Reviewed,
UniProtKB/Swiss-Prot O14254 (IDHP_SCHPO)
Last modified
June 16, 2009.
Version 65.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Probable isocitrate dehydrogenase [NADP], mitochondrial Short name=IDH EC=1.1.1.42 Alternative name(s): Oxalosuccinate decarboxylase NADP(+)-specific ICDH IDP | ||||
| Gene names |
| ||||
| Organism | Schizosaccharomyces pombe (Fission yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4896 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces |
Protein attributes
| Sequence length | 418 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Catalytic activity | Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. Oxalosuccinate + NADP+ = 2-oxoglutarate + CO2 + NADPH. |
| Cofactor | Binds 1 magnesium or manganese ion per subunit By similarity. |
| Subcellular location | Mitochondrion By similarity. |
| Sequence similarities | Belongs to the isocitrate and isopropylmalate dehydrogenases family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Tricarboxylic acid cycle |
| Cellular component | Mitochondrion |
| Domain | Transit peptide |
| Ligand | Magnesium Manganese Metal-binding NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | isocitrate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycleInferred by curator. Source: GeneDB_SPombe |
| Cellular component | mitochondrion Inferred from direct assay. Source: GeneDB_SPombe |
| Molecular function | NAD or NADH binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NADP+) activityInferred from electronic annotation. Source: EC magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW manganese ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – ? | Mitochondrion | |||||||
| Chain | ? – 418 | Probable isocitrate dehydrogenase [NADP], mitochondrial | PRO_0000014425 | ||||||
Regions | |||||||||
| Nucleotide binding | 85 – 87 | 3 | NADP By similarity | ||||||
| Nucleotide binding | 320 – 325 | 6 | NADP By similarity | ||||||
| Region | 104 – 110 | 7 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Metal binding | 262 | 1 | Magnesium or manganese By similarity | ||||||
| Metal binding | 285 | 1 | Magnesium or manganese By similarity | ||||||
| Binding site | 87 | 1 | Substrate By similarity | ||||||
| Binding site | 92 | 1 | NADP By similarity | ||||||
| Binding site | 119 | 1 | Substrate By similarity | ||||||
| Binding site | 142 | 1 | Substrate By similarity | ||||||
| Binding site | 270 | 1 | NADP By similarity | ||||||
| Binding site | 338 | 1 | NADP; via amide nitrogen and carbonyl oxygen By similarity | ||||||
| Site | 149 | 1 | Critical for catalysis By similarity | ||||||
| Site | 222 | 1 | Critical for catalysis By similarity | ||||||
Sequences
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References
| [1] | "The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. Nurse P.Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972. |
Cross-references
Sequence databases | |
|---|---|
| CU329670 Genomic DNA. Translation: CAB11294.1. | |
| PIR | T39058. |
| RefSeq | NP_594105.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1LWD based on UniProtKB P33198. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 2542598. |
| KEGG | spo:SPAC6G10.08. |
| NMPDR | fig|4896.1.peg.4075. |
Organism-specific databases | |
| GeneDB_Spombe | SPAC6G10.08. |
Phylogenomic databases | |
| OMA | O14254. GGMLWAC. |
Enzyme and pathway databases | |
| BioCyc | SPOM-XXX-01:SPOM-XXX-01-001728-MON. |
| BRENDA | 1.1.1.42. 653. |
Gene expression databases | |
| ArrayExpress | O14254. |
Family and domain databases | |
| InterPro | IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004790. Isocitrate_DH_NADP-dep_euk. [Graphical view] |
| Gene3D | G3DSA:3.40.718.10. IDH_IMDH. 1 hit. |
| PANTHER | PTHR11822. IDH_NADP_euk. 1 hit. |
| Pfam | PF00180. Iso_dh. 1 hit. [Graphical view] |
| PIRSF | PIRSF000108. IDH_NADP. 1 hit. |
| TIGRFAMs | TIGR00127. nadp_idh_euk. 1 hit. |
| PROSITE | PS00470. IDH_IMDH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | IDHP_SCHPO | ||||||||
| Accession | Primary (citable) accession number: O14254 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| Schizosaccharomyces pombe Schizosaccharomyces pombe: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

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