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Protein

Mitotic spindle checkpoint component mad2

Gene

mad2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. It interacts with the anaphase promoting complex/cyclosome (APC/C) thereby inhibiting APC/C-dependent proteolysis, a step required for exit from mitosis.1 Publication

GO - Molecular functioni

  • anaphase-promoting complex binding Source: PomBase

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • meiosis I spindle assembly checkpoint Source: PomBase
  • meiotic spindle assembly checkpoint Source: PomBase
  • mitotic cell cycle checkpoint inhibiting CAR assembly Source: PomBase
  • mitotic spindle assembly checkpoint Source: PomBase
  • negative regulation of mitotic metaphase/anaphase transition Source: PomBase
  • signal transduction involved in mitotic spindle assembly checkpoint Source: PomBase

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic spindle checkpoint component mad2
Gene namesi
Name:mad2
ORF Names:SPBC20F10.06
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC20F10.06
PomBaseiSPBC20F10.06 mad2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001261141 – 203Mitotic spindle checkpoint component mad2Add BLAST203

Proteomic databases

MaxQBiO14417
PaxDbiO14417
PRIDEiO14417

Interactioni

Subunit structurei

Interacts with mad3 and slp1.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi277115, 110 interactors
DIPiDIP-38037N
IntActiO14417, 3 interactors
STRINGi4896.SPBC20F10.06.1

Structurei

Secondary structure

1203
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 33Combined sources20
Helixi39 – 41Combined sources3
Beta strandi42 – 47Combined sources6
Beta strandi50 – 55Combined sources6
Helixi58 – 76Combined sources19
Beta strandi80 – 89Combined sources10
Turni90 – 92Combined sources3
Beta strandi95 – 105Combined sources11
Helixi118 – 137Combined sources20
Helixi138 – 140Combined sources3
Beta strandi149 – 158Combined sources10
Beta strandi167 – 169Combined sources3
Beta strandi179 – 187Combined sources9
Beta strandi189 – 199Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AEZX-ray2.30B/E/H1-203[»]
ProteinModelPortaliO14417
SMRiO14417
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 197HORMAPROSITE-ProRule annotationAdd BLAST185

Sequence similaritiesi

Belongs to the MAD2 family.Curated

Phylogenomic databases

HOGENOMiHOG000199586
InParanoidiO14417
KOiK02537
OMAiMVINDDY
OrthoDBiEOG092C4F48
PhylomeDBiO14417

Family and domain databases

Gene3Di3.30.900.10, 1 hit
InterProiView protein in InterPro
IPR003511 HORMA_dom
IPR036570 HORMA_dom_sf
IPR027097 Mad2
PANTHERiPTHR11842:SF11 PTHR11842:SF11, 1 hit
PfamiView protein in Pfam
PF02301 HORMA, 1 hit
SUPFAMiSSF56019 SSF56019, 1 hit
PROSITEiView protein in PROSITE
PS50815 HORMA, 1 hit

Sequencei

Sequence statusi: Complete.

O14417-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSVPIRTNF SLKGSSKLVS EFFEYAVNSI LFQRGIYPAE DFKVVRKYGL
60 70 80 90 100
NMLVSVDEEV KTYIRKIVSQ LHKWMFAKKI QKLILVITSK CSGEDLERWQ
110 120 130 140 150
FNVEMVDTAD QFQNIGNKED ELRVQKEIQA LIRQITATVT FLPQLEEQCT
160 170 180 190 200
FNVLVYADKD SEVPTDWVDS DPRILRDAEQ VQLRSFSTSM HKIDCQVAYR

VNP
Length:203
Mass (Da):23,583
Last modified:January 1, 1998 - v1
Checksum:iDC9D1D213450C925
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72150 Genomic RNA Translation: AAB68597.1
CU329671 Genomic DNA Translation: CAA16846.1
PIRiT39877
RefSeqiNP_596370.1, NM_001022291.2

Genome annotation databases

EnsemblFungiiSPBC20F10.06.1; SPBC20F10.06.1:pep; SPBC20F10.06
GeneIDi2540589
KEGGispo:SPBC20F10.06

Similar proteinsi

Entry informationi

Entry nameiMAD2_SCHPO
AccessioniPrimary (citable) accession number: O14417
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 1, 1998
Last modified: March 28, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health