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O14255 (GCS1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable mannosyl-oligosaccharide glucosidase

EC=3.2.1.106
Alternative name(s):
Processing A-glucosidase I
Gene names
ORF Names:SPAC6G10.09
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length808 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cleaves the distal alpha 1,2-linked glucose residue from the Glc3Man9GlcNAc2 oligosaccharide precursor highly specifically By similarity.

Catalytic activity

Exohydrolysis of the non-reducing terminal glucose residues in the mannosyl-oligosaccharide Glc3Man9GlcNAc2.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein Ref.2 Ref.3.

Sequence similarities

Belongs to the glycosyl hydrolase 63 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 808808Probable mannosyl-oligosaccharide glucosidase
PRO_0000057714

Regions

Topological domain1 – 1111Cytoplasmic Potential
Transmembrane12 – 3120Helical; Signal-anchor for type II membrane protein; Potential
Topological domain32 – 808777Lumenal Potential

Amino acid modifications

Glycosylation391N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
O14255 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 31871729E6F95254

FASTA80892,890
        10         20         30         40         50         60 
MVSDMLGGNK RWILFGLLSF LLNCVLVSCS VEDIEKAAND SFLWGPYRPN LYVGIRPKIP 

        70         80         90        100        110        120 
DSLMTGLMWS NVDDYARFSK MRHSAEHGDD IGAFGWKHYD VRRGGQQVID DFLMGIKLET 

       130        140        150        160        170        180 
DFVKLPEGNW ALRVHGIPLP GAPTDLTTSL FFYAYVEGEG KVGTKVNHAN HVYMEGKTPD 

       190        200        210        220        230        240 
LGKFRIQTFN RLGEHPVSPA SVDLESMVMD KDFFAGFNVK KEGAWRTSEL ILYLLDTKMK 

       250        260        270        280        290        300 
VISDKEGYES LKDLPPAYST LTLPNLPSEE GLQFIQKVFK GEFMFDIVFN YASSKKISEE 

       310        320        330        340        350        360 
MISQAIDKNL QEFEEKFQAT FPLKAPYDTE KAHQIFAHTA FSNLFGNVGF FTGDSIVSKN 

       370        380        390        400        410        420 
PIELDDEDYE FMQGFESAAG KLAEGTAFHD IERSLFTIVP SRPHFPRGFY WDEGFHLLPV 

       430        440        450        460        470        480 
GLWDNDFSLE ILKSWFSLVN EDGWVGREQI LGEEARSKVP DEFQTQYPDI ANPPTLILAL 

       490        500        510        520        530        540 
KGYIERLQEQ QGKLNNRFSG EGEDYSLDDL EYLRSVSISN PEKSVQFLRD LFPLLLRHYE 

       550        560        570        580        590        600 
WFRETQKGDF ETWERECFSQ VEGYRWRGRT YQHCLASGLD DYPRAQPPST AELHVDLLSW 

       610        620        630        640        650        660 
MTSFTRSLHF VAEFLGETEE AEKLAGYENA MLRNLEDNHW DEEVQAYCDS SVDEYDDPIN 

       670        680        690        700        710        720 
VCHKGYVTLL PMMLGLLPAD SGRLTSLLKL IRDENELWSP YGIRSLSMND VYFGTGENYW 

       730        740        750        760        770        780 
RGPIWINMNY LILSSLYQNY INTPGPNQNL ARSIYEELRT NVVNNVFENW RQTGIFWEQY 

       790        800 
DPTTGKGQRT KDFTGWTSLV VNIMSENY 

« Hide

References

[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 381-530, SUBCELLULAR LOCATION.
Strain: ATCC 38364 / 968.
[3]"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU329670 Genomic DNA. Translation: CAB11295.1.
AB028004 Genomic DNA. Translation: BAA87308.1.
PIRT39059.
RefSeqNP_594106.1. NM_001019530.2.

3D structure databases

ProteinModelPortalO14255.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4671810.
STRING4896.SPAC6G10.09-1.

Protein family/group databases

CAZyGH63. Glycoside Hydrolase Family 63.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPAC6G10.09.1; SPAC6G10.09.1:pep; SPAC6G10.09.
GeneID2542534.
KEGGspo:SPAC6G10.09.

Organism-specific databases

PomBaseSPAC6G10.09.

Phylogenomic databases

HOGENOMHOG000201473.
KOK01228.
OMASTIDIAF.
OrthoDBEOG7ZD244.
PhylomeDBO14255.

Family and domain databases

InterProIPR008928. 6-hairpin_glycosidase-like.
IPR004888. Glycoside_hydrolase_63.
[Graphical view]
PANTHERPTHR10412. PTHR10412. 1 hit.
PfamPF03200. Glyco_hydro_63. 1 hit.
[Graphical view]
SUPFAMSSF48208. SSF48208. 3 hits.
ProtoNetSearch...

Other

NextBio20803587.
PROO14255.

Entry information

Entry nameGCS1_SCHPO
AccessionPrimary (citable) accession number: O14255
Secondary accession number(s): Q9US71
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries