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O14412 (GUB_ORPSP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucanase

EC=3.2.1.73
Alternative name(s):
1,3-1,4-beta-D-glucan 4-glucanohydrolase
Endo-beta-1,3-1,4 glucanase
Lichenase
Gene names
Name:licA
OrganismOrpinomyces sp. (strain PC-2)
Taxonomic identifier50059 [NCBI]
Taxonomic lineageEukaryotaFungiNeocallimastigomycotaNeocallimastigomycetesNeocallimastigalesNeocallimastigaceaeOrpinomyces

Protein attributes

Sequence length245 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

Subcellular location

Secreted.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family.

Biophysicochemical properties

pH dependence:

Optimum pH is 5.8-6.2.

Temperature dependence:

Optimum temperature is 45 degrees Celsius.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionlicheninase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Ref.1
Chain30 – 245216Beta-glucanase
PRO_0000011795

Sites

Active site1341Nucleophile By similarity
Active site1381Proton donor By similarity

Amino acid modifications

Disulfide bond63 ↔ 90 By similarity

Experimental info

Sequence conflict361S → L AA sequence Ref.1

Sequences

Sequence LengthMass (Da)Tools
O14412 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 08B5DF57D89F2DCC

FASTA24527,929
        10         20         30         40         50         60 
MKSIISIAAL SVLGLISKTM AAPAPAPVPG TAWNGSHDVM DFNYHESNRF EMSNWPNGEM 

        70         80         90        100        110        120 
FNCRWTPNND KFENGKLKLT IDRDGSGYTC GEYRTKNYYG YGMFQVNMKP IKNPGVVSSF 

       130        140        150        160        170        180 
FTYTGPSDGT KWDEIDIEFL GYDTTKVQFN YYTNGQGHHE HIHYLGFDAS QGFHTYGFFW 

       190        200        210        220        230        240 
ARNSITWYVD GTAVYTAYDN IPDTPGKIMM NAWNGIGVDD WLRPFNGRTN ISAYYDWVSY 


DAPRN 

« Hide

References

[1]"Sequencing of a 1,3-1,4-beta-D-glucanase (lichenase) from the anaerobic fungus Orpinomyces strain PC-2: properties of the enzyme expressed in Escherichia coli and evidence that the gene has a bacterial origin."
Chen H., Li X.-L., Ljungdahl L.G.
J. Bacteriol. 179:6028-6034(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 30-41.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U63813 mRNA. Translation: AAD04192.1.

3D structure databases

ProteinModelPortalO14412.
SMRO14412. Positions 55-242.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.
mycoCLAPLIC16A_ORPSP.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008264. Beta_glucanase.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PRINTSPR00737. GLHYDRLASE16.
SUPFAMSSF49899. SSF49899. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGUB_ORPSP
AccessionPrimary (citable) accession number: O14412
Entry history
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: January 1, 1998
Last modified: February 19, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries