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Protein

Multiprotein-bridging factor 1

Gene

MBF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional coactivator that stimulates GCN4-dependent transcriptional activity by bridging the DNA-binding region of GCN4 and TBP (SPT15), thereby recruiting TBP to GCN4-bound promoters.1 Publication

Miscellaneous

Present with 47277 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi96 – 115H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • sequence-specific DNA binding Source: InterPro
  • transcription coactivator activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-33879-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Multiprotein-bridging factor 1
Alternative name(s):
Suppressor of frameshift mutations protein 13
Gene namesi
Name:MBF1
Synonyms:SUF13
Ordered Locus Names:YOR298C-A
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR298C-A
SGDiS000007253 MBF1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi112D → A: Reduces interaction to TBP. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001498141 – 151Multiprotein-bridging factor 1Add BLAST151

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei143PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO14467
PaxDbiO14467
PRIDEiO14467
TopDownProteomicsiO14467

PTM databases

iPTMnetiO14467

Interactioni

Subunit structurei

Interacts with TBP and the transcription factor GCN4.1 Publication

Protein-protein interaction databases

BioGridi34687, 199 interactors
DIPiDIP-8708N
IntActiO14467, 3 interactors
MINTiO14467
STRINGi4932.YOR298C-A

Structurei

3D structure databases

ProteinModelPortaliO14467
SMRiO14467
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini85 – 139HTH cro/C1-typePROSITE-ProRule annotationAdd BLAST55

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni41 – 119Essential for TBP-bindingAdd BLAST79

Sequence similaritiesi

Belongs to the MBF1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000008519
HOGENOMiHOG000195726
InParanoidiO14467
KOiK03627
OMAiIADYECG
OrthoDBiEOG092C5XAS

Family and domain databases

CDDicd00093 HTH_XRE, 1 hit
InterProiView protein in InterPro
IPR001387 Cro/C1-type_HTH
IPR010982 Lambda_DNA-bd_dom_sf
IPR013729 MBF1_N
PfamiView protein in Pfam
PF01381 HTH_3, 1 hit
PF08523 MBF1, 1 hit
SMARTiView protein in SMART
SM00530 HTH_XRE, 1 hit
SUPFAMiSSF47413 SSF47413, 1 hit
PROSITEiView protein in PROSITE
PS50943 HTH_CROC1, 1 hit

Sequencei

Sequence statusi: Complete.

O14467-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDWDTNTII GSRARAGGSG PRANVARSQG QINAARRQGL VVSVDKKYGS
60 70 80 90 100
TNTRGDNEGQ RLTKVDRETD IVKPKKLDPN VGRAISRART DKKMSQKDLA
110 120 130 140 150
TKINEKPTVV NDYEAARAIP NQQVLSKLER ALGVKLRGNN IGSPLGAPKK

K
Length:151
Mass (Da):16,404
Last modified:May 1, 1999 - v2
Checksum:iE687ED8768ACD1DF
GO

Sequence cautioni

The sequence CAA99527 differs from that shown. Reason: Frameshift at position 20.Curated
The sequence CAA99530 differs from that shown. Reason: Frameshift at position 20.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017593 Genomic DNA Translation: BAA33217.1
Z75206 Genomic DNA Translation: CAA99527.1 Frameshift.
Z75207 Genomic DNA Translation: CAA99530.1 Frameshift.
AY260886 Genomic DNA Translation: AAP21754.1
AY692753 Genomic DNA Translation: AAT92772.1
BK006948 Genomic DNA Translation: DAA11064.1
PIRiS72394
RefSeqiNP_014942.4, NM_001184346.3

Genome annotation databases

EnsemblFungiiYOR298C-A; YOR298C-A; YOR298C-A
GeneIDi854474
KEGGisce:YOR298C-A

Similar proteinsi

Entry informationi

Entry nameiMBF1_YEAST
AccessioniPrimary (citable) accession number: O14467
Secondary accession number(s): D6W2Z8, Q7LGJ8, Q86ZS7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: May 1, 1999
Last modified: May 23, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

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