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Protein

E3 ubiquitin-protein ligase pub3

Gene

pub3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.

Miscellaneous

A cysteine residue is required for ubiquitin-thioester formation.

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei754Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-SPO-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase pub3 (EC:2.3.2.26)
Alternative name(s):
HECT-type E3 ubiquitin transferase pub3
Gene namesi
Name:pub3
ORF Names:SPBC16E9.11c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC16E9.11c
PomBaseiSPBC16E9.11c pub3

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001203341 – 786E3 ubiquitin-protein ligase pub3Add BLAST786

Proteomic databases

MaxQBiO14326
PaxDbiO14326
PRIDEiO14326

PTM databases

iPTMnetiO14326

Interactioni

Protein-protein interaction databases

BioGridi276500, 79 interactors
STRINGi4896.SPBC16E9.11c.1

Structurei

3D structure databases

ProteinModelPortaliO14326
SMRiO14326
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 90C2PROSITE-ProRule annotationAdd BLAST90
Domaini236 – 269WW 1PROSITE-ProRule annotationAdd BLAST34
Domaini306 – 339WW 2PROSITE-ProRule annotationAdd BLAST34
Domaini364 – 397WW 3PROSITE-ProRule annotationAdd BLAST34
Domaini453 – 786HECTPROSITE-ProRule annotationAdd BLAST334

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi144 – 217Thr-richAdd BLAST74

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000208451
InParanoidiO14326
KOiK10591
OrthoDBiEOG092C016J
PhylomeDBiO14326

Family and domain databases

CDDicd00078 HECTc, 1 hit
cd00201 WW, 3 hits
Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR024928 E3_ub_ligase_SMURF1
IPR000569 HECT_dom
IPR035983 Hect_E3_ubiquitin_ligase
IPR001202 WW_dom
IPR036020 WW_dom_sf
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF00632 HECT, 1 hit
PF00397 WW, 3 hits
PIRSFiPIRSF001569 E3_ub_ligase_SMURF1, 1 hit
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00119 HECTc, 1 hit
SM00456 WW, 3 hits
SUPFAMiSSF51045 SSF51045, 3 hits
SSF56204 SSF56204, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50237 HECT, 1 hit
PS01159 WW_DOMAIN_1, 3 hits
PS50020 WW_DOMAIN_2, 3 hits

Sequencei

Sequence statusi: Complete.

O14326-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQGAKRVRF YIVAADGLSK RDLFRQPDPF AILTVDGEQT HTTKVIKKSV
60 70 80 90 100
NPYWNEGFEV TVKPSSVISI RLFDQKKFKK KDQGFLGLVS FRMREVGSFR
110 120 130 140 150
SNREVSLTRP LKKSSTTNLS VLGNLVLKVA PSKIRAPAGN HSSTTANRTT
160 170 180 190 200
STPTTTTART TRTTPRPTAT TNTSNQSTSN STRNGTSAAT SNGTGTGAGT
210 220 230 240 250
GASHRSSPVT NRQTNNTSAL SNSNAHIMSS FEDQYGRLPP GWERRADSLG
260 270 280 290 300
RTYYVDHNTR TTTWTRPASS TNPVHNTSSD SQRLNHQNRH LPDDSNPSLM
310 320 330 340 350
QSDSGNDLPF GWEMRYTDTG RPYFVDHNTR TTTWVDPRNP LVRPNGGSST
360 370 380 390 400
VGSLMQPQSL SHLGPLPSGW EMRLTNSARV YFVDHNTKTT TWDDPRLPSA
410 420 430 440 450
LDQDVPQYKC DFRRKLIYFR SQPGMRPLPG QCNVKVRRDH IFEDSYAEIM
460 470 480 490 500
RYSAHDLKKR LMIRFDGEDG LDYGGLSREF FFLLSHKMFD PIYCLFEYSA
510 520 530 540 550
VDNYTLQINP HSSINPEHLN YFRFIGRVIG LAIFHRRFLD AFFVVSLYKK
560 570 580 590 600
LLRKKVSLAD MESIDAEFYR SLKWVLENDI TGILDLTFSV EEDHFGEVRT
610 620 630 640 650
VELITNGENI EVTEENKKKY VDLVTEWRVS KRVEQQFNAF YSGFVELVSP
660 670 680 690 700
DLVNVFDERE LELLIGGISD VDVEDWKSHT EYRTYIATDP VIKWFWEIIA
710 720 730 740 750
GWKNEDRSKL LQFATGTSRI PVNGFRDLQG SDGPRKFTIE KAGTPDQLPV
760 770 780
AHTCFNRLDL PDYPSKDTLH EKLSLAVENT VGFGNE
Length:786
Mass (Da):89,260
Last modified:January 1, 1998 - v1
Checksum:i57B7A859F5497B9A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti637 – 649FNAFY…VELVS → LMHFILVLLNWYP (PubMed:9501991).CuratedAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAB16903.1
AB001023 mRNA Translation: BAA19217.1
PIRiT39585
RefSeqiNP_595793.1, NM_001021694.2

Genome annotation databases

EnsemblFungiiSPBC16E9.11c.1; SPBC16E9.11c.1:pep; SPBC16E9.11c
GeneIDi2539956
KEGGispo:SPBC16E9.11c

Similar proteinsi

Entry informationi

Entry nameiPUB3_SCHPO
AccessioniPrimary (citable) accession number: O14326
Secondary accession number(s): P79055
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: January 1, 1998
Last modified: June 20, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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