Reviewed, UniProtKB/Swiss-Prot O14361 (PHA2_SCHPO)

Names and origin

Protein names

Recommended name:
    Putative prephenate dehydratase
      Short name=PDT
    EC=4.2.1.51

Gene names

Name:	pha2
ORF Names:	SPBC30D10.16

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Putative prephenate dehydratase required for the syntheis of L-phenylalanine. Ref.3
Catalytic activity	Prephenate = phenylpyruvate + H2O + CO2.
Pathway	Amino-acid biosynthesis; L-phenylalanine biosynthesis; phenylpyruvate from prephenate: step 1/1.
Subcellular location	Cytoplasm. Nucleus. Ref.2
Sequence similarities	Belongs to the PHA2 family. Contains 1 prephenate dehydratase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Phenylalanine biosynthesis
Cellular component	Cytoplasm Nucleus
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine biosynthetic process Inferred from mutant phenotype. Source: GeneDB_SPombe
Cellular component	cytosol Ref.2 Inferred from direct assay. Source: GeneDB_SPombe nucleus Ref.2 Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	prephenate dehydratase activity Inferred from mutant phenotype. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	Putative prephenate dehydratase		PRO_0000363369
Regions
	Domain	5 – 195	191	Prephenate dehydratase

Sequences

	Sequence		Length	Mass (Da)
	O14361-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 2C2863AA97F32DA3	FASTA	287	31,593
	10 20 30 40 50 60 MSASKIAFLG PRGTFSHQAA LLARPDSLLC SLPSFAGKLI FALKFILICF LAVLEALSSR 70 80 90 100 110 120 QVDYAVLPIE NSTNGAVIPA YDLLKGRDDI QAVGEVLVPA HHCIIGKSLE NVQKILSHPQ 130 140 150 160 170 180 AFGQCSKWIS ANVPNAEFVS VSSTSQAAAL ASKDITGTIV AISSELCAVE NQFNLLVKNI 190 200 210 220 230 240 EDDSNNRTRF LLLRSGGFQD DLSPLKEKSL LQFYLSHPKK LSAVFEVFAA HKVVITNLVV 250 260 270 280 RPSCKFPWTY IYFVECLGME KHLIDRVGKY CDTFTFMGSY TNQISYF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[3]	"Six new amino acid-auxotrophic markers for targeted gene integration and disruption in fission yeast." Ma Y., Sugiura R., Saito M., Koike A., Sio S.O., Fujita Y., Takegawa K., Kuno T. Curr. Genet. 52:97-105(2007) [PubMed: 17622533] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAB10811.1.
PIR	T40180.
RefSeq	NP_596269.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O14361.
Genome annotation databases
GeneID	2540319.
GenomeReviews	Gene locus pha2 in contig CU329671_GR.
KEGG	spo:SPBC30D10.16.
NMPDR	fig|4896.1.peg.2135.
Organism-specific databases
GeneDB_Spombe	SPBC30D10.16.
Phylogenomic databases
OMA	ATKRSEM.
Gene expression databases
ArrayExpress	O14361.
Family and domain databases
InterPro	IPR001086. Preph_deHydtase. IPR018528. Preph_deHydtase_CS. [Graphical view]
Pfam	PF00800. PDT. 1 hit. [Graphical view]
PROSITE	PS00857. PREPHENATE_DEHYDR_1. False negative. PS00858. PREPHENATE_DEHYDR_2. False negative. PS51171. PREPHENATE_DEHYDR_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHA2_SCHPO

Accession

Primary (citable) accession number: O14361

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)