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Protein

Elongation factor 2

Gene

eft201

more
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 33GTPBy similarity8
Nucleotide bindingi104 – 108GTPBy similarity5
Nucleotide bindingi158 – 161GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionElongation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-156902 Peptide chain elongation
R-SPO-5358493 Synthesis of diphthamide-EEF2
R-SPO-6798695 Neutrophil degranulation
R-SPO-8876725 Protein methylation

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 2
Short name:
EF-2
Gene namesi
Name:eft201
Synonyms:eft2, eft2-1
ORF Names:SPAC513.01c, SPAPYUK71.04c
AND
Name:eft202
ORF Names:SPCP31B10.07
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componentsi: Chromosome I, Chromosome III

Organism-specific databases

PomBaseiSPAC513.01c eft201
SPCP31B10.07 eft202

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000910231 – 842Elongation factor 2Add BLAST842

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei568Phosphoserine1 Publication1
Modified residuei574Phosphothreonine1 Publication1
Modified residuei699DiphthamideBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO14460
PaxDbiO14460
PRIDEiO14460

PTM databases

iPTMnetiO14460

Interactioni

Protein-protein interaction databases

BioGridi276105, 2 interactors
280559, 5 interactors
DIPiDIP-57891N
IntActiO14460, 2 interactors
MINTiO14460
STRINGi4896.SPCP31B10.07.1

Structurei

3D structure databases

ProteinModelPortaliO14460
SMRiO14460
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 253tr-type GPROSITE-ProRule annotationAdd BLAST237

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000231589
InParanoidiO14460
KOiK03234
OMAiEHLISGM
OrthoDBiEOG092C0D4V
PhylomeDBiO14460

Family and domain databases

Gene3Di3.30.230.10, 2 hits
InterProiView protein in InterPro
IPR035647 EFG_III/V
IPR000640 EFG_V-like
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR005517 Transl_elong_EFG/EF2_IV
PfamiView protein in Pfam
PF00679 EFG_C, 1 hit
PF03764 EFG_IV, 1 hit
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PRINTSiPR00315 ELONGATNFCT
SMARTiView protein in SMART
SM00838 EFG_C, 1 hit
SM00889 EFG_IV, 1 hit
SUPFAMiSSF50447 SSF50447, 1 hit
SSF52540 SSF52540, 1 hit
SSF54211 SSF54211, 1 hit
SSF54980 SSF54980, 2 hits
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

O14460-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAFTPEEVR NLMGKPSNVR NMSVIAHVDH GKSTLTDSLV QKAGIISAAK
60 70 80 90 100
AGDARFMDTR ADEQERGVTI KSTAISLFAE MTDDDMKDMK EPADGTDFLV
110 120 130 140 150
NLIDSPGHVD FSSEVTAALR VTDGALVVVD TIEGVCVQTE TVLRQALGER
160 170 180 190 200
IRPVVVVNKV DRALLELQIS QEELYQNFAR VVESVNVVIS TYYDKVLGDC
210 220 230 240 250
QVFPDKGTVA FASGLHGWAF TVRQFANRYA KKFGIDRNKM MQRLWGENYF
260 270 280 290 300
NPKTKKWSKS ATDANGNSNQ RAFNMFILDP IYRIFDAVMN SRKDEVFTLL
310 320 330 340 350
SKLEVTIKPD EKELEGKALL KVVMRKFLPA ADALMEMIVL HLPSPKTAQQ
360 370 380 390 400
YRAETLYEGP MDDECAVGIR NCDANAPLMI YVSKMVPTSD RGRFYAFGRV
410 420 430 440 450
FSGTVRSGLK VRIQGPNYVP GKKDDLFIKA IQRTVLMMGS RIEPIEDCPA
460 470 480 490 500
GNIIGLVGVD QFLVKSGTLT TSEVAHNMKV MKFSVSPVVQ VAVEVKNGND
510 520 530 540 550
LPKLVEGLKR LSKSDPCVLC TTSESGEHIV AGAGELHLEI CLKDLQEDHA
560 570 580 590 600
GIPLKISPPV VSYRESVSEP SSMTALSKSP NKHNRIFMTA EPMSEELSVA
610 620 630 640 650
IETGHVNPRD DFKVRARIMA DEFGWDVTDA RKIWCFGPDT TGANVVVDQT
660 670 680 690 700
KAVAYLNEIK DSVVAAFAWA SKEGPMFEEN LRSCRFNILD VVLHADAIHR
710 720 730 740 750
GGGQIIPTAR RVVYASTLLA SPIIQEPVFL VEIQVSENAM GGIYSVLNKK
760 770 780 790 800
RGHVFSEEQR VGTPLYNIKA YLPVNESFGF TGELRQATAG QAFPQLVFDH
810 820 830 840
WSPMSGDPLD PTSKPGQIVC EARKRKGLKE NVPDYTEYYD RL
Length:842
Mass (Da):93,231
Last modified:January 11, 2001 - v2
Checksum:iA544C5C454BC55C7
GO

Sequence cautioni

The sequence BAA13813 differs from that shown. Reason: Frameshift at position 701.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti323V → D in BAA13813 (PubMed:9501991).Curated1
Sequence conflicti464V → A in BAA13813 (PubMed:9501991).Curated1
Sequence conflicti821 – 823EAR → DVG in BAA23591 (PubMed:9099890).Curated3
Sequence conflicti821 – 823EAR → DVG in BAA23590 (PubMed:9099890).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83976 Genomic DNA Translation: BAA23591.1
D83975 Genomic DNA Translation: BAA23590.1
CU329672 Genomic DNA Translation: CAB58373.1
CU329670 Genomic DNA Translation: CAB52147.1
D89151 mRNA Translation: BAA13813.1 Frameshift.
PIRiT41697
RefSeqiNP_587863.1, NM_001022856.2
XP_001713073.1, XM_001713021.2

Genome annotation databases

EnsemblFungiiSPAC513.01c.1; SPAC513.01c.1:pep; SPAC513.01c
SPCP31B10.07.1; SPCP31B10.07.1:pep; SPCP31B10.07
GeneIDi2539544
KEGGispo:SPAC513.01c
spo:SPCP31B10.07

Similar proteinsi

Entry informationi

Entry nameiEF2_SCHPO
AccessioniPrimary (citable) accession number: O14460
Secondary accession number(s): P78802
, Q9USG7, Q9USZ9, Q9UT64
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 11, 2001
Last modified: March 28, 2018
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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