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Protein

cAMP-dependent protein kinase regulatory subunit

Gene

SUM1

Organism
Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei208 – 2081cAMP 1By similarity
Binding sitei217 – 2171cAMP 1By similarity
Binding sitei329 – 3291cAMP 2By similarity
Binding sitei338 – 3381cAMP 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi130 – 261132cAMP 1Add
BLAST
Nucleotide bindingi262 – 383122cAMP 2Add
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

cAMP, cAMP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase regulatory subunit
Short name:
PKA regulatory subunit
Gene namesi
Name:SUM1
Synonyms:RPKA
ORF Names:MGG_07335
OrganismiMagnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Taxonomic identifieri242507 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeMagnaporthalesMagnaporthaceaeMagnaporthe
Proteomesi
  • UP000009058 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiFungiDB:MGG_07335.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi211 – 2111L → R in SUM1-99; restores cAMP-stimulated appressorium formation and growth morphogenesis to adenylate cyclase-deficient mutants. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 390390cAMP-dependent protein kinase regulatory subunitPRO_0000205411Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei90 – 901PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Tetramer, composed of 2 regulatory (R) and 2 catalytic (C) subunits. In the presence of cAMP it dissociates into 2 active monomeric C subunits and an R dimer (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliO14448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 129129Dimerization and phosphorylationSequence analysisAdd
BLAST

Sequence similaritiesi

Contains 2 cyclic nucleotide-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

InParanoidiO14448.
KOiK04739.
OrthoDBiEOG7034TC.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR012198. cAMP_dep_PK_reg_su.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
[Graphical view]
PIRSFiPIRSF000548. PK_regulatory. 1 hit.
SMARTiSM00100. cNMP. 2 hits.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 2 hits.
PROSITEiPS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O14448-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASGFTSPF GANSNPFGSP EERRGVAGSI QPVLEEEEAD GIGSEPISFK
60 70 80 90 100
SPGFRAPFGG GDANSEGPPS SRPQNPDGYP AQYNFARRTS VSAESLKPIA
110 120 130 140 150
DSYDNWTPPF HPKSAEQLDR LKKAIQGNFL FSHLDDEQSA QILGALVEKP
160 170 180 190 200
IPAKDIKVIV QGDAGDYFYV VEKGKFSVHV NSSGVMQAGT QGLGDHVGTI
210 220 230 240 250
EAGGSFGELA LMYNAPRAAT VMSAEPNCVL WALDRVTFRR ILMESTFSRR
260 270 280 290 300
RMYENFLEEV PILSTLTAYE RSKIADALET QKYPPGTVVI KEGDPGEDFY
310 320 330 340 350
LLECGEAEAF KAGIDQPVKL YKKGDFFGEL ALLNDAPRAA SVVSKTEVKV
360 370 380 390
AALGKSAFQR LLGPVEPIMR RTRYDAIKTG VEEMDPLQAA
Length:390
Mass (Da):42,271
Last modified:January 1, 1998 - v1
Checksum:i1BA8BD8A21439A8F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024633 Genomic DNA. Translation: AAC34140.1.
AF015753 Genomic DNA. Translation: AAB70215.1.
CM001232 Genomic DNA. Translation: EHA55765.1.
RefSeqiXP_003715572.1. XM_003715524.1.

Genome annotation databases

EnsemblFungiiMGG_07335T0; MGG_07335T0; MGG_07335.
GeneIDi2683231.
KEGGimgr:MGG_07335.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024633 Genomic DNA. Translation: AAC34140.1.
AF015753 Genomic DNA. Translation: AAB70215.1.
CM001232 Genomic DNA. Translation: EHA55765.1.
RefSeqiXP_003715572.1. XM_003715524.1.

3D structure databases

ProteinModelPortaliO14448.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiMGG_07335T0; MGG_07335T0; MGG_07335.
GeneIDi2683231.
KEGGimgr:MGG_07335.

Organism-specific databases

EuPathDBiFungiDB:MGG_07335.

Phylogenomic databases

InParanoidiO14448.
KOiK04739.
OrthoDBiEOG7034TC.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR012198. cAMP_dep_PK_reg_su.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
[Graphical view]
PIRSFiPIRSF000548. PK_regulatory. 1 hit.
SMARTiSM00100. cNMP. 2 hits.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 2 hits.
PROSITEiPS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Divergent cAMP signaling pathways regulate growth and pathogenesis in the rice blast fungus Magnaporthe grisea."
    Adachi K., Hamer J.E.
    Plant Cell 10:1361-1374(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF LEU-211.
    Strain: Guyane 11.
  2. "The cAMP-dependent protein kinase regulatory subunit gene from Magnaporthe grisea."
    Gilbert R.D., Dean R.A.
    Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 70-15 / ATCC MYA-4617 / FGSC 8958.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 70-15 / ATCC MYA-4617 / FGSC 8958.

Entry informationi

Entry nameiKAPR_MAGO7
AccessioniPrimary (citable) accession number: O14448
Secondary accession number(s): A4RIF5, G4MVD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 11, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.