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Protein

Probable mannosyl-oligosaccharide glucosidase

Gene

SPAC6G10.09

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cleaves the distal alpha 1,2-linked glucose residue from the Glc3Man9GlcNAc2 oligosaccharide precursor highly specifically.By similarity

Catalytic activityi

Glc3Man9GlcNAc2-[protein] + H2O = Glc2Man9GlcNAc2-[protein] + beta-D-glucopyranose.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

ReactomeiR-SPO-532668 N-glycan trimming in the ER and Calnexin/Calreticulin cycle

Protein family/group databases

CAZyiGH63 Glycoside Hydrolase Family 63

Names & Taxonomyi

Protein namesi
Recommended name:
Probable mannosyl-oligosaccharide glucosidase (EC:3.2.1.106)
Alternative name(s):
Processing A-glucosidase I
Gene namesi
ORF Names:SPAC6G10.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC6G10.09
PomBaseiSPAC6G10.09

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 31Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST20
Topological domaini32 – 808LumenalSequence analysisAdd BLAST777

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000577141 – 808Probable mannosyl-oligosaccharide glucosidaseAdd BLAST808

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi39N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiO14255
PaxDbiO14255
PRIDEiO14255

PTM databases

iPTMnetiO14255

Interactioni

Protein-protein interaction databases

STRINGi4896.SPAC6G10.09.1

Structurei

3D structure databases

ProteinModelPortaliO14255
SMRiO14255
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 63 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000201473
InParanoidiO14255
KOiK01228
OMAiENYWRSP
OrthoDBiEOG092C0MZS
PhylomeDBiO14255

Family and domain databases

Gene3Di1.50.10.10, 1 hit
2.70.98.110, 1 hit
InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR012341 6hp_glycosidase-like_sf
IPR031335 Glyco_hydro_63_C
IPR031631 Glyco_hydro_63N
IPR038518 Glyco_hydro_63N_sf
IPR004888 Glycoside_hydrolase_63
PANTHERiPTHR10412 PTHR10412, 1 hit
PfamiView protein in Pfam
PF03200 Glyco_hydro_63, 1 hit
PF16923 Glyco_hydro_63N, 1 hit
SUPFAMiSSF48208 SSF48208, 3 hits

Sequencei

Sequence statusi: Complete.

O14255-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSDMLGGNK RWILFGLLSF LLNCVLVSCS VEDIEKAAND SFLWGPYRPN
60 70 80 90 100
LYVGIRPKIP DSLMTGLMWS NVDDYARFSK MRHSAEHGDD IGAFGWKHYD
110 120 130 140 150
VRRGGQQVID DFLMGIKLET DFVKLPEGNW ALRVHGIPLP GAPTDLTTSL
160 170 180 190 200
FFYAYVEGEG KVGTKVNHAN HVYMEGKTPD LGKFRIQTFN RLGEHPVSPA
210 220 230 240 250
SVDLESMVMD KDFFAGFNVK KEGAWRTSEL ILYLLDTKMK VISDKEGYES
260 270 280 290 300
LKDLPPAYST LTLPNLPSEE GLQFIQKVFK GEFMFDIVFN YASSKKISEE
310 320 330 340 350
MISQAIDKNL QEFEEKFQAT FPLKAPYDTE KAHQIFAHTA FSNLFGNVGF
360 370 380 390 400
FTGDSIVSKN PIELDDEDYE FMQGFESAAG KLAEGTAFHD IERSLFTIVP
410 420 430 440 450
SRPHFPRGFY WDEGFHLLPV GLWDNDFSLE ILKSWFSLVN EDGWVGREQI
460 470 480 490 500
LGEEARSKVP DEFQTQYPDI ANPPTLILAL KGYIERLQEQ QGKLNNRFSG
510 520 530 540 550
EGEDYSLDDL EYLRSVSISN PEKSVQFLRD LFPLLLRHYE WFRETQKGDF
560 570 580 590 600
ETWERECFSQ VEGYRWRGRT YQHCLASGLD DYPRAQPPST AELHVDLLSW
610 620 630 640 650
MTSFTRSLHF VAEFLGETEE AEKLAGYENA MLRNLEDNHW DEEVQAYCDS
660 670 680 690 700
SVDEYDDPIN VCHKGYVTLL PMMLGLLPAD SGRLTSLLKL IRDENELWSP
710 720 730 740 750
YGIRSLSMND VYFGTGENYW RGPIWINMNY LILSSLYQNY INTPGPNQNL
760 770 780 790 800
ARSIYEELRT NVVNNVFENW RQTGIFWEQY DPTTGKGQRT KDFTGWTSLV

VNIMSENY
Length:808
Mass (Da):92,890
Last modified:January 1, 1998 - v1
Checksum:i31871729E6F95254
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAB11295.1
AB028004 Genomic DNA Translation: BAA87308.1
PIRiT39059
RefSeqiNP_594106.1, NM_001019530.2

Genome annotation databases

EnsemblFungiiSPAC6G10.09.1; SPAC6G10.09.1:pep; SPAC6G10.09
GeneIDi2542534
KEGGispo:SPAC6G10.09

Similar proteinsi

Entry informationi

Entry nameiGCS1_SCHPO
AccessioniPrimary (citable) accession number: O14255
Secondary accession number(s): Q9US71
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health