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Protein

Endoglucanase-4

Gene

cel61a

Organism
Hypocrea jecorina (Trichoderma reesei)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. May be involved in the degradation of complex natural cellulosic substrates.1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.2 Publications

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB
  2. cellulose binding Source: InterPro

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16504.

Protein family/group databases

CAZyiCBM1. Carbohydrate-Binding Module Family 1.
GH61. Glycoside Hydrolase Family 61.
mycoCLAPiPMO9A_TRIRE.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase-4 (EC:3.2.1.4)
Alternative name(s):
Cellulase IV
Cellulase-61A
Short name:
Cel61A
Endo-1,4-beta-glucanase IV
Short name:
EGIV
Endoglucanase IV
Endoglucanase-61A
Gene namesi
Name:cel61a
Synonyms:egl4
OrganismiHypocrea jecorina (Trichoderma reesei)
Taxonomic identifieri51453 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesHypocreaceaeTrichoderma

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 344323Endoglucanase-4PRO_0000008032Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi80 – 801N-linked (GlcNAc...)Sequence Analysis
Glycosylationi158 – 1581N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi315 ↔ 332By similarity
Disulfide bondi326 ↔ 342By similarity

Post-translational modificationi

May also be O-glycosylated.

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Inductioni

By cellulose, cellobiose, lactose and sophorose.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi51453.JGI73643.

Structurei

3D structure databases

ProteinModelPortaliO14405.
SMRiO14405. Positions 308-343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini307 – 34337CBM1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 256235CatalyticSequence AnalysisAdd
BLAST
Regioni257 – 30751LinkerSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 61 family.Curated
Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG126663.
OMAiICHRDAT.

Family and domain databases

InterProiIPR000254. Cellulose-bd_dom_fun.
IPR005103. Glyco_hydro_61.
[Graphical view]
PfamiPF00734. CBM_1. 1 hit.
PF03443. Glyco_hydro_61. 1 hit.
[Graphical view]
ProDomiPD001821. CBD_fun. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00236. fCBD. 1 hit.
[Graphical view]
SUPFAMiSSF57180. SSF57180. 1 hit.
PROSITEiPS00562. CBM1_1. 1 hit.
PS51164. CBM1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O14405-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQKLSNLLV TALAVATGVV GHGHINDIVI NGVWYQAYDP TTFPYESNPP
60 70 80 90 100
IVVGWTAADL DNGFVSPDAY QNPDIICHKN ATNAKGHASV KAGDTILFQW
110 120 130 140 150
VPVPWPHPGP IVDYLANCNG DCETVDKTTL EFFKIDGVGL LSGGDPGTWA
160 170 180 190 200
SDVLISNNNT WVVKIPDNLA PGNYVLRHEI IALHSAGQAN GAQNYPQCFN
210 220 230 240 250
IAVSGSGSLQ PSGVLGTDLY HATDPGVLIN IYTSPLNYII PGPTVVSGLP
260 270 280 290 300
TSVAQGSSAA TATASATVPG GGSGPTSRTT TTARTTQASS RPSSTPPATT
310 320 330 340
SAPAGGPTQT LYGQCGGSGY SGPTRCAPPA TCSTLNPYYA QCLN
Length:344
Mass (Da):35,511
Last modified:January 1, 1998 - v1
Checksum:i7FBF1C4AB705350C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11113 mRNA. Translation: CAA71999.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11113 mRNA. Translation: CAA71999.1.

3D structure databases

ProteinModelPortaliO14405.
SMRiO14405. Positions 308-343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi51453.JGI73643.

Protein family/group databases

CAZyiCBM1. Carbohydrate-Binding Module Family 1.
GH61. Glycoside Hydrolase Family 61.
mycoCLAPiPMO9A_TRIRE.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiNOG126663.
OMAiICHRDAT.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16504.

Family and domain databases

InterProiIPR000254. Cellulose-bd_dom_fun.
IPR005103. Glyco_hydro_61.
[Graphical view]
PfamiPF00734. CBM_1. 1 hit.
PF03443. Glyco_hydro_61. 1 hit.
[Graphical view]
ProDomiPD001821. CBD_fun. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00236. fCBD. 1 hit.
[Graphical view]
SUPFAMiSSF57180. SSF57180. 1 hit.
PROSITEiPS00562. CBM1_1. 1 hit.
PS51164. CBM1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA cloning of a Trichoderma reesei cellulase and demonstration of endoglucanase activity by expression in yeast."
    Saloheimo M., Nakari-Setaelae T., Tenkanen M., Penttilae M.
    Eur. J. Biochem. 249:584-591(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, INDUCTION.
    Strain: ATCC 56765 / Rut C-30.
  2. "Homologous expression and characterization of Cel61A (EG IV) of Trichoderma reesei."
    Karlsson J., Saloheimo M., Siika-aho M., Tenkanen M., Penttilae M., Tjerneld F.
    Eur. J. Biochem. 268:6498-6507(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.
  3. Cited for: INDUCTION.

Entry informationi

Entry nameiGUN4_HYPJE
AccessioniPrimary (citable) accession number: O14405
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: January 1, 1998
Last modified: April 1, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.