O14423 (CBH1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CENP-B homolog protein 1
Short name=CBHP-1

Gene names

Name:	cbh1
Synonyms:	cbh
ORF Names:	SPAC9E9.10c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to centromeric K-type repeat DNA and ARS3002 DNA. The CBH-binding consensus sequence is Py-Pu-A-T-A-T-Py-Pu-T-A. Ref.1
Subcellular location	Nucleus. Chromosome › centromere.
Sequence similarities	Contains 1 HTH CENPB-type DNA-binding domain.

Ontologies

Keywords
Cellular component	Centromere Chromosome Nucleus
Ligand	DNA-binding
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	chromatin silencing at centromere Inferred from mutant phenotype. Source: GeneDB_Spombe histone modification Inferred from mutant phenotype. Source: GeneDB_Spombe mitotic sister chromatid segregation Inferred from genetic interaction. Source: GeneDB_Spombe
Cellular component	condensed nuclear chromosome, centromeric region Inferred from direct assay. Source: GeneDB_Spombe nuclear chromatin Non-traceable author statement. Source: GeneDB_Spombe spindle pole body Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	centromeric DNA binding Inferred from direct assay. Source: GeneDB_Spombe protein dimerization activity Non-traceable author statement. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 514	514	CENP-B homolog protein 1		PRO_0000126134
Regions
	Domain	69 – 144	76	HTH CENPB-type
	Compositional bias	421 – 493	73	Asp/Glu-rich (acidic)
Experimental info
	Sequence conflict	168	1	I → M in AAB80698. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O14423 [UniParc]. Last modified October 1, 2000. Version 2. Checksum: A48102EBAD0BB1E3	FASTA	514	59,865
	10 20 30 40 50 60 MPPIRQAVSL AEKKAIRDYY NQSAHRIPQK EVTEWFRKTY NKDLSQSTIS EILSPKYSYL 70 80 90 100 110 120 DDGSVRLGDI KKIRAPKFPL LDNAVYEWLQ QREVEGLPIS GDMIKQAATR FWSKIPAYAS 130 140 150 160 170 180 LPLPDFSNGW LDKFRRRHYI QQSAVNQALE SIKFEVFREY PVHIQEFIRP YAQKDIFCVG 190 200 210 220 230 240 GTSLFWKLTP NKQNLDYQEI QGMKRGKAKV SLIMCCNADG SERLPLWIVG YAQNPRSFEQ 250 260 270 280 290 300 CGIFPKSMNF EWKWNGRASI SPIIMEEWLR WFDTRMQGRK VLLMLESNDT YQFGVESVRR 310 320 330 340 350 360 FKGGFQNTSV FRIPEKTLDI KSPFEQGIVD TFKANYRRYW LQYSLNQINI LRDPLKAVNV 370 380 390 400 410 420 LKAIRWMLWS WNFGVSEKTI QASFLRSGLL GPHSEAEGQG MESYQKAIME IGNLISSKGS 430 440 450 460 470 480 EAVKQINEYI RPSEEDETKL HQDAVDTVAA EFLEERRFES DEEEDVEPQI SNVEAAMAFE 490 500 510 VLIKYFEQHE NGDPSFVLDL YRRQRVIAPN NLIA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification and characterization of a CENP-B homologue protein that binds to the centromeric K-type repeat DNA of Schizosaccharomyces pombe." Lee J.-K., Huberman J.A., Hurwitz J. Proc. Natl. Acad. Sci. U.S.A. 94:8427-8432(1997) [PubMed: 9237993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION. Strain: 972 / ATCC 24843.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U86754 mRNA. Translation: AAB80698.1. CU329670 Genomic DNA. Translation: CAB16408.1.
PIR	T39217.
RefSeq	NP_594583.1. NM_001020012.1.
3D structure databases
ProteinModelPortal	O14423.
ModBase	Search...
Protein-protein interaction databases
STRING	O14423.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC9E9.10c.1; SPAC9E9.10c.1:pep; SPAC9E9.10c.
GeneID	2543290.
GenomeReviews	Gene locus cbh1 in contig CU329670_GR.
KEGG	spo:SPAC9E9.10c.
NMPDR	fig|4896.1.peg.4553.
Organism-specific databases
GeneDB_Spombe	SPAC9E9.10c.
Phylogenomic databases
eggNOG	fuNOG05144.
GeneTree	EFGT00050000007452.
HOGENOM	HBG207912.
OMA	RNINTSA.
OrthoDB	EOG4DRDN0.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-002679-MONOMER.
Gene expression databases
ArrayExpress	O14423.
Family and domain databases
InterPro	IPR004875. DDE_SF_endonuclease_CENPB-like. IPR009057. Homeodomain-like. IPR012287. Homeodomain-rel. IPR006600. Pogo/CenpB/PDC2_DNA-bd_HTH. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 2 hits.
Pfam	PF03184. DDE_1. 1 hit. PF03221. HTH_Tnp_Tc5. 1 hit. [Graphical view]
SMART	SM00674. CENPB. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 2 hits.
PROSITE	PS51253. HTH_CENPB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CBH1_SCHPO

Accession

Primary (citable) accession number: O14423
Secondary accession number(s): O14294

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	October 1, 2000
Last modified:	December 14, 2011
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program