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O14429 (LEU3_CANGA) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
3-isopropylmalate dehydrogenase
Short name=3-IPM-DH
Short name=IMDH
EC=1.1.1.85
Alternative name(s):
Beta-IPM dehydrogenase
Gene names
Name:LEU2
Ordered Locus Names:CAGL0H03795g
OrganismCandida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata) [Complete proteome]
Taxonomic identifier284593 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Nakaseomyces › mitosporic Nakaseomyces

Protein attributes

Sequence length365 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.

Catalytic activity

(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH.

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3653653-isopropylmalate dehydrogenase
PRO_0000083603

Regions

Nucleotide binding80 – 9112NAD By similarity
Nucleotide binding290 – 30112NAD By similarity

Sites

Metal binding2261Magnesium or manganese By similarity
Metal binding2511Magnesium or manganese By similarity
Metal binding2551Magnesium or manganese By similarity
Binding site981Substrate By similarity
Binding site1081Substrate By similarity
Binding site1371Substrate By similarity
Binding site2261Substrate By similarity
Site1441Important for catalysis By similarity
Site1931Important for catalysis By similarity

Experimental info

Sequence conflict101L → P in AAB62089. Ref.1
Sequence conflict241I → N in AAB62089. Ref.1
Sequence conflict511A → P in AAB62089. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O14429 [UniParc].

Last modified August 16, 2004. Version 2.
Checksum: 05D59E0986360B82

FASTA36539,305
        10         20         30         40         50         60 
MAVTKTIVVL PGDHVGQEIT EEAIKVLNAI QECRPDKVNF KFDHHLIGGA AIDATGVPLP 

        70         80         90        100        110        120 
DEALEASKKA DAVLLGAVGG PKWGTGAVRP EQGLLKIRKE LQLYANLRPC NFASDSLLDL 

       130        140        150        160        170        180 
SPLKPEIARG TDFVVVRELV GGIYFGERKE DEGDGVAWDS EKYSVPEVQR ITRMAAFMAL 

       190        200        210        220        230        240 
QHNPPLPIWS LDKANVLASS RLWRKTVEET IKNEFPQLTV QHQLIDSAAM ILVKNPTHLN 

       250        260        270        280        290        300 
GIIITNNMFG DIISDEASVI PGSLGLLPSA SLASLPDKNT AFGLYEPCHG SAPDLPKGKV 

       310        320        330        340        350        360 
NPVATILSAA MMLKLSLDLF EEGEIIEQAV KKVLDSGIRT ADLKGTNSTT EVGDAVAKAV 


RELLA

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U90626 Genomic DNA. Translation: AAB62089.1.
CR380954 Genomic DNA. Translation: CAG59892.1.
RefSeqXP_446959.1. XM_446959.1.

3D structure databases

ProteinModelPortalO14429.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2888798.
GenomeReviewsGene locus CAGL0H03795g in contig CR380954_GR.
KEGGcgr:CAGL0H03795g.

Phylogenomic databases

eggNOGfuNOG04288.
HOGENOMHBG518924.
OMAFESARIR.
OrthoDBEOG4897W6.

Family and domain databases

InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
IPR004429. Isopropylmalate_DH.
[Graphical view]
Gene3DG3DSA:3.40.718.10. IDH_IMDH. 1 hit.
KOK00052.
PANTHERPTHR11835. IDH_IMDH_dimeric. 1 hit.
PTHR11835:SF13. IPMDH. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsTIGR00169. LeuB. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEU3_CANGA
AccessionPrimary (citable) accession number: O14429
Secondary accession number(s): Q6FS35
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: August 16, 2004
Last modified: December 14, 2011
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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