Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA-directed RNA polymerase II subunit rpb7

Gene

rpb7

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranscription

Enzyme and pathway databases

ReactomeiR-SPO-113418 Formation of the Early Elongation Complex
R-SPO-5578749 Transcriptional regulation by small RNAs
R-SPO-674695 RNA Polymerase II Pre-transcription Events
R-SPO-6781823 Formation of TC-NER Pre-Incision Complex
R-SPO-6782135 Dual incision in TC-NER
R-SPO-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SPO-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-SPO-72086 mRNA Capping
R-SPO-72165 mRNA Splicing - Minor Pathway
R-SPO-73776 RNA Polymerase II Promoter Escape
R-SPO-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SPO-75953 RNA Polymerase II Transcription Initiation
R-SPO-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-SPO-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-SPO-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase II subunit rpb7
Short name:
RNA polymerase II subunit B7
Gene namesi
Name:rpb7
ORF Names:SPACUNK4.06c, SPAPYUK71.02
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPACUNK4.06c
PomBaseiSPACUNK4.06c rpb7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi166E → A: Reduced interaction with seb1. 1 Publication1
Mutagenesisi167D → A: Reduced interaction with seb1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000739921 – 172DNA-directed RNA polymerase II subunit rpb7Add BLAST172

Proteomic databases

MaxQBiO14459
PaxDbiO14459
PRIDEiO14459

Interactioni

Subunit structurei

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB4 and RPB7 form a subcomplex that protrudes from the 10-subunit Pol II core complex (By similarity). Interacts with seb1.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
seb1Q9UTE33EBI-608029,EBI-608019

GO - Molecular functioni

Protein-protein interaction databases

BioGridi278573, 122 interactors
IntActiO14459, 3 interactors
MINTiO14459
STRINGi4896.SPACUNK4.06c.1

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3H0GX-ray3.65G/S1-172[»]
5U0Selectron microscopy7.80g1-172[»]
ProteinModelPortaliO14459
SMRiO14459
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO14459

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000158201
InParanoidiO14459
KOiK03015
OMAiMRQPGLG
OrthoDBiEOG092C4KGR
PhylomeDBiO14459

Family and domain databases

Gene3Di3.30.1490.120, 1 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR036898 RNA_pol_Rpb7-like_N_sf
IPR005576 RNA_pol_Rpb7_N
IPR003029 S1_domain
PfamiView protein in Pfam
PF00575 S1, 1 hit
PF03876 SHS2_Rpb7-N, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF88798 SSF88798, 1 hit

Sequencei

Sequence statusi: Complete.

O14459-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFFLKELSL TISLHPSYFG PRMQDYLKAK LLADVEGTCS GQYGYIICVL
60 70 80 90 100
DSNTIDIDKG RVVPGQGFAE FEVKYRAVLW RPFRGEVVDA IVTTVNKMGF
110 120 130 140 150
FANIGPLNVF VSSHLVPPDM KFDPTANPPN YSGEDQVIEK GSNVRLKIVG
160 170
TRTDATEIFA IATMKEDYLG VL
Length:172
Mass (Da):19,103
Last modified:January 1, 1998 - v1
Checksum:i0AECC46B9499FD33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89594 Genomic DNA Translation: BAA22804.1
D86554 mRNA Translation: BAA22802.1
AF027821 mRNA Translation: AAB92516.1
AF055916 Genomic DNA Translation: AAC99317.1
CU329670 Genomic DNA Translation: CAA20136.1
PIRiT39254
RefSeqiXP_001713072.1, XM_001713020.2

Genome annotation databases

EnsemblFungiiSPACUNK4.06c.1; SPACUNK4.06c.1:pep; SPACUNK4.06c
KEGGispo:SPACUNK4.06c

Similar proteinsi

Entry informationi

Entry nameiRPB7_SCHPO
AccessioniPrimary (citable) accession number: O14459
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health