Reviewed, UniProtKB/Swiss-Prot O14321 (ERG6_SCHPO)

Names and origin

Protein names

Recommended name:
    Sterol 24-C-methyltransferase
    EC=2.1.1.41
Alternative name(s):
    Delta(24)-sterol C-methyltransferase

Gene names

ORF Names:

SPBC16E9.05

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol By similarity.
Catalytic activity	S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.
Pathway	Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 1/5.
Sequence similarities	Belongs to the methyltransferase superfamily. ERG6 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	sterol 24-C-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Sterol 24-C-methyltransferase		PRO_0000124797

Sequences

	Sequence		Length	Mass (Da)
	O14321-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: FA4D3D82A1CE03D6	FASTA	378	42,867
	10 20 30 40 50 60 MSSTALLPPN TDQVLSRRLH GKAAEKKTGL AAIASKDVDE QSRKLQEYFE FWDRNHENES 70 80 90 100 110 120 EEDRARRIDG YKSVVNSYYD LATDLYEYGW SQSFHFSRFY KGEAFAQSIA RHEHYLAYRM 130 140 150 160 170 180 GIKPGSRVLD VGCGVGGPAR EITEFTGCNL VGLNNNDYQI SRCNNYAVKR NLDKKQVFVK 190 200 210 220 230 240 GDFMHMPFED NTFDYVYAIE ATVHAPSLEG VYGEIFRVLK PGGVFGVYEW VMSDDYDSSI 250 260 270 280 290 300 PKHREIAYNI EVGDGIPQMV RKCDAVEAIK KVGFNLLEED DLTDHDNPDL PWYYPLTGDI 310 320 330 340 350 360 TKCQNIWDVF TVFRTSRLGK LVTRYSVQFL EKIGVAAKGT SKVGDTLAIA QKGLIEGGET 370 HLFTPMFLMI AKKPETDA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"Identification of open reading frames in Schizosaccharomyces pombe cDNAs." Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H. DNA Res. 4:363-369(1997) [PubMed: 9501991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 55-378. Strain: PR745.
[3]	Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H. Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 55-63.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAB16897.1. D89131 mRNA. Translation: BAA13793.2.
PIR	T39579. T42375.
RefSeq	NP_595787.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2539602.
KEGG	spo:SPBC16E9.05.
NMPDR	fig|4896.1.peg.1653.
Organism-specific databases
GeneDB_Spombe	SPBC16E9.05.
Phylogenomic databases
OMA	O14321. FVKGDFM.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-003864-MON.
BRENDA	2.1.1.41. 653.
Gene expression databases
ArrayExpress	O14321.
Family and domain databases
InterPro	IPR013216. Methyltransf_11. IPR013705. Sterol_MeTrfase_C. [Graphical view]
Pfam	PF08241. Methyltransf_11. 1 hit. PF08498. Sterol_MT_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERG6_SCHPO

Accession

Primary (citable) accession number: O14321
Secondary accession number(s): P78782

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 1, 1998
Last modified:	May 5, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)