O14514 (BAI1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Brain-specific angiogenesis inhibitor 1

Gene names

Name:

BAI1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1584 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Likely to be a potent inhibitor of angiogenesis in brain and may play a significant role as a mediator of the p53 signal in suppression of glioblastoma. May function in cell adhesion and signal transduction in the brain. Ref.6
Subunit structure	Interacts with ELMO1 and DOCK1. When bound to ELMO1 and DOCK1, it may act as a module to promote the engulfment By similarity. Interacts with MAGI1, MAGI3, BAIAP2 and PHYHIP. Ref.3 Ref.4 Ref.5
Subcellular location	Cell membrane; Multi-pass membrane protein. Note: Likely to be concentrated at cell-cell adhesion sites. Ref.7
Tissue specificity	Specifically expressed in brain. Reduced or no expression is observed in some glioblastoma cell lines and cancer tissues. No expression in astrocytes. Ref.6 Ref.7 Ref.8
Induction	By p53/TP53.
Domain	The TSP1 repeats inhibit in vivo angiogenesis in rat cornea induced by BFGF.
Post-translational modification	The endogenous protein is proteolytically cleaved into 2 subunits, an extracellular subunit and a seven-transmembrane subunit. Ref.9
Sequence similarities	Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. Contains 1 GPS domain. Contains 5 TSP type-1 domains.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Membrane
Domain	Repeat Signal Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axonogenesis Traceable author statement Ref.4. Source: ProtInc cell adhesion Traceable author statement Ref.3. Source: ProtInc negative regulation of angiogenesis Inferred from electronic annotation. Source: InterPro negative regulation of cell proliferation Traceable author statement PubMed 7531056. Source: ProtInc neuropeptide signaling pathway Inferred from electronic annotation. Source: InterPro peripheral nervous system development Traceable author statement Ref.4. Source: ProtInc
Cellular_component	cell-cell junction Traceable author statement Ref.3. Source: ProtInc integral to plasma membrane Traceable author statement Ref.3. Source: ProtInc
Molecular_function	G-protein coupled receptor activity Traceable author statement PubMed 15203201. Source: GDB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 1584	1554	Brain-specific angiogenesis inhibitor 1		PRO_0000012863
Regions
	Topological domain	31 – 948	918	Extracellular Potential
	Transmembrane	949 – 969	21	Helical; Name=1; Potential
	Topological domain	970 – 980	11	Cytoplasmic Potential
	Transmembrane	981 – 1001	21	Helical; Name=2; Potential
	Topological domain	1002 – 1008	7	Extracellular Potential
	Transmembrane	1009 – 1029	21	Helical; Name=3; Potential
	Topological domain	1030 – 1052	23	Cytoplasmic Potential
	Transmembrane	1053 – 1073	21	Helical; Name=4; Potential
	Topological domain	1074 – 1093	20	Extracellular Potential
	Transmembrane	1094 – 1114	21	Helical; Name=5; Potential
	Topological domain	1115 – 1136	22	Cytoplasmic Potential
	Transmembrane	1137 – 1157	21	Helical; Name=6; Potential
	Topological domain	1158 – 1166	9	Extracellular Potential
	Transmembrane	1167 – 1187	21	Helical; Name=7; Potential
	Topological domain	1188 – 1584	397	Cytoplasmic Potential
	Domain	261 – 315	55	TSP type-1 1
	Domain	354 – 407	54	TSP type-1 2
	Domain	409 – 462	54	TSP type-1 3
	Domain	467 – 520	54	TSP type-1 4
	Domain	522 – 575	54	TSP type-1 5
	Domain	881 – 938	58	GPS
	Region	1365 – 1584	220	Necessary for interaction with MAGI1
	Region	1581 – 1584	4	Indispensable for interaction with MAGI1
	Motif	231 – 233	3	Cell attachment site Potential
	Compositional bias	1411 – 1422	12	Poly-Pro
	Compositional bias	1425 – 1430	6	Poly-Pro
Amino acid modifications
	Modified residue	1278	1	Phosphoserine By similarity
	Modified residue	1469	1	Phosphoserine By similarity
	Glycosylation	64	1	N-linked (GlcNAc...) Potential
	Glycosylation	401	1	N-linked (GlcNAc...) Potential
	Glycosylation	607	1	N-linked (GlcNAc...) Potential
	Glycosylation	692	1	N-linked (GlcNAc...) Potential
	Glycosylation	844	1	N-linked (GlcNAc...) Potential
	Glycosylation	877	1	N-linked (GlcNAc...) Potential
	Glycosylation	881	1	N-linked (GlcNAc...) Potential
	Disulfide bond	273 ↔ 309		By similarity
	Disulfide bond	277 ↔ 314		By similarity
	Disulfide bond	288 ↔ 299		By similarity
	Disulfide bond	366 ↔ 400		By similarity
	Disulfide bond	370 ↔ 406		By similarity
	Disulfide bond	381 ↔ 390		By similarity
	Disulfide bond	421 ↔ 456		By similarity
	Disulfide bond	425 ↔ 461		By similarity
	Disulfide bond	436 ↔ 446		By similarity
	Disulfide bond	479 ↔ 514		By similarity
	Disulfide bond	483 ↔ 519		By similarity
	Disulfide bond	494 ↔ 504		By similarity
	Disulfide bond	534 ↔ 569		By similarity
	Disulfide bond	538 ↔ 574		By similarity
	Disulfide bond	549 ↔ 559		By similarity
	Disulfide bond	581 ↔ 616		By similarity
	Disulfide bond	604 ↔ 634		By similarity
	Disulfide bond	884 ↔ 921		By similarity
	Disulfide bond	909 ↔ 923		By similarity
Experimental info
	Sequence conflict	1010	1	V → M in BAA23647. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O14514 [UniParc]. Last modified October 31, 2012. Version 2. Checksum: A604E634DDD741F7	FASTA	1,584	173,501
	10 20 30 40 50 60 MRGQAAAPGP VWILAPLLLL LLLLGRRARA AAGADAGPGP EPCATLVQGK FFGYFSAAAV 70 80 90 100 110 120 FPANASRCSW TLRNPDPRRY TLYMKVAKAP VPCSGPGRVR TYQFDSFLES TRTYLGVESF 130 140 150 160 170 180 DEVLRLCDPS APLAFLQASK QFLQMRRQQP PQHDGLRPRA GPPGPTDDFS VEYLVVGNRN 190 200 210 220 230 240 PSRAACQMLC RWLDACLAGS RSSHPCGIMQ TPCACLGGEA GGPAAGPLAP RGDVCLRDAV 250 260 270 280 290 300 AGGPENCLTS LTQDRGGHGA TGGWKLWSLW GECTRDCGGG LQTRTRTCLP APGVEGGGCE 310 320 330 340 350 360 GVLEEGRQCN REACGPAGRT SSRSQSLRST DARRREELGD ELQQFGFPAP QTGDPAAEEW 370 380 390 400 410 420 SPWSVCSSTC GEGWQTRTRF CVSSSYSTQC SGPLREQRLC NNSAVCPVHG AWDEWSPWSL 430 440 450 460 470 480 CSSTCGRGFR DRTRTCRPPQ FGGNPCEGPE KQTKFCNIAL CPGRAVDGNW NEWSSWSACS 490 500 510 520 530 540 ASCSQGRQQR TRECNGPSYG GAECQGHWVE TRDCFLQQCP VDGKWQAWAS WGSCSVTCGA 550 560 570 580 590 600 GSQRRERVCS GPFFGGAACQ GPQDEYRQCG TQRCPEPHEI CDEDNFGAVI WKETPAGEVA 610 620 630 640 650 660 AVRCPRNATG LILRRCELDE EGIAYWEPPT YIRCVSIDYR NIQMMTREHL AKAQRGLPGE 670 680 690 700 710 720 GVSEVIQTLV EISQDGTSYS GDLLSTIDVL RNMTEIFRRA YYSPTPGDVQ NFVQILSNLL 730 740 750 760 770 780 AEENRDKWEE AQLAGPNAKE LFRLVEDFVD VIGFRMKDLR DAYQVTDNLV LSIHKLPASG 790 800 810 820 830 840 ATDISFPMKG WRATGDWAKV PEDRVTVSKS VFSTGLTEAD EASVFVVGTV LYRNLGSFLA 850 860 870 880 890 900 LQRNTTVLNS KVISVTVKPP PRSLRTPLEI EFAHMYNGTT NQTCILWDET DVPSSSAPPQ 910 920 930 940 950 960 LGPWSWRGCR TVPLDALRTR CLCDRLSTFA ILAQLSADAN MEKATLPSVT LIVGCGVSSL 970 980 990 1000 1010 1020 TLLMLVIIYV SVWRYIRSER SVILINFCLS IISSNALILI GQTQTRNKVV CTLVAAFLHF 1030 1040 1050 1060 1070 1080 FFLSSFCWVL TEAWQSYMAV TGHLRNRLIR KRFLCLGWGL PALVVAISVG FTKAKGYSTM 1090 1100 1110 1120 1130 1140 NYCWLSLEGG LLYAFVGPAA AVVLVNMVIG ILVFNKLVSK DGITDKKLKE RAGASLWSSC 1150 1160 1170 1180 1190 1200 VVLPLLALTW MSAVLAVTDR RSALFQILFA VFDSLEGFVI VMVHCILRRE VQDAVKCRVV 1210 1220 1230 1240 1250 1260 DRQEEGNGDS GGSFQNGHAQ LMTDFEKDVD LACRSVLNKD IAACRTATIT GTLKRPSLPE 1270 1280 1290 1300 1310 1320 EEKLKLAHAK GPPTNFNSLP ANVSKLHLHG SPRYPGGPLP DFPNHSLTLK RDKAPKSSFV 1330 1340 1350 1360 1370 1380 GDGDIFKKLD SELSRAQEKA LDTSYVILPT ATATLRPKPK EEPKYSIHID QMPQTRLIHL 1390 1400 1410 1420 1430 1440 STAPEASLPA RSPPSRQPPS GGPPEAPPAQ PPPPPPPPPP PPQQPLPPPP NLEPAPPSLG 1450 1460 1470 1480 1490 1500 DPGEPAAHPG PSTGPSTKNE NVATLSVSSL ERRKSRYAEL DFEKIMHTRK RHQDMFQDLN 1510 1520 1530 1540 1550 1560 RKLQHAAEKD KEVLGPDSKP EKQQTPNKRP WESLRKAHGT PTWVKKELEP LQPSPLELRS 1570 1580 VEWERSGATI PLVGQDIIDL QTEV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel brain-specific p53-target gene, BAI1, containing thrombospondin type 1 repeats inhibits experimental angiogenesis." Nishimori H., Shiratsuchi T., Urano T., Kimura Y., Kiyono K., Tatsumi K., Yoshida S., Ono M., Kuwano M., Nakamura Y., Tokino T. Oncogene 15:2145-2150(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal brain.
[2]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Cloning and characterization of BAI-associated protein 1: a PDZ domain-containing protein that interacts with BAI1." Shiratsuchi T., Futamura M., Oda K., Nishimori H., Nakamura Y., Tokino T. Biochem. Biophys. Res. Commun. 247:597-604(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MAGI1.
[4]	"Identification of BAIAP2 (BAI-associated protein 2), a novel human homologue of hamster IRSp53, whose SH3 domain interacts with the cytoplasmic domain of BAI1." Oda K., Shiratsuchi T., Nishimori H., Inazawa J., Yoshikawa H., Taketani Y., Nakamura Y., Tokino T. Cytogenet. Cell Genet. 84:75-82(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH BAIAP2.
[5]	"Interaction of the tumor suppressor PTEN/MMAC with a PDZ domain of MAGI3, a novel membrane-associated guanylate kinase." Wu Y., Dowbenko D., Spencer S., Laura R., Lee J., Gu Q., Lasky L.A. J. Biol. Chem. 275:21477-21485(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MAGI3.
[6]	"Overexpression of the p53-inducible brain-specific angiogenesis inhibitor 1 suppresses efficiently tumour angiogenesis." Duda D.G., Sunamura M., Lozonschi L., Yokoyama T., Yatsuoka T., Motoi F., Horii A., Tani K., Asano S., Nakamura Y., Matsuno S. Br. J. Cancer 86:490-496(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[7]	"Brain-specific angiogenesis inhibitor 1 (BAI1) is expressed in human cerebral neuronal cells." Mori K., Kanemura Y., Fujikawa H., Nakano A., Ikemoto H., Ozaki I., Matsumoto T., Tamura K., Yokota M., Arita N. Neurosci. Res. 43:69-74(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[8]	"Brain angiogenesis inhibitor 1 is differentially expressed in normal brain and glioblastoma independently of p53 expression." Kaur B., Brat D.J., Calkins C.C., Van Meir E.G. Am. J. Pathol. 162:19-27(2003) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[9]	"A novel evolutionarily conserved domain of cell-adhesion GPCRs mediates autoproteolysis." Arac D., Boucard A.A., Bolliger M.F., Nguyen J., Soltis S.M., Sudhof T.C., Brunger A.T. EMBO J. 31:1364-1378(2012) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEOLYTIC PROCESSING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB005297 mRNA. Translation: BAA23647.1. AC139676 Genomic DNA. No translation available.
IPI	IPI00022333.
PIR	T00026.
RefSeq	NP_001693.2. NM_001702.2.
UniGene	Hs.194654.
3D structure databases
ProteinModelPortal	O14514.
ModBase	Search...
Protein-protein interaction databases
IntAct	O14514. 2 interactions.
MINT	MINT-93782.
STRING	9606.ENSP00000313046.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	O14514.
Proteomic databases
PaxDb	O14514.
PRIDE	O14514.
Protocols and materials databases
DNASU	575.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000323289; ENSP00000313046; ENSG00000181790. ENST00000517894; ENSP00000430945; ENSG00000181790.
GeneID	575.
KEGG	hsa:575.
Organism-specific databases
CTD	575.
GeneCards	GC08P143542.
H-InvDB	HIX0025534.
HGNC	HGNC:943. BAI1.
HPA	HPA038785.
MIM	602682. gene.
neXtProt	NX_O14514.
PharmGKB	PA25247.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG285547.
HOGENOM	HOG000230916.
HOVERGEN	HBG004813.
InParanoid	O14514.
KO	K04596.
OMA	DSKPEKQ.
OrthoDB	EOG4SF955.
Gene expression databases
ArrayExpress	O14514.
Bgee	O14514.
CleanEx	HS_BAI1.
Genevestigator	O14514.
GermOnline	ENSG00000181790. Homo sapiens.
Family and domain databases
InterPro	IPR022624. DUF3497. IPR017981. GPCR_2-like. IPR008077. GPCR_2_brain-spec_angio_inhib. IPR001879. GPCR_2_extracellular_dom. IPR000832. GPCR_2_secretin-like. IPR000203. GPS_dom. IPR000884. Thrombospondin_1_rpt. [Graphical view]
Pfam	PF00002. 7tm_2. 1 hit. PF12003. DUF3497. 1 hit. PF01825. GPS. 1 hit. PF02793. HRM. 1 hit. PF00090. TSP_1. 5 hits. [Graphical view]
PRINTS	PR01694. BAIPRECURSOR. PR00249. GPCRSECRETIN.
SMART	SM00303. GPS. 1 hit. SM00008. HormR. 1 hit. SM00209. TSP1. 5 hits. [Graphical view]
SUPFAM	SSF82895. TSP1. 5 hits.
PROSITE	PS00649. G_PROTEIN_RECEP_F2_1. False negative. PS00650. G_PROTEIN_RECEP_F2_2. False negative. PS50227. G_PROTEIN_RECEP_F2_3. 1 hit. PS50261. G_PROTEIN_RECEP_F2_4. 1 hit. PS50221. GPS. 1 hit. PS50092. TSP1. 5 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	575.
NextBio	2345.
SOURCE	Search...

Entry information

Entry name

BAI1_HUMAN

Accession

Primary (citable) accession number: O14514

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	October 31, 2012
Last modified:	April 3, 2013
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.