Reviewed, UniProtKB/Swiss-Prot O14412 (GUB_ORPSP)

Names and origin

Protein names

Recommended name:
    Beta-glucanase
    EC=3.2.1.73
Alternative name(s):
    Endo-beta-1,3-1,4 glucanase
    1,3-1,4-beta-D-glucan 4-glucanohydrolase
    Lichenase

Gene names

Name:

licA

Organism

Orpinomyces sp. (strain PC-2)

Taxonomic identifier

50059 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Neocallimastigomycota › Neocallimastigomycetes › Neocallimastigales › Neocallimastigaceae › Orpinomyces

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.
Subcellular location	Secreted.
Sequence similarities	Belongs to the glycosyl hydrolase 16 family.
Biophysicochemical properties	pH dependence: Optimum pH is 5.8-6.2. Temperature dependence: Optimum temperature is 45 degrees Celsius.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	licheninase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Ref.1
	Chain	30 – 245	216	Beta-glucanase		PRO_0000011795
Sites
	Active site	134	1	Nucleophile By similarity
	Active site	138	1	Proton donor By similarity
Amino acid modifications
	Disulfide bond	63 ↔ 90		By similarity
Experimental info
	Sequence conflict	36	1	S → L AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O14412-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 08B5DF57D89F2DCC	FASTA	245	27,929
	10 20 30 40 50 60 MKSIISIAAL SVLGLISKTM AAPAPAPVPG TAWNGSHDVM DFNYHESNRF EMSNWPNGEM 70 80 90 100 110 120 FNCRWTPNND KFENGKLKLT IDRDGSGYTC GEYRTKNYYG YGMFQVNMKP IKNPGVVSSF 130 140 150 160 170 180 FTYTGPSDGT KWDEIDIEFL GYDTTKVQFN YYTNGQGHHE HIHYLGFDAS QGFHTYGFFW 190 200 210 220 230 240 ARNSITWYVD GTAVYTAYDN IPDTPGKIMM NAWNGIGVDD WLRPFNGRTN ISAYYDWVSY DAPRN

References

[1]

"Sequencing of a 1,3-1,4-beta-D-glucanase (lichenase) from the anaerobic fungus Orpinomyces strain PC-2: properties of the enzyme expressed in Escherichia coli and evidence that the gene has a bacterial origin." Chen H., Li X.-L., Ljungdahl L.G. J. Bacteriol. 179:6028-6034(1997) [PubMed: 9324248] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 30-41.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U63813 mRNA. Translation: AAD04192.1.
3D structure databases
HSSP	HSSP built from PDB template 1AJK based on UniProtKB P23904.
ModBase	Search...
Protein family/group databases
CAZy	GH16. Glycoside Hydrolase Family 16.
Family and domain databases
InterPro	IPR008264. Beta_glucanase. IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. IPR008263. Glycoside_hydrolase_16_AS. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
Pfam	PF00722. Glyco_hydro_16. 1 hit. [Graphical view]
PRINTS	PR00737. GLHYDRLASE16.
PROSITE	PS01034. GLYCOSYL_HYDROL_F16. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUB_ORPSP

Accession

Primary (citable) accession number: O14412

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 17, 2003
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)