Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Branched-chain-amino-acid aminotransferase, mitochondrial

Gene

eca39

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.

Catalytic activityi

L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate.1 Publication
L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate.1 Publication
L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate.1 Publication

Cofactori

GO - Molecular functioni

  • L-isoleucine transaminase activity Source: PomBase
  • L-leucine transaminase activity Source: PomBase
  • L-valine transaminase activity Source: PomBase
  • pyridoxal phosphate binding Source: PomBase

GO - Biological processi

  • isoleucine biosynthetic process Source: PomBase
  • leucine biosynthetic process Source: PomBase
  • valine biosynthetic process Source: PomBase

Keywordsi

Molecular functionAminotransferase, Transferase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BRENDAi2.6.1.42 5613
ReactomeiR-SPO-70895 Branched-chain amino acid catabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Branched-chain-amino-acid aminotransferase, mitochondrial (EC:2.6.1.42)
Short name:
BCAT
Gene namesi
Name:eca39
ORF Names:SPBC428.02c, SPBC582.12c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC428.02c
PomBaseiSPBC428.02c eca39

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 47MitochondrionSequence analysisAdd BLAST47
ChainiPRO_000000128048 – 427Branched-chain-amino-acid aminotransferase, mitochondrialAdd BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei256N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

MaxQBiO14370
PaxDbiO14370
PRIDEiO14370

PTM databases

iPTMnetiO14370

Interactioni

Protein-protein interaction databases

BioGridi277349, 31 interactors
STRINGi4896.SPBC428.02c.1

Structurei

3D structure databases

ProteinModelPortaliO14370
SMRiO14370
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000276704
InParanoidiO14370
KOiK00826
OMAiAKWACFT
OrthoDBiEOG092C2KT2

Family and domain databases

CDDicd01557 BCAT_beta_family, 1 hit
InterProiView protein in InterPro
IPR001544 Aminotrans_IV
IPR018300 Aminotrans_IV_CS
IPR036038 Aminotransferase-like
IPR005786 B_amino_transII
IPR033939 BCAT_family
PfamiView protein in Pfam
PF01063 Aminotran_4, 1 hit
PIRSFiPIRSF006468 BCAT1, 1 hit
SUPFAMiSSF56752 SSF56752, 1 hit
TIGRFAMsiTIGR01123 ilvE_II, 1 hit
PROSITEiView protein in PROSITE
PS00770 AA_TRANSFER_CLASS_4, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O14370-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLMFLRRAG NIKGRNIRFA LQRGSVGYSQ QSSEACKNFL NTTQLRTMVQ
60 70 80 90 100
TAALHGPKPM DSSHIKVTNV KELKPLPEWK SLKFGENFTD HMLIMKWNRE
110 120 130 140 150
KGWSTPEIVP FGKLCFHPAS SVFHYGFECF EGMKAFRDEK GVPRLFRPIK
160 170 180 190 200
NAERMLSTGT RISLPSFDPA ELAEIIRKFV AHENRWVPDQ RGYSLYIRPT
210 220 230 240 250
FIGTDEALGV HHCDNAMLYV IASPVGPYYS SGFKAVKLCC SEESVRAWPG
260 270 280 290 300
GTGHYKLGGN YAPSVLPQKE AAKKGYAQIL WLYGDEDYIT EVGTMNCFTV
310 320 330 340 350
WINKNGEKEI ITAPLDGMIL PGVTRDSILE ICRERLAPKG WKITEGKYSM
360 370 380 390 400
KEVAQASKEG RLLEVFGAGT AALVSPVKAI NYKGTEYEIP MPEGQEAGPI
410 420
TSEISKWILD IQYGKEPNNP WSVPALP
Length:427
Mass (Da):47,855
Last modified:July 11, 2012 - v3
Checksum:i3233011479561478
GO

Sequence cautioni

The sequence AAC39352 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti236 – 243VKLCCSEE → LSFVAPKK in AAC39352 (PubMed:9483807).Curated8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88029 Genomic DNA Translation: AAC39352.1 Sequence problems.
CU329671 Genomic DNA Translation: CAB46675.2
PIRiT40454
RefSeqiNP_595180.2, NM_001021088.2

Genome annotation databases

EnsemblFungiiSPBC428.02c.1; SPBC428.02c.1:pep; SPBC428.02c
GeneIDi2540831
KEGGispo:SPBC428.02c

Similar proteinsi

Entry informationi

Entry nameiBCA1_SCHPO
AccessioniPrimary (citable) accession number: O14370
Secondary accession number(s): O94352
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 11, 2012
Last modified: March 28, 2018
This is version 135 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health