Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O14467

- MBF1_YEAST

UniProt

O14467 - MBF1_YEAST

Protein

Multiprotein-bridging factor 1

Gene

MBF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 2 (01 May 1999)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Transcriptional coactivator that stimulates GCN4-dependent transcriptional activity by bridging the DNA-binding region of GCN4 and TBP (SPT15), thereby recruiting TBP to GCN4-bound promoters.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi96 – 11520H-T-H motifPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. sequence-specific DNA binding Source: InterPro
    2. transcription coactivator activity Source: SGD

    GO - Biological processi

    1. positive regulation of transcription from RNA polymerase II promoter Source: SGD
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33879-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Multiprotein-bridging factor 1
    Alternative name(s):
    Suppressor of frameshift mutations protein 13
    Gene namesi
    Name:MBF1
    Synonyms:SUF13
    Ordered Locus Names:YOR298C-A
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR298c-a.
    SGDiS000007253. MBF1.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi112 – 1121D → A: Reduces interaction to TBP. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 151151Multiprotein-bridging factor 1PRO_0000149814Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei143 – 1431Phosphoserine5 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO14467.
    PaxDbiO14467.
    PeptideAtlasiO14467.

    Expressioni

    Gene expression databases

    GenevestigatoriO14467.

    Interactioni

    Subunit structurei

    Interacts with TBP and the transcription factor GCN4.1 Publication

    Protein-protein interaction databases

    BioGridi34687. 70 interactions.
    DIPiDIP-8708N.
    IntActiO14467. 2 interactions.
    MINTiMINT-8285541.

    Structurei

    3D structure databases

    ProteinModelPortaliO14467.
    SMRiO14467. Positions 55-151.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini85 – 13955HTH cro/C1-typePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni41 – 11979Essential for TBP-bindingAdd
    BLAST

    Sequence similaritiesi

    Belongs to the MBF1 family.Curated
    Contains 1 HTH cro/C1-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1813.
    GeneTreeiENSGT00390000008519.
    HOGENOMiHOG000195726.
    KOiK03627.
    OMAiTKVNEKP.
    OrthoDBiEOG7NGQQW.

    Family and domain databases

    Gene3Di1.10.260.40. 1 hit.
    InterProiIPR001387. Cro/C1-type_HTH.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013729. MBF1_N.
    [Graphical view]
    PfamiPF01381. HTH_3. 1 hit.
    PF08523. MBF1. 1 hit.
    [Graphical view]
    SMARTiSM00530. HTH_XRE. 1 hit.
    [Graphical view]
    SUPFAMiSSF47413. SSF47413. 1 hit.
    PROSITEiPS50943. HTH_CROC1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O14467-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDWDTNTII GSRARAGGSG PRANVARSQG QINAARRQGL VVSVDKKYGS    50
    TNTRGDNEGQ RLTKVDRETD IVKPKKLDPN VGRAISRART DKKMSQKDLA 100
    TKINEKPTVV NDYEAARAIP NQQVLSKLER ALGVKLRGNN IGSPLGAPKK 150
    K 151
    Length:151
    Mass (Da):16,404
    Last modified:May 1, 1999 - v2
    Checksum:iE687ED8768ACD1DF
    GO

    Sequence cautioni

    The sequence CAA99527.1 differs from that shown. Reason: Frameshift at position 20.
    The sequence CAA99530.1 differs from that shown. Reason: Frameshift at position 20.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB017593 Genomic DNA. Translation: BAA33217.1.
    Z75206 Genomic DNA. Translation: CAA99527.1. Frameshift.
    Z75207 Genomic DNA. Translation: CAA99530.1. Frameshift.
    AY260886 Genomic DNA. Translation: AAP21754.1.
    AY692753 Genomic DNA. Translation: AAT92772.1.
    BK006948 Genomic DNA. Translation: DAA11064.1.
    PIRiS72394.
    RefSeqiNP_014942.4. NM_001184346.3.

    Genome annotation databases

    EnsemblFungiiYOR298C-A; YOR298C-A; YOR298C-A.
    GeneIDi854474.
    KEGGisce:YOR298C-A.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB017593 Genomic DNA. Translation: BAA33217.1 .
    Z75206 Genomic DNA. Translation: CAA99527.1 . Frameshift.
    Z75207 Genomic DNA. Translation: CAA99530.1 . Frameshift.
    AY260886 Genomic DNA. Translation: AAP21754.1 .
    AY692753 Genomic DNA. Translation: AAT92772.1 .
    BK006948 Genomic DNA. Translation: DAA11064.1 .
    PIRi S72394.
    RefSeqi NP_014942.4. NM_001184346.3.

    3D structure databases

    ProteinModelPortali O14467.
    SMRi O14467. Positions 55-151.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34687. 70 interactions.
    DIPi DIP-8708N.
    IntActi O14467. 2 interactions.
    MINTi MINT-8285541.

    Proteomic databases

    MaxQBi O14467.
    PaxDbi O14467.
    PeptideAtlasi O14467.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR298C-A ; YOR298C-A ; YOR298C-A .
    GeneIDi 854474.
    KEGGi sce:YOR298C-A.

    Organism-specific databases

    CYGDi YOR298c-a.
    SGDi S000007253. MBF1.

    Phylogenomic databases

    eggNOGi COG1813.
    GeneTreei ENSGT00390000008519.
    HOGENOMi HOG000195726.
    KOi K03627.
    OMAi TKVNEKP.
    OrthoDBi EOG7NGQQW.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33879-MONOMER.

    Miscellaneous databases

    NextBioi 976777.
    PROi O14467.

    Gene expression databases

    Genevestigatori O14467.

    Family and domain databases

    Gene3Di 1.10.260.40. 1 hit.
    InterProi IPR001387. Cro/C1-type_HTH.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013729. MBF1_N.
    [Graphical view ]
    Pfami PF01381. HTH_3. 1 hit.
    PF08523. MBF1. 1 hit.
    [Graphical view ]
    SMARTi SM00530. HTH_XRE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47413. SSF47413. 1 hit.
    PROSITEi PS50943. HTH_CROC1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Yeast coactivator MBF1 mediates GCN4-dependent transcriptional activation."
      Takemaru K., Harashima S., Ueda H., Hirose S.
      Mol. Cell. Biol. 18:4971-4976(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH GCN4 AND TBP, MUTAGENESIS OF ASP-112, FUNCTION.
      Strain: KT130.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii."
      Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P.
      Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-58.
      Strain: ATCC 204511 / S288c / AB972.
    5. Bienvenut W.V., Peters C.
      Submitted (MAY-2005) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 76-83; 103-117 AND 138-149, IDENTIFICATION BY MASS SPECTROMETRY.
    6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 94-151.
      Strain: ATCC 204508 / S288c.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    11. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMBF1_YEAST
    AccessioniPrimary (citable) accession number: O14467
    Secondary accession number(s): D6W2Z8, Q7LGJ8, Q86ZS7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2005
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 47277 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3