Multiprotein-bridging factor 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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O14467 (MBF1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Multiprotein-bridging factor 1

Alternative name(s):
Suppressor of frameshift mutations protein 13

Gene names

Name:	MBF1
Synonyms:	SUF13
Ordered Locus Names:	YOR298C-A

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	151 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional coactivator that stimulates GCN4-dependent transcriptional activity by bridging the DNA-binding region of GCN4 and TBP (SPT15), thereby recruiting TBP to GCN4-bound promoters. Ref.1
Subunit structure	Interacts with TBP and the transcription factor GCN4. Ref.1
Subcellular location	Cytoplasm. Nucleus Ref.7.
Miscellaneous	Present with 47277 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the MBF1 family. Contains 1 HTH cro/C1-type DNA-binding domain.
Sequence caution	The sequence CAA99527.1 differs from that shown. Reason: Frameshift at position 20. The sequence CAA99530.1 differs from that shown. Reason: Frameshift at position 20.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.1. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrion Inferred from direct assay PubMed 14576278 PubMed 16823961. Source: SGD nucleus Inferred by curator Ref.1. Source: SGD
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription coactivator activity Inferred from direct assay Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 151

151

Multiprotein-bridging factor 1

PRO_0000149814

Regions

Domain

85 – 139

HTH cro/C1-type

DNA binding

96 – 115

H-T-H motif Potential

Region

41 – 119

Essential for TBP-binding

Amino acid modifications

Modified residue

108

Phosphothreonine Ref.13

Modified residue

143

Phosphoserine Ref.9 Ref.10 Ref.11 Ref.12 Ref.13

Experimental info

Mutagenesis

112

D → A: Reduces interaction to TBP. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

O14467 [UniParc].

Last modified May 1, 1999. Version 2.
Checksum: E687ED8768ACD1DF
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FASTA

151

16,404

        10         20         30         40         50         60 
MSDWDTNTII GSRARAGGSG PRANVARSQG QINAARRQGL VVSVDKKYGS TNTRGDNEGQ 

        70         80         90        100        110        120 
RLTKVDRETD IVKPKKLDPN VGRAISRART DKKMSQKDLA TKINEKPTVV NDYEAARAIP 

       130        140        150 
NQQVLSKLER ALGVKLRGNN IGSPLGAPKK K

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast coactivator MBF1 mediates GCN4-dependent transcriptional activation." Takemaru K., Harashima S., Ueda H., Hirose S. Mol. Cell. Biol. 18:4971-4976(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH GCN4 AND TBP, MUTAGENESIS OF ASP-112, FUNCTION. Strain: KT130.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii." Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P. Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-58. Strain: ATCC 204511 / S288c / AB972.
[5]	Bienvenut W.V., Peters C. Submitted (MAY-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 76-83; 103-117 AND 138-149, MASS SPECTROMETRY.
[6]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 94-151. Strain: ATCC 204508 / S288c.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, MASS SPECTROMETRY. Strain: YAL6B.
[10]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, MASS SPECTROMETRY. Strain: ADR376.
[11]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, MASS SPECTROMETRY.
[12]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, MASS SPECTROMETRY.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-108 AND SER-143, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB017593 Genomic DNA. Translation: BAA33217.1. Z75206 Genomic DNA. Translation: CAA99527.1. Frameshift. Z75207 Genomic DNA. Translation: CAA99530.1. Frameshift. AY260886 Genomic DNA. Translation: AAP21754.1. AY692753 Genomic DNA. Translation: AAT92772.1. BK006948 Genomic DNA. Translation: DAA11064.1.
PIR	S72394.
RefSeq	NP_014942.4. NM_001184346.3.
3D structure databases
ProteinModelPortal	O14467.
SMR	O14467. Positions 55-151.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-8708N.
IntAct	O14467. 1 interaction.
Proteomic databases
PaxDb	O14467.
PeptideAtlas	O14467.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR298C-A; YOR298C-A; YOR298C-A.
GeneID	854474.
KEGG	sce:YOR298C-A. sce:YOR301W.
Organism-specific databases
CYGD	YOR298c-a.
SGD	S000007253. MBF1.
Phylogenomic databases
eggNOG	COG1813.
GeneTree	ENSGT00390000008519.
HOGENOM	HOG000195726.
KO	K03627.
OMA	VIADYEC.
OrthoDB	EOG4MPN0H.
Gene expression databases
Genevestigator	O14467.
GermOnline	YOR298C-A. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.260.40. 1 hit.
InterPro	IPR001387. HTH. IPR010982. Lambda_DNA-bd_dom. IPR013729. MBF1_N. [Graphical view]
Pfam	PF01381. HTH_3. 1 hit. PF08523. MBF1. 1 hit. [Graphical view]
SMART	SM00530. HTH_XRE. 1 hit. [Graphical view]
SUPFAM	SSF47413. Lambda_like_DNA. 1 hit.
PROSITE	PS50943. HTH_CROC1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976777.

Entry information

Entry name

MBF1_YEAST

Accession

Primary (citable) accession number: O14467
Secondary accession number(s): D6W2Z8, Q7LGJ8, Q86ZS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents