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O14255

- GCS1_SCHPO

UniProt

O14255 - GCS1_SCHPO

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Protein
Probable mannosyl-oligosaccharide glucosidase
Gene
SPAC6G10.09
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Cleaves the distal alpha 1,2-linked glucose residue from the Glc3Man9GlcNAc2 oligosaccharide precursor highly specifically By similarity.

Catalytic activityi

Exohydrolysis of the non-reducing terminal glucose residues in the mannosyl-oligosaccharide Glc3Man9GlcNAc2.

GO - Molecular functioni

  1. mannosyl-oligosaccharide glucosidase activity Source: PomBase
Complete GO annotation...

GO - Biological processi

  1. fungal-type cell wall biogenesis Source: PomBase
  2. oligosaccharide metabolic process Source: InterPro
  3. protein N-linked glycosylation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

ReactomeiREACT_188626. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.

Protein family/group databases

CAZyiGH63. Glycoside Hydrolase Family 63.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable mannosyl-oligosaccharide glucosidase (EC:3.2.1.106)
Alternative name(s):
Processing A-glucosidase I
Gene namesi
ORF Names:SPAC6G10.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC6G10.09.

Subcellular locationi

Endoplasmic reticulum membrane; Single-pass type II membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei12 – 3120Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini32 – 808777Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: PomBase
  2. endoplasmic reticulum membrane Source: PomBase
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 808808Probable mannosyl-oligosaccharide glucosidase
PRO_0000057714Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi39 – 391N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiO14255.

Interactioni

Protein-protein interaction databases

MINTiMINT-4671810.
STRINGi4896.SPAC6G10.09-1.

Structurei

3D structure databases

ProteinModelPortaliO14255.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000201473.
KOiK01228.
OMAiWKAPLYT.
OrthoDBiEOG7ZD244.
PhylomeDBiO14255.

Family and domain databases

InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR004888. Glycoside_hydrolase_63.
[Graphical view]
PANTHERiPTHR10412:SF1. PTHR10412:SF1. 1 hit.
PfamiPF03200. Glyco_hydro_63. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 3 hits.

Sequencei

Sequence statusi: Complete.

O14255-1 [UniParc]FASTAAdd to Basket

« Hide

MVSDMLGGNK RWILFGLLSF LLNCVLVSCS VEDIEKAAND SFLWGPYRPN    50
LYVGIRPKIP DSLMTGLMWS NVDDYARFSK MRHSAEHGDD IGAFGWKHYD 100
VRRGGQQVID DFLMGIKLET DFVKLPEGNW ALRVHGIPLP GAPTDLTTSL 150
FFYAYVEGEG KVGTKVNHAN HVYMEGKTPD LGKFRIQTFN RLGEHPVSPA 200
SVDLESMVMD KDFFAGFNVK KEGAWRTSEL ILYLLDTKMK VISDKEGYES 250
LKDLPPAYST LTLPNLPSEE GLQFIQKVFK GEFMFDIVFN YASSKKISEE 300
MISQAIDKNL QEFEEKFQAT FPLKAPYDTE KAHQIFAHTA FSNLFGNVGF 350
FTGDSIVSKN PIELDDEDYE FMQGFESAAG KLAEGTAFHD IERSLFTIVP 400
SRPHFPRGFY WDEGFHLLPV GLWDNDFSLE ILKSWFSLVN EDGWVGREQI 450
LGEEARSKVP DEFQTQYPDI ANPPTLILAL KGYIERLQEQ QGKLNNRFSG 500
EGEDYSLDDL EYLRSVSISN PEKSVQFLRD LFPLLLRHYE WFRETQKGDF 550
ETWERECFSQ VEGYRWRGRT YQHCLASGLD DYPRAQPPST AELHVDLLSW 600
MTSFTRSLHF VAEFLGETEE AEKLAGYENA MLRNLEDNHW DEEVQAYCDS 650
SVDEYDDPIN VCHKGYVTLL PMMLGLLPAD SGRLTSLLKL IRDENELWSP 700
YGIRSLSMND VYFGTGENYW RGPIWINMNY LILSSLYQNY INTPGPNQNL 750
ARSIYEELRT NVVNNVFENW RQTGIFWEQY DPTTGKGQRT KDFTGWTSLV 800
VNIMSENY 808
Length:808
Mass (Da):92,890
Last modified:January 1, 1998 - v1
Checksum:i31871729E6F95254
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329670 Genomic DNA. Translation: CAB11295.1.
AB028004 Genomic DNA. Translation: BAA87308.1.
PIRiT39059.
RefSeqiNP_594106.1. NM_001019530.2.

Genome annotation databases

EnsemblFungiiSPAC6G10.09.1; SPAC6G10.09.1:pep; SPAC6G10.09.
GeneIDi2542534.
KEGGispo:SPAC6G10.09.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329670 Genomic DNA. Translation: CAB11295.1 .
AB028004 Genomic DNA. Translation: BAA87308.1 .
PIRi T39059.
RefSeqi NP_594106.1. NM_001019530.2.

3D structure databases

ProteinModelPortali O14255.
ModBasei Search...

Protein-protein interaction databases

MINTi MINT-4671810.
STRINGi 4896.SPAC6G10.09-1.

Protein family/group databases

CAZyi GH63. Glycoside Hydrolase Family 63.

Proteomic databases

MaxQBi O14255.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC6G10.09.1 ; SPAC6G10.09.1:pep ; SPAC6G10.09 .
GeneIDi 2542534.
KEGGi spo:SPAC6G10.09.

Organism-specific databases

PomBasei SPAC6G10.09.

Phylogenomic databases

HOGENOMi HOG000201473.
KOi K01228.
OMAi WKAPLYT.
OrthoDBi EOG7ZD244.
PhylomeDBi O14255.

Enzyme and pathway databases

Reactomei REACT_188626. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.

Miscellaneous databases

NextBioi 20803587.
PROi O14255.

Family and domain databases

InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR004888. Glycoside_hydrolase_63.
[Graphical view ]
PANTHERi PTHR10412:SF1. PTHR10412:SF1. 1 hit.
Pfami PF03200. Glyco_hydro_63. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 3 hits.
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
    Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
    Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 381-530, SUBCELLULAR LOCATION.
    Strain: ATCC 38364 / 968.
  3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGCS1_SCHPO
AccessioniPrimary (citable) accession number: O14255
Secondary accession number(s): Q9US71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi