ID   CBH1_SCHPO              Reviewed;         514 AA.
AC   O14423; O14294;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 2.
DT   22-SEP-2009, entry version 71.
DE   RecName: Full=CENP-B homolog protein 1;
DE            Short=CBHP-1;
GN   Name=cbh1; Synonyms=cbh; ORFNames=SPAC9E9.10c;
OS   Schizosaccharomyces pombe (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales;
OC   Schizosaccharomycetaceae; Schizosaccharomyces.
OX   NCBI_TaxID=4896;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, AND FUNCTION.
RC   STRAIN=ATCC 38366 / 972;
RX   MEDLINE=97385119; PubMed=9237993; DOI=10.1073/pnas.94.16.8427;
RA   Lee J.-K., Huberman J.A., Hurwitz J.;
RT   "Purification and characterization of a CENP-B homologue protein that
RT   binds to the centromeric K-type repeat DNA of Schizosaccharomyces
RT   pombe.";
RL   Proc. Natl. Acad. Sci. U.S.A. 94:8427-8432(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 38366 / 972;
RX   MEDLINE=21848401; PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M.,
RA   Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A.,
RA   Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G.,
RA   Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K.,
RA   James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J.,
RA   Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C.,
RA   Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E.,
RA   Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S.,
RA   Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K.,
RA   Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S.,
RA   Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B.,
RA   Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S.,
RA   Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D.,
RA   Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R.,
RA   Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B.,
RA   Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S.,
RA   Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M.,
RA   Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G.,
RA   Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J.,
RA   Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L.,
RA   Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J.,
RA   Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- FUNCTION: Binds to centromeric K-type repeat DNA and ARS3002 DNA.
CC       The CBH-binding consensus sequence is Py-Pu-A-T-A-T-Py-Pu-T-A.
CC   -!- SUBCELLULAR LOCATION: Nucleus. Centromere.
CC   -!- SIMILARITY: Contains 1 HTH CENPB-type DNA-binding domain.
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DR   EMBL; U86754; AAB80698.1; -; mRNA.
DR   EMBL; CU329670; CAB16408.1; -; Genomic_DNA.
DR   PIR; T39217; T39217.
DR   RefSeq; NP_594583.1; -.
DR   HSSP; P49777; 1IUF.
DR   STRING; O14423; -.
DR   GeneID; 2543290; -.
DR   GenomeReviews; CU329670_GR; cbh1.
DR   KEGG; spo:SPAC9E9.10c; -.
DR   NMPDR; fig|4896.1.peg.4553; -.
DR   GeneDB_Spombe; SPAC9E9.10c; -.
DR   BioCyc; SPOM-XXX-01:SPOM-XXX-01-002679-MON; -.
DR   ArrayExpress; O14423; -.
DR   GO; GO:0000780; C:condensed nuclear chromosome, centromeric r...; IDA:GeneDB_SPombe.
DR   GO; GO:0000790; C:nuclear chromatin; NAS:GeneDB_SPombe.
DR   GO; GO:0005819; C:spindle; IDA:GeneDB_SPombe.
DR   GO; GO:0019237; F:centromeric DNA binding; IDA:GeneDB_SPombe.
DR   GO; GO:0046983; F:protein dimerization activity; NAS:GeneDB_SPombe.
DR   GO; GO:0030702; P:chromatin silencing at centromere; IMP:GeneDB_SPombe.
DR   GO; GO:0007059; P:chromosome segregation; IGI:GeneDB_SPombe.
DR   GO; GO:0016570; P:histone modification; IMP:GeneDB_SPombe.
DR   InterPro; IPR015175; Centromere_CenpB_DNA-db_2.
DR   InterPro; IPR006600; Centromere_CenpB_HTH.
DR   InterPro; IPR004875; DDE_SF_endonuclease_CENPB-like.
DR   InterPro; IPR012287; Homeodomain-rel.
DR   Gene3D; G3DSA:1.10.10.60; Homeodomain-rel; 2.
DR   Pfam; PF09091; CenpB-DNA-bind; 1.
DR   Pfam; PF03184; DDE; 1.
DR   SMART; SM00674; CENPB; 1.
DR   PROSITE; PS51253; HTH_CENPB; 1.
PE   1: Evidence at protein level;
KW   Centromere; Complete proteome; Direct protein sequencing; DNA-binding;
KW   Nucleus.
FT   CHAIN         1    514       CENP-B homolog protein 1.
FT                                /FTId=PRO_0000126134.
FT   DOMAIN       69    144       HTH CENPB-type.
FT   COMPBIAS    421    493       Asp/Glu-rich (acidic).
FT   CONFLICT    168    168       I -> M (in Ref. 1; AAB80698).
SQ   SEQUENCE   514 AA;  59865 MW;  A48102EBAD0BB1E3 CRC64;
     MPPIRQAVSL AEKKAIRDYY NQSAHRIPQK EVTEWFRKTY NKDLSQSTIS EILSPKYSYL
     DDGSVRLGDI KKIRAPKFPL LDNAVYEWLQ QREVEGLPIS GDMIKQAATR FWSKIPAYAS
     LPLPDFSNGW LDKFRRRHYI QQSAVNQALE SIKFEVFREY PVHIQEFIRP YAQKDIFCVG
     GTSLFWKLTP NKQNLDYQEI QGMKRGKAKV SLIMCCNADG SERLPLWIVG YAQNPRSFEQ
     CGIFPKSMNF EWKWNGRASI SPIIMEEWLR WFDTRMQGRK VLLMLESNDT YQFGVESVRR
     FKGGFQNTSV FRIPEKTLDI KSPFEQGIVD TFKANYRRYW LQYSLNQINI LRDPLKAVNV
     LKAIRWMLWS WNFGVSEKTI QASFLRSGLL GPHSEAEGQG MESYQKAIME IGNLISSKGS
     EAVKQINEYI RPSEEDETKL HQDAVDTVAA EFLEERRFES DEEEDVEPQI SNVEAAMAFE
     VLIKYFEQHE NGDPSFVLDL YRRQRVIAPN NLIA
//