O14275 (PPR4_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pentatricopeptide repeat-containing protein 4, mitochondrial

Gene names

Name:	ppr4
ORF Names:	SPAC8C9.06c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	931 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Predicted

General annotation (Comments)

Function	Mitochondrial RNA-binding protein that specifically activates the translation of the cox1 mRNA. Ref.3
Subcellular location	Mitochondrion Ref.2 Ref.3.
Disruption phenotype	Impairs growth on galactose. Ref.3
Sequence similarities	Contains 7 PPR (pentatricopeptide) repeats.

Ontologies

Keywords
Biological process	Protein biosynthesis Translation regulation
Cellular component	Mitochondrion
Domain	Repeat Transit peptide
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of mitochondrial translation Inferred from mutant phenotype Ref.3. Source: PomBase translation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to mitochondrial membrane Inferred from direct assay Ref.3. Source: PomBase mitochondrial inner membrane Inferred from sequence orthology. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 16	16	Mitochondrion Potential
	Chain	17 – 931	915	Pentatricopeptide repeat-containing protein 4, mitochondrial		PRO_0000317702
Regions
	Repeat	247 – 277	31	PPR 1
	Repeat	282 – 316	35	PPR 2
	Repeat	429 – 461	33	PPR 3
	Repeat	462 – 496	35	PPR 4
	Repeat	497 – 531	35	PPR 5
	Repeat	598 – 632	35	PPR 6
	Repeat	683 – 713	31	PPR 7

Sequences

	Sequence		Length	Mass (Da)
	O14275 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: D46AA3D7E40B46ED	FASTA	931	107,984
	10 20 30 40 50 60 MSKSFAYRHI WCFWRFNTPL LWFPQPLKYW PAFQQSHTFN SMSVFKNDNA IANQTTVNES 70 80 90 100 110 120 DVKRNVEKIN DIYECSNNTK SPCFPNSDSR IPLVNWKTST NPTLQVRAYM LDLLLPFFTL 130 140 150 160 170 180 DLLPFLNKVC ECCNHMLLDN PKIKQDLPFA FCYLFSSYFS YKSSRQYTCS SEEISKVFRH 190 200 210 220 230 240 LNRLGGSEYV LHLFAQIPKA LCKELTDVRT LNHPLRSIFT FCFKNISNPP NLLKLLTKLY 250 260 270 280 290 300 PRSDRANEVL YTQYLGFLTK RGDYQIAIYM FDEMYRTHHW SSFTACRLMI ESLVRQNKFE 310 320 330 340 350 360 EAISLYKKII AKRPKIARDK KILNLLLYIS TVSNRSPDAF KLALQSISNA NQIPSFDVFS 370 380 390 400 410 420 RLMSALVKYN MTEMILPLVK QYDHKFRNLY SPNIFLSIVQ ALVFCGDMVN IQRWYNMSRV 430 440 450 460 470 480 NSELSRIKHL LNCFLNSSTV SLDVSMVLEL LRDLKKKKIK VDERTLVICI TIFSRRKDLF 490 500 510 520 530 540 AMEKIHQYFS DQGIKTSNQA YAALLDAYIE AEDTEKIELY LGKIRRLGIT EDVSINRMLM 550 560 570 580 590 600 RLALDRLDWD LLEQCTKVAE RKDPEGQDYL STITMLYHVR QHELNLALRI FSGIQKPNVV 610 620 630 640 650 660 HYSIAATVLG NLNQLDQLLL LEKRMESEGK APTALSLVAF VSSYCKQGAE GLDEAKRYMS 670 680 690 700 710 720 KNFQPDKRAL LFNPRTANDM TYPPSLFSSL IKEYTSLGDI KEAKQVLSTY LEYFSKNITS 730 740 750 760 770 780 KPDIPFAIAS MRLYCSLHDT VLSRQFWDLI LQVAQQNFIT TDIAAVVDDK NIPSPSGVIP 790 800 810 820 830 840 AYKSALNVAA ETYFSFLASV NSFQELDQEW SRLEKLGFEY DDSLQNKRII WLLYDDRLDA 850 860 870 880 890 900 AIKRVYNIFL SSKRIEELIP DASSQNSIIS FFLSPDSPLY FSTLKALRDK LDMAVEDDFV 910 920 930 RISQGILVPT VKYLQTIRRD NEILFNLLAK V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[3]	"A genome wide study in fission yeast reveals nine PPR proteins that regulate mitochondrial gene expression." Kuhl I., Dujeancourt L., Gaisne M., Herbert C.J., Bonnefoy N. Nucleic Acids Res. 39:8029-8041(2011) [PubMed] [Europe PMC] [Abstract] Cited for: DOMAIN, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB16294.1.
PIR	T39143.
RefSeq	NP_594277.1. NM_001019700.2.
3D structure databases
ProteinModelPortal	O14275.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPAC8C9.06c-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC8C9.06c.1; SPAC8C9.06c.1:pep; SPAC8C9.06c.
GeneID	2543312.
KEGG	spo:SPAC8C9.06c.
Organism-specific databases
PomBase	SPAC8C9.06c.
Phylogenomic databases
eggNOG	KOG4197.
OrthoDB	EOG4WT0MS.
Family and domain databases
Gene3D	1.25.40.10. 4 hits.
InterPro	IPR002885. Pentatricopeptide_repeat. IPR011990. TPR-like_helical. [Graphical view]
Pfam	PF01535. PPR. 1 hit. [Graphical view]
PROSITE	PS51375. PPR. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	20804329.

Entry information

Entry name

PPR4_SCHPO

Accession

Primary (citable) accession number: O14275

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program