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Protein

Mitotic spindle checkpoint component mad2

Gene

mad2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. It interacts with the anaphase promoting complex/cyclosome (APC/C) thereby inhibiting APC/C-dependent proteolysis, a step required for exit from mitosis.1 Publication

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic cell cycle checkpoint inhibiting CAR assembly Source: PomBase
  • mitotic nuclear division Source: UniProtKB-KW
  • mitotic spindle assembly checkpoint Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic spindle checkpoint component mad2
Gene namesi
Name:mad2
ORF Names:SPBC20F10.06
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC20F10.06.
PomBaseiSPBC20F10.06. mad2.

Subcellular locationi

GO - Cellular componenti

  • condensed chromosome kinetochore Source: PomBase
  • condensed nuclear chromosome kinetochore Source: PomBase
  • mitotic checkpoint complex Source: PomBase
  • mitotic spindle microtubule Source: PomBase
  • mitotic spindle pole body Source: PomBase
  • nuclear chromatin Source: PomBase
  • nuclear periphery Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 203203Mitotic spindle checkpoint component mad2PRO_0000126114Add
BLAST

Proteomic databases

MaxQBiO14417.

Interactioni

Subunit structurei

Interacts with mad3 and slp1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
mad3O597672EBI-1269310,EBI-1269284
slp1P789723EBI-1269310,EBI-1252744

Protein-protein interaction databases

BioGridi277115. 107 interactions.
DIPiDIP-38037N.
IntActiO14417. 2 interactions.
MINTiMINT-4672773.

Structurei

Secondary structure

1
203
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi14 – 3320Combined sources
Helixi39 – 413Combined sources
Beta strandi42 – 476Combined sources
Beta strandi50 – 556Combined sources
Helixi58 – 7619Combined sources
Beta strandi80 – 8910Combined sources
Turni90 – 923Combined sources
Beta strandi95 – 10511Combined sources
Helixi118 – 13720Combined sources
Helixi138 – 1403Combined sources
Beta strandi149 – 15810Combined sources
Beta strandi167 – 1693Combined sources
Beta strandi179 – 1879Combined sources
Beta strandi189 – 19911Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4AEZX-ray2.30B/E/H1-203[»]
ProteinModelPortaliO14417.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 197185HORMAPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the MAD2 family.Curated
Contains 1 HORMA domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000199586.
InParanoidiO14417.
KOiK02537.
OMAiCLETNEV.
OrthoDBiEOG7FZ09D.
PhylomeDBiO14417.

Family and domain databases

Gene3Di3.30.900.10. 1 hit.
InterProiIPR003511. HORMA_dom.
IPR027097. Mad2.
[Graphical view]
PANTHERiPTHR11842:SF11. PTHR11842:SF11. 1 hit.
PfamiPF02301. HORMA. 1 hit.
[Graphical view]
SUPFAMiSSF56019. SSF56019. 1 hit.
PROSITEiPS50815. HORMA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O14417-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSVPIRTNF SLKGSSKLVS EFFEYAVNSI LFQRGIYPAE DFKVVRKYGL
60 70 80 90 100
NMLVSVDEEV KTYIRKIVSQ LHKWMFAKKI QKLILVITSK CSGEDLERWQ
110 120 130 140 150
FNVEMVDTAD QFQNIGNKED ELRVQKEIQA LIRQITATVT FLPQLEEQCT
160 170 180 190 200
FNVLVYADKD SEVPTDWVDS DPRILRDAEQ VQLRSFSTSM HKIDCQVAYR

VNP
Length:203
Mass (Da):23,583
Last modified:January 1, 1998 - v1
Checksum:iDC9D1D213450C925
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72150 Genomic RNA. Translation: AAB68597.1.
CU329671 Genomic DNA. Translation: CAA16846.1.
PIRiT39877.
RefSeqiNP_596370.1. NM_001022291.2.

Genome annotation databases

EnsemblFungiiSPBC20F10.06.1; SPBC20F10.06.1:pep; SPBC20F10.06.
GeneIDi2540589.
KEGGispo:SPBC20F10.06.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72150 Genomic RNA. Translation: AAB68597.1.
CU329671 Genomic DNA. Translation: CAA16846.1.
PIRiT39877.
RefSeqiNP_596370.1. NM_001022291.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4AEZX-ray2.30B/E/H1-203[»]
ProteinModelPortaliO14417.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277115. 107 interactions.
DIPiDIP-38037N.
IntActiO14417. 2 interactions.
MINTiMINT-4672773.

Proteomic databases

MaxQBiO14417.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC20F10.06.1; SPBC20F10.06.1:pep; SPBC20F10.06.
GeneIDi2540589.
KEGGispo:SPBC20F10.06.

Organism-specific databases

EuPathDBiFungiDB:SPBC20F10.06.
PomBaseiSPBC20F10.06. mad2.

Phylogenomic databases

HOGENOMiHOG000199586.
InParanoidiO14417.
KOiK02537.
OMAiCLETNEV.
OrthoDBiEOG7FZ09D.
PhylomeDBiO14417.

Miscellaneous databases

PROiO14417.

Family and domain databases

Gene3Di3.30.900.10. 1 hit.
InterProiIPR003511. HORMA_dom.
IPR027097. Mad2.
[Graphical view]
PANTHERiPTHR11842:SF11. PTHR11842:SF11. 1 hit.
PfamiPF02301. HORMA. 1 hit.
[Graphical view]
SUPFAMiSSF56019. SSF56019. 1 hit.
PROSITEiPS50815. HORMA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Schizosaccharomyces pombe spindle checkpoint protein mad2p blocks anaphase and genetically interacts with the anaphase-promoting complex."
    He X., Patterson T.E., Sazer S.
    Proc. Natl. Acad. Sci. U.S.A. 94:7965-7970(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Fission yeast Slp1: an effector of the Mad2-dependent spindle checkpoint."
    Kim S.H., Lin D.P., Matsumoto S., Kitazono A., Matsumoto T.
    Science 279:1045-1047(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SLP1.
  4. "Fission yeast Mad3p is required for Mad2p to inhibit the anaphase-promoting complex and localizes to kinetochores in a Bub1p-, Bub3p-, and Mph1p-dependent manner."
    Millband D.N., Hardwick K.G.
    Mol. Cell. Biol. 22:2728-2742(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MAD3.

Entry informationi

Entry nameiMAD2_SCHPO
AccessioniPrimary (citable) accession number: O14417
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 1, 1998
Last modified: June 8, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.