UCSCuc001ueq.4. human. [O14519-1]

Organism-specific databases

CTD8099.
GeneCardsGC12M123745.
HGNCHGNC:14002. CDK2AP1.
HPACAB019377.
MIM602198. gene.
neXtProtNX_O14519.
PharmGKBPA26308.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG235568.
HOGENOMHOG000035115.
HOVERGENHBG002074.
InParanoidO14519.
OMACAICTHY.
OrthoDBEOG71RXN6.
PhylomeDBO14519.
TreeFamTF101037.

Gene expression databases

ArrayExpressO14519.
BgeeO14519.
CleanExHS_CDK2AP1.
GenevestigatorO14519.

Family and domain databases

InterProIPR017266. CDK2-associated_2.
IPR019187. Cyclin-dep_kinase2-assoc_pr.
[Graphical view]
PfamPF09806. CDK2AP. 1 hit.
[Graphical view]
PIRSFPIRSF037709. CDK2-associated_p2. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceO14519.
GeneWikiCDK2AP1.
GenomeRNAi8099.
NextBio30740.
PROO14519.
SOURCESearch...

Entry information

Entry nameCDKA1_HUMAN
AccessionPrimary (citable) accession number: O14519
Secondary accession number(s): F5GYA4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM