Reviewed, UniProtKB/Swiss-Prot O14442 (CP51_UNCNE)

Names and origin

Protein names

Recommended name:
    Eburicol 14-alpha-demethylase
    EC=1.14.13.70
Alternative name(s):
    Cytochrome P450 51
    CYPLI
    Cytochrome P450-LIA1
    Sterol 14-alpha demethylase
    Cytochrome P450-14DM

Gene names

Name:

CYP51

Organism

Uncinula necator (Grape powdery mildew)

Taxonomic identifier

52586 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Leotiomycetes › Erysiphales › Erysiphaceae › Erysiphe

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol By similarity.
Catalytic activity	Obtusifoliol + 3 O2 + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3 NADP+ + 4 H2O.
Cofactor	Heme group By similarity.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.
Subcellular location	Membrane Potential. Membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sterol 14-demethylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 524	524	Eburicol 14-alpha-demethylase		PRO_0000052010
Sites
	Metal binding	469	1	Iron (heme axial ligand) By similarity
Natural variations
	Natural variant	136	1	Y → F in strain: PAZ11; resistant to triadimenol; a sterol demethylation-inhibiting fungicide. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O14442-1 [UniParc]. Last modified July 11, 2001. Version 2. Checksum: 24BE154B176CE09A	FASTA	524	59,840
	10 20 30 40 50 60 MYIADILSDL LTQQTTRYGW IFMVTSIAFS IILLAVGLNV LSQLLFRRPY EPPVVFHWFP 70 80 90 100 110 120 IIGSTISYGI DPYKFYFDCR AKYGDIFTFI LLGKKVTVYL GLQGNNFILN GKLKDVNAEE 130 140 150 160 170 180 IYTNLTTPVF GRDVVYDCPN SKLMEQKKFM KTALTIEAFH SYVTIIQNEV EAYINNCVSF 190 200 210 220 230 240 QGESGTVNIS KVMAEITIYT ASHALQGEEV RENFDSSFAA LYHDLDMGFT PINFTFYWAP 250 260 270 280 290 300 LPWNRARDHA QRTVARTYMN IIQARREEKR SGENKHDIMW ELMRSTYKDG TPVPDREIAH 310 320 330 340 350 360 MMIALLMAGQ HSSSSTSSWI MLWLAARPDI MEELYEEQLR IFGSEKPFPP LQYEDLSKLQ 370 380 390 400 410 420 LHQNVLKEVL RLHAPIHSIM RKVKNPMIVP GTKYVIPTSH VLISSPGCTS QDATFFPDPL 430 440 450 460 470 480 KWDPHRWDIG SGKVLGNDAV DEKYDYGYGL TSTGASSPYL PFGAGRHRCI GEQFATLQLV 490 500 510 520 TIMATMVRFF RFRNIDGKQG VVKTDYSSLF SMPLAPALIG WEKR

References

[1]	"Cloning and sequence analysis of the eburicol 14alpha-demethylase gene of the obligate biotrophic grape powdery mildew fungus." Delye C., Laigret F., Corio-Costet M.-F. Gene 195:29-33(1997) [PubMed: 9300816] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANT PHE-136. Strain: FPE11.
[2]	Delye C., Laigret F., Corio-Costet M.-F. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 515-517.
[3]	"A mutation in the 14 alpha-demethylase gene of Uncinula necator that correlates with resistance to a sterol biosynthesis inhibitor." Delye C., Laigret F., Corio-Costet M.-F. Appl. Environ. Microbiol. 63:2966-2970(1997) [PubMed: 9251183] [Abstract] Cited for: MOLECULAR BASIS OF FUNGICIDE RESISTANCE. Strain: PAZ11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U72657 Genomic DNA. Translation: AAC49811.2. U72658 mRNA. Translation: AAC49812.2. U83840 Genomic DNA. Translation: AAC49801.2.
3D structure databases
SMR	O14442. Positions 52-524.
ModBase	Search...
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP51_UNCNE

Accession

Primary (citable) accession number: O14442
Secondary accession number(s): O14422

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 11, 2001
Last modified:	January 19, 2010
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)