Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Adhesion G protein-coupled receptor B1

Gene

ADGRB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphatidylserine receptor which enhances the engulfment of apoptotic cells (PubMed:24509909). Also mediates the binding and engulfment of Gram-negative bacteria (PubMed:26838550). Stimulates production of reactive oxygen species by macrophages in response to Gram-negative bacteria, resulting in enhanced microbicidal macrophage activity (PubMed:26838550). In the gastric mucosa, required for recognition and engulfment of apoptotic gastric epithelial cells (PubMed:24509909). Promotes myoblast fusion (By similarity). Activates the Rho pathway in a G-protein-dependent manner (PubMed:23782696). Inhibits MDM2-mediated ubiquitination and degradation of DLG4/PSD95, promoting DLG4 stability and regulating synaptic plasticity (By similarity). Required for the formation of dendritic spines by ensuring the correct localization of PARD3 and TIAM1 (By similarity). Potent inhibitor of angiogenesis in brain and may play a significant role as a mediator of the p53/TP53 signal in suppression of glioblastoma (PubMed:11875720).By similarity4 Publications
Vasculostatin-120: Inhibits angiogenesis in a CD36-dependent manner.2 Publications
Vasculostatin-40: Inhibits angiogenesis.1 Publication

GO - Molecular functioni

GO - Biological processi

  • apoptotic cell clearance Source: UniProtKB
  • axonogenesis Source: ProtInc
  • cell adhesion Source: ProtInc
  • cell surface receptor signaling pathway Source: InterPro
  • defense response to Gram-negative bacterium Source: UniProtKB
  • engulfment of apoptotic cell Source: UniProtKB
  • G-protein coupled receptor signaling pathway Source: GDB
  • innate immune response Source: UniProtKB-KW
  • muscle organ development Source: UniProtKB-KW
  • negative regulation of angiogenesis Source: UniProtKB
  • negative regulation of cell proliferation Source: ProtInc
  • negative regulation of endothelial cell migration Source: UniProtKB
  • negative regulation of protein catabolic process Source: UniProtKB
  • negative regulation of protein ubiquitination Source: UniProtKB
  • peripheral nervous system development Source: ProtInc
  • phagocytosis, recognition Source: UniProtKB
  • positive regulation of myoblast fusion Source: UniProtKB
  • positive regulation of reactive oxygen species biosynthetic process Source: UniProtKB
  • positive regulation of synapse assembly Source: Ensembl
  • regulation of synaptic plasticity Source: UniProtKB
  • signal transduction Source: ProtInc

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processImmunity, Innate immunity, Myogenesis, Neurogenesis, Phagocytosis

Enzyme and pathway databases

SignaLinkiO14514.

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesion G protein-coupled receptor B1Imported
Alternative name(s):
Brain-specific angiogenesis inhibitor 11 Publication
Cleaved into the following 2 chains:
Vasculostatin-401 Publication
Short name:
Vstat401 Publication
Vasculostatin-1201 Publication
Short name:
Vstat1201 Publication
Gene namesi
Name:ADGRB1Imported
Synonyms:BAI11 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000181790.10.
HGNCiHGNC:943. ADGRB1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini31 – 948ExtracellularSequence analysisAdd BLAST918
Transmembranei949 – 969Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini970 – 980CytoplasmicSequence analysisAdd BLAST11
Transmembranei981 – 1001Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini1002 – 1008ExtracellularSequence analysis7
Transmembranei1009 – 1029Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini1030 – 1052CytoplasmicSequence analysisAdd BLAST23
Transmembranei1053 – 1073Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini1074 – 1093ExtracellularSequence analysisAdd BLAST20
Transmembranei1094 – 1114Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini1115 – 1136CytoplasmicSequence analysisAdd BLAST22
Transmembranei1137 – 1157Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini1158 – 1166ExtracellularSequence analysis9
Transmembranei1167 – 1187Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini1188 – 1584CytoplasmicSequence analysisAdd BLAST397

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane, Postsynaptic cell membrane, Secreted, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi323 – 325RSQ → AAA: Abolishes processing of vasculostatin-40. 1 Publication3
Mutagenesisi326 – 328SLR → AAA: Does not affect processing of vasculostatin-40. 1 Publication3
Mutagenesisi927S → A: Abolishes cleavage and production of vasculostatin-120. 1 Publication1
Mutagenesisi927S → D: Increased levels of vasculostatin-120 and decreased levels of vasculostatin-40. 1 Publication1

Organism-specific databases

DisGeNETi575.
OpenTargetsiENSG00000181790.
PharmGKBiPA25247.

Polymorphism and mutation databases

BioMutaiBAI1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000001286331 – 1584Adhesion G protein-coupled receptor B1Add BLAST1554
ChainiPRO_000044180531 – 926Vasculostatin-1201 PublicationAdd BLAST896
ChainiPRO_000044180431 – ?327Vasculostatin-401 PublicationAdd BLAST297

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi64N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi273 ↔ 309
Disulfide bondi277 ↔ 314
Disulfide bondi288 ↔ 299
Disulfide bondi366 ↔ 400
Disulfide bondi370 ↔ 406
Disulfide bondi381 ↔ 390
Glycosylationi401N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi421 ↔ 456
Disulfide bondi425 ↔ 461
Disulfide bondi436 ↔ 446
Disulfide bondi479 ↔ 514
Disulfide bondi483 ↔ 519
Disulfide bondi494 ↔ 504
Disulfide bondi534 ↔ 569
Disulfide bondi538 ↔ 574
Disulfide bondi549 ↔ 559
Disulfide bondi581 ↔ 616
Disulfide bondi604 ↔ 634
Glycosylationi607N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei609PhosphothreonineCombined sources1
Glycosylationi692N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi844N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi877N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi881N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi884 ↔ 921
Disulfide bondi909 ↔ 923
Modified residuei1469PhosphoserineBy similarity1

Post-translational modificationi

Proteolytically cleaved to produce vasculostatin-40 and vasculostatin-120 (PubMed:15782143, PubMed:22333914, PubMed:22330140). Vasculostatin-40 is the major form and is produced through proteolytic cleavage by MMP14 between residues 321 and 329 with cleavage likely to be between Ser-326 and Leu-327 (PubMed:22330140).3 Publications
Ubiquitinated.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei926 – 927Cleavage1 Publication2

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO14514.
PaxDbiO14514.
PeptideAtlasiO14514.
PRIDEiO14514.

PTM databases

iPTMnetiO14514.
PhosphoSitePlusiO14514.

Expressioni

Tissue specificityi

Expressed in brain (at protein level) (PubMed:12074842, PubMed:12507886). Expressed on mononuclear phagocytes and monocyte-derived macrophages in the gastric mucosa (at protein level) (PubMed:24509909). Expressed in normal pancreatic tissue but not in pancreatic tumor tissue (PubMed:11875720). Reduced or no expression is observed in some glioblastomas (PubMed:12507886).4 Publications

Inductioni

By p53/TP53.

Gene expression databases

BgeeiENSG00000181790.
CleanExiHS_BAI1.
ExpressionAtlasiO14514. baseline and differential.
GenevisibleiO14514. HS.

Interactioni

Subunit structurei

Interacts with ELMO1 and DOCK (By similarity). When bound to ELMO1 and DOCK1, acts as a module to promote apoptotic cell engulfment (By similarity). Interacts with MDM2; the interaction results in inhibition of MDM2-mediated ubiquitination and degradation of DLG4/PSD95 (By similarity). Interacts with PARD3 and TIAM1; the interaction is required for correct dendritic. localization of PARD3 and TIAM1 and for dendritic spine formation (PubMed:23595754). Interacts with MAGI1 (PubMed:9647739). Interacts with MAGI3 (PubMed:10748157). Interacts with BAIAP2 (PubMed:10343108). Interacts with PHYHIP (By similarity). Interacts with DLG4 (via PDZ domain) (PubMed:23782696). Vasculostatin-120: Interacts with CD36 (PubMed:19176395). Vasculostatin-120: Interacts with ARRB2 (PubMed:23782696).By similarity6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ELMO1Q92556-12EBI-1995178,EBI-15668002

GO - Molecular functioni

Protein-protein interaction databases

BioGridi107051. 20 interactors.
DIPiDIP-40884N.
IntActiO14514. 4 interactors.
MINTiMINT-93782.
STRINGi9606.ENSP00000313046.

Structurei

3D structure databases

ProteinModelPortaliO14514.
SMRiO14514.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini261 – 315TSP type-1 1Add BLAST55
Domaini354 – 407TSP type-1 2Add BLAST54
Domaini409 – 462TSP type-1 3Add BLAST54
Domaini467 – 520TSP type-1 4Add BLAST54
Domaini522 – 575TSP type-1 5Add BLAST54
Domaini881 – 938GPSAdd BLAST58

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni927 – 943N-terminal stalk following vasculostatin-120 cleavage which is not required for signaling activity1 PublicationAdd BLAST17
Regioni1365 – 1584Involved in interaction with MAGI11 PublicationAdd BLAST220
Regioni1581 – 1584Indispensable for interaction with MAGI11 Publication4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1411 – 1422Poly-ProAdd BLAST12
Compositional biasi1425 – 1430Poly-Pro6

Domaini

The TSP type-1 repeats in the extracellular domain mediate binding to phosphatidylserine. They are also required for bacterial recognition and binding to bacterial outer membrane lipopolysaccharide.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEA0. Eukaryota.
ENOG4111FBM. LUCA.
GeneTreeiENSGT00900000140832.
HOGENOMiHOG000230916.
HOVERGENiHBG004813.
InParanoidiO14514.
KOiK04596.
OMAiSWRGCRT.
OrthoDBiEOG091G00C7.
PhylomeDBiO14514.
TreeFamiTF331634.

Family and domain databases

Gene3Di2.20.100.10. 5 hits.
InterProiView protein in InterPro
IPR032471. GAIN_dom_N.
IPR017981. GPCR_2-like.
IPR008077. GPCR_2_brain_angio_inhib.
IPR001879. GPCR_2_extracellular_dom.
IPR000832. GPCR_2_secretin-like.
IPR000203. GPS.
IPR000884. TSP1_rpt.
IPR036383. TSP1_rpt_sf.
PfamiView protein in Pfam
PF00002. 7tm_2. 1 hit.
PF16489. GAIN. 1 hit.
PF01825. GPS. 1 hit.
PF02793. HRM. 1 hit.
PF00090. TSP_1. 5 hits.
PRINTSiPR01694. BAIPRECURSOR.
PR00249. GPCRSECRETIN.
SMARTiView protein in SMART
SM00303. GPS. 1 hit.
SM00008. HormR. 1 hit.
SM00209. TSP1. 5 hits.
SUPFAMiSSF82895. SSF82895. 5 hits.
PROSITEiView protein in PROSITE
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS50092. TSP1. 5 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O14514-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGQAAAPGP VWILAPLLLL LLLLGRRARA AAGADAGPGP EPCATLVQGK
60 70 80 90 100
FFGYFSAAAV FPANASRCSW TLRNPDPRRY TLYMKVAKAP VPCSGPGRVR
110 120 130 140 150
TYQFDSFLES TRTYLGVESF DEVLRLCDPS APLAFLQASK QFLQMRRQQP
160 170 180 190 200
PQHDGLRPRA GPPGPTDDFS VEYLVVGNRN PSRAACQMLC RWLDACLAGS
210 220 230 240 250
RSSHPCGIMQ TPCACLGGEA GGPAAGPLAP RGDVCLRDAV AGGPENCLTS
260 270 280 290 300
LTQDRGGHGA TGGWKLWSLW GECTRDCGGG LQTRTRTCLP APGVEGGGCE
310 320 330 340 350
GVLEEGRQCN REACGPAGRT SSRSQSLRST DARRREELGD ELQQFGFPAP
360 370 380 390 400
QTGDPAAEEW SPWSVCSSTC GEGWQTRTRF CVSSSYSTQC SGPLREQRLC
410 420 430 440 450
NNSAVCPVHG AWDEWSPWSL CSSTCGRGFR DRTRTCRPPQ FGGNPCEGPE
460 470 480 490 500
KQTKFCNIAL CPGRAVDGNW NEWSSWSACS ASCSQGRQQR TRECNGPSYG
510 520 530 540 550
GAECQGHWVE TRDCFLQQCP VDGKWQAWAS WGSCSVTCGA GSQRRERVCS
560 570 580 590 600
GPFFGGAACQ GPQDEYRQCG TQRCPEPHEI CDEDNFGAVI WKETPAGEVA
610 620 630 640 650
AVRCPRNATG LILRRCELDE EGIAYWEPPT YIRCVSIDYR NIQMMTREHL
660 670 680 690 700
AKAQRGLPGE GVSEVIQTLV EISQDGTSYS GDLLSTIDVL RNMTEIFRRA
710 720 730 740 750
YYSPTPGDVQ NFVQILSNLL AEENRDKWEE AQLAGPNAKE LFRLVEDFVD
760 770 780 790 800
VIGFRMKDLR DAYQVTDNLV LSIHKLPASG ATDISFPMKG WRATGDWAKV
810 820 830 840 850
PEDRVTVSKS VFSTGLTEAD EASVFVVGTV LYRNLGSFLA LQRNTTVLNS
860 870 880 890 900
KVISVTVKPP PRSLRTPLEI EFAHMYNGTT NQTCILWDET DVPSSSAPPQ
910 920 930 940 950
LGPWSWRGCR TVPLDALRTR CLCDRLSTFA ILAQLSADAN MEKATLPSVT
960 970 980 990 1000
LIVGCGVSSL TLLMLVIIYV SVWRYIRSER SVILINFCLS IISSNALILI
1010 1020 1030 1040 1050
GQTQTRNKVV CTLVAAFLHF FFLSSFCWVL TEAWQSYMAV TGHLRNRLIR
1060 1070 1080 1090 1100
KRFLCLGWGL PALVVAISVG FTKAKGYSTM NYCWLSLEGG LLYAFVGPAA
1110 1120 1130 1140 1150
AVVLVNMVIG ILVFNKLVSK DGITDKKLKE RAGASLWSSC VVLPLLALTW
1160 1170 1180 1190 1200
MSAVLAVTDR RSALFQILFA VFDSLEGFVI VMVHCILRRE VQDAVKCRVV
1210 1220 1230 1240 1250
DRQEEGNGDS GGSFQNGHAQ LMTDFEKDVD LACRSVLNKD IAACRTATIT
1260 1270 1280 1290 1300
GTLKRPSLPE EEKLKLAHAK GPPTNFNSLP ANVSKLHLHG SPRYPGGPLP
1310 1320 1330 1340 1350
DFPNHSLTLK RDKAPKSSFV GDGDIFKKLD SELSRAQEKA LDTSYVILPT
1360 1370 1380 1390 1400
ATATLRPKPK EEPKYSIHID QMPQTRLIHL STAPEASLPA RSPPSRQPPS
1410 1420 1430 1440 1450
GGPPEAPPAQ PPPPPPPPPP PPQQPLPPPP NLEPAPPSLG DPGEPAAHPG
1460 1470 1480 1490 1500
PSTGPSTKNE NVATLSVSSL ERRKSRYAEL DFEKIMHTRK RHQDMFQDLN
1510 1520 1530 1540 1550
RKLQHAAEKD KEVLGPDSKP EKQQTPNKRP WESLRKAHGT PTWVKKELEP
1560 1570 1580
LQPSPLELRS VEWERSGATI PLVGQDIIDL QTEV
Length:1,584
Mass (Da):173,501
Last modified:October 31, 2012 - v2
Checksum:iA604E634DDD741F7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1010V → M in BAA23647 (PubMed:9393972).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005297 mRNA. Translation: BAA23647.1.
AC139676 Genomic DNA. No translation available.
CCDSiCCDS64985.1.
PIRiT00026.
RefSeqiNP_001693.2. NM_001702.2.
XP_011515501.1. XM_011517199.2.
UniGeneiHs.194654.

Genome annotation databases

EnsembliENST00000323289; ENSP00000313046; ENSG00000181790.
ENST00000517894; ENSP00000430945; ENSG00000181790.
GeneIDi575.
KEGGihsa:575.
UCSCiuc003ywm.4. human.

Similar proteinsi

Entry informationi

Entry nameiAGRB1_HUMAN
AccessioniPrimary (citable) accession number: O14514
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 31, 2012
Last modified: November 22, 2017
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. SIMILARITY comments
    Index of protein domains and families