O14255 (GCS1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable mannosyl-oligosaccharide glucosidase
EC=3.2.1.106

Alternative name(s):
Processing A-glucosidase I

Gene names

ORF Names:

SPAC6G10.09

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	808 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves the distal alpha 1,2-linked glucose residue from the Glc3Man9GlcNAc2 oligosaccharide precursor highly specifically By similarity.
Catalytic activity	Exohydrolysis of the non-reducing terminal glucose residues in the mannosyl-oligosaccharide Glc3Man9GlcNAc2.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein Ref.2 Ref.3.
Sequence similarities	Belongs to the glycosyl hydrolase 63 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fungal-type cell wall biogenesis Inferred from sequence orthology. Source: PomBase oligosaccharide metabolic process Inferred from electronic annotation. Source: InterPro protein N-linked glycosylation Inferred from sequence orthology. Source: PomBase
Cellular_component	endoplasmic reticulum membrane Inferred from sequence orthology. Source: PomBase integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	mannosyl-oligosaccharide glucosidase activity Inferred from sequence orthology. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 808	808	Probable mannosyl-oligosaccharide glucosidase		PRO_0000057714
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 31	20	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	32 – 808	777	Lumenal Potential
Amino acid modifications
	Glycosylation	39	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O14255 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 31871729E6F95254	FASTA	808	92,890
	10 20 30 40 50 60 MVSDMLGGNK RWILFGLLSF LLNCVLVSCS VEDIEKAAND SFLWGPYRPN LYVGIRPKIP 70 80 90 100 110 120 DSLMTGLMWS NVDDYARFSK MRHSAEHGDD IGAFGWKHYD VRRGGQQVID DFLMGIKLET 130 140 150 160 170 180 DFVKLPEGNW ALRVHGIPLP GAPTDLTTSL FFYAYVEGEG KVGTKVNHAN HVYMEGKTPD 190 200 210 220 230 240 LGKFRIQTFN RLGEHPVSPA SVDLESMVMD KDFFAGFNVK KEGAWRTSEL ILYLLDTKMK 250 260 270 280 290 300 VISDKEGYES LKDLPPAYST LTLPNLPSEE GLQFIQKVFK GEFMFDIVFN YASSKKISEE 310 320 330 340 350 360 MISQAIDKNL QEFEEKFQAT FPLKAPYDTE KAHQIFAHTA FSNLFGNVGF FTGDSIVSKN 370 380 390 400 410 420 PIELDDEDYE FMQGFESAAG KLAEGTAFHD IERSLFTIVP SRPHFPRGFY WDEGFHLLPV 430 440 450 460 470 480 GLWDNDFSLE ILKSWFSLVN EDGWVGREQI LGEEARSKVP DEFQTQYPDI ANPPTLILAL 490 500 510 520 530 540 KGYIERLQEQ QGKLNNRFSG EGEDYSLDDL EYLRSVSISN PEKSVQFLRD LFPLLLRHYE 550 560 570 580 590 600 WFRETQKGDF ETWERECFSQ VEGYRWRGRT YQHCLASGLD DYPRAQPPST AELHVDLLSW 610 620 630 640 650 660 MTSFTRSLHF VAEFLGETEE AEKLAGYENA MLRNLEDNHW DEEVQAYCDS SVDEYDDPIN 670 680 690 700 710 720 VCHKGYVTLL PMMLGLLPAD SGRLTSLLKL IRDENELWSP YGIRSLSMND VYFGTGENYW 730 740 750 760 770 780 RGPIWINMNY LILSSLYQNY INTPGPNQNL ARSIYEELRT NVVNNVFENW RQTGIFWEQY 790 800 DPTTGKGQRT KDFTGWTSLV VNIMSENY

References

[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library." Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y. Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 381-530, SUBCELLULAR LOCATION. Strain: ATCC 38364 / 968.
[3]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB11295.1. AB028004 Genomic DNA. Translation: BAA87308.1.
PIR	T39059.
RefSeq	NP_594106.1. NM_001019530.2.
3D structure databases
ProteinModelPortal	O14255.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPAC6G10.09-1.
Protein family/group databases
CAZy	GH63. Glycoside Hydrolase Family 63.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC6G10.09.1; SPAC6G10.09.1:pep; SPAC6G10.09.
GeneID	2542534.
KEGG	spo:SPAC6G10.09.
Organism-specific databases
PomBase	SPAC6G10.09.
Phylogenomic databases
HOGENOM	HOG000201473.
KO	K01228.
OMA	GFAARVK.
OrthoDB	EOG4KM2B7.
Family and domain databases
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR004888. Glycoside_hydrolase_63. [Graphical view]
PANTHER	PTHR10412. PTHR10412. 1 hit.
Pfam	PF03200. Glyco_hydro_63. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
ProtoNet	Search...
Other
NextBio	20803587.

Entry information

Entry name

GCS1_SCHPO

Accession

Primary (citable) accession number: O14255
Secondary accession number(s): Q9US71

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program