Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Multiprotein-bridging factor 1

Gene

MBF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional coactivator that stimulates GCN4-dependent transcriptional activity by bridging the DNA-binding region of GCN4 and TBP (SPT15), thereby recruiting TBP to GCN4-bound promoters.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi96 – 115H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • sequence-specific DNA binding Source: InterPro
  • transcription coactivator activity Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33879-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Multiprotein-bridging factor 1
Alternative name(s):
Suppressor of frameshift mutations protein 13
Gene namesi
Name:MBF1
Synonyms:SUF13
Ordered Locus Names:YOR298C-A
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR298C-A.
SGDiS000007253. MBF1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi112D → A: Reduces interaction to TBP. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001498141 – 151Multiprotein-bridging factor 1Add BLAST151

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei143PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO14467.
PRIDEiO14467.
TopDownProteomicsiO14467.

PTM databases

iPTMnetiO14467.

Interactioni

Subunit structurei

Interacts with TBP and the transcription factor GCN4.1 Publication

Protein-protein interaction databases

BioGridi34687. 73 interactors.
DIPiDIP-8708N.
IntActiO14467. 2 interactors.
MINTiMINT-8285541.

Structurei

3D structure databases

ProteinModelPortaliO14467.
SMRiO14467.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini85 – 139HTH cro/C1-typePROSITE-ProRule annotationAdd BLAST55

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni41 – 119Essential for TBP-bindingAdd BLAST79

Sequence similaritiesi

Belongs to the MBF1 family.Curated
Contains 1 HTH cro/C1-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000008519.
HOGENOMiHOG000195726.
InParanoidiO14467.
KOiK03627.
OMAiEPKMTQK.
OrthoDBiEOG092C5XAS.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
InterProiIPR001387. Cro/C1-type_HTH.
IPR010982. Lambda_DNA-bd_dom.
IPR013729. MBF1_N.
[Graphical view]
PfamiPF01381. HTH_3. 1 hit.
PF08523. MBF1. 1 hit.
[Graphical view]
SMARTiSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
PROSITEiPS50943. HTH_CROC1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O14467-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDWDTNTII GSRARAGGSG PRANVARSQG QINAARRQGL VVSVDKKYGS
60 70 80 90 100
TNTRGDNEGQ RLTKVDRETD IVKPKKLDPN VGRAISRART DKKMSQKDLA
110 120 130 140 150
TKINEKPTVV NDYEAARAIP NQQVLSKLER ALGVKLRGNN IGSPLGAPKK

K
Length:151
Mass (Da):16,404
Last modified:May 1, 1999 - v2
Checksum:iE687ED8768ACD1DF
GO

Sequence cautioni

The sequence CAA99527 differs from that shown. Reason: Frameshift at position 20.Curated
The sequence CAA99530 differs from that shown. Reason: Frameshift at position 20.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017593 Genomic DNA. Translation: BAA33217.1.
Z75206 Genomic DNA. Translation: CAA99527.1. Frameshift.
Z75207 Genomic DNA. Translation: CAA99530.1. Frameshift.
AY260886 Genomic DNA. Translation: AAP21754.1.
AY692753 Genomic DNA. Translation: AAT92772.1.
BK006948 Genomic DNA. Translation: DAA11064.1.
PIRiS72394.
RefSeqiNP_014942.4. NM_001184346.3.

Genome annotation databases

EnsemblFungiiYOR298C-A; YOR298C-A; YOR298C-A.
GeneIDi854474.
KEGGisce:YOR298C-A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017593 Genomic DNA. Translation: BAA33217.1.
Z75206 Genomic DNA. Translation: CAA99527.1. Frameshift.
Z75207 Genomic DNA. Translation: CAA99530.1. Frameshift.
AY260886 Genomic DNA. Translation: AAP21754.1.
AY692753 Genomic DNA. Translation: AAT92772.1.
BK006948 Genomic DNA. Translation: DAA11064.1.
PIRiS72394.
RefSeqiNP_014942.4. NM_001184346.3.

3D structure databases

ProteinModelPortaliO14467.
SMRiO14467.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34687. 73 interactors.
DIPiDIP-8708N.
IntActiO14467. 2 interactors.
MINTiMINT-8285541.

PTM databases

iPTMnetiO14467.

Proteomic databases

MaxQBiO14467.
PRIDEiO14467.
TopDownProteomicsiO14467.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR298C-A; YOR298C-A; YOR298C-A.
GeneIDi854474.
KEGGisce:YOR298C-A.

Organism-specific databases

EuPathDBiFungiDB:YOR298C-A.
SGDiS000007253. MBF1.

Phylogenomic databases

GeneTreeiENSGT00390000008519.
HOGENOMiHOG000195726.
InParanoidiO14467.
KOiK03627.
OMAiEPKMTQK.
OrthoDBiEOG092C5XAS.

Enzyme and pathway databases

BioCyciYEAST:G3O-33879-MONOMER.

Miscellaneous databases

PROiO14467.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
InterProiIPR001387. Cro/C1-type_HTH.
IPR010982. Lambda_DNA-bd_dom.
IPR013729. MBF1_N.
[Graphical view]
PfamiPF01381. HTH_3. 1 hit.
PF08523. MBF1. 1 hit.
[Graphical view]
SMARTiSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
PROSITEiPS50943. HTH_CROC1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMBF1_YEAST
AccessioniPrimary (citable) accession number: O14467
Secondary accession number(s): D6W2Z8, Q7LGJ8, Q86ZS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: May 1, 1999
Last modified: November 2, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 47277 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.