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Protein

Phospholipid phosphatase 3

Gene

PLPP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). In addition it hydrolyzes lysophosphatidic acid (LPA), ceramide-1-phosphate (C-1-P) and sphingosine-1-phosphate (S-1-P). The relative catalytic efficiency is LPA = PA > C-1-P > S-1-P. May be involved in cell adhesion and in cell-cell interactions.

Catalytic activityi

A 1,2-diacylglycerol 3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate.

Enzyme regulationi

Inhibited by sphingosine, zinc ions and propanolol. Not inhibited by N-ethylmaleimide treatment.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciZFISH:HS08670-MONOMER.
ReactomeiR-HSA-1660661. Sphingolipid de novo biosynthesis.
SignaLinkiO14495.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipid phosphatase 3Imported (EC:3.1.3.4)
Alternative name(s):
Lipid phosphate phosphohydrolase 3
PAP2-beta
Phosphatidate phosphohydrolase type 2b
Phosphatidic acid phosphatase 2b
Short name:
PAP-2b
Short name:
PAP2b
Vascular endothelial growth factor and type I collagen-inducible protein
Short name:
VCIP
Gene namesi
Name:PLPP3Imported
Synonyms:LPP3, PPAP2B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:9229. PLPP3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 33CytoplasmicSequence analysisAdd BLAST33
Transmembranei34 – 54HelicalSequence analysisAdd BLAST21
Topological domaini55 – 85LumenalSequence analysisAdd BLAST31
Transmembranei86 – 106HelicalSequence analysisAdd BLAST21
Topological domaini107 – 122CytoplasmicSequence analysisAdd BLAST16
Transmembranei123 – 143HelicalSequence analysisAdd BLAST21
Topological domaini144 – 193LumenalSequence analysisAdd BLAST50
Transmembranei194 – 214HelicalSequence analysisAdd BLAST21
Topological domaini215 – 227CytoplasmicSequence analysisAdd BLAST13
Transmembranei228 – 248HelicalSequence analysisAdd BLAST21
Topological domaini249 – 257LumenalSequence analysis9
Transmembranei258 – 278HelicalSequence analysisAdd BLAST21
Topological domaini279 – 311CytoplasmicSequence analysisAdd BLAST33

GO - Cellular componenti

  • adherens junction Source: BHF-UCL
  • extracellular exosome Source: UniProtKB
  • Golgi apparatus Source: HPA
  • integral component of plasma membrane Source: GO_Central
  • membrane Source: ProtInc
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi8613.
OpenTargetsiENSG00000162407.
PharmGKBiPA33553.

Polymorphism and mutation databases

BioMutaiPPAP2B.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002209121 – 311Phospholipid phosphatase 3Add BLAST311

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19PhosphoserineCombined sources1
Glycosylationi170N-linked (GlcNAc...)Sequence analysis1
Modified residuei297PhosphoserineCombined sources1

Post-translational modificationi

N-glycosylated. Contains high-mannose oligosaccharides.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiO14495.
PaxDbiO14495.
PeptideAtlasiO14495.
PRIDEiO14495.

PTM databases

DEPODiO14495.
iPTMnetiO14495.
PhosphoSitePlusiO14495.

Expressioni

Tissue specificityi

Ubiquitously expressed. Highly expressed in heart and placenta.

Inductioni

By EGF, VEGF, FGF2 and phorbol myristate acetate (PMA).1 Publication

Gene expression databases

BgeeiENSG00000162407.
CleanExiHS_PPAP2B.
GenevisibleiO14495. HS.

Organism-specific databases

HPAiHPA028892.
HPA072751.

Interactioni

Subunit structurei

Homodimer. This complex seems not to be involved in substrate recognition, it may confer only structural or functional stability.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CTNND1O607169EBI-766232,EBI-701927

Protein-protein interaction databases

BioGridi114171. 5 interactors.
IntActiO14495. 4 interactors.
MINTiMINT-4648964.
STRINGi9606.ENSP00000360296.

Structurei

3D structure databases

ProteinModelPortaliO14495.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3030. Eukaryota.
COG0671. LUCA.
GeneTreeiENSGT00620000087654.
HOGENOMiHOG000041307.
HOVERGENiHBG002048.
InParanoidiO14495.
KOiK01080.
OMAiNNNCKDH.
OrthoDBiEOG091G0K5H.
PhylomeDBiO14495.
TreeFamiTF316040.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR028675. LPP3.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PANTHERiPTHR10165:SF79. PTHR10165:SF79. 1 hit.
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.

Sequencei

Sequence statusi: Complete.

O14495-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNYKYDKAI VPESKNGGSP ALNNNPRRSG SKRVLLICLD LFCLFMAGLP
60 70 80 90 100
FLIIETSTIK PYHRGFYCND ESIKYPLKTG ETINDAVLCA VGIVIAILAI
110 120 130 140 150
ITGEFYRIYY LKKSRSTIQN PYVAALYKQV GCFLFGCAIS QSFTDIAKVS
160 170 180 190 200
IGRLRPHFLS VCNPDFSQIN CSEGYIQNYR CRGDDSKVQE ARKSFFSGHA
210 220 230 240 250
SFSMYTMLYL VLYLQARFTW RGARLLRPLL QFTLIMMAFY TGLSRVSDHK
260 270 280 290 300
HHPSDVLAGF AQGALVACCI VFFVSDLFKT KTTLSLPAPA IRKEILSPVD
310
IIDRNNHHNM M
Length:311
Mass (Da):35,116
Last modified:January 1, 1998 - v1
Checksum:iCB3F60189044DA31
GO

Sequence cautioni

The sequence AAB50222 differs from that shown. Reason: Frameshift at position 225.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32K → M in AAV38396 (Ref. 7) Curated1
Sequence conflicti282T → M in AAH09196 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000889 mRNA. Translation: BAA22594.1.
AF017786 mRNA. Translation: AAC63433.1.
AF480883 mRNA. Translation: AAO84481.1.
AF043329 mRNA. Translation: AAD02271.1.
U79294 mRNA. Translation: AAB50222.1. Frameshift.
AK312439 mRNA. Translation: BAG35348.1.
BT019589 mRNA. Translation: AAV38396.1.
CH471059 Genomic DNA. Translation: EAX06651.1.
CH471059 Genomic DNA. Translation: EAX06652.1.
CH471059 Genomic DNA. Translation: EAX06653.1.
BC009196 mRNA. Translation: AAH09196.1.
CCDSiCCDS604.1.
RefSeqiNP_003704.3. NM_003713.4.
UniGeneiHs.405156.
Hs.619002.

Genome annotation databases

EnsembliENST00000371250; ENSP00000360296; ENSG00000162407.
GeneIDi8613.
KEGGihsa:8613.
UCSCiuc001cyj.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000889 mRNA. Translation: BAA22594.1.
AF017786 mRNA. Translation: AAC63433.1.
AF480883 mRNA. Translation: AAO84481.1.
AF043329 mRNA. Translation: AAD02271.1.
U79294 mRNA. Translation: AAB50222.1. Frameshift.
AK312439 mRNA. Translation: BAG35348.1.
BT019589 mRNA. Translation: AAV38396.1.
CH471059 Genomic DNA. Translation: EAX06651.1.
CH471059 Genomic DNA. Translation: EAX06652.1.
CH471059 Genomic DNA. Translation: EAX06653.1.
BC009196 mRNA. Translation: AAH09196.1.
CCDSiCCDS604.1.
RefSeqiNP_003704.3. NM_003713.4.
UniGeneiHs.405156.
Hs.619002.

3D structure databases

ProteinModelPortaliO14495.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114171. 5 interactors.
IntActiO14495. 4 interactors.
MINTiMINT-4648964.
STRINGi9606.ENSP00000360296.

PTM databases

DEPODiO14495.
iPTMnetiO14495.
PhosphoSitePlusiO14495.

Polymorphism and mutation databases

BioMutaiPPAP2B.

Proteomic databases

MaxQBiO14495.
PaxDbiO14495.
PeptideAtlasiO14495.
PRIDEiO14495.

Protocols and materials databases

DNASUi8613.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371250; ENSP00000360296; ENSG00000162407.
GeneIDi8613.
KEGGihsa:8613.
UCSCiuc001cyj.3. human.

Organism-specific databases

CTDi8613.
DisGeNETi8613.
GeneCardsiPPAP2B.
HGNCiHGNC:9229. PLPP3.
HPAiHPA028892.
HPA072751.
MIMi607125. gene.
neXtProtiNX_O14495.
OpenTargetsiENSG00000162407.
PharmGKBiPA33553.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3030. Eukaryota.
COG0671. LUCA.
GeneTreeiENSGT00620000087654.
HOGENOMiHOG000041307.
HOVERGENiHBG002048.
InParanoidiO14495.
KOiK01080.
OMAiNNNCKDH.
OrthoDBiEOG091G0K5H.
PhylomeDBiO14495.
TreeFamiTF316040.

Enzyme and pathway databases

BioCyciZFISH:HS08670-MONOMER.
ReactomeiR-HSA-1660661. Sphingolipid de novo biosynthesis.
SignaLinkiO14495.

Miscellaneous databases

ChiTaRSiPPAP2B. human.
GeneWikiiPPAP2B.
GenomeRNAii8613.
PROiO14495.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000162407.
CleanExiHS_PPAP2B.
GenevisibleiO14495. HS.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR028675. LPP3.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PANTHERiPTHR10165:SF79. PTHR10165:SF79. 1 hit.
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPLPP3_HUMAN
AccessioniPrimary (citable) accession number: O14495
Secondary accession number(s): B2R651
, D3DQ52, Q5U0F7, Q96GW0, Q99782
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.