Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sterol 24-C-methyltransferase erg6

Gene

erg6

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol. Involved in the biosynthesis or ergosterol which is important for plasma membrane structure and function and for localization of plasma membrane proteins.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.

Pathwayi

GO - Molecular functioni

  1. sterol 24-C-methyltransferase activity Source: PomBase

GO - Biological processi

  1. ergosterol biosynthetic process Source: PomBase
  2. methylation Source: GO_Central
  3. protein insertion into membrane raft Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00768; UER00760.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol 24-C-methyltransferase erg6 (EC:2.1.1.41)
Alternative name(s):
Delta(24)-sterol C-methyltransferase erg6
Ergosterol biosynthesis protein 6
Gene namesi
Name:erg6
ORF Names:SPBC16E9.05
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC16E9.05.

Subcellular locationi

Nucleus 1 Publication. Endoplasmic reticulum 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. endoplasmic reticulum Source: PomBase
  3. nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 378378Sterol 24-C-methyltransferase erg6PRO_0000124797Add
BLAST

Proteomic databases

MaxQBiO14321.
PaxDbiO14321.

Interactioni

Protein-protein interaction databases

BioGridi276160. 1 interaction.
MINTiMINT-4672270.
STRINGi4896.SPBC16E9.05-1.

Structurei

3D structure databases

ProteinModelPortaliO14321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6/SMT family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0500.
HOGENOMiHOG000171097.
InParanoidiO14321.
KOiK00559.
OMAiFHFCRFS.
OrthoDBiEOG7DVDMW.
PhylomeDBiO14321.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR013216. Methyltransf_11.
IPR030384. MeTrfase_SMT.
IPR029063. SAM-dependent_MTases.
IPR013705. Sterol_MeTrfase_C.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
PF08498. Sterol_MT_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51685. SAM_MT_ERG6_SMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O14321-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTALLPPN TDQVLSRRLH GKAAEKKTGL AAIASKDVDE QSRKLQEYFE
60 70 80 90 100
FWDRNHENES EEDRARRIDG YKSVVNSYYD LATDLYEYGW SQSFHFSRFY
110 120 130 140 150
KGEAFAQSIA RHEHYLAYRM GIKPGSRVLD VGCGVGGPAR EITEFTGCNL
160 170 180 190 200
VGLNNNDYQI SRCNNYAVKR NLDKKQVFVK GDFMHMPFED NTFDYVYAIE
210 220 230 240 250
ATVHAPSLEG VYGEIFRVLK PGGVFGVYEW VMSDDYDSSI PKHREIAYNI
260 270 280 290 300
EVGDGIPQMV RKCDAVEAIK KVGFNLLEED DLTDHDNPDL PWYYPLTGDI
310 320 330 340 350
TKCQNIWDVF TVFRTSRLGK LVTRYSVQFL EKIGVAAKGT SKVGDTLAIA
360 370
QKGLIEGGET HLFTPMFLMI AKKPETDA
Length:378
Mass (Da):42,867
Last modified:January 1, 1998 - v1
Checksum:iFA4D3D82A1CE03D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAB16897.1.
D89131 mRNA. Translation: BAA13793.2.
PIRiT39579.
T42375.
RefSeqiNP_595787.1. NM_001021688.2.

Genome annotation databases

EnsemblFungiiSPBC16E9.05.1; SPBC16E9.05.1:pep; SPBC16E9.05.
GeneIDi2539602.
KEGGispo:SPBC16E9.05.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAB16897.1.
D89131 mRNA. Translation: BAA13793.2.
PIRiT39579.
T42375.
RefSeqiNP_595787.1. NM_001021688.2.

3D structure databases

ProteinModelPortaliO14321.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276160. 1 interaction.
MINTiMINT-4672270.
STRINGi4896.SPBC16E9.05-1.

Proteomic databases

MaxQBiO14321.
PaxDbiO14321.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC16E9.05.1; SPBC16E9.05.1:pep; SPBC16E9.05.
GeneIDi2539602.
KEGGispo:SPBC16E9.05.

Organism-specific databases

PomBaseiSPBC16E9.05.

Phylogenomic databases

eggNOGiCOG0500.
HOGENOMiHOG000171097.
InParanoidiO14321.
KOiK00559.
OMAiFHFCRFS.
OrthoDBiEOG7DVDMW.
PhylomeDBiO14321.

Enzyme and pathway databases

UniPathwayiUPA00768; UER00760.

Miscellaneous databases

NextBioi20800759.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR013216. Methyltransf_11.
IPR030384. MeTrfase_SMT.
IPR029063. SAM-dependent_MTases.
IPR013705. Sterol_MeTrfase_C.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
PF08498. Sterol_MT_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51685. SAM_MT_ERG6_SMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
    Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
    DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 55-378.
    Strain: PR745.
  3. Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
    Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 55-63.
  4. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. "Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe."
    Iwaki T., Iefuji H., Hiraga Y., Hosomi A., Morita T., Giga-Hama Y., Takegawa K.
    Microbiology 154:830-841(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiERG6_SCHPO
AccessioniPrimary (citable) accession number: O14321
Secondary accession number(s): P78782
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: February 4, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.