Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable mannosyl-oligosaccharide glucosidase

Gene

SPAC6G10.09

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves the distal alpha 1,2-linked glucose residue from the Glc3Man9GlcNAc2 oligosaccharide precursor highly specifically.By similarity

Catalytic activityi

Exohydrolysis of the non-reducing terminal glucose residues in the mannosyl-oligosaccharide Glc3Man9GlcNAc2.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

ReactomeiREACT_331534. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.

Protein family/group databases

CAZyiGH63. Glycoside Hydrolase Family 63.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable mannosyl-oligosaccharide glucosidase (EC:3.2.1.106)
Alternative name(s):
Processing A-glucosidase I
Gene namesi
ORF Names:SPAC6G10.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC6G10.09.
PomBaseiSPAC6G10.09.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei12 – 3120Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini32 – 808777LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 808808Probable mannosyl-oligosaccharide glucosidasePRO_0000057714Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi39 – 391N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiO14255.

Interactioni

Protein-protein interaction databases

MINTiMINT-4671810.

Structurei

3D structure databases

ProteinModelPortaliO14255.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 63 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000201473.
InParanoidiO14255.
KOiK01228.
OMAiGFAARVK.
OrthoDBiEOG7ZD244.
PhylomeDBiO14255.

Family and domain databases

InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR004888. Glycoside_hydrolase_63.
[Graphical view]
PANTHERiPTHR10412:SF1. PTHR10412:SF1. 1 hit.
PfamiPF03200. Glyco_hydro_63. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 3 hits.

Sequencei

Sequence statusi: Complete.

O14255-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSDMLGGNK RWILFGLLSF LLNCVLVSCS VEDIEKAAND SFLWGPYRPN
60 70 80 90 100
LYVGIRPKIP DSLMTGLMWS NVDDYARFSK MRHSAEHGDD IGAFGWKHYD
110 120 130 140 150
VRRGGQQVID DFLMGIKLET DFVKLPEGNW ALRVHGIPLP GAPTDLTTSL
160 170 180 190 200
FFYAYVEGEG KVGTKVNHAN HVYMEGKTPD LGKFRIQTFN RLGEHPVSPA
210 220 230 240 250
SVDLESMVMD KDFFAGFNVK KEGAWRTSEL ILYLLDTKMK VISDKEGYES
260 270 280 290 300
LKDLPPAYST LTLPNLPSEE GLQFIQKVFK GEFMFDIVFN YASSKKISEE
310 320 330 340 350
MISQAIDKNL QEFEEKFQAT FPLKAPYDTE KAHQIFAHTA FSNLFGNVGF
360 370 380 390 400
FTGDSIVSKN PIELDDEDYE FMQGFESAAG KLAEGTAFHD IERSLFTIVP
410 420 430 440 450
SRPHFPRGFY WDEGFHLLPV GLWDNDFSLE ILKSWFSLVN EDGWVGREQI
460 470 480 490 500
LGEEARSKVP DEFQTQYPDI ANPPTLILAL KGYIERLQEQ QGKLNNRFSG
510 520 530 540 550
EGEDYSLDDL EYLRSVSISN PEKSVQFLRD LFPLLLRHYE WFRETQKGDF
560 570 580 590 600
ETWERECFSQ VEGYRWRGRT YQHCLASGLD DYPRAQPPST AELHVDLLSW
610 620 630 640 650
MTSFTRSLHF VAEFLGETEE AEKLAGYENA MLRNLEDNHW DEEVQAYCDS
660 670 680 690 700
SVDEYDDPIN VCHKGYVTLL PMMLGLLPAD SGRLTSLLKL IRDENELWSP
710 720 730 740 750
YGIRSLSMND VYFGTGENYW RGPIWINMNY LILSSLYQNY INTPGPNQNL
760 770 780 790 800
ARSIYEELRT NVVNNVFENW RQTGIFWEQY DPTTGKGQRT KDFTGWTSLV

VNIMSENY
Length:808
Mass (Da):92,890
Last modified:January 1, 1998 - v1
Checksum:i31871729E6F95254
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11295.1.
AB028004 Genomic DNA. Translation: BAA87308.1.
PIRiT39059.
RefSeqiNP_594106.1. NM_001019530.2.

Genome annotation databases

EnsemblFungiiSPAC6G10.09.1; SPAC6G10.09.1:pep; SPAC6G10.09.
GeneIDi2542534.
KEGGispo:SPAC6G10.09.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11295.1.
AB028004 Genomic DNA. Translation: BAA87308.1.
PIRiT39059.
RefSeqiNP_594106.1. NM_001019530.2.

3D structure databases

ProteinModelPortaliO14255.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4671810.

Protein family/group databases

CAZyiGH63. Glycoside Hydrolase Family 63.

Proteomic databases

MaxQBiO14255.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC6G10.09.1; SPAC6G10.09.1:pep; SPAC6G10.09.
GeneIDi2542534.
KEGGispo:SPAC6G10.09.

Organism-specific databases

EuPathDBiFungiDB:SPAC6G10.09.
PomBaseiSPAC6G10.09.

Phylogenomic databases

HOGENOMiHOG000201473.
InParanoidiO14255.
KOiK01228.
OMAiGFAARVK.
OrthoDBiEOG7ZD244.
PhylomeDBiO14255.

Enzyme and pathway databases

ReactomeiREACT_331534. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.

Miscellaneous databases

NextBioi20803587.
PROiO14255.

Family and domain databases

InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR004888. Glycoside_hydrolase_63.
[Graphical view]
PANTHERiPTHR10412:SF1. PTHR10412:SF1. 1 hit.
PfamiPF03200. Glyco_hydro_63. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 3 hits.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
    Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
    Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 381-530, SUBCELLULAR LOCATION.
    Strain: ATCC 38364 / 968.
  3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGCS1_SCHPO
AccessioniPrimary (citable) accession number: O14255
Secondary accession number(s): Q9US71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: July 22, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.