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O14513 (NCKP5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nck-associated protein 5

Short name=NAP-5
Alternative name(s):
Peripheral clock protein
Gene names
Name:NCKAP5
Synonyms:ERIH, NAP5
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1909 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Interacts with the SH3-containing region of the adapter protein NCK.

Tissue specificity

Expressed in fetal and adult brain, leukocytes and fetal fibroblasts.

Caution

It is uncertain whether Met-1 or Met-26 is the initiator.

Sequence caution

The sequence BAA22433.1 differs from that shown. Reason: Frameshift at positions 856, 877, 920, 926, 957 and 967.

The sequence BAC85920.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainCoiled coil
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O14513-1)

Also known as: Peripheral clock protein 2; ERIH2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O14513-2)

Also known as: Peripheral clock protein 1; ERIH1;

The sequence of this isoform differs from the canonical sequence as follows:
     365-1683: Missing.
Isoform 3 (identifier: O14513-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-25: Missing.
     194-203: AENSALALEN → KHGWNWRPLS
     204-1909: Missing.
Isoform 4 (identifier: O14513-4)

The sequence of this isoform differs from the canonical sequence as follows:
     115-118: MEET → RRKM
     119-1909: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19091909Nck-associated protein 5
PRO_0000096711

Regions

Coiled coil71 – 253183 Potential
Compositional bias331 – 38656Ser-rich
Compositional bias927 – 9337Poly-Pro
Compositional bias1080 – 113051Ser-rich

Amino acid modifications

Modified residue17621Phosphoserine Ref.6
Modified residue17631Phosphoserine Ref.6

Natural variations

Alternative sequence1 – 2525Missing in isoform 3.
VSP_021601
Alternative sequence115 – 1184MEET → RRKM in isoform 4.
VSP_021602
Alternative sequence119 – 19091791Missing in isoform 4.
VSP_021603
Alternative sequence194 – 20310AENSALALEN → KHGWNWRPLS in isoform 3.
VSP_021604
Alternative sequence204 – 19091706Missing in isoform 3.
VSP_021605
Alternative sequence365 – 16831319Missing in isoform 2.
VSP_021606
Natural variant2501R → Q.
Corresponds to variant rs58963837 [ dbSNP | Ensembl ].
VAR_061686
Natural variant6001S → T.
Corresponds to variant rs17325719 [ dbSNP | Ensembl ].
VAR_051220
Natural variant9371V → I.
Corresponds to variant rs12611515 [ dbSNP | Ensembl ].
VAR_051221
Natural variant9771I → T.
Corresponds to variant rs12691830 [ dbSNP | Ensembl ].
VAR_051222
Natural variant10931N → Y.
Corresponds to variant rs16841277 [ dbSNP | Ensembl ].
VAR_051223
Natural variant12601P → Q.
Corresponds to variant rs13016342 [ dbSNP | Ensembl ].
VAR_051224
Natural variant14031V → A.
Corresponds to variant rs2278752 [ dbSNP | Ensembl ].
VAR_051225

Experimental info

Sequence conflict931 – 9322PP → QS in BAA22433. Ref.5
Sequence conflict9481S → P in BAA22433. Ref.5
Sequence conflict10181T → S in BAA22433. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Peripheral clock protein 2) (ERIH2) [UniParc].

Last modified November 28, 2006. Version 2.
Checksum: 74CE34DF3ED05A29

FASTA1,909208,537
        10         20         30         40         50         60 
MEGKRQLEKR DFGKRLSLDS SLVEYMDSNK YIEHLLTQLE EQHRSLWREK LAVARLQREV 

        70         80         90        100        110        120 
AQRTSEGAMH EKLIHELEEE RHLRLQSEKR LQEVTLESER NRIQMRSLQQ QFSRMEETVR 

       130        140        150        160        170        180 
NLLQSQGSPE QKKEETVNIM VYQEKLSEEE RKHKEALEDL HMVVDEDSRS ESSSTDEGKE 

       190        200        210        220        230        240 
KTKLLLERLK ALEAENSALA LENENQREQY ERCLDEVANQ VVQALLTQKD LREECVKLKT 

       250        260        270        280        290        300 
RVFDLEQQNR TLSILFQQRV RPTSDLLLQK LHSRLLDLSS GDLLSEVERN RSLTQSRTDA 

       310        320        330        340        350        360 
EVHEHQLNTK SALKCPGLGA VIPGHLCPRN SYSSSSELSL SSTCSEYSSG SSYTWHDGKN 

       370        380        390        400        410        420 
LRKRQSSQNW DKRLSIDSSL PSGFASPTNE LPPTRIKESH ILEGLRKLQK RKVLLEPPSV 

       430        440        450        460        470        480 
ITKWGYKDCM NSNEGIYSPG IKSSSLKEYP PCKTADLGSP CKEPHKTFVY DLDSHVDADD 

       490        500        510        520        530        540 
DPSTLALLQA VPNQSCRPHG SKLTHSVSDS LFGWETNRKH FLEGTSSVYP KERPEKLTSC 

       550        560        570        580        590        600 
ASSCPLEMKL CPSVQTPQVQ RERGPQGQGH GRMALNLQLS DTDDNETFDE LHIESSDEKS 

       610        620        630        640        650        660 
PSDVSLAADT DKSVENLDVL VGFGKSLCGS PEEEEKQVPI PSETRPKTFS FIKQQRVVKR 

       670        680        690        700        710        720 
TSSEECVTVI FDAEDGEPIE FSSHQTGVVT VTRNEISINS TPAGPKAEHT ELLPQGIACL 

       730        740        750        760        770        780 
QPRAAARDYT FFKRSEEDTE KNIPKDNVDN VPRVSTESFS SRTVTQNPQQ QKLVKPTHNI 

       790        800        810        820        830        840 
SCQSNSRSSA PMGIYQKQNL TKIPPRGKSS PQKSKLMEPE ATTLLPSSGL VTLEKSPALA 

       850        860        870        880        890        900 
PGKLSRFMKT ESSGPLFELR SDPHIPKHSA QLPHSSRMPS RRDWVQCPKS QTPGSRSRPA 

       910        920        930        940        950        960 
IESSDSGEPP TRDEHCGSGP EAGVKSPSPP PPPGRSVSLL ARPSYDYSPA PSSTKSETRV 

       970        980        990       1000       1010       1020 
PSETARTPFK SPLLKGISAP VISSNPATTE VQRKKPSVAF KKPIFTHPMP SPEAVIQTRC 

      1030       1040       1050       1060       1070       1080 
PAHAPSSSFT VMALGPPKVS PKRGVPKTSP RQTLGTPQRD IGLQTPRISP STHEPLEMTS 

      1090       1100       1110       1120       1130       1140 
SKSVSPGRKG QLNDSASTPP KPSFLGVNES PSSQVSSSSS SSSPAKSHNS PHGCQSAHEK 

      1150       1160       1170       1180       1190       1200 
GLKTRLPVGL KVLMKSPQLL RKSSTVPGKH EKDSLNEASK SSVAVNKSKP EDSKNPASME 

      1210       1220       1230       1240       1250       1260 
ITAGERNVTL PDSQAQGSLA DGLPLETALQ EPLESSIPGS DGRDGVDNRS MRRSLSSSKP 

      1270       1280       1290       1300       1310       1320 
HLKPALGMNG AKARSHSFST HSGDKPSTPP IEGSGKVRTQ IITNTAERGN SLTRQNSSTE 

      1330       1340       1350       1360       1370       1380 
SSPNKAPSAP MLESLPSVGR PSGHPSSGKG SLGSSGSFSS QHGSPSKLPL RIPPKSEGLL 

      1390       1400       1410       1420       1430       1440 
IPPGKEDQQA FTQGECPSAN VAVLGEPGSD RRSCPPTPTD CPEALQSPGR TQHPSTFETS 

      1450       1460       1470       1480       1490       1500 
STSKLETSGR HPDASATATD AVSSEAPLSP TIEEKVMLCI QENVEKGQVQ TKPTSVEAKQ 

      1510       1520       1530       1540       1550       1560 
KPGPSFASWF GFRKSRLPAL SSRKMDISKT KVEKKDAKVL GFGNRQLKSE RKKEKKKPEL 

      1570       1580       1590       1600       1610       1620 
QCETENELIK DTKSADNPDG GLQSKNNRRT PQDIYNQLKI EPRNRHSPVA CSTKDTFMTE 

      1630       1640       1650       1660       1670       1680 
LLNRVDKKAA PQTESGSSNA SCRNVLKGSS QGSCLIGSSI STQGNHKKNM KIKADMEVPK 

      1690       1700       1710       1720       1730       1740 
DSLVKEANEN LQEDEDDAVA DSVFQSHIIE SNCQMRTLDS GIGTFPLPDS GNRSTGRYLC 

      1750       1760       1770       1780       1790       1800 
QPDSPEDAEP LLPLQSALSA VSSMRAQTLE REVPSSTDGQ RPADSAIVHS TSDPIMTARG 

      1810       1820       1830       1840       1850       1860 
MRPLQSRLPK PASSGKVSSQ KQNEAEPRPQ TCSSFGYAED PMASQPLPDW GSEVAATGTQ 

      1870       1880       1890       1900 
DKAPRMCTYS ASGGSNSDSD LDYGDNGFGA GRGQLVKALK SAAPEIETT 

« Hide

Isoform 2 (Peripheral clock protein 1) (ERIH1) [UniParc].

Checksum: 672897BBC6BF2B1B
Show »

FASTA59066,362
Isoform 3 [UniParc].

Checksum: 1E1B278017DEE1C4
Show »

FASTA17821,367
Isoform 4 [UniParc].

Checksum: 7BBEAADEDE21F27A
Show »

FASTA11814,443

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1586-1909 (ISOFORM 1).
Tissue: Amygdala and Teratocarcinoma.
[2]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 6-1909 (ISOFORM 4).
Tissue: Testis.
[4]Hisa T.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 69-1909 (ISOFORMS 1 AND 2).
[5]"A novel ligand for an SH3 domain of the adaptor protein Nck bears an SH2 domain and nuclear signaling motifs."
Matuoka K., Miki H., Takahashi K., Takenawa T.
Biochem. Biophys. Res. Commun. 239:488-492(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 661-1077 (ISOFORM 1).
Tissue: Brain.
[6]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1762 AND SER-1763, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK057980 mRNA. No translation available.
AK092189 mRNA. Translation: BAC03824.1.
AK124659 mRNA. Translation: BAC85920.1. Different initiation.
AC010890 Genomic DNA. No translation available.
AC010974 Genomic DNA. No translation available.
AC011243 Genomic DNA. No translation available.
AC011755 Genomic DNA. No translation available.
AC011996 Genomic DNA. No translation available.
AC012489 Genomic DNA. No translation available.
AC016909 Genomic DNA. No translation available.
BC110831 mRNA. Translation: AAI10832.1.
AY946007 mRNA. Translation: AAY23346.1.
AY946008 mRNA. Translation: AAY23347.1.
AB005217 mRNA. Translation: BAA22433.1. Frameshift.
CCDSCCDS46417.1. [O14513-2]
CCDS46418.1. [O14513-1]
RefSeqNP_997246.2. NM_207363.2. [O14513-1]
NP_997364.3. NM_207481.3. [O14513-2]
UniGeneHs.537329.

3D structure databases

ProteinModelPortalO14513.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid131288. 4 interactions.
IntActO14513. 7 interactions.
MINTMINT-7241081.
STRING9606.ENSP00000387128.

PTM databases

PhosphoSiteO14513.

Proteomic databases

PaxDbO14513.
PRIDEO14513.

Protocols and materials databases

DNASU344148.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000317721; ENSP00000380603; ENSG00000176771. [O14513-1]
ENST00000405974; ENSP00000385692; ENSG00000176771. [O14513-2]
ENST00000409213; ENSP00000386952; ENSG00000176771. [O14513-2]
ENST00000409261; ENSP00000387128; ENSG00000176771. [O14513-1]
GeneID344148.
KEGGhsa:344148.
UCSCuc002ttp.3. human. [O14513-1]
uc002ttq.3. human. [O14513-2]
uc002tts.1. human. [O14513-3]
uc002ttt.1. human. [O14513-4]

Organism-specific databases

CTD344148.
GeneCardsGC02M133429.
H-InvDBHIX0021656.
HGNCHGNC:29847. NCKAP5.
HPAHPA034639.
HPA044859.
MIM608789. gene.
neXtProtNX_O14513.
PharmGKBPA165696957.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG85857.
HOVERGENHBG062295.
InParanoidO14513.
OMADSLFGWE.
OrthoDBEOG7PGDQM.
PhylomeDBO14513.
TreeFamTF331208.

Gene expression databases

ArrayExpressO14513.
BgeeO14513.
GenevestigatorO14513.

Family and domain databases

InterProIPR026163. Nckap5l.
[Graphical view]
PANTHERPTHR21740. PTHR21740. 1 hit.
ProtoNetSearch...

Other

ChiTaRSNCKAP5. human.
GenomeRNAi344148.
NextBio98641.
PROO14513.
SOURCESearch...

Entry information

Entry nameNCKP5_HUMAN
AccessionPrimary (citable) accession number: O14513
Secondary accession number(s): B8ZZL0 expand/collapse secondary AC list , Q29SS9, Q29ST0, Q2NL90, Q6ZVE2, Q8NAS3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: November 28, 2006
Last modified: July 9, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM