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Protein

CENP-B homolog protein 1

Gene

cbh1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds to centromeric K-type repeat DNA and ARS3002 DNA. The CBH-binding consensus sequence is Py-Pu-A-T-A-T-Py-Pu-T-A.1 Publication

GO - Molecular functioni

  • centromeric DNA binding Source: PomBase

GO - Biological processi

  • chromatin silencing at centromere Source: PomBase
  • histone modification Source: PomBase
  • mitotic sister chromatid segregation Source: PomBase
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CENP-B homolog protein 1
Short name:
CBHP-1
Gene namesi
Name:cbh1
Synonyms:cbh
ORF Names:SPAC9E9.10c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC9E9.10c.
PomBaseiSPAC9E9.10c. cbh1.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome, centromeric region Source: PomBase
  • mitotic spindle Source: PomBase
  • mitotic spindle pole body Source: PomBase
  • nuclear chromatin Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 514514CENP-B homolog protein 1PRO_0000126134Add
BLAST

Proteomic databases

MaxQBiO14423.

Interactioni

Protein-protein interaction databases

BioGridi279715. 78 interactions.
MINTiMINT-4672787.

Structurei

3D structure databases

ProteinModelPortaliO14423.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini69 – 14476HTH CENPB-typePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi421 – 49373Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Contains 1 HTH CENPB-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000033772.
InParanoidiO14423.
OMAiATINYEA.
OrthoDBiEOG7BZW1V.
PhylomeDBiO14423.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
InterProiIPR004875. DDE_SF_endonuclease_dom.
IPR009057. Homeodomain-like.
IPR006600. HTH_CenpB_DNA-bd_dom.
[Graphical view]
PfamiPF03184. DDE_1. 1 hit.
PF03221. HTH_Tnp_Tc5. 1 hit.
[Graphical view]
SMARTiSM00674. CENPB. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiPS51253. HTH_CENPB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O14423-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPIRQAVSL AEKKAIRDYY NQSAHRIPQK EVTEWFRKTY NKDLSQSTIS
60 70 80 90 100
EILSPKYSYL DDGSVRLGDI KKIRAPKFPL LDNAVYEWLQ QREVEGLPIS
110 120 130 140 150
GDMIKQAATR FWSKIPAYAS LPLPDFSNGW LDKFRRRHYI QQSAVNQALE
160 170 180 190 200
SIKFEVFREY PVHIQEFIRP YAQKDIFCVG GTSLFWKLTP NKQNLDYQEI
210 220 230 240 250
QGMKRGKAKV SLIMCCNADG SERLPLWIVG YAQNPRSFEQ CGIFPKSMNF
260 270 280 290 300
EWKWNGRASI SPIIMEEWLR WFDTRMQGRK VLLMLESNDT YQFGVESVRR
310 320 330 340 350
FKGGFQNTSV FRIPEKTLDI KSPFEQGIVD TFKANYRRYW LQYSLNQINI
360 370 380 390 400
LRDPLKAVNV LKAIRWMLWS WNFGVSEKTI QASFLRSGLL GPHSEAEGQG
410 420 430 440 450
MESYQKAIME IGNLISSKGS EAVKQINEYI RPSEEDETKL HQDAVDTVAA
460 470 480 490 500
EFLEERRFES DEEEDVEPQI SNVEAAMAFE VLIKYFEQHE NGDPSFVLDL
510
YRRQRVIAPN NLIA
Length:514
Mass (Da):59,865
Last modified:October 1, 2000 - v2
Checksum:iA48102EBAD0BB1E3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti168 – 1681I → M in AAB80698 (PubMed:9237993).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86754 mRNA. Translation: AAB80698.1.
CU329670 Genomic DNA. Translation: CAB16408.1.
PIRiT39217.
RefSeqiNP_594583.1. NM_001020012.2.

Genome annotation databases

EnsemblFungiiSPAC9E9.10c.1; SPAC9E9.10c.1:pep; SPAC9E9.10c.
GeneIDi2543290.
KEGGispo:SPAC9E9.10c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86754 mRNA. Translation: AAB80698.1.
CU329670 Genomic DNA. Translation: CAB16408.1.
PIRiT39217.
RefSeqiNP_594583.1. NM_001020012.2.

3D structure databases

ProteinModelPortaliO14423.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279715. 78 interactions.
MINTiMINT-4672787.

Proteomic databases

MaxQBiO14423.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC9E9.10c.1; SPAC9E9.10c.1:pep; SPAC9E9.10c.
GeneIDi2543290.
KEGGispo:SPAC9E9.10c.

Organism-specific databases

EuPathDBiFungiDB:SPAC9E9.10c.
PomBaseiSPAC9E9.10c. cbh1.

Phylogenomic databases

HOGENOMiHOG000033772.
InParanoidiO14423.
OMAiATINYEA.
OrthoDBiEOG7BZW1V.
PhylomeDBiO14423.

Miscellaneous databases

PROiO14423.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
InterProiIPR004875. DDE_SF_endonuclease_dom.
IPR009057. Homeodomain-like.
IPR006600. HTH_CenpB_DNA-bd_dom.
[Graphical view]
PfamiPF03184. DDE_1. 1 hit.
PF03221. HTH_Tnp_Tc5. 1 hit.
[Graphical view]
SMARTiSM00674. CENPB. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiPS51253. HTH_CENPB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Purification and characterization of a CENP-B homologue protein that binds to the centromeric K-type repeat DNA of Schizosaccharomyces pombe."
    Lee J.-K., Huberman J.A., Hurwitz J.
    Proc. Natl. Acad. Sci. U.S.A. 94:8427-8432(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION.
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiCBH1_SCHPO
AccessioniPrimary (citable) accession number: O14423
Secondary accession number(s): O14294
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.