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Protein

DNA-directed RNA polymerase II subunit rpb7

Gene

rpb7

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • regulation of cell separation after cytokinesis Source: PomBase
  • RNA processing Source: PomBase
  • telomeric repeat-containing RNA transcription from RNA pol II promoter Source: PomBase
  • transcription initiation from RNA polymerase II promoter Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Transcription

Enzyme and pathway databases

ReactomeiR-SPO-113418. Formation of the Early Elongation Complex.
R-SPO-674695. RNA Polymerase II Pre-transcription Events.
R-SPO-6781823. Formation of TC-NER Pre-Incision Complex.
R-SPO-6782135. Dual incision in TC-NER.
R-SPO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SPO-72086. mRNA Capping.
R-SPO-72165. mRNA Splicing - Minor Pathway.
R-SPO-73776. RNA Polymerase II Promoter Escape.
R-SPO-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SPO-75953. RNA Polymerase II Transcription Initiation.
R-SPO-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SPO-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase II subunit rpb7
Short name:
RNA polymerase II subunit B7
Gene namesi
Name:rpb7
ORF Names:SPACUNK4.06c, SPAPYUK71.02
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPACUNK4.06c.
PomBaseiSPACUNK4.06c. rpb7.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • DNA-directed RNA polymerase II, holoenzyme Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi166 – 1661E → A: Reduced interaction with seb1. 1 Publication
Mutagenesisi167 – 1671D → A: Reduced interaction with seb1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 172172DNA-directed RNA polymerase II subunit rpb7PRO_0000073992Add
BLAST

Proteomic databases

MaxQBiO14459.

Interactioni

Subunit structurei

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB4 and RPB7 form a subcomplex that protrudes from the 10-subunit Pol II core complex (By similarity). Interacts with seb1.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
seb1Q9UTE33EBI-608029,EBI-608019

Protein-protein interaction databases

BioGridi278573. 121 interactions.
IntActiO14459. 3 interactions.
MINTiMINT-1213963.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H0GX-ray3.65G/S1-172[»]
ProteinModelPortaliO14459.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO14459.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000158201.
InParanoidiO14459.
KOiK03015.
OMAiGQAEFVT.
OrthoDBiEOG7X0VTZ.
PhylomeDBiO14459.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.1490.120. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR005576. RNA_pol_Rpb7_N.
IPR003029. S1_domain.
[Graphical view]
PfamiPF00575. S1. 1 hit.
PF03876. SHS2_Rpb7-N. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF88798. SSF88798. 1 hit.

Sequencei

Sequence statusi: Complete.

O14459-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFFLKELSL TISLHPSYFG PRMQDYLKAK LLADVEGTCS GQYGYIICVL
60 70 80 90 100
DSNTIDIDKG RVVPGQGFAE FEVKYRAVLW RPFRGEVVDA IVTTVNKMGF
110 120 130 140 150
FANIGPLNVF VSSHLVPPDM KFDPTANPPN YSGEDQVIEK GSNVRLKIVG
160 170
TRTDATEIFA IATMKEDYLG VL
Length:172
Mass (Da):19,103
Last modified:January 1, 1998 - v1
Checksum:i0AECC46B9499FD33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89594 Genomic DNA. Translation: BAA22804.1.
D86554 mRNA. Translation: BAA22802.1.
AF027821 mRNA. Translation: AAB92516.1.
AF055916 Genomic DNA. Translation: AAC99317.1.
CU329670 Genomic DNA. Translation: CAA20136.1.
PIRiT39254.
RefSeqiXP_001713072.1. XM_001713020.2.

Genome annotation databases

EnsemblFungiiSPACUNK4.06c.1; SPACUNK4.06c.1:pep; SPACUNK4.06c.
GeneIDi2542097.
KEGGispo:SPACUNK4.06c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89594 Genomic DNA. Translation: BAA22804.1.
D86554 mRNA. Translation: BAA22802.1.
AF027821 mRNA. Translation: AAB92516.1.
AF055916 Genomic DNA. Translation: AAC99317.1.
CU329670 Genomic DNA. Translation: CAA20136.1.
PIRiT39254.
RefSeqiXP_001713072.1. XM_001713020.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H0GX-ray3.65G/S1-172[»]
ProteinModelPortaliO14459.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278573. 121 interactions.
IntActiO14459. 3 interactions.
MINTiMINT-1213963.

Proteomic databases

MaxQBiO14459.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPACUNK4.06c.1; SPACUNK4.06c.1:pep; SPACUNK4.06c.
GeneIDi2542097.
KEGGispo:SPACUNK4.06c.

Organism-specific databases

EuPathDBiFungiDB:SPACUNK4.06c.
PomBaseiSPACUNK4.06c. rpb7.

Phylogenomic databases

HOGENOMiHOG000158201.
InParanoidiO14459.
KOiK03015.
OMAiGQAEFVT.
OrthoDBiEOG7X0VTZ.
PhylomeDBiO14459.

Enzyme and pathway databases

ReactomeiR-SPO-113418. Formation of the Early Elongation Complex.
R-SPO-674695. RNA Polymerase II Pre-transcription Events.
R-SPO-6781823. Formation of TC-NER Pre-Incision Complex.
R-SPO-6782135. Dual incision in TC-NER.
R-SPO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SPO-72086. mRNA Capping.
R-SPO-72165. mRNA Splicing - Minor Pathway.
R-SPO-73776. RNA Polymerase II Promoter Escape.
R-SPO-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SPO-75953. RNA Polymerase II Transcription Initiation.
R-SPO-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SPO-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

EvolutionaryTraceiO14459.
PROiO14459.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.1490.120. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR005576. RNA_pol_Rpb7_N.
IPR003029. S1_domain.
[Graphical view]
PfamiPF00575. S1. 1 hit.
PF03876. SHS2_Rpb7-N. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF88798. SSF88798. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gene organization and protein sequence of the small subunits of Schizosaccharomyces pombe RNA polymerase II."
    Sakurai H., Ishihama A.
    Gene 196:165-174(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 972 / ATCC 24843 and JY741.
  2. "Molecular cloning of rpb5+, rpb7+ and rpb11+ genes of the fission yeast Schizosaccharomyces pombe: completing primary structure of all indispensable subunits of its RNA polymerase II."
    Shpakovski G.V., Lebedenko E.N.
    Bioorg. Khim. 23:988-991(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 972 / ATCC 24843.
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. "Rpb7 subunit of RNA polymerase II interacts with an RNA-binding protein involved in processing of transcripts."
    Mitsuzawa H., Kanda E., Ishihama A.
    Nucleic Acids Res. 31:4696-4701(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SEB1, MUTAGENESIS OF GLU-166 AND ASP-167.

Entry informationi

Entry nameiRPB7_SCHPO
AccessioniPrimary (citable) accession number: O14459
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: June 8, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.