O14513 (NCKP5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nck-associated protein 5
Short name=NAP-5

Alternative name(s):
Peripheral clock protein

Gene names

Name:	NCKAP5
Synonyms:	ERIH, NAP5

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1909 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subunit structure	Interacts with the SH3-containing region of the adapter protein Nck.
Tissue specificity	Expressed in fetal and adult brain, leukocytes and fetal fibroblasts.
Caution	It is uncertain whether Met-1 or Met-26 is the initiator.
Sequence caution	The sequence BAA22433.1 differs from that shown. Reason: Frameshift at positions 856, 877, 920, 926, 957 and 967. The sequence BAC85920.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O14513-1)

Also known as: Peripheral clock protein 2; ERIH2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O14513-2)

Also known as: Peripheral clock protein 1; ERIH1;

The sequence of this isoform differs from the canonical sequence as follows:
     365-1683: Missing.

Isoform 3 (identifier: O14513-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-25: Missing.
     194-203: AENSALALEN → KHGWNWRPLS
     204-1909: Missing.

Isoform 4 (identifier: O14513-4)

The sequence of this isoform differs from the canonical sequence as follows:
     115-118: MEET → RRKM
     119-1909: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1909	1909	Nck-associated protein 5		PRO_0000096711
Regions
	Coiled coil	71 – 253	183	Potential
	Compositional bias	331 – 386	56	Ser-rich
	Compositional bias	927 – 933	7	Poly-Pro
	Compositional bias	1080 – 1130	51	Ser-rich
Natural variations
	Alternative sequence	1 – 25	25	Missing in isoform 3.		VSP_021601
	Alternative sequence	115 – 118	4	MEET → RRKM in isoform 4.		VSP_021602
	Alternative sequence	119 – 1909	1791	Missing in isoform 4.		VSP_021603
	Alternative sequence	194 – 203	10	AENSALALEN → KHGWNWRPLS in isoform 3.		VSP_021604
	Alternative sequence	204 – 1909	1706	Missing in isoform 3.		VSP_021605
	Alternative sequence	365 – 1683	1319	Missing in isoform 2.		VSP_021606
	Natural variant	250	1	R → Q. Corresponds to variant rs58963837 [ dbSNP | Ensembl ].		VAR_061686
	Natural variant	600	1	S → T. Corresponds to variant rs17325719 [ dbSNP | Ensembl ].		VAR_051220
	Natural variant	937	1	V → I. Corresponds to variant rs12611515 [ dbSNP | Ensembl ].		VAR_051221
	Natural variant	977	1	I → T. Corresponds to variant rs12691830 [ dbSNP | Ensembl ].		VAR_051222
	Natural variant	1093	1	N → Y. Corresponds to variant rs16841277 [ dbSNP | Ensembl ].		VAR_051223
	Natural variant	1260	1	P → Q. Corresponds to variant rs13016342 [ dbSNP | Ensembl ].		VAR_051224
	Natural variant	1403	1	V → A. Corresponds to variant rs2278752 [ dbSNP | Ensembl ].		VAR_051225
Experimental info
	Sequence conflict	931 – 932	2	PP → QS in BAA22433. Ref.5
	Sequence conflict	948	1	S → P in BAA22433. Ref.5
	Sequence conflict	1018	1	T → S in BAA22433. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Peripheral clock protein 2) (ERIH2) [UniParc]. Last modified November 28, 2006. Version 2. Checksum: 74CE34DF3ED05A29	FASTA	1,909	208,537
	10 20 30 40 50 60 MEGKRQLEKR DFGKRLSLDS SLVEYMDSNK YIEHLLTQLE EQHRSLWREK LAVARLQREV 70 80 90 100 110 120 AQRTSEGAMH EKLIHELEEE RHLRLQSEKR LQEVTLESER NRIQMRSLQQ QFSRMEETVR 130 140 150 160 170 180 NLLQSQGSPE QKKEETVNIM VYQEKLSEEE RKHKEALEDL HMVVDEDSRS ESSSTDEGKE 190 200 210 220 230 240 KTKLLLERLK ALEAENSALA LENENQREQY ERCLDEVANQ VVQALLTQKD LREECVKLKT 250 260 270 280 290 300 RVFDLEQQNR TLSILFQQRV RPTSDLLLQK LHSRLLDLSS GDLLSEVERN RSLTQSRTDA 310 320 330 340 350 360 EVHEHQLNTK SALKCPGLGA VIPGHLCPRN SYSSSSELSL SSTCSEYSSG SSYTWHDGKN 370 380 390 400 410 420 LRKRQSSQNW DKRLSIDSSL PSGFASPTNE LPPTRIKESH ILEGLRKLQK RKVLLEPPSV 430 440 450 460 470 480 ITKWGYKDCM NSNEGIYSPG IKSSSLKEYP PCKTADLGSP CKEPHKTFVY DLDSHVDADD 490 500 510 520 530 540 DPSTLALLQA VPNQSCRPHG SKLTHSVSDS LFGWETNRKH FLEGTSSVYP KERPEKLTSC 550 560 570 580 590 600 ASSCPLEMKL CPSVQTPQVQ RERGPQGQGH GRMALNLQLS DTDDNETFDE LHIESSDEKS 610 620 630 640 650 660 PSDVSLAADT DKSVENLDVL VGFGKSLCGS PEEEEKQVPI PSETRPKTFS FIKQQRVVKR 670 680 690 700 710 720 TSSEECVTVI FDAEDGEPIE FSSHQTGVVT VTRNEISINS TPAGPKAEHT ELLPQGIACL 730 740 750 760 770 780 QPRAAARDYT FFKRSEEDTE KNIPKDNVDN VPRVSTESFS SRTVTQNPQQ QKLVKPTHNI 790 800 810 820 830 840 SCQSNSRSSA PMGIYQKQNL TKIPPRGKSS PQKSKLMEPE ATTLLPSSGL VTLEKSPALA 850 860 870 880 890 900 PGKLSRFMKT ESSGPLFELR SDPHIPKHSA QLPHSSRMPS RRDWVQCPKS QTPGSRSRPA 910 920 930 940 950 960 IESSDSGEPP TRDEHCGSGP EAGVKSPSPP PPPGRSVSLL ARPSYDYSPA PSSTKSETRV 970 980 990 1000 1010 1020 PSETARTPFK SPLLKGISAP VISSNPATTE VQRKKPSVAF KKPIFTHPMP SPEAVIQTRC 1030 1040 1050 1060 1070 1080 PAHAPSSSFT VMALGPPKVS PKRGVPKTSP RQTLGTPQRD IGLQTPRISP STHEPLEMTS 1090 1100 1110 1120 1130 1140 SKSVSPGRKG QLNDSASTPP KPSFLGVNES PSSQVSSSSS SSSPAKSHNS PHGCQSAHEK 1150 1160 1170 1180 1190 1200 GLKTRLPVGL KVLMKSPQLL RKSSTVPGKH EKDSLNEASK SSVAVNKSKP EDSKNPASME 1210 1220 1230 1240 1250 1260 ITAGERNVTL PDSQAQGSLA DGLPLETALQ EPLESSIPGS DGRDGVDNRS MRRSLSSSKP 1270 1280 1290 1300 1310 1320 HLKPALGMNG AKARSHSFST HSGDKPSTPP IEGSGKVRTQ IITNTAERGN SLTRQNSSTE 1330 1340 1350 1360 1370 1380 SSPNKAPSAP MLESLPSVGR PSGHPSSGKG SLGSSGSFSS QHGSPSKLPL RIPPKSEGLL 1390 1400 1410 1420 1430 1440 IPPGKEDQQA FTQGECPSAN VAVLGEPGSD RRSCPPTPTD CPEALQSPGR TQHPSTFETS 1450 1460 1470 1480 1490 1500 STSKLETSGR HPDASATATD AVSSEAPLSP TIEEKVMLCI QENVEKGQVQ TKPTSVEAKQ 1510 1520 1530 1540 1550 1560 KPGPSFASWF GFRKSRLPAL SSRKMDISKT KVEKKDAKVL GFGNRQLKSE RKKEKKKPEL 1570 1580 1590 1600 1610 1620 QCETENELIK DTKSADNPDG GLQSKNNRRT PQDIYNQLKI EPRNRHSPVA CSTKDTFMTE 1630 1640 1650 1660 1670 1680 LLNRVDKKAA PQTESGSSNA SCRNVLKGSS QGSCLIGSSI STQGNHKKNM KIKADMEVPK 1690 1700 1710 1720 1730 1740 DSLVKEANEN LQEDEDDAVA DSVFQSHIIE SNCQMRTLDS GIGTFPLPDS GNRSTGRYLC 1750 1760 1770 1780 1790 1800 QPDSPEDAEP LLPLQSALSA VSSMRAQTLE REVPSSTDGQ RPADSAIVHS TSDPIMTARG 1810 1820 1830 1840 1850 1860 MRPLQSRLPK PASSGKVSSQ KQNEAEPRPQ TCSSFGYAED PMASQPLPDW GSEVAATGTQ 1870 1880 1890 1900 DKAPRMCTYS ASGGSNSDSD LDYGDNGFGA GRGQLVKALK SAAPEIETT
	Isoform 2 (Peripheral clock protein 1) (ERIH1) [UniParc]. Checksum: 672897BBC6BF2B1B	FASTA	590	66,362
	10 20 30 40 50 60 MEGKRQLEKR DFGKRLSLDS SLVEYMDSNK YIEHLLTQLE EQHRSLWREK LAVARLQREV 70 80 90 100 110 120 AQRTSEGAMH EKLIHELEEE RHLRLQSEKR LQEVTLESER NRIQMRSLQQ QFSRMEETVR 130 140 150 160 170 180 NLLQSQGSPE QKKEETVNIM VYQEKLSEEE RKHKEALEDL HMVVDEDSRS ESSSTDEGKE 190 200 210 220 230 240 KTKLLLERLK ALEAENSALA LENENQREQY ERCLDEVANQ VVQALLTQKD LREECVKLKT 250 260 270 280 290 300 RVFDLEQQNR TLSILFQQRV RPTSDLLLQK LHSRLLDLSS GDLLSEVERN RSLTQSRTDA 310 320 330 340 350 360 EVHEHQLNTK SALKCPGLGA VIPGHLCPRN SYSSSSELSL SSTCSEYSSG SSYTWHDGKN 370 380 390 400 410 420 LRKRVKEANE NLQEDEDDAV ADSVFQSHII ESNCQMRTLD SGIGTFPLPD SGNRSTGRYL 430 440 450 460 470 480 CQPDSPEDAE PLLPLQSALS AVSSMRAQTL EREVPSSTDG QRPADSAIVH STSDPIMTAR 490 500 510 520 530 540 GMRPLQSRLP KPASSGKVSS QKQNEAEPRP QTCSSFGYAE DPMASQPLPD WGSEVAATGT 550 560 570 580 590 QDKAPRMCTY SASGGSNSDS DLDYGDNGFG AGRGQLVKAL KSAAPEIETT
	Isoform 3 [UniParc]. Checksum: 1E1B278017DEE1C4	FASTA	178	21,367
	10 20 30 40 50 60 MDSNKYIEHL LTQLEEQHRS LWREKLAVAR LQREVAQRTS EGAMHEKLIH ELEEERHLRL 70 80 90 100 110 120 QSEKRLQEVT LESERNRIQM RSLQQQFSRM EETVRNLLQS QGSPEQKKEE TVNIMVYQEK 130 140 150 160 170 LSEEERKHKE ALEDLHMVVD EDSRSESSST DEGKEKTKLL LERLKALEKH GWNWRPLS
	Isoform 4 [UniParc]. Checksum: 7BBEAADEDE21F27A	FASTA	118	14,443
	10 20 30 40 50 60 MEGKRQLEKR DFGKRLSLDS SLVEYMDSNK YIEHLLTQLE EQHRSLWREK LAVARLQREV 70 80 90 100 110 AQRTSEGAMH EKLIHELEEE RHLRLQSEKR LQEVTLESER NRIQMRSLQQ QFSRRRKM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1586-1909 (ISOFORM 1). Tissue: Amygdala and Teratocarcinoma.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 6-1909 (ISOFORM 4). Tissue: Testis.
[4]	Hisa T. Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 69-1909 (ISOFORMS 1 AND 2).
[5]	"A novel ligand for an SH3 domain of the adaptor protein Nck bears an SH2 domain and nuclear signaling motifs." Matuoka K., Miki H., Takahashi K., Takenawa T. Biochem. Biophys. Res. Commun. 239:488-492(1997) [PubMed: 9344857] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 661-1077 (ISOFORM 1). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK057980 mRNA. No translation available. AK092189 mRNA. Translation: BAC03824.1. AK124659 mRNA. Translation: BAC85920.1. Different initiation. AC010890 Genomic DNA. No translation available. AC010974 Genomic DNA. No translation available. AC011243 Genomic DNA. No translation available. AC011755 Genomic DNA. No translation available. AC011996 Genomic DNA. No translation available. AC012489 Genomic DNA. No translation available. AC016909 Genomic DNA. No translation available. BC110831 mRNA. Translation: AAI10832.1. AY946007 mRNA. Translation: AAY23346.1. AY946008 mRNA. Translation: AAY23347.1. AB005217 mRNA. Translation: BAA22433.1. Frameshift.
IPI	IPI00410416. IPI00419253. IPI00807489. IPI00807540.
RefSeq	NP_997246.2. NM_207363.2. NP_997364.3. NM_207481.3.
UniGene	Hs.537329.
3D structure databases
ProteinModelPortal	O14513.
ModBase	Search...
Protein-protein interaction databases
IntAct	O14513. 6 interactions.
MINT	MINT-7241081.
STRING	O14513.
PTM databases
PhosphoSite	O14513.
Proteomic databases
PRIDE	O14513.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000409261; ENSP00000387128; ENSG00000176771.
GeneID	344148.
KEGG	hsa:344148.
UCSC	uc002ttp.1. human. uc002ttq.1. human. uc002tts.1. human. uc002ttt.1. human.
Organism-specific databases
CTD	344148.
GeneCards	GC02M133429.
HGNC	HGNC:29847. NCKAP5.
HPA	HPA034639.
MIM	608789. gene.
neXtProt	NX_O14513.
PharmGKB	PA165696957.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG04399.
GeneTree	ENSGT00530000063607.
HOGENOM	HBG283756.
HOVERGEN	HBG062295.
InParanoid	O14513.
OMA	PGKHEKD.
OrthoDB	EOG43JC66.
Gene expression databases
ArrayExpress	O14513.
Bgee	O14513.
Genevestigator	O14513.
Family and domain databases
ProtoNet	Search...
Other
NextBio	98641.
SOURCE	Search...

Entry information

Entry name

NCKP5_HUMAN

Accession

Primary (citable) accession number: O14513
Secondary accession number(s): B8ZZL0 , Q29SS9, Q29ST0, Q2NL90, Q6ZVE2, Q8NAS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	November 28, 2006
Last modified:	January 25, 2012
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.