O14299 (WIS4_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MAP kinase kinase kinase wis4
EC=2.7.11.25

Alternative name(s):
MAP kinase kinase kinase wak1
MAP kinase kinase kinase wik1

Gene names

Name:	wis4
Synonyms:	wak1, wik1
ORF Names:	SPAC9G1.02

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	1401 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in a signal transduction pathway that is activated in under conditions of heat shock, oxidative stress or limited nutrition. Unlike win1, it is not activated by changes in the osmolarity of the extracellular environment. Activates the wis1 MAP kinase kinase by phosphorylation.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence CAA69030.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	activation of bipolar cell growth Inferred from mutant phenotype. Source: GeneDB_Spombe regulation of mitotic cell cycle Inferred from mutant phenotype. Source: GeneDB_Spombe stress-activated MAPK cascade Non-traceable author statement. Source: GeneDB_Spombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activity Non-traceable author statement. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1401	1401	MAP kinase kinase kinase wis4		PRO_0000086818
Regions
	Domain	1037 – 1306	270	Protein kinase
	Nucleotide binding	1043 – 1051	9	ATP By similarity
Sites
	Active site	1161	1	Proton acceptor By similarity
	Binding site	1066	1	ATP By similarity
Experimental info
	Sequence conflict	484 – 485	2	RL → SP in CAA72718. Ref.3
	Sequence conflict	1346	1	D → V in CAA69030. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O14299 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 529A989D2B627F3D	FASTA	1,401	160,538
	10 20 30 40 50 60 MGLEHTFYPA EDRFEPLLEH SEPVNFVPKE NAKSYVRQGF ASPHQSLMDN LVDSTESTKR 70 80 90 100 110 120 SENFVSHIPL TPSHSGQSEK LMSTRTSHSP YISPTMSYTN HSPANLTRNS SFNHQHYSTT 130 140 150 160 170 180 LRSPPSMRGR GIDVNSSHYP HISRPRTSSD SQKMYTRAPV DYYYIQENPY FNNIDQDSIS 190 200 210 220 230 240 DKSLPSTNQS LHHSEEDTES DNDFSESIHP EFDIDVFYKV SNILYDESDL QDPEKRERLE 250 260 270 280 290 300 WHSMLSSVLK GDVMQTEKRR LRLTEPDGHS GTYISEVWLG LQAWLHGRLN ADQAEVIRKS 310 320 330 340 350 360 REGVEPVLRE VIDFQIQDEE TTKPPLEQVT EILEKVEQCK QFYISSREME ENVPLSASKE 370 380 390 400 410 420 FNYKLNALIS WSNVMESIQV ETLVLQKWVG NDEFDLTMRT PQFNYDGVEN TSSFVERIFR 430 440 450 460 470 480 QSGLQRTFEQ RTLTTLNRII HQAKQTISEN AQAFEEMKLP TYEDKLLPLV RFPIKLLEEA 490 500 510 520 530 540 LRLRLAYAKK IKGPNFLIVD SMLDDFKIAL SVAVRIKREY IKIASPSPGW SLPTNVDEDY 550 560 570 580 590 600 DNVLLDSLKF YFKLLTLKLS SGNKNLYFKE IDFLENEWAF LNEHIYWING GDIHMAGQFS 610 620 630 640 650 660 YLSNSLLLNV HRYVESHLNG PTERTAASLT NWYSTLLKNT QIRFRKILRF SETLNSRFEN 670 680 690 700 710 720 ASDFVISEGH LPDLVNRLST TGHFLAYTAN LERDGVFVIA DHTLSENPEA LKALLFSKDI 730 740 750 760 770 780 SNLETIQQNC SYVLILCPVH PIVWKGRIEK VDVPDFSVDL KTNRVRIIAS NKREHLQAAK 790 800 810 820 830 840 SVFQSISGDL VTLAVECRSS ITRVYKEFIR LSKLCMRISS TVVDCVSAVR EACSGVNCHD 850 860 870 880 890 900 LIYHVFSFAA EFGQRILRFL SFDSYWQTKL KRKITSLAVE WISFICDECD LMDRKTFRWG 910 920 930 940 950 960 VGALEFLMLM IRGNNILLID DAMFLKIREK VGKSMAFLLT HFDVLGAKSK VAAKLQREST 970 980 990 1000 1010 1020 EVSSSPRLTS FGDVEEEALS IQLLQKETML RIDELEIERN NTLLERLAIG HVLDDSVFRN 1030 1040 1050 1060 1070 1080 RDFIKLASSF SNITIRWQQG HFVRSGMFGD VYTGVNMETG DLLAVKEIKL QDSRTFRSTV 1090 1100 1110 1120 1130 1140 DQIHNEMTVL ERLNHPNVVT YYGVEVHREK VYIFMEFCQG GSLADLLAHG RIEDENVLKV 1150 1160 1170 1180 1190 1200 YVVQLLEGLA YIHSQHILHR DIKPANILLD HRGMIKYSDF GSALYVSPPT DPEVRYEDIQ 1210 1220 1230 1240 1250 1260 PELQHLAGTP MYMAPEIILG TKKGDFGAMD IWSLGCVILE MMTGSTPWSE MDNEWAIMYH 1270 1280 1290 1300 1310 1320 VAAMHTPSIP QNEKISSLAR DFIEQCFERD PEQRPRAVDL LTHPWITDFR KKTIITMPPA 1330 1340 1350 1360 1370 1380 TITKKTSLSH TITEEKTAQL LAGRHDDSKA ETDSLAASYK EESALPVASN VGLRQPNELR 1390 1400 IDSINLPPAI VTPDTINYSV D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Multiple modes of activation of the stress-responsive MAP kinase pathway in fission yeast." Samejima I., Mackie S., Fantes P.A. EMBO J. 16:6162-6170(1997) [PubMed: 9321395] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"The Mcs4 response regulator coordinately controls the stress-activated Wak1-Wis1-Sty1 MAP kinase pathway and fission yeast cell cycle." Shieh J.C., Wilkinson M.G., Buck V., Morgan B.A., Makino K., Millar J.B.A. Genes Dev. 11:1008-1022(1997) [PubMed: 9136929] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 96-1401. Strain: 972 / ATCC 24843.
[4]	"Mcs4 mitotic catastrophe suppressor regulates the fission yeast cell cycle through the Wik1-Wis1-Spc1 kinase cascade." Shiozaki K., Shiozaki M., Russell P. Mol. Biol. Cell 8:409-419(1997) [PubMed: 9188094] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 457-543. Strain: 972 / ATCC 24843.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB11500.1. Y07750 Genomic DNA. Translation: CAA69030.1. Different initiation. Y11989 Genomic DNA. Translation: CAA72718.1. U81521 Genomic DNA. Translation: AAB39762.1.
PIR	T39225.
RefSeq	NP_593557.1. NM_001018990.1.
3D structure databases
ProteinModelPortal	O14299.
ModBase	Search...
Protein-protein interaction databases
STRING	O14299.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC9G1.02.1; SPAC9G1.02.1:pep; SPAC9G1.02.
GeneID	2542873.
GenomeReviews	Gene locus wis4 in contig CU329670_GR.
KEGG	spo:SPAC9G1.02.
NMPDR	fig|4896.1.peg.3527.
Organism-specific databases
GeneDB_Spombe	SPAC9G1.02.
Phylogenomic databases
eggNOG	fuNOG05884.
GeneTree	EFGT00050000000597.
OMA	ISHFDIM.
OrthoDB	EOG4GF6P7.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-001233-MONOMER.
BRENDA	2.7.11.25. 5615.
Gene expression databases
ArrayExpress	O14299.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR017240. MAPKKK_SSK22. IPR000719. Prot_kinase_cat_dom. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K11230.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF037579. MAPKKK_SSK22. 1 hit.
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WIS4_SCHPO

Accession

Primary (citable) accession number: O14299
Secondary accession number(s): P87062, Q92384

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program