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Protein

Branched-chain-amino-acid aminotransferase, mitochondrial

Gene

eca39

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.

Catalytic activityi

L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate.1 Publication
L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate.1 Publication
L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate.1 Publication

Cofactori

GO - Molecular functioni

  • L-isoleucine transaminase activity Source: PomBase
  • L-leucine transaminase activity Source: PomBase
  • L-valine transaminase activity Source: PomBase
  • pyridoxal phosphate binding Source: PomBase

GO - Biological processi

  • aspartate biosynthetic process Source: GO_Central
  • isoleucine biosynthetic process Source: PomBase
  • leucine biosynthetic process Source: PomBase
  • valine biosynthetic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BRENDAi2.6.1.42. 5613.
ReactomeiR-SPO-70895. Branched-chain amino acid catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Branched-chain-amino-acid aminotransferase, mitochondrial (EC:2.6.1.42)
Short name:
BCAT
Gene namesi
Name:eca39
ORF Names:SPBC428.02c, SPBC582.12c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC428.02c.
PomBaseiSPBC428.02c. eca39.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • mitochondrial matrix Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 47MitochondrionSequence analysisAdd BLAST47
ChainiPRO_000000128048 – 427Branched-chain-amino-acid aminotransferase, mitochondrialAdd BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei256N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

MaxQBiO14370.
PRIDEiO14370.

Interactioni

Protein-protein interaction databases

BioGridi277349. 31 interactors.
MINTiMINT-4672713.

Structurei

3D structure databases

ProteinModelPortaliO14370.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000276704.
InParanoidiO14370.
KOiK00826.
OMAiDHHITEV.
OrthoDBiEOG092C2KT2.

Family and domain databases

CDDicd01557. BCAT_beta_family. 1 hit.
InterProiIPR001544. Aminotrans_IV.
IPR018300. Aminotrans_IV_CS.
IPR005786. B_amino_transII.
IPR033939. BCAT_family.
[Graphical view]
PANTHERiPTHR11825. PTHR11825. 1 hit.
PfamiPF01063. Aminotran_4. 1 hit.
[Graphical view]
PIRSFiPIRSF006468. BCAT1. 1 hit.
SUPFAMiSSF56752. SSF56752. 1 hit.
TIGRFAMsiTIGR01123. ilvE_II. 1 hit.
PROSITEiPS00770. AA_TRANSFER_CLASS_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O14370-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLMFLRRAG NIKGRNIRFA LQRGSVGYSQ QSSEACKNFL NTTQLRTMVQ
60 70 80 90 100
TAALHGPKPM DSSHIKVTNV KELKPLPEWK SLKFGENFTD HMLIMKWNRE
110 120 130 140 150
KGWSTPEIVP FGKLCFHPAS SVFHYGFECF EGMKAFRDEK GVPRLFRPIK
160 170 180 190 200
NAERMLSTGT RISLPSFDPA ELAEIIRKFV AHENRWVPDQ RGYSLYIRPT
210 220 230 240 250
FIGTDEALGV HHCDNAMLYV IASPVGPYYS SGFKAVKLCC SEESVRAWPG
260 270 280 290 300
GTGHYKLGGN YAPSVLPQKE AAKKGYAQIL WLYGDEDYIT EVGTMNCFTV
310 320 330 340 350
WINKNGEKEI ITAPLDGMIL PGVTRDSILE ICRERLAPKG WKITEGKYSM
360 370 380 390 400
KEVAQASKEG RLLEVFGAGT AALVSPVKAI NYKGTEYEIP MPEGQEAGPI
410 420
TSEISKWILD IQYGKEPNNP WSVPALP
Length:427
Mass (Da):47,855
Last modified:July 11, 2012 - v3
Checksum:i3233011479561478
GO

Sequence cautioni

The sequence AAC39352 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti236 – 243VKLCCSEE → LSFVAPKK in AAC39352 (PubMed:9483807).Curated8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88029 Genomic DNA. Translation: AAC39352.1. Sequence problems.
CU329671 Genomic DNA. Translation: CAB46675.2.
PIRiT40454.
RefSeqiNP_595180.2. NM_001021088.2.

Genome annotation databases

EnsemblFungiiSPBC428.02c.1; SPBC428.02c.1:pep; SPBC428.02c.
GeneIDi2540831.
KEGGispo:SPBC428.02c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88029 Genomic DNA. Translation: AAC39352.1. Sequence problems.
CU329671 Genomic DNA. Translation: CAB46675.2.
PIRiT40454.
RefSeqiNP_595180.2. NM_001021088.2.

3D structure databases

ProteinModelPortaliO14370.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277349. 31 interactors.
MINTiMINT-4672713.

Proteomic databases

MaxQBiO14370.
PRIDEiO14370.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC428.02c.1; SPBC428.02c.1:pep; SPBC428.02c.
GeneIDi2540831.
KEGGispo:SPBC428.02c.

Organism-specific databases

EuPathDBiFungiDB:SPBC428.02c.
PomBaseiSPBC428.02c. eca39.

Phylogenomic databases

HOGENOMiHOG000276704.
InParanoidiO14370.
KOiK00826.
OMAiDHHITEV.
OrthoDBiEOG092C2KT2.

Enzyme and pathway databases

BRENDAi2.6.1.42. 5613.
ReactomeiR-SPO-70895. Branched-chain amino acid catabolism.

Miscellaneous databases

PROiO14370.

Family and domain databases

CDDicd01557. BCAT_beta_family. 1 hit.
InterProiIPR001544. Aminotrans_IV.
IPR018300. Aminotrans_IV_CS.
IPR005786. B_amino_transII.
IPR033939. BCAT_family.
[Graphical view]
PANTHERiPTHR11825. PTHR11825. 1 hit.
PfamiPF01063. Aminotran_4. 1 hit.
[Graphical view]
PIRSFiPIRSF006468. BCAT1. 1 hit.
SUPFAMiSSF56752. SSF56752. 1 hit.
TIGRFAMsiTIGR01123. ilvE_II. 1 hit.
PROSITEiPS00770. AA_TRANSFER_CLASS_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBCA1_SCHPO
AccessioniPrimary (citable) accession number: O14370
Secondary accession number(s): O94352
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 11, 2012
Last modified: November 30, 2016
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.