O14356 (TOR1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 3-kinase tor1
Short name=PI3-kinase tor1
Short name=PI3K tor1
Short name=PtdIns-3-kinase tor1
EC=2.7.1.137

Gene names

Name:	tor1
ORF Names:	SPBC30D10.10c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	2335 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphatidylinositol 3-kinase homolog required for G1 progression and entry into stationary phase. Also required for the onset of meiosis and sporulation under nitrogen and carbon starvation conditions. Ref.2
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.
Induction	By nitrogen and/or carbon starvation, cold, osmotic and oxidative stress. Ref.2
Sequence similarities	Belongs to the PI3/PI4-kinase family. Contains 1 FAT domain. Contains 1 FATC domain. Contains 15 HEAT repeats. Contains 1 PI3K/PI4K domain.

Ontologies

Keywords
Biological process	Cell cycle
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-arginine import Inferred from mutant phenotype PubMed 15466417. Source: PomBase TOR signaling cascade Traceable author statement PubMed 18076573. Source: PomBase cell aging Traceable author statement PubMed 19875745. Source: PomBase cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cellular response to osmotic stress Inferred from mutant phenotype PubMed 11409178. Source: PomBase induction of conjugation upon nitrogen starvation Inferred from mutant phenotype Ref.2. Source: PomBase phosphatidylinositol-3-phosphate biosynthetic process Inferred from electronic annotation. Source: GOC phosphatidylinositol-mediated signaling Non-traceable author statement. Source: PomBase regulation of leucine import Inferred from mutant phenotype PubMed 15466417. Source: PomBase response to temperature stimulus Inferred from mutant phenotype PubMed 11409178. Source: PomBase
Cellular_component	TORC2 complex Inferred from direct assay PubMed 17046992PubMed 17261596. Source: PomBase cytosol Inferred from direct assay PubMed 16823372. Source: PomBase
Molecular_function	1-phosphatidylinositol-3-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW drug binding Inferred from electronic annotation. Source: InterPro protein serine/threonine kinase activity Inferred from direct assay PubMed 12805221. Source: PomBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
tel2	Q9P3W5	3	EBI-2014420,EBI-2014377

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2335	2335	Phosphatidylinositol 3-kinase tor1		PRO_0000088812
Regions
	Repeat	1 – 31	31	HEAT 1
	Repeat	164 – 201	38	HEAT 2
	Repeat	331 – 371	41	HEAT 3
	Repeat	410 – 449	40	HEAT 4
	Repeat	474 – 512	39	HEAT 5
	Repeat	522 – 560	39	HEAT 6
	Repeat	562 – 596	35	HEAT 7
	Repeat	642 – 679	38	HEAT 8
	Repeat	684 – 722	39	HEAT 9
	Repeat	728 – 766	39	HEAT 10
	Repeat	843 – 880	38	HEAT 11
	Repeat	904 – 923	20	HEAT 12
	Repeat	924 – 961	38	HEAT 13
	Repeat	964 – 1003	40	HEAT 14
	Repeat	1005 – 1042	38	HEAT 15
	Domain	1226 – 1781	556	FAT
	Domain	1987 – 2302	316	PI3K/PI4K
	Domain	2303 – 2335	33	FATC

Sequences

	Sequence		Length	Mass (Da)
	O14356 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 5DCF1CF4ABE8E9A4	FASTA	2,335	266,184
	10 20 30 40 50 60 MEYFSDLKNK NESIQLAAAD QLKEFVHSST KELSGESLAR FNNDINRRIF ELIHSHDSHE 70 80 90 100 110 120 RFGGILAIGK LIEFESEGDV TNLSRYANYL RMTLPSTDWH SMELSAKVLG HLAASGGTLA 130 140 150 160 170 180 AEFVEFEVQR AFEWLQGDRQ EQKRMAAILI IKALAQNSPT LVYLYISEIF QNLWTGLRDP 190 200 210 220 230 240 KPLIRETAAD ALGASLDVVC QREAKVQLQC FNEVLLQAEH GLRQSSVEYL HGSLLAYKEL 250 260 270 280 290 300 FEKSGSFIRE HYTEFCDLAL RLREHRDNSI RRCIVFLLPT LSEYNPKKFQ QRYLDSFMVY 310 320 330 340 350 360 LLSHIRKDKE KSLAFEAIGR IAMAVNEAMI PYLQNILKVI RDTLTAKVRE KTQYEKPVFE 370 380 390 400 410 420 CIGMLAAAVK LELLEDSRSL LGLIFSCELS VHLRQALVKM AENIPPLLAP IQERLLNMVS 430 440 450 460 470 480 QILTGKNFEI RTNDTYTPSF TNIYSAREPD QRSKSTESII LALETLGTFN FTGYSLISFI 490 500 510 520 530 540 QESVLSYLEN DNSEIRIAAA RTCCQVFARD PICRKTNPLA VESVAEVLEK LLTLGIADSD 550 560 570 580 590 600 PKIRETVLSL LDERFDRHLA HPDNIRCLFI ALNDEVFSIR EIAIIIIGRL ALYNPAHVMP 610 620 630 640 650 660 SLRKTIIQLL SDMEYSGNSR QKEESAQLLK LLVSKARTLI KPYIQSIIHV ILPKAADTSP 670 680 690 700 710 720 GVSSAIISAL GELASVEGED MPVDVRGSFM KLILVNLQDQ SSTLKRLASL KCLRKLCGRS 730 740 750 760 770 780 GYVIQPYLDY PPLLGALIGI LQSEQPTPIR REVLRTLGVL GALDPYTYLT TEEVSDDLQS 790 800 810 820 830 840 SHNNAHGVPQ ISAAQYPSLE NYAMVAVVTL IGILKDSSLS MHHSSVVQAV MHICSQMGSK 850 860 870 880 890 900 STVFLPQVVP TFLQVMQSLS ASSAEFYFQQ LTTLTSIIGP NIRDYVSDIF NLSKVFWEST 910 920 930 940 950 960 TSLLLVILEL IDAIAIALQD EFKFYLPQIL SCMLKAFSLD NTSSRSVSYK VLQSFVIFGS 970 980 990 1000 1010 1020 NIEEYMHLVL PVIIRSFERD TIPLGFRKSA LKCIAQLFQS VNFSDHASRI IHPLVRMLGK 1030 1040 1050 1060 1070 1080 SNGDLRAVIM DTLCAIVSQL GYDYSIFIPM VNKVLVSHKI SHPAYELLVS RLLKGEPLPK 1090 1100 1110 1120 1130 1140 DVVVKEFKPR PSTKPFSTQD EVLTKLPVDQ ASLKAAWESS QKLTRDDWQD WIRRISIELL 1150 1160 1170 1180 1190 1200 KESPSSALRS CSTLAGIYHP LARDLFNVSF LSCWDELTES NKKNLVKSIE LAMNAPNISV 1210 1220 1230 1240 1250 1260 EILQTLLNLA EYMEREDHTL PIPIKVISAH ASKCNVYAKA LHYTELQFVQ ETKEEVSIST 1270 1280 1290 1300 1310 1320 IESLITINNH LQQSDAAVGM LQYTKEHKQF SLKETWYEKL HRWDDALAAY EHREREGDSS 1330 1340 1350 1360 1370 1380 FEINIGKLRC YYALGDWDHL SELAQKAWVT SEQEHREAIA PLAAAAAWGL GQWNLISEYV 1390 1400 1410 1420 1430 1440 SAMDRDPQDK EFFSAISAVH LGQYNKAYGH IERHRDILVN DLSSIIGESY NRAYGIMVKS 1450 1460 1470 1480 1490 1500 QMLSELEEII DYKKNMQYEN NLDSLKKTWR KRLEGCQKNV DVWHNTLRFR ALVLSPQDSP 1510 1520 1530 1540 1550 1560 EMWIKLADLC RRSDRLKLSN QCLTYLMGRD PSNAYPLDSL KLLNPHVVYT YLKYLWATDQ 1570 1580 1590 1600 1610 1620 KNIAVSELEE FTSYLSSKHG YKMGDSSKLV DILASSSVSS EERSFLARCF HKLGKWKKSL 1630 1640 1650 1660 1670 1680 QDSVNQESVR DILNCYFYAT LFDKSWYKAW HSWALANFEV VGYYEQTEHG VTQDMYEQYI 1690 1700 1710 1720 1730 1740 VPAIKGFFHS SVLNQKNSLQ DILRLLNLWF KFGEHSDVAA AIVEGFSNVP MDTWLEVIPQ 1750 1760 1770 1780 1790 1800 LIARIHTSSS SVRASVHQLL SDIGRVHPQA LVYSLTVSSK STNPQQKHSA KSIMDSMLSH 1810 1820 1830 1840 1850 1860 SDTLVRQALL VSQELIRVAI LWHELWYEGL EEASQAYFSD HDISLMIDIV KPLHETLEKG 1870 1880 1890 1900 1910 1920 PSTLSEISFA QTFGYDLRKA RSYWQKFLQD GDPTELNQSW DLYYQVFRRI QKQLPRIKHL 1930 1940 1950 1960 1970 1980 ELQYVSPKLL DACDLELAVP GTYGHNKPVI RISHFHHTFE VISSKQRPRR LTIHGSDGKD 1990 2000 2010 2020 2030 2040 YQYVLKGHED LRQDERVMQL FGLCNTLLTT DSETFKRRLN IERYTVIPLS PNSGLLGWVP 2050 2060 2070 2080 2090 2100 HSDTLHFLIK EFRSKRNILL NLEHRMMLQM APDCDSLTLL QKLEVFEYVM ANTDGYDLYH 2110 2120 2130 2140 2150 2160 VLWLKSRSSE AWLDRRTSYT QSLAVMSMVG YILGLGDRHP SNLMMDRYSG KIIHIDFGDC 2170 2180 2190 2200 2210 2220 FEVAMHREKF PEKIPFRLTR MLINAMEVSG IQGTYKITCE LVMRVLRSNT ESLMAVLEAF 2230 2240 2250 2260 2270 2280 VYDPLINWRL MTKSSFGAST TLRPTSSSVE EKGRSYTHRA RHADYAALSE TNGVNAEGLN 2290 2300 2310 2320 2330 ERSIQVLKRV SNKLTGKDFD LKEQLPVKAQ VEKLIQQATA PENLCRCYVG WCSFW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"The fission yeast TOR homolog, tor1+, is required for the response to starvation and other stresses via a conserved serine." Weisman R., Choder M. J. Biol. Chem. 276:7027-7032(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, FUNCTION, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAB10805.1.
PIR	T40186.
RefSeq	NP_596275.1. NM_001022196.2.
3D structure databases
ProteinModelPortal	O14356.
SMR	O14356. Positions 2303-2335.
ModBase	Search...
Protein-protein interaction databases
IntAct	O14356. 7 interactions.
STRING	4896.SPBC30D10.10c-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC30D10.10c.1; SPBC30D10.10c.1:pep; SPBC30D10.10c.
GeneID	2540473.
KEGG	spo:SPBC30D10.10c.
Organism-specific databases
PomBase	SPBC30D10.10c.
Phylogenomic databases
eggNOG	COG5032.
HOGENOM	HOG000163215.
KO	K07203.
OMA	HRRENAS.
OrthoDB	EOG4ZCXCJ.
Enzyme and pathway databases
BRENDA	2.7.1.137. 5615.
Family and domain databases
Gene3D	1.10.1070.11. 3 hits. 1.25.10.10. 5 hits.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR024585. DUF3385_TOR. IPR003152. FATC. IPR011009. Kinase-like_dom. IPR000403. PI3/4_kinase_cat_dom. IPR018936. PI3/4_kinase_CS. IPR003151. PIK-rel_kinase_FAT. IPR014009. PIK_FAT. IPR009076. Rapamycin-bd_dom. IPR026683. TOR/Smg1. [Graphical view]
PANTHER	PTHR11139:SF9. PTHR11139:SF9. 1 hit.
Pfam	PF11865. DUF3385. 1 hit. PF02259. FAT. 1 hit. PF02260. FATC. 1 hit. PF00454. PI3_PI4_kinase. 1 hit. PF08771. Rapamycin_bind. 1 hit. [Graphical view]
SMART	SM00146. PI3Kc. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit. SSF47212. FRAP_FKBP12_bind. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS51189. FAT. 1 hit. PS51190. FATC. 1 hit. PS50077. HEAT_REPEAT. False negative. PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20801600.

Entry information

Entry name

TOR1_SCHPO

Accession

Primary (citable) accession number: O14356

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program