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O14412

- GUB_ORPSP

UniProt

O14412 - GUB_ORPSP

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Protein
Beta-glucanase
Gene
licA
Organism
Orpinomyces sp. (strain PC-2)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

pH dependencei

Optimum pH is 5.8-6.2.

Temperature dependencei

Optimum temperature is 45 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei134 – 1341Nucleophile By similarity
Active sitei138 – 1381Proton donor By similarity

GO - Molecular functioni

  1. licheninase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.
mycoCLAPiLIC16A_ORPSP.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucanase (EC:3.2.1.73)
Alternative name(s):
1,3-1,4-beta-D-glucan 4-glucanohydrolase
Endo-beta-1,3-1,4 glucanase
Lichenase
Gene namesi
Name:licA
OrganismiOrpinomyces sp. (strain PC-2)
Taxonomic identifieri50059 [NCBI]
Taxonomic lineageiEukaryotaFungiNeocallimastigomycotaNeocallimastigomycetesNeocallimastigalesNeocallimastigaceaeOrpinomyces

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 29291 Publication
Add
BLAST
Chaini30 – 245216Beta-glucanase
PRO_0000011795Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi63 ↔ 90 By similarity

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliO14412.
SMRiO14412. Positions 55-242.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O14412-1 [UniParc]FASTAAdd to Basket

« Hide

MKSIISIAAL SVLGLISKTM AAPAPAPVPG TAWNGSHDVM DFNYHESNRF    50
EMSNWPNGEM FNCRWTPNND KFENGKLKLT IDRDGSGYTC GEYRTKNYYG 100
YGMFQVNMKP IKNPGVVSSF FTYTGPSDGT KWDEIDIEFL GYDTTKVQFN 150
YYTNGQGHHE HIHYLGFDAS QGFHTYGFFW ARNSITWYVD GTAVYTAYDN 200
IPDTPGKIMM NAWNGIGVDD WLRPFNGRTN ISAYYDWVSY DAPRN 245
Length:245
Mass (Da):27,929
Last modified:January 1, 1998 - v1
Checksum:i08B5DF57D89F2DCC
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 361S → L AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U63813 mRNA. Translation: AAD04192.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U63813 mRNA. Translation: AAD04192.1 .

3D structure databases

ProteinModelPortali O14412.
SMRi O14412. Positions 55-242.
ModBasei Search...

Protein family/group databases

CAZyi GH16. Glycoside Hydrolase Family 16.
mycoCLAPi LIC16A_ORPSP.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR008264. Beta_glucanase.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
[Graphical view ]
Pfami PF00722. Glyco_hydro_16. 1 hit.
[Graphical view ]
PRINTSi PR00737. GLHYDRLASE16.
SUPFAMi SSF49899. SSF49899. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Sequencing of a 1,3-1,4-beta-D-glucanase (lichenase) from the anaerobic fungus Orpinomyces strain PC-2: properties of the enzyme expressed in Escherichia coli and evidence that the gene has a bacterial origin."
    Chen H., Li X.-L., Ljungdahl L.G.
    J. Bacteriol. 179:6028-6034(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 30-41.

Entry informationi

Entry nameiGUB_ORPSP
AccessioniPrimary (citable) accession number: O14412
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: January 1, 1998
Last modified: February 19, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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