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Protein

DNA-directed RNA polymerase II subunit rpb7

Gene

rpb7

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranscription

Enzyme and pathway databases

ReactomeiR-SPO-113418. Formation of the Early Elongation Complex.
R-SPO-674695. RNA Polymerase II Pre-transcription Events.
R-SPO-6781823. Formation of TC-NER Pre-Incision Complex.
R-SPO-6782135. Dual incision in TC-NER.
R-SPO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SPO-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-SPO-72086. mRNA Capping.
R-SPO-72165. mRNA Splicing - Minor Pathway.
R-SPO-73776. RNA Polymerase II Promoter Escape.
R-SPO-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SPO-75953. RNA Polymerase II Transcription Initiation.
R-SPO-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SPO-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase II subunit rpb7
Short name:
RNA polymerase II subunit B7
Gene namesi
Name:rpb7
ORF Names:SPACUNK4.06c, SPAPYUK71.02
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPACUNK4.06c.
PomBaseiSPACUNK4.06c. rpb7.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi166E → A: Reduced interaction with seb1. 1 Publication1
Mutagenesisi167D → A: Reduced interaction with seb1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000739921 – 172DNA-directed RNA polymerase II subunit rpb7Add BLAST172

Proteomic databases

MaxQBiO14459.
PRIDEiO14459.

Interactioni

Subunit structurei

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB4 and RPB7 form a subcomplex that protrudes from the 10-subunit Pol II core complex (By similarity). Interacts with seb1.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
seb1Q9UTE33EBI-608029,EBI-608019

GO - Molecular functioni

Protein-protein interaction databases

BioGridi278573. 122 interactors.
IntActiO14459. 3 interactors.
MINTiMINT-1213963.
STRINGi4896.SPACUNK4.06c.1.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3H0GX-ray3.65G/S1-172[»]
5U0Selectron microscopy7.80g1-172[»]
ProteinModelPortaliO14459.
SMRiO14459.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO14459.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000158201.
InParanoidiO14459.
KOiK03015.
OMAiSYFGPRM.
OrthoDBiEOG092C4KGR.
PhylomeDBiO14459.

Family and domain databases

Gene3Di3.30.1490.120. 1 hit.
InterProiView protein in InterPro
IPR012340. NA-bd_OB-fold.
IPR036898. RNA_pol_Rpb7-like_N_sf.
IPR005576. RNA_pol_Rpb7_N.
IPR003029. S1_domain.
PfamiView protein in Pfam
PF00575. S1. 1 hit.
PF03876. SHS2_Rpb7-N. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF88798. SSF88798. 1 hit.

Sequencei

Sequence statusi: Complete.

O14459-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFFLKELSL TISLHPSYFG PRMQDYLKAK LLADVEGTCS GQYGYIICVL
60 70 80 90 100
DSNTIDIDKG RVVPGQGFAE FEVKYRAVLW RPFRGEVVDA IVTTVNKMGF
110 120 130 140 150
FANIGPLNVF VSSHLVPPDM KFDPTANPPN YSGEDQVIEK GSNVRLKIVG
160 170
TRTDATEIFA IATMKEDYLG VL
Length:172
Mass (Da):19,103
Last modified:January 1, 1998 - v1
Checksum:i0AECC46B9499FD33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89594 Genomic DNA. Translation: BAA22804.1.
D86554 mRNA. Translation: BAA22802.1.
AF027821 mRNA. Translation: AAB92516.1.
AF055916 Genomic DNA. Translation: AAC99317.1.
CU329670 Genomic DNA. Translation: CAA20136.1.
PIRiT39254.
RefSeqiXP_001713072.1. XM_001713020.2.

Genome annotation databases

EnsemblFungiiSPACUNK4.06c.1; SPACUNK4.06c.1:pep; SPACUNK4.06c.
KEGGispo:SPACUNK4.06c.

Similar proteinsi

Entry informationi

Entry nameiRPB7_SCHPO
AccessioniPrimary (citable) accession number: O14459
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 22, 2017
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families