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O14255

- GCS1_SCHPO

UniProt

O14255 - GCS1_SCHPO

Protein

Probable mannosyl-oligosaccharide glucosidase

Gene

SPAC6G10.09

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Cleaves the distal alpha 1,2-linked glucose residue from the Glc3Man9GlcNAc2 oligosaccharide precursor highly specifically.By similarity

    Catalytic activityi

    Exohydrolysis of the non-reducing terminal glucose residues in the mannosyl-oligosaccharide Glc3Man9GlcNAc2.

    GO - Molecular functioni

    1. mannosyl-oligosaccharide glucosidase activity Source: PomBase

    GO - Biological processi

    1. fungal-type cell wall biogenesis Source: PomBase
    2. oligosaccharide metabolic process Source: InterPro
    3. protein N-linked glycosylation Source: PomBase

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    ReactomeiREACT_188626. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.

    Protein family/group databases

    CAZyiGH63. Glycoside Hydrolase Family 63.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable mannosyl-oligosaccharide glucosidase (EC:3.2.1.106)
    Alternative name(s):
    Processing A-glucosidase I
    Gene namesi
    ORF Names:SPAC6G10.09
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC6G10.09.

    Subcellular locationi

    Endoplasmic reticulum membrane 2 Publications; Single-pass type II membrane protein 2 Publications

    GO - Cellular componenti

    1. endoplasmic reticulum Source: PomBase
    2. endoplasmic reticulum membrane Source: PomBase
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 808808Probable mannosyl-oligosaccharide glucosidasePRO_0000057714Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi39 – 391N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiO14255.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4671810.
    STRINGi4896.SPAC6G10.09-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO14255.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini32 – 808777LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 3120Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 63 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOGENOMiHOG000201473.
    KOiK01228.
    OMAiWKAPLYT.
    OrthoDBiEOG7ZD244.
    PhylomeDBiO14255.

    Family and domain databases

    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR004888. Glycoside_hydrolase_63.
    [Graphical view]
    PANTHERiPTHR10412:SF1. PTHR10412:SF1. 1 hit.
    PfamiPF03200. Glyco_hydro_63. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    O14255-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVSDMLGGNK RWILFGLLSF LLNCVLVSCS VEDIEKAAND SFLWGPYRPN    50
    LYVGIRPKIP DSLMTGLMWS NVDDYARFSK MRHSAEHGDD IGAFGWKHYD 100
    VRRGGQQVID DFLMGIKLET DFVKLPEGNW ALRVHGIPLP GAPTDLTTSL 150
    FFYAYVEGEG KVGTKVNHAN HVYMEGKTPD LGKFRIQTFN RLGEHPVSPA 200
    SVDLESMVMD KDFFAGFNVK KEGAWRTSEL ILYLLDTKMK VISDKEGYES 250
    LKDLPPAYST LTLPNLPSEE GLQFIQKVFK GEFMFDIVFN YASSKKISEE 300
    MISQAIDKNL QEFEEKFQAT FPLKAPYDTE KAHQIFAHTA FSNLFGNVGF 350
    FTGDSIVSKN PIELDDEDYE FMQGFESAAG KLAEGTAFHD IERSLFTIVP 400
    SRPHFPRGFY WDEGFHLLPV GLWDNDFSLE ILKSWFSLVN EDGWVGREQI 450
    LGEEARSKVP DEFQTQYPDI ANPPTLILAL KGYIERLQEQ QGKLNNRFSG 500
    EGEDYSLDDL EYLRSVSISN PEKSVQFLRD LFPLLLRHYE WFRETQKGDF 550
    ETWERECFSQ VEGYRWRGRT YQHCLASGLD DYPRAQPPST AELHVDLLSW 600
    MTSFTRSLHF VAEFLGETEE AEKLAGYENA MLRNLEDNHW DEEVQAYCDS 650
    SVDEYDDPIN VCHKGYVTLL PMMLGLLPAD SGRLTSLLKL IRDENELWSP 700
    YGIRSLSMND VYFGTGENYW RGPIWINMNY LILSSLYQNY INTPGPNQNL 750
    ARSIYEELRT NVVNNVFENW RQTGIFWEQY DPTTGKGQRT KDFTGWTSLV 800
    VNIMSENY 808
    Length:808
    Mass (Da):92,890
    Last modified:January 1, 1998 - v1
    Checksum:i31871729E6F95254
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAB11295.1.
    AB028004 Genomic DNA. Translation: BAA87308.1.
    PIRiT39059.
    RefSeqiNP_594106.1. NM_001019530.2.

    Genome annotation databases

    EnsemblFungiiSPAC6G10.09.1; SPAC6G10.09.1:pep; SPAC6G10.09.
    GeneIDi2542534.
    KEGGispo:SPAC6G10.09.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAB11295.1 .
    AB028004 Genomic DNA. Translation: BAA87308.1 .
    PIRi T39059.
    RefSeqi NP_594106.1. NM_001019530.2.

    3D structure databases

    ProteinModelPortali O14255.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4671810.
    STRINGi 4896.SPAC6G10.09-1.

    Protein family/group databases

    CAZyi GH63. Glycoside Hydrolase Family 63.

    Proteomic databases

    MaxQBi O14255.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC6G10.09.1 ; SPAC6G10.09.1:pep ; SPAC6G10.09 .
    GeneIDi 2542534.
    KEGGi spo:SPAC6G10.09.

    Organism-specific databases

    PomBasei SPAC6G10.09.

    Phylogenomic databases

    HOGENOMi HOG000201473.
    KOi K01228.
    OMAi WKAPLYT.
    OrthoDBi EOG7ZD244.
    PhylomeDBi O14255.

    Enzyme and pathway databases

    Reactomei REACT_188626. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.

    Miscellaneous databases

    NextBioi 20803587.
    PROi O14255.

    Family and domain databases

    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR004888. Glycoside_hydrolase_63.
    [Graphical view ]
    PANTHERi PTHR10412:SF1. PTHR10412:SF1. 1 hit.
    Pfami PF03200. Glyco_hydro_63. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
      Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
      Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 381-530, SUBCELLULAR LOCATION.
      Strain: ATCC 38364 / 968.
    3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGCS1_SCHPO
    AccessioniPrimary (citable) accession number: O14255
    Secondary accession number(s): Q9US71
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3