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O14254

- IDHP_SCHPO

UniProt

O14254 - IDHP_SCHPO

Protein

Probable isocitrate dehydrogenase [NADP], mitochondrial

Gene

idp1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (18 Apr 2012)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei108 – 1081SubstrateBy similarity
    Binding sitei113 – 1131NADPBy similarity
    Binding sitei140 – 1401SubstrateBy similarity
    Binding sitei163 – 1631SubstrateBy similarity
    Sitei170 – 1701Critical for catalysisBy similarity
    Sitei243 – 2431Critical for catalysisBy similarity
    Metal bindingi283 – 2831Magnesium or manganeseBy similarity
    Binding sitei291 – 2911NADPBy similarity
    Metal bindingi306 – 3061Magnesium or manganeseBy similarity
    Binding sitei359 – 3591NADP; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi106 – 1083NADPBy similarity
    Nucleotide bindingi341 – 3466NADPBy similarity

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NADP+) activity Source: PomBase
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. glutamate biosynthetic process Source: PomBase
    2. isocitrate metabolic process Source: PomBase
    3. NADPH regeneration Source: PomBase
    4. tricarboxylic acid cycle Source: PomBase

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NADP

    Enzyme and pathway databases

    ReactomeiREACT_213505. Citric acid cycle (TCA cycle).
    REACT_213879. NADPH regeneration.
    REACT_215537. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable isocitrate dehydrogenase [NADP], mitochondrial (EC:1.1.1.42)
    Short name:
    IDH
    Alternative name(s):
    IDP
    NADP(+)-specific ICDH
    Oxalosuccinate decarboxylase
    Gene namesi
    Name:idp1
    ORF Names:SPAC6G10.08
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC6G10.08.

    Subcellular locationi

    Mitochondrion 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: PomBase
    2. mitochondrion Source: PomBase

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2626MitochondrionAdd
    BLAST
    Chaini27 – 439413Probable isocitrate dehydrogenase [NADP], mitochondrialPRO_0000014425Add
    BLAST

    Proteomic databases

    MaxQBiO14254.
    PaxDbiO14254.
    PRIDEiO14254.

    Interactioni

    Protein-protein interaction databases

    BioGridi279052. 72 interactions.
    MINTiMINT-4671801.
    STRINGi4896.SPAC6G10.08-1.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1317Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0538.
    HOGENOMiHOG000019858.
    KOiK00031.
    OrthoDBiEOG7G4QQD.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11822. PTHR11822. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O14254-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLEVRAAVKA PFKLAAAGRG FMNMRMASSK SFQKITVKNP VVEMDGDEMT    50
    RVIWKIIREK LVLPYMDIKL DYYDLGIEAR DKTNDQITVD AAKAILKNDV 100
    GIKCATITPD EARVKEYNLK KMWKSPNGTI RNILNGTVFR EPILIKNIPK 150
    YIPGWTNPIC IGRHAFGDQY KSTDLVASGP GKLELSFTPK GNPSAKETYN 200
    VYEFNGSGVA MSMYNTDDSI RGFAHSSFQM ALQKKMPLYL STKNTILKKY 250
    DGRFKDTFQE VYESDYKQKF EELGLWYQHR LIDDMVAQAI KSNGGFVWAC 300
    KNYDGDVMSD VVAQAYGSLG LMTSVLIHPN GRTFESEAAH GTVQRHYMQY 350
    LKGKKTSTNS IASIFAWTRG LAHRGRLDGN ERLVKFANAL EHACVRCVEK 400
    GIMTKDLYLL SKSPNGYVDT FEFLDAVKSE LDSELVNIA 439
    Length:439
    Mass (Da):49,479
    Last modified:April 18, 2012 - v2
    Checksum:iCF68839E7C423F5D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAB11294.2.
    PIRiT39058.
    RefSeqiNP_594105.2. NM_001019529.2.

    Genome annotation databases

    EnsemblFungiiSPAC6G10.08.1; SPAC6G10.08.1:pep; SPAC6G10.08.
    GeneIDi2542598.
    KEGGispo:SPAC6G10.08.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAB11294.2 .
    PIRi T39058.
    RefSeqi NP_594105.2. NM_001019529.2.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 279052. 72 interactions.
    MINTi MINT-4671801.
    STRINGi 4896.SPAC6G10.08-1.

    Proteomic databases

    MaxQBi O14254.
    PaxDbi O14254.
    PRIDEi O14254.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC6G10.08.1 ; SPAC6G10.08.1:pep ; SPAC6G10.08 .
    GeneIDi 2542598.
    KEGGi spo:SPAC6G10.08.

    Organism-specific databases

    PomBasei SPAC6G10.08.

    Phylogenomic databases

    eggNOGi COG0538.
    HOGENOMi HOG000019858.
    KOi K00031.
    OrthoDBi EOG7G4QQD.

    Enzyme and pathway databases

    Reactomei REACT_213505. Citric acid cycle (TCA cycle).
    REACT_213879. NADPH regeneration.
    REACT_215537. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.

    Miscellaneous databases

    NextBioi 20803647.
    PROi O14254.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11822. PTHR11822. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "Comparative functional genomics of the fission yeasts."
      Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N., Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y., Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K.
      , Bayne E.H., Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G., French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A., Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P., Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R., Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J., Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W., Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.
      Science 332:930-936(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVISION OF GENE MODEL.
    3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiIDHP_SCHPO
    AccessioniPrimary (citable) accession number: O14254
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: April 18, 2012
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3