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Protein

Elongation factor 2

Gene

eft201

more
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 338GTPBy similarity
Nucleotide bindingi104 – 1085GTPBy similarity
Nucleotide bindingi158 – 1614GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: GO_Central
  • GTP binding Source: PomBase
  • translation elongation factor activity Source: PomBase

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-156902. Peptide chain elongation.
R-SPO-5358493. Synthesis of diphthamide-EEF2.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 2
Short name:
EF-2
Gene namesi
Name:eft201
Synonyms:eft2, eft2-1
ORF Names:SPAC513.01c, SPAPYUK71.04c
AND
Name:eft202
ORF Names:SPCP31B10.07
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componentsi: Chromosome I, Chromosome III

Organism-specific databases

PomBaseiSPAC513.01c. eft201.
SPCP31B10.07. eft202.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • intracellular Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 842842Elongation factor 2PRO_0000091023Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei568 – 5681Phosphoserine1 Publication
Modified residuei574 – 5741Phosphothreonine1 Publication
Modified residuei699 – 6991DiphthamideBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO14460.
PRIDEiO14460.

PTM databases

iPTMnetiO14460.

Interactioni

Protein-protein interaction databases

BioGridi276105. 2 interactions.
280559. 6 interactions.
DIPiDIP-57891N.
IntActiO14460. 1 interaction.
MINTiMINT-4672831.

Structurei

3D structure databases

ProteinModelPortaliO14460.
SMRiO14460. Positions 1-842.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 253237tr-type GPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000231589.
InParanoidiO14460.
KOiK03234.
OMAiTNNESSH.
OrthoDBiEOG092C0D4V.
PhylomeDBiO14460.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O14460-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAFTPEEVR NLMGKPSNVR NMSVIAHVDH GKSTLTDSLV QKAGIISAAK
60 70 80 90 100
AGDARFMDTR ADEQERGVTI KSTAISLFAE MTDDDMKDMK EPADGTDFLV
110 120 130 140 150
NLIDSPGHVD FSSEVTAALR VTDGALVVVD TIEGVCVQTE TVLRQALGER
160 170 180 190 200
IRPVVVVNKV DRALLELQIS QEELYQNFAR VVESVNVVIS TYYDKVLGDC
210 220 230 240 250
QVFPDKGTVA FASGLHGWAF TVRQFANRYA KKFGIDRNKM MQRLWGENYF
260 270 280 290 300
NPKTKKWSKS ATDANGNSNQ RAFNMFILDP IYRIFDAVMN SRKDEVFTLL
310 320 330 340 350
SKLEVTIKPD EKELEGKALL KVVMRKFLPA ADALMEMIVL HLPSPKTAQQ
360 370 380 390 400
YRAETLYEGP MDDECAVGIR NCDANAPLMI YVSKMVPTSD RGRFYAFGRV
410 420 430 440 450
FSGTVRSGLK VRIQGPNYVP GKKDDLFIKA IQRTVLMMGS RIEPIEDCPA
460 470 480 490 500
GNIIGLVGVD QFLVKSGTLT TSEVAHNMKV MKFSVSPVVQ VAVEVKNGND
510 520 530 540 550
LPKLVEGLKR LSKSDPCVLC TTSESGEHIV AGAGELHLEI CLKDLQEDHA
560 570 580 590 600
GIPLKISPPV VSYRESVSEP SSMTALSKSP NKHNRIFMTA EPMSEELSVA
610 620 630 640 650
IETGHVNPRD DFKVRARIMA DEFGWDVTDA RKIWCFGPDT TGANVVVDQT
660 670 680 690 700
KAVAYLNEIK DSVVAAFAWA SKEGPMFEEN LRSCRFNILD VVLHADAIHR
710 720 730 740 750
GGGQIIPTAR RVVYASTLLA SPIIQEPVFL VEIQVSENAM GGIYSVLNKK
760 770 780 790 800
RGHVFSEEQR VGTPLYNIKA YLPVNESFGF TGELRQATAG QAFPQLVFDH
810 820 830 840
WSPMSGDPLD PTSKPGQIVC EARKRKGLKE NVPDYTEYYD RL
Length:842
Mass (Da):93,231
Last modified:January 11, 2001 - v2
Checksum:iA544C5C454BC55C7
GO

Sequence cautioni

The sequence BAA13813 differs from that shown. Reason: Frameshift at position 701. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti323 – 3231V → D in BAA13813 (PubMed:9501991).Curated
Sequence conflicti464 – 4641V → A in BAA13813 (PubMed:9501991).Curated
Sequence conflicti821 – 8233EAR → DVG in BAA23591 (PubMed:9099890).Curated
Sequence conflicti821 – 8233EAR → DVG in BAA23590 (PubMed:9099890).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83976 Genomic DNA. Translation: BAA23591.1.
D83975 Genomic DNA. Translation: BAA23590.1.
CU329672 Genomic DNA. Translation: CAB58373.1.
CU329670 Genomic DNA. Translation: CAB52147.1.
D89151 mRNA. Translation: BAA13813.1. Frameshift.
PIRiT41697.
RefSeqiNP_587863.1. NM_001022856.2.
XP_001713073.1. XM_001713021.2.

Genome annotation databases

EnsemblFungiiSPAC513.01c.1; SPAC513.01c.1:pep; SPAC513.01c.
SPCP31B10.07.1; SPCP31B10.07.1:pep; SPCP31B10.07.
GeneIDi2539544.
3361483.
KEGGispo:SPAC513.01c.
spo:SPCP31B10.07.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83976 Genomic DNA. Translation: BAA23591.1.
D83975 Genomic DNA. Translation: BAA23590.1.
CU329672 Genomic DNA. Translation: CAB58373.1.
CU329670 Genomic DNA. Translation: CAB52147.1.
D89151 mRNA. Translation: BAA13813.1. Frameshift.
PIRiT41697.
RefSeqiNP_587863.1. NM_001022856.2.
XP_001713073.1. XM_001713021.2.

3D structure databases

ProteinModelPortaliO14460.
SMRiO14460. Positions 1-842.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276105. 2 interactions.
280559. 6 interactions.
DIPiDIP-57891N.
IntActiO14460. 1 interaction.
MINTiMINT-4672831.

PTM databases

iPTMnetiO14460.

Proteomic databases

MaxQBiO14460.
PRIDEiO14460.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC513.01c.1; SPAC513.01c.1:pep; SPAC513.01c.
SPCP31B10.07.1; SPCP31B10.07.1:pep; SPCP31B10.07.
GeneIDi2539544.
3361483.
KEGGispo:SPAC513.01c.
spo:SPCP31B10.07.

Organism-specific databases

PomBaseiSPAC513.01c. eft201.
SPCP31B10.07. eft202.

Phylogenomic databases

HOGENOMiHOG000231589.
InParanoidiO14460.
KOiK03234.
OMAiTNNESSH.
OrthoDBiEOG092C0D4V.
PhylomeDBiO14460.

Enzyme and pathway databases

ReactomeiR-SPO-156902. Peptide chain elongation.
R-SPO-5358493. Synthesis of diphthamide-EEF2.

Miscellaneous databases

PROiO14460.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEF2_SCHPO
AccessioniPrimary (citable) accession number: O14460
Secondary accession number(s): P78802
, Q9USG7, Q9USZ9, Q9UT64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 11, 2001
Last modified: September 7, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.