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O14405

- GUN4_HYPJE

UniProt

O14405 - GUN4_HYPJE

Protein

Endoglucanase-4

Gene

cel61a

Organism
Hypocrea jecorina (Trichoderma reesei)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. May be involved in the degradation of complex natural cellulosic substrates.1 Publication

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.2 Publications

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB
    2. cellulose binding Source: InterPro

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16504.

    Protein family/group databases

    CAZyiCBM1. Carbohydrate-Binding Module Family 1.
    GH61. Glycoside Hydrolase Family 61.
    mycoCLAPiPMO9A_TRIRE.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase-4 (EC:3.2.1.4)
    Alternative name(s):
    Cellulase IV
    Cellulase-61A
    Short name:
    Cel61A
    Endo-1,4-beta-glucanase IV
    Short name:
    EGIV
    Endoglucanase IV
    Endoglucanase-61A
    Gene namesi
    Name:cel61a
    Synonyms:egl4
    OrganismiHypocrea jecorina (Trichoderma reesei)
    Taxonomic identifieri51453 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesHypocreaceaeTrichoderma

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 344323Endoglucanase-4PRO_0000008032Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi80 – 801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi158 – 1581N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi315 ↔ 332By similarity
    Disulfide bondi326 ↔ 342By similarity

    Post-translational modificationi

    May also be O-glycosylated.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Inductioni

    By cellulose, cellobiose, lactose and sophorose.2 Publications

    Interactioni

    Protein-protein interaction databases

    STRINGi51453.JGI73643.

    Structurei

    3D structure databases

    ProteinModelPortaliO14405.
    SMRiO14405. Positions 308-343.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini307 – 34337CBM1PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni22 – 256235CatalyticSequence AnalysisAdd
    BLAST
    Regioni257 – 30751LinkerSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 61 family.Curated
    Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG126663.
    OMAiFFKIDGA.

    Family and domain databases

    InterProiIPR000254. Cellulose-bd_dom_fun.
    IPR005103. Glyco_hydro_61.
    [Graphical view]
    PfamiPF00734. CBM_1. 1 hit.
    PF03443. Glyco_hydro_61. 1 hit.
    [Graphical view]
    ProDomiPD001821. CBD_fun. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00236. fCBD. 1 hit.
    [Graphical view]
    SUPFAMiSSF57180. SSF57180. 1 hit.
    PROSITEiPS00562. CBM1_1. 1 hit.
    PS51164. CBM1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O14405-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIQKLSNLLV TALAVATGVV GHGHINDIVI NGVWYQAYDP TTFPYESNPP    50
    IVVGWTAADL DNGFVSPDAY QNPDIICHKN ATNAKGHASV KAGDTILFQW 100
    VPVPWPHPGP IVDYLANCNG DCETVDKTTL EFFKIDGVGL LSGGDPGTWA 150
    SDVLISNNNT WVVKIPDNLA PGNYVLRHEI IALHSAGQAN GAQNYPQCFN 200
    IAVSGSGSLQ PSGVLGTDLY HATDPGVLIN IYTSPLNYII PGPTVVSGLP 250
    TSVAQGSSAA TATASATVPG GGSGPTSRTT TTARTTQASS RPSSTPPATT 300
    SAPAGGPTQT LYGQCGGSGY SGPTRCAPPA TCSTLNPYYA QCLN 344
    Length:344
    Mass (Da):35,511
    Last modified:January 1, 1998 - v1
    Checksum:i7FBF1C4AB705350C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y11113 mRNA. Translation: CAA71999.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y11113 mRNA. Translation: CAA71999.1 .

    3D structure databases

    ProteinModelPortali O14405.
    SMRi O14405. Positions 308-343.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 51453.JGI73643.

    Protein family/group databases

    CAZyi CBM1. Carbohydrate-Binding Module Family 1.
    GH61. Glycoside Hydrolase Family 61.
    mycoCLAPi PMO9A_TRIRE.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi NOG126663.
    OMAi FFKIDGA.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-16504.

    Family and domain databases

    InterProi IPR000254. Cellulose-bd_dom_fun.
    IPR005103. Glyco_hydro_61.
    [Graphical view ]
    Pfami PF00734. CBM_1. 1 hit.
    PF03443. Glyco_hydro_61. 1 hit.
    [Graphical view ]
    ProDomi PD001821. CBD_fun. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00236. fCBD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57180. SSF57180. 1 hit.
    PROSITEi PS00562. CBM1_1. 1 hit.
    PS51164. CBM1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning of a Trichoderma reesei cellulase and demonstration of endoglucanase activity by expression in yeast."
      Saloheimo M., Nakari-Setaelae T., Tenkanen M., Penttilae M.
      Eur. J. Biochem. 249:584-591(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, INDUCTION.
      Strain: ATCC 56765 / Rut C-30.
    2. "Homologous expression and characterization of Cel61A (EG IV) of Trichoderma reesei."
      Karlsson J., Saloheimo M., Siika-aho M., Tenkanen M., Penttilae M., Tjerneld F.
      Eur. J. Biochem. 268:6498-6507(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.
    3. Cited for: INDUCTION.

    Entry informationi

    Entry nameiGUN4_HYPJE
    AccessioniPrimary (citable) accession number: O14405
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 27, 2002
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3